Restriction map for pF1KSDA1259
 (Linear) (Six-base) MAPSORT of: KIAA1259.seq  Check: 7004  from: 1  to: 4668



KIAA1259.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1731    4668 
   Size:       1731    2937
 
AccI GT'mk_AC

Cuts at:      0    1729    4668 
   Size:       1729    2939
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     376     731     875    1375    2701    3133    4668 
   Size:         49     327     355     144     500    1326     432    1535

  Fragments arranged by size: 

                1535    1326     500     432     355     327     144      49
            
 
AflIII A'CryG_T

Cuts at:      0    4442    4668 
   Size:       4442     226
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     836    4111    4153    4489    4668 
   Size:        836    3275      42     336     179

  Fragments arranged by size: 

                3275     836     336     179      42
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2329    2361    4553    4585    4668 
   Size:       2329      32    2192      32      83

  Fragments arranged by size: 

                2329    2192      83      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1353    2316    2927    4668 
   Size:       1353     963     611    1741

  Fragments arranged by size: 

                1741    1353     963     611
 
ApaI G_GGCC'C

Cuts at:      0      84    3162    3630    4668 
   Size:         84    3078     468    1038

  Fragments arranged by size: 

                3078    1038     468      84
 
ApoI r'AATT_y

Cuts at:      0     231     333     631    1774    2122    2342    4238    4668 
   Size:        231     102     298    1143     348     220    1896     430

  Fragments arranged by size: 

                1896    1143     430     348     298     231     220     102
            
 
AseI AT'TA_AT

Cuts at:      0    1610    2645    4668 
   Size:       1610    1035    2023

  Fragments arranged by size: 

                2023    1610    1035
 
AvaI C'yCGr_G

Cuts at:      0    4181    4668 
   Size:       4181     487
 
BamHI G'GATC_C

Cuts at:      0     198    4668 
   Size:        198    4470
 
BanI G'GyrC_C

Cuts at:      0      16     605    1745    3682    4645    4668 
   Size:         16     589    1140    1937     963      23

  Fragments arranged by size: 

                1937    1140     963     589      23      16
 
BanII G_rGCy'C

Cuts at:      0      84    2079    2380    3162    3630    4668 
   Size:         84    1995     301     782     468    1038

  Fragments arranged by size: 

                1995    1038     782     468     301      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     434    1262    1849    2360    2547    3790    4668 
   Size:        434     828     587     511     187    1243     878

  Fragments arranged by size: 

                1243     878     828     587     511     434     187
 
BbvCI CC'TCA_GC

Cuts at:      0    2230    4167    4668 
   Size:       2230    1937     501

  Fragments arranged by size: 

                2230    1937     501
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1237    4668 
   Size:       1237    3431
 
BglI GCCn_nnn'nGGC

Cuts at:      0     970    1444    1669    1678    4668 
   Size:        970     474     225       9    2990

  Fragments arranged by size: 

                2990     970     474     225       9
 
BglII A'GATC_T

Cuts at:      0    3455    4076    4668 
   Size:       3455     621     592

  Fragments arranged by size: 

                3455     621     592
 
BlpI GC'TnA_GC

Cuts at:      0     844    4668 
   Size:        844    3824
 
Bme1580I G_kGCm'C

Cuts at:      0      84    1008    3162    3630    4668 
   Size:         84     924    2154     468    1038

  Fragments arranged by size: 

                2154    1038     924     468      84
 
BmgBI CAC'GTC

Cuts at:      0    4270    4668 
   Size:       4270     398
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     183    1475    3550    4668 
   Size:        183    1292    2075    1118

  Fragments arranged by size: 

                2075    1292    1118     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275    1854    2675    3239    3418    3554    4668 
   Size:        275    1579     821     564     179     136    1114

  Fragments arranged by size: 

                1579    1114     821     564     275     179     136
 
Bpu10I CC'TnA_GC

Cuts at:      0    1961    2230    3636    4167    4668 
   Size:       1961     269    1406     531     501

  Fragments arranged by size: 

