Restriction map for pF1KSDA1260
 (Linear) (Six-base) MAPSORT of: KIAA1260.seq  Check: 5916  from: 1  to: 2448



KIAA1260.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2272    2448 
   Size:       2272     176
 
AatII G_ACGT'C

Cuts at:      0    1043    1550    2448 
   Size:       1043     507     898

  Fragments arranged by size: 

                1043     898     507
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1150    1250    1506    2376    2448 
   Size:       1150     100     256     870      72

  Fragments arranged by size: 

                1150     870     256     100      72
 
AgeI A'CCGG_T

Cuts at:      0     637    2448 
   Size:        637    1811
 
AleI CACnn'nnGTG

Cuts at:      0     693    2225    2448 
   Size:        693    1532     223

  Fragments arranged by size: 

                1532     693     223
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1618    1650    2448 
   Size:       1618      32     798

  Fragments arranged by size: 

                1618     798      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     339    1065    1972    2448 
   Size:         18     321     726     907     476

  Fragments arranged by size: 

                 907     726     476     321      18
 
ApoI r'AATT_y

Cuts at:      0    2415    2448 
   Size:       2415      33
 
AvaI C'yCGr_G

Cuts at:      0     756    2448 
   Size:        756    1692
 
BanI G'GyrC_C

Cuts at:      0     538     841    1870    2108    2126    2448 
   Size:        538     303    1029     238      18     322

  Fragments arranged by size: 

                1029     538     322     303     238      18
 
BanII G_rGCy'C

Cuts at:      0     671     977    1520    2448 
   Size:        671     306     543     928

  Fragments arranged by size: 

                 928     671     543     306
 
BbeI G_GCGC'C

Cuts at:      0    2112    2130    2448 
   Size:       2112      18     318

  Fragments arranged by size: 

                2112     318      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     437    1479    2448 
   Size:        437    1042     969

  Fragments arranged by size: 

                1042     969     437
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     143     390    1620    2448 
   Size:        143     247    1230     828

  Fragments arranged by size: 

                1230     828     247     143
 
BclI T'GATC_A

Cuts at:      0     480     593    2448 
   Size:        480     113    1855

  Fragments arranged by size: 

                1855     480     113
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2044    2448 
   Size:       2044     404
 
BglII A'GATC_T

Cuts at:      0     194    2448 
   Size:        194    2254
 
BlpI GC'TnA_GC

Cuts at:      0    1555    2448 
   Size:       1555     893
 
Bme1580I G_kGCm'C

Cuts at:      0     331     461     541    2448 
   Size:        331     130      80    1907

  Fragments arranged by size: 

                1907     331     130      80
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1287    2126    2448 
   Size:       1287     839     322

  Fragments arranged by size: 

                1287     839     322
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269    2448 
   Size:        269    2179
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     817    2448 
   Size:        817    1631
 
BsaAI yAC'GTr

Cuts at:      0     456    2448 
   Size:        456    1992
 
BsaBI GATnn'nnATC

Cuts at:      0    1607    2448 
   Size:       1607     841
 
BsaHI Gr'CG_yC

Cuts at:      0    1040    1109    1171    1547    2047    2109    2127    2323 
   Size:       1040      69      62     376     500      62      18     196

Cuts at:   2323    2448 
   Size:        125

  Fragments arranged by size: 

                1040     500     376     196     125      69      62      62
                  18
 
BsaWI w'CCGG_w

Cuts at:      0     637    2448 
   Size:        637    1811
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     718    2044    2448 
   Size:        718    1326     404

  Fragments arranged by size: 

                1326     718     404
 
BseYI C'CCAG_C

Cuts at:      0     332     763     857    1104    1444    1448    1914    2448 
   Size:        332     431      94     247     340       4     466     534

  Fragments arranged by size: 

                 534     466     431     340     332     247      94       4
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1360    2448 
   Size:       1360    1088
 
BsiEI CG_ry'CG

Cuts at:      0    2299    2448 
   Size:       2299     149
 
BsiHKAI G_wGCw'C

Cuts at:      0     977    1520    2448 
   Size:        977     543     928

  Fragments arranged by size: 

                 977     928     543
 
BsmI GAATG_Cn'

Cuts at:      0     952    2448 
   Size:        952    1496
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1294    2448 
   Size:       1294    1154
 
Bsp1286I G_dGCh'C

Cuts at:      0     331     461     541     671     977    1520    2448 
   Size:        331     130      80     130     306     543     928

  Fragments arranged by size: 

                 928     543     331     306     130     130      80
 
BspHI T'CATG_A

Cuts at:      0     477    1568    2448 
   Size:        477    1091     880

  Fragments arranged by size: 

