Restriction map for pF1KSDA1253
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        Asp700I     Asp718I     AspEI       AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeII       
MluI        MluNI       MroI        MscI        MspI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PpuMI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
SspBI       SspI        StuI        SwaI        XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcsI          385  AAATTT               [GA]^AATT[CT]
Alw44I        361  GTGCAC               G^TGCAC
ApaLI         361  GTGCAC               G^TGCAC
ApoI          385  AAATTT               [GA]^AATT[CT]
AseI         1056  ATTAAT               AT^TAAT
AsnI         1056  ATTAAT               AT^TAAT
AspI          822  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          570  CTCGAG               C^[CT]CG[GA]G
BbsI          789  GTCTTC               GAAGAC(2/6)
BciVI          38  GGATAC               GTATCC(6/5)
BclI          323  TGATCA               T^GATCA
BglII         772  AGATCT               A^GATCT
BpmI         1093  CTGGAG               CTGGAG(16/14)
BpuAI         789  GTCTTC               GAAGAC(2/6)
BsaBI        1293  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         712  CTCCTC               GAGGAG(10/8)
BsmI          418  GCATTC               GAATGC(1/-1)
BsoBI         570  CTCGAG               C^[CT]CG[GA]G
BspHI        1199  TCATGA               T^CATGA
BspMI         472  GCAGGT               ACCTGC(4/8)
BstAPI        394  GCAGCAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         772  AGATCT               [GA]^GATC[CT]
DdeI          874  CTAAG                C^T[ACGT]AG
DraI          382  TTTAAA               TTT^AAA
DsaI           26  CCATGG               C^C[GA][CT]GG
EcoT14I        26  CCATGG               C^C[AT][AT]GG
FbaI          323  TGATCA               T^GATCA
HgaI            8  GCGTC                GACGC(5/10)
HincII        703  GTCAAC               GT[CT]^[GA]AC
HindII        703  GTCAAC               GT[CT]^[GA]AC
MamI         1293  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          398  CAATTG               C^AATTG
MflI          772  AGATCT               [GA]^GATC[CT]
MspA1I       1262  CAGCTG               C[AC]G^C[GT]G
MunI          398  CAATTG               C^AATTG
NcoI           26  CCATGG               C^CATGG
PaeR7I        570  CTCGAG               C^TCGAG
PflFI         822  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         769  CCAAGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1032  GACTC                GAGTC(4/5)
PshAI        1260  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1056  ATTAAT               AT^TAAT
PvuII        1262  CAGCTG               CAG^CTG
RcaI         1199  TCATGA               T^CATGA
SpeI          501  ACTAGT               A^CTAGT
SphI          939  GCATGC               GCATG^C
StyI           26  CCATGG               C^C[AT][AT]GG
TaqI          571  TCGA                 T^CGA
Tsp45I       1144  GTCAC                ^GT[GC]AC
Tth111I       822  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       571  TCGA                 T^CGA
Van91I        769  CCAAGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          570  CTCGAG               C^TCGAG
XhoII         772  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI         1001  GTAC                 GT^AC
             1221  GTAC                
AlwI          346  GATCC                GGATC(4/5)
             1087  GGATC               
AspHI          21  GTGCTC               G[AT]GC[AT]^C
              361  GTGCAC              
BanI          475  GGTGCC               G^G[CT][GA]CC
             1326  GGCACC              
BsaJI          12  CCTGGG               C^C[ACGT][ACGT]GG
               26  CCATGG              
BsiHKAI        21  GTGCTC               G[AT]GC[AT]^C
              361  GTGCAC              
BsiYI         769  CCAAGATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              910  CCAAAGGAAGG         
BslI          769  CCAAGATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              910  CCAAAGGAAGG         
BssSI        1238  CTCGTG               CACGAG(-5/-1)
             1321  CTCGTG              
Cac8I          31  GCGAGC               GC[ACGT]^[ACGT]GC
              939  GCATGC              
EarI         1281  CTCTTC               CTCTTC(1/4)
              333  GAAGAG              
HphI         1272  GGTGA                GGTGA(8/7)
             1113  TCACC               
Ksp632I      1281  CTCTTC               CTCTTC(1/4)
              333  GAAGAG              
MslI           44  CATGTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              524  CACATTCATG          
MwoI          394  GCAGCAATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              878  GCATAATTGGC         
NlaIV         475  GGTGCC               GG[ACGT]^[ACGT]CC
             1326  GGCACC              
NspI          707  ACATGC               [GA]CATG^[CT]
              939  GCATGC              
PstI          356  CTGCAG               CTGCA^G
              392  CTGCAG              
RsaI         1001  GTAC                 GT^AC
             1221  GTAC                
SmlI          570  CTCGAG               C^T[CT][GA]AG
              944  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AccI        AluI        BbvI        BfaI        BmyI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       DpnII       EcoRII      Fnu4HI      FokI        
HinfI       ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIII      Sau3AI      ScrFI       SfaNI       SfcI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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