                1961    1406     531     501     269
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1954    3322    3329    4668 
   Size:       1954    1368       7    1339

  Fragments arranged by size: 

                1954    1368    1339       7
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2489    2627    4668 
   Size:       2489     138    2041

  Fragments arranged by size: 

                2489    2041     138
 
BsaBI GATnn'nnATC

Cuts at:      0    2186    4081    4668 
   Size:       2186    1895     587

  Fragments arranged by size: 

                2186    1895     587
 
BsaWI w'CCGG_w

Cuts at:      0      86    4628    4668 
   Size:         86    4542      40

  Fragments arranged by size: 

                4542      86      40
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1283    1286    1313    1316    2469    2499    4090    4120 
   Size:       1283       3      27       3    1153      30    1591      30

Cuts at:   4120    4147    4177    4179    4209    4668 
   Size:         27      30       2      30     459

  Fragments arranged by size: 

                1591    1283    1153     459      30      30      30      30
                  27      27       3       3       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1307    2247    2976    4155    4668 
   Size:       1307     940     729    1179     513

  Fragments arranged by size: 

                1307    1179     940     729     513
 
BseYI C'CCAG_C

Cuts at:      0    4158    4668 
   Size:       4158     510
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     581    1924    3110    4081    4668 
   Size:         21     560    1343    1186     971     587

  Fragments arranged by size: 

                1343    1186     971     587     560      21
 
BsiHKAI G_wGCw'C

Cuts at:      0    1070    4668 
   Size:       1070    3598
 
BsmI GAATG_Cn'

Cuts at:      0     383     825    1365    1616    3698    4668 
   Size:        383     442     540     251    2082     970

  Fragments arranged by size: 

                2082     970     540     442     383     251
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4224    4668 
   Size:       4224     444
 
Bsp1286I G_dGCh'C

Cuts at:      0      84    1008    1070    2079    2380    3162    3630    4668 
   Size:         84     924      62    1009     301     782     468    1038

  Fragments arranged by size: 

                1038    1009     924     782     468     301      84      62
            
 
BspEI T'CCGG_A

Cuts at:      0    4628    4668 
   Size:       4628      40
 
BspHI T'CATG_A

Cuts at:      0    1193    1218    1806    2414    4668 
   Size:       1193      25     588     608    2254

  Fragments arranged by size: 

                2254    1193     608     588      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1731    3642    4668 
   Size:       1731    1911    1026

  Fragments arranged by size: 

                1911    1731    1026
 
BsrDI GCAATG_nn'

Cuts at:      0     235    1658    3084    4382    4668 
   Size:        235    1423    1426    1298     286

  Fragments arranged by size: 

                1426    1423    1298     286     235
 
BsrFI r'CCGG_y

Cuts at:      0    3665    4668 
   Size:       3665    1003
 
BssSI C'ACGA_G

Cuts at:      0    1001    2567    3044    3527    4668 
   Size:       1001    1566     477     483    1141

  Fragments arranged by size: 

                1566    1141    1001     483     477
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4366    4668 
   Size:       4366     302
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3789    4116    4668 
   Size:       3789     327     552

  Fragments arranged by size: 

                3789     552     327
 
BstYI r'GATC_y

Cuts at:      0     198    1997    3455    4076    4668 
   Size:        198    1799    1458     621     592

  Fragments arranged by size: 

                1799    1458     621     592     198
 
Bsu36I CC'TnA_GG

Cuts at:      0    1243    2830    4668 
   Size:       1243    1587    1838

  Fragments arranged by size: 

                1838    1587    1243
 
BtgI C'CryG_G

Cuts at:      0    2060    3617    4355    4668 
   Size:       2060    1557     738     313

  Fragments arranged by size: 

                2060    1557     738     313
 
BtsI GCAGTG_nn'

Cuts at:      0    2942    3062    3148    3554    4095    4137    4371    4668 
   Size:       2942     120      86     406     541      42     234     297

  Fragments arranged by size: 