                1091     880     477
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1382    2272    2448 
   Size:       1382     890     176

  Fragments arranged by size: 

                1382     890     176
 
BsrBI CCG'CTC

Cuts at:      0     839    2448 
   Size:        839    1609
 
BsrDI GCAATG_nn'

Cuts at:      0    2033    2448 
   Size:       2033     415
 
BsrFI r'CCGG_y

Cuts at:      0     541     637     876    1023    2294    2448 
   Size:        541      96     239     147    1271     154

  Fragments arranged by size: 

                1271     541     239     154     147      96
 
BsrGI T'GTAC_A

Cuts at:      0    1268    2448 
   Size:       1268    1180
 
BssSI C'ACGA_G

Cuts at:      0    2221    2448 
   Size:       2221     227
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     366    2373    2448 
   Size:        366    2007      75

  Fragments arranged by size: 

                2007     366      75
 
BstEII G'GTnAC_C

Cuts at:      0     640    2448 
   Size:        640    1808
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     939    1206    2448 
   Size:        939     267    1242

  Fragments arranged by size: 

                1242     939     267
 
BstYI r'GATC_y

Cuts at:      0     194     349     523    1061    2448 
   Size:        194     155     174     538    1387

  Fragments arranged by size: 

                1387     538     194     174     155
 
Bsu36I CC'TnA_GG

Cuts at:      0    1622    1973    2448 
   Size:       1622     351     475

  Fragments arranged by size: 

                1622     475     351
 
BtgI C'CryG_G

Cuts at:      0     461     516     932    1358    1464    1562    2424    2448 
   Size:        461      55     416     426     106      98     862      24

  Fragments arranged by size: 

                 862     461     426     416     106      98      55      24
            
 
BtsI GCAGTG_nn'

Cuts at:      0     688     869    1422    2448 
   Size:        688     181     553    1026

  Fragments arranged by size: 

                1026     688     553     181
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1739    1774    2448 
   Size:       1739      35     674

  Fragments arranged by size: 

                1739     674      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1039    1984    2448 
   Size:       1039     945     464

  Fragments arranged by size: 

                1039     945     464
 
EaeI y'GGCC_r

Cuts at:      0     878     998    1361    1897    2448 
   Size:        878     120     363     536     551

  Fragments arranged by size: 

                 878     551     536     363     120
 
EarI CTCTTCn'nnn_

Cuts at:      0     820    1525    2062    2174    2448 
   Size:        820     705     537     112     274

  Fragments arranged by size: 

                 820     705     537     274     112
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1323    2448 
   Size:       1323    1125
 
EcoICRI GAG'CTC

Cuts at:      0     975    1518    2448 
   Size:        975     543     930

  Fragments arranged by size: 

                 975     930     543
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269    1150    1250    1506    2376    2448 
   Size:        269     881     100     256     870      72

  Fragments arranged by size: 

                 881     870     269     256     100      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0     168     225     729     767    1476    1953    1969    2448 
   Size:        168      57     504      38     709     477      16     479

  Fragments arranged by size: 

                 709     504     479     477     168      57      38      16
            
 
EcoRV GAT'ATC

Cuts at:      0    2160    2448 
   Size:       2160     288
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     454    2448 
   Size:        454    1994
 
HaeII r_GCGC'y

Cuts at:      0    1562    2086    2112    2130    2448 
   Size:       1562     524      26      18     318

  Fragments arranged by size: 

                1562     524     318      26      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     186     218     334     366     731     763    1840    1872 
   Size:        186      32     116      32     365      32    1077      32

Cuts at:   1872    2173    2175    2205    2207    2215    2247    2448 
   Size:        301       2      30       2       8      32     201

  Fragments arranged by size: 

                1077     365     301     201     186     116      32      32
                  32      32      32      30       8       2       2
 
HincII GTy'rAC

Cuts at:      0      86     144     788    1113    2448 
   Size:         86      58     644     325    1335

  Fragments arranged by size: 

                1335     644     325      86      58
 
HpaI GTT'AAC

Cuts at:      0      86    2448 
   Size:         86    2362
 
KasI G'GCGC_C

Cuts at:      0    2108    2126    2448 
   Size:       2108      18     322

  Fragments arranged by size: 

                2108     322      18
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1223    1751    2448 
   Size:       1223     528     697

  Fragments arranged by size: 

                1223     697     528
 
MscI TGG'CCA

Cuts at:      0    1363    1899    2448 
   Size:       1363     536     549

  Fragments arranged by size: 

                1363     549     536
 
MslI CAynn'nnrTG

Cuts at:      0     341     454     693     937    1469    1498    2225    2338 
   Size:        341     113     239     244     532      29     727     113

