Restriction map for pF1KSDA1247
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AlwNI       ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AviII       AvrII       BamHI       
BbsI        BciVI       BclI        BfrI        BglII       
BlnI        BpuAI       BsaBI       BseAI       BsiHKAI     
BsiWI       BspDI       BspEI       BspHI       BspLU11I    
BssHII      BstAPI      BstBI       BstYI       ClaI        
CpoI        DraI        Eam1105I    EcoNI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MroI        
MunI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SexAI       
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SwaI        XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          937  GTATAC               GT^[AC][GT]AC
AccII         756  CGCG                 CG^CG
AlwI          779  GGATC                GGATC(4/5)
Aor51HI       845  AGCGCT               AGC^GCT
AspI         1825  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI            9  CCCGAG               C^[CT]CG[GA]G
BanI         2211  GGCGCC               G^G[CT][GA]CC
BbeI         2211  GGCGCC               GGCGC^C
BbvCI        2436  GCTGAGG              CCTCAGC(-5/-2)
BcgI           11  CGAGCCTCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1536  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1536  GCTCAGC              GC^T[ACGT]AGC
BsaI         2562  GAGACC               GGTCTC(1/5)
BsaWI         407  ACCGGA               [AT]^CCGG[AT]
BsiEI        1180  CGGCCG               CG[GA][CT]^CG
BsmI         2376  GAATGC               GAATGC(1/-1)
BsoBI           9  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     1850  TGTACA               T^GTACA
BsrDI        1113  CATTGC               GCAATG(2/0)
BsrGI        1850  TGTACA               T^GTACA
Bst1107I      937  GTATAC               GTA^TAC
BstUI         756  CGCG                 CG^CG
BstZ17I       937  GTATAC               GTA^TAC
CelII        1536  GCTCAGC              GC^T[ACGT]AGC
DraIII        516  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1180  CGGCCG               C^GGCCG
EclXI        1180  CGGCCG               C^GGCCG
Eco47III      845  AGCGCT               AGC^GCT
Eco52I       1180  CGGCCG               C^GGCCG
EcoRI         820  GAATTC               G^AATTC
KasI         2211  GGCGCC               G^GCGCC
MvnI          756  CGCG                 CG^CG
NaeI         1054  GCCGGC               GCC^GGC
NarI         2211  GGCGCC               GG^CGCC
NcoI          878  CCATGG               C^CATGG
NdeI         1001  CATATG               CA^TATG
NgoMIV       1054  GCCGGC               G^CCGGC
PflFI        1825  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         159  CCAGGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1330  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         1860  CCTGCAGG             CCTGCA^GG
SfiI          967  GGCCAGTTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         2211  GGCGCC               GGC^GCC
SphI         2162  GCATGC               GCATG^C
Sse8387I     1860  CCTGCAGG             CCTGCA^GG
SspBI        1850  TGTACA               T^GTACA
SspI          701  AATATT               AAT^ATT
Tth111I      1825  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        159  CCAGGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          820  GAATTC               [GA]^AATT[CT]
             2317  GAATTT              
ApoI          820  GAATTC               [GA]^AATT[CT]
             2317  GAATTT              
BbrPI        2154  CACGTG               CAC^GTG
             2175  CACGTG              
BfaI         1816  CTAG                 C^TAG
             2335  CTAG                
BsmBI         883  GAGACG               CGTCTC(1/5)
              907  GAGACG              
BsrBI        1611  CCGCTC               CCGCTC(-3/-3)
             1177  GAGCGG              
BstEII        955  GGTTACC              G^GT[ACGT]ACC
             1948  GGTCACC             
BstPI         955  GGTTACC              G^GT[ACGT]ACC
             1948  GGTCACC             
DpnII         780  GATC                 ^GATC
             2347  GATC                
EcoO65I       955  GGTTACC              G^GT[ACGT]ACC
             1948  GGTCACC             
EcoT14I       563  CCAAGG               C^C[AT][AT]GG
              878  CCATGG              
MaeI         1816  CTAG                 C^TAG
             2335  CTAG                
MboI          780  GATC                 ^GATC
             2347  GATC                
NdeII         780  GATC                 ^GATC
             2347  GATC                
PmaCI        2154  CACGTG               CAC^GTG
             2175  CACGTG              
PmlI         2154  CACGTG               CAC^GTG
             2175  CACGTG              
PpuMI        1019  GGGTCCC              [GA]G^G[AT]CC[CT]
             1328  AGGACCT             
PvuII        1369  CAGCTG               CAG^CTG
             2368  CAGCTG              
Sau3AI        780  GATC                 ^GATC
             2347  GATC                
ScaI         1484  AGTACT               AGT^ACT
             2339  AGTACT              
StyI          563  CCAAGG               C^C[AT][AT]GG
              878  CCATGG              
XcmI          131  CCAACATCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1607  CCATCCGCTCAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
AocI        ApaI        AvaII       BalI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaAI       BsaHI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstXI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DraII       
DrdI        DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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