                2942     541     406     297     234     120      86      42
            
 
ClaI AT'CG_AT

Cuts at:      0    1280    4668 
   Size:       1280    3388
 
DraI TTT'AAA

Cuts at:      0    1341    4668 
   Size:       1341    3327
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2959    4141    4624    4668 
   Size:       2959    1182     483      44

  Fragments arranged by size: 

                2959    1182     483      44
 
EaeI y'GGCC_r

Cuts at:      0    1586    2729    3035    4668 
   Size:       1586    1143     306    1633

  Fragments arranged by size: 

                1633    1586    1143     306
 
EarI CTCTTCn'nnn_

Cuts at:      0     684    3715    3809    3842    4536    4668 
   Size:        684    3031      94      33     694     132

  Fragments arranged by size: 

                3031     694     684     132      94      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    4449    4668 
   Size:       4449     219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     275     376     731     875    1375    1854    2675 
   Size:         49     226     101     355     144     500     479     821

Cuts at:   2675    2701    3133    3239    3418    3554    4668 
   Size:         26     432     106     179     136    1114

  Fragments arranged by size: 

                1114     821     500     479     432     355     226     179
                 144     136     106     101      49      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1018    2769    3397    3432    4668 
   Size:       1018    1751     628      35    1236

  Fragments arranged by size: 

                1751    1236    1018     628      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0      80      81    1350    1702    2042    3158    3159    3357 
   Size:         80       1    1269     352     340    1116       1     198

Cuts at:   3357    3626    4668 
   Size:        269    1042

  Fragments arranged by size: 

                1269    1116    1042     352     340     269     198      80
                   1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1011    2019    4547    4668 
   Size:       1011    1008    2528     121

  Fragments arranged by size: 

                2528    1011    1008     121
 
HaeII r_GCGC'y

Cuts at:      0    1969    2803    4529    4668 
   Size:       1969     834    1726     139

  Fragments arranged by size: 

                1969    1726     834     139
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    3004    3036    3433    3465    3530    3562    4147    4179 
   Size:       3004      32     397      32      65      32     585      32

Cuts at:   4179    4668 
   Size:        489

  Fragments arranged by size: 

                3004     585     489     397      65      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1305    4668 
   Size:       1305    3363
 
HindIII A'AGCT_T

Cuts at:      0     887     925    1091    4668 
   Size:        887      38     166    3577

  Fragments arranged by size: 

                3577     887     166      38
 
HpaI GTT'AAC

Cuts at:      0    1305    4668 
   Size:       1305    3363
 
MfeI C'AATT_G

Cuts at:      0    1740    4668 
   Size:       1740    2928
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      71    1091    1972    1999    2640    3800    4031    4668 
   Size:         71    1020     881      27     641    1160     231     637

  Fragments arranged by size: 

                1160    1020     881     641     637     231      71      27
            
 
MscI TGG'CCA

Cuts at:      0    1588    2731    3037    4668 
   Size:       1588    1143     306    1631

  Fragments arranged by size: 

                1631    1588    1143     306
 
MslI CAynn'nnrTG

Cuts at:      0    3508    4042    4668 
   Size:       3508     534     626

  Fragments arranged by size: 

                3508     626     534
 
MspA1I CmG'CkG

Cuts at:      0     816    1104    1435    1896    1926    2891    2970    3217 
   Size:        816     288     331     461      30     965      79     247

Cuts at:   3217    3735    4261    4405    4417    4668 
   Size:        518     526     144      12     251

  Fragments arranged by size: 

                 965     816     526     518     461     331     288     251
                 247     144      79      30      12
 
NcoI C'CATG_G

Cuts at:      0    2060    3617    4668 
   Size:       2060    1557    1051

  Fragments arranged by size: 

                2060    1557    1051
 
NdeI CA'TA_TG

Cuts at:      0     579    4668 
   Size:        579    4089
 
NspI r_CATG'y

Cuts at:      0     480    4140    4668 
   Size:        480    3660     528

  Fragments arranged by size: 

                3660     528     480
 
PfoI T'CCnGG_A

Cuts at:      0    1229    4668 
   Size:       1229    3439
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     437     469    4668 
   Size:        437      32    4199

  Fragments arranged by size: 

                4199     437      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1702    2042    3357    4668 
   Size:       1702     340    1315    1311

  Fragments arranged by size: 

                1702    1315    1311     340
 
PshAI GACnn'nnGTC

Cuts at:      0     111     745    4668 
   Size:        111     634    3923

  Fragments arranged by size: 

                3923     634     111
 
PspOMI G'GGCC_C

Cuts at:      0      80    3158    3626    4668 
   Size:         80    3078     468    1042

  Fragments arranged by size: 

                3078    1042     468      80
 
PstI C_TGCA'G

Cuts at:      0    3177    3564    4266    4404    4416    4478    4668 
   Size:       3177     387     702     138      12      62     190

  Fragments arranged by size: 

                3177     702     387     190     138      62      12
 
PvuII CAG'CTG

Cuts at:      0     816    1435    1896    2891    3217    4261    4417    4668 
   Size:        816     619     461     995     326    1044     156     251

  Fragments arranged by size: 

                1044     995     816     619     461     326     251     156
            
 
SanDI GG'GwC_CC

Cuts at:      0    2042    4668 
   Size:       2042    2626
 
ScaI AGT'ACT

Cuts at:      0      70     553    4668 
   Size:         70     483    4115

  Fragments arranged by size: 

                4115     483      70
 
SexAI A'CCwGG_T

Cuts at:      0    2954    3971    4668 
   Size:       2954    1017     697

  Fragments arranged by size: 

                2954    1017     697
 
SfcI C'TryA_G

Cuts at:      0     304     555    2769    3173    3560    4262    4400    4412 
   Size:        304     251    2214     404     387     702     138      12

Cuts at:   4412    4474    4668 
   Size:         62     194

  Fragments arranged by size: 

                2214     702     404     387     304     251     194     138
                  62      12
 
SmlI C'TyrA_G

Cuts at:      0    1969    3337    3344    4181    4668 
   Size:       1969    1368       7     837     487

  Fragments arranged by size: 

                1969    1368     837     487       7
 
SspI AAT'ATT

Cuts at:      0     135    4668 
   Size:        135    4533
 
StuI AGG'CCT

Cuts at:      0     566    4668 
   Size:        566    4102
 
StyI C'CwwG_G

Cuts at:      0    1019    1638    2060    2141    3617    3713    4668 
   Size:       1019     619     422      81    1476      96     955

  Fragments arranged by size: 

                1476    1019     955     619     422      96      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       4    3098    4668 
   Size:          4    3094    1570

  Fragments arranged by size: 

                3094    1570       4
 
TatI w'GTAC_w

Cuts at:      0      68     551    1034    1650    4668 
   Size:         68     483     483     616    3018

  Fragments arranged by size: 

                3018     616     483     483      68
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3188    3220    4090    4122    4668 
   Size:       3188      32     870      32     546

  Fragments arranged by size: 

                3188     870     546      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1398    4271    4668 
   Size:       1398    2873     397

  Fragments arranged by size: 

                2873    1398     397
 
XbaI T'CTAG_A

Cuts at:      0    3804    4668 
   Size:       3804     864
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1893    4668 
   Size:       1893    2775
 
XhoI C'TCGA_G

Cuts at:      0    4181    4668 
   Size:       4181     487
 
XmnI GAAnn'nnTTC

Cuts at:      0     467    3698    4668 
   Size:        467    3231     970

  Fragments arranged by size: 

                3231     970     467
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AlfI     AloI    AlwNI     ApaI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     ClaI     DraI     DrdI     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     HpaI
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NspI
     PfoI     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII    SanDI
     ScaI    SexAI     SfcI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
    ApaLI     AscI    AsiSI    AvrII     BaeI     BbeI     BcgI     BclI
    BfrBI     BmtI     BplI    BsaAI    BsaHI    BsiEI    BsiWI    BsrBI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I    BtgZI    CspCI   DraIII
     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI     KasI
     KpnI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI     XmaI     ZraI
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