Cuts at:   2338    2448 
   Size:        110

  Fragments arranged by size: 

                 727     532     341     244     239     113     113     110
                  29
 
MspA1I CmG'CkG

Cuts at:      0     857    1448    2208    2448 
   Size:        857     591     760     240

  Fragments arranged by size: 

                 857     760     591     240
 
NaeI GCC'GGC

Cuts at:      0     878    2448 
   Size:        878    1570
 
NarI GG'CG_CC

Cuts at:      0    2109    2127    2448 
   Size:       2109      18     321

  Fragments arranged by size: 

                2109     321      18
 
NcoI C'CATG_G

Cuts at:      0     516    1464    2448 
   Size:        516     948     984

  Fragments arranged by size: 

                 984     948     516
 
NgoMIV G'CCGG_C

Cuts at:      0     876    2448 
   Size:        876    1572
 
NspI r_CATG'y

Cuts at:      0     369     924    2448 
   Size:        369     555    1524

  Fragments arranged by size: 

                1524     555     369
 
PasI CC'CwG_GG

Cuts at:      0      12    2448 
   Size:         12    2436
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     339    1347    1895    2448 
   Size:        339    1008     548     553

  Fragments arranged by size: 

                1008     553     548     339
 
PfoI T'CCnGG_A

Cuts at:      0     286     671    2448 
   Size:        286     385    1777

  Fragments arranged by size: 

                1777     385     286
 
PpuMI rG'GwC_Cy

Cuts at:      0     225     729    1476    1953    2448 
   Size:        225     504     747     477     495

  Fragments arranged by size: 

                 747     504     495     477     225
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     949     981    2448 
   Size:        949      32    1467

  Fragments arranged by size: 

                1467     949      32
 
PstI C_TGCA'G

Cuts at:      0    2198    2240    2448 
   Size:       2198      42     208

  Fragments arranged by size: 

                2198     208      42
 
PvuII CAG'CTG

Cuts at:      0     857    1448    2208    2448 
   Size:        857     591     760     240

  Fragments arranged by size: 

                 857     760     591     240
 
SacI G_AGCT'C

Cuts at:      0     977    1520    2448 
   Size:        977     543     928

  Fragments arranged by size: 

                 977     928     543
 
SanDI GG'GwC_CC

Cuts at:      0     225     729    2448 
   Size:        225     504    1719

  Fragments arranged by size: 

                1719     504     225
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1525    2448 
   Size:       1525     923
 
ScaI AGT'ACT

Cuts at:      0     217    2448 
   Size:        217    2231
 
SfcI C'TryA_G

Cuts at:      0    2194    2236    2448 
   Size:       2194      42     212

  Fragments arranged by size: 

                2194     212      42
 
SfoI GGC'GCC

Cuts at:      0    2110    2128    2448 
   Size:       2110      18     320

  Fragments arranged by size: 

                2110     320      18
 
StuI AGG'CCT

Cuts at:      0    1127    2448 
   Size:       1127    1321
 
StyI C'CwwG_G

Cuts at:      0     516    1116    1464    2448 
   Size:        516     600     348     984

  Fragments arranged by size: 

                 984     600     516     348
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1335    1495    2448 
   Size:       1335     160     953

  Fragments arranged by size: 

                1335     953     160
 
TatI w'GTAC_w

Cuts at:      0     215     884    1268    2087    2410    2448 
   Size:        215     669     384     819     323      38

  Fragments arranged by size: 

                 819     669     384     323     215      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1821    1853    2328    2360    2416    2448 
   Size:       1821      32     475      32      56      32

  Fragments arranged by size: 

                1821     475      56      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     792     907    2448 
   Size:        792     115    1541

  Fragments arranged by size: 

                1541     792     115
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     195    2448 
   Size:        195    2253
 
XmnI GAAnn'nnTTC

Cuts at:      0    1778    2448 
   Size:       1778     670
 
ZraI GAC'GTC

Cuts at:      0    1041    1548    2448 
   Size:       1041     507     900

  Fragments arranged by size: 

                1041     900     507
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AgeI     AleI     AloI    AlwNI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
    CspCI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI    HaeII    Hin4I   HincII     HpaI     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI    PpuMI     PsrI     PstI    PvuII     SacI
    SanDI     SapI     ScaI     SfcI     SfoI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AlfI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BfrBI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaXI    BsiWI    BspEI   BssHII    BstBI  BstZ17I    BtgZI     ClaI
     DraI   DraIII     EagI    EcoNI    EcoRI     FseI     FspI    FspAI
  HindIII     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII    SacII     SalI     SbfI    SexAI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     XmaI
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