Restriction map for pF1KSDA1247
 (Linear) (Six-base) MAPSORT of: KIAA1247.seq  Check: 6376  from: 1  to: 2610



KIAA1247.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     174    2610 
   Size:        174    2436
 
AccI GT'mk_AC

Cuts at:      0     938    2610 
   Size:        938    1672
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1435    1728    1975    2610 
   Size:       1435     293     247     635

  Fragments arranged by size: 

                1435     635     293     247
 
AfeI AGC'GCT

Cuts at:      0     847    2610 
   Size:        847    1763
 
AflIII A'CryG_T

Cuts at:      0     926    2153    2264    2418    2610 
   Size:        926    1227     111     154     192

  Fragments arranged by size: 

                1227     926     192     154     111
 
AleI CACnn'nnGTG

Cuts at:      0     258    1803    2610 
   Size:        258    1545     807

  Fragments arranged by size: 

                1545     807     258
 
ApaI G_GGCC'C

Cuts at:      0       7     800    1042    1438    1710    2610 
   Size:          7     793     242     396     272     900

  Fragments arranged by size: 

                 900     793     396     272     242       7
 
ApoI r'AATT_y

Cuts at:      0     820    2317    2610 
   Size:        820    1497     293

  Fragments arranged by size: 

                1497     820     293
 
AvaI C'yCGr_G

Cuts at:      0       9    2610 
   Size:          9    2601
 
BanI G'GyrC_C

Cuts at:      0    2211    2610 
   Size:       2211     399
 
BanII G_rGCy'C

Cuts at:      0       7     800    1042    1438    1464    1710    2610 
   Size:          7     793     242     396      26     246     900

  Fragments arranged by size: 

                 900     793     396     246     242      26       7
 
BbeI G_GCGC'C

Cuts at:      0    2215    2610 
   Size:       2215     395
 
BbvCI CC'TCA_GC

Cuts at:      0    2437    2610 
   Size:       2437     173
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      34    2610 
   Size:         34    2576
 
BglI GCCn_nnn'nGGC

Cuts at:      0      95     351     974    2610 
   Size:         95     256     623    1636

  Fragments arranged by size: 

                1636     623     256      95
 
BlpI GC'TnA_GC

Cuts at:      0    1537    2610 
   Size:       1537    1073
 
Bme1580I G_kGCm'C

Cuts at:      0       7     800    1042    1438    1481    1710    2610 
   Size:          7     793     242     396      43     229     900

  Fragments arranged by size: 

                 900     793     396     242     229      43       7
 
BmgBