Restriction map for pF1KSDA1259
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        Asp718I     AspEI       AviII       
AvrII       BbeI        BbrPI       BcgI        BclI        
BfrI        BlnI        BsaAI       BsaHI       BsiEI       
BsiWI       Bsp1407I    BspLU11I    BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       KasI        KpnI        KspI        
MluI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflMI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI         1728  GTCTAC               GT^[AC][GT]AC
AccIII       4628  TCCGGA               T^CCGGA
AflIII       4442  ACGTGT               A^C[GA][CT]GT
AspHI        1066  GAGCAC               G[AT]GC[AT]^C
AvaI         4181  CTCGAG               C^[CT]CG[GA]G
BamHI         198  GGATCC               G^GATCC
BciVI        1226  GTATCC               GTATCC(6/5)
BlpI          843  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      843  GCTCAGC              GC^T[ACGT]AGC
BseAI        4628  TCCGGA               T^CCGGA
BsiHKAI      1066  GAGCAC               G[AT]GC[AT]^C
BsmBI        4218  CGTCTC               CGTCTC(1/5)
BsoBI        4181  CTCGAG               C^[CT]CG[GA]G
BspDI        1279  ATCGAT               AT^CGAT
BspEI        4628  TCCGGA               T^CCGGA
BsrFI        3665  ACCGGC               [GA]^CCGG[CT]
BstAPI       4360  GCAGGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         843  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       3665  ACCGGC               [GA]^CCGG[CT]
ClaI         1279  ATCGAT               AT^CGAT
DraI         1339  TTTAAA               TTT^AAA
HincII       1303  GTTAAC               GT[CT]^[GA]AC
HindII       1303  GTTAAC               GT[CT]^[GA]AC
HpaI         1303  GTTAAC               GTT^AAC
MfeI         1740  CAATTG               C^AATTG
MroI         4628  TCCGGA               T^CCGGA
MunI         1740  CAATTG               C^AATTG
NdeI          578  CATATG               CA^TATG
PaeR7I       4181  CTCGAG               C^TCGAG
SspI          133  AATATT               AAT^ATT
StuI          564  AGGCCT               AGG^CCT
XbaI         3804  TCTAGA               T^CTAGA
XcmI         1886  CCAGCTATCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         4181  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AocI         1242  CCTAAGG              CC^T[ACGT]AGG
             2829  CCTGAGG             
AseI         1609  ATTAAT               AT^TAAT
             2644  ATTAAT              
AsnI         1609  ATTAAT               AT^TAAT
             2644  ATTAAT              
Asp700I       463  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3694  GAACGCATTC          
AspI         1395  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4268  GACGTGGTC           
BbvCI        4166  CCTCAGC              CCTCAGC(-5/-2)
             2229  GCTGAGG             
BglII        3455  AGATCT               A^GATCT
             4076  AGATCT              
BsaBI        2182  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4077  GATCTCCATC          
BsaI         2483  GGTCTC               GGTCTC(1/5)
             2621  GGTCTC              
BsaWI          86  TCCGGT               [AT]^CCGG[AT]
             4628  TCCGGA              
BspMI        1722  ACCTGC               ACCTGC(4/8)
             3651  GCAGGT              
BstXI        3782  CCAAAGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4109  CCATTCCCCTGG        
Bsu36I       1242  CCTAAGG              CC^T[ACGT]AGG
             2829  CCTGAGG             
Eco81I       1242  CCTAAGG              CC^T[ACGT]AGG
             2829  CCTGAGG             
MamI         2182  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4077  GATCTCCATC          
MslI         3504  CATCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4038  CATTAGCGTG          
NcoI         2060  CCATGG               C^CATGG
             3617  CCATGG              
NspI          476  ACATGC               [GA]CATG^[CT]
             4136  ACATGC              
PflFI        1395  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4268  GACGTGGTC           
PshAI         107  GACAAACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              741  GACCAAAGTC          
PshBI        1609  ATTAAT               AT^TAAT
             2644  ATTAAT              
ScaI           68  AGTACT               AGT^ACT
              551  AGTACT              
SexAI        2954  ACCAGGT              A^CC[AT]GGT
             3971  ACCTGGT             
Tsp45I       4133  GTGAC                ^GT[GC]AC
             4274  GTCAC               
Tth111I      1395  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4268  GACGTGGTC           
XmnI          463  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3694  GAACGCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApaI        ApoI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspHI       
BsrDI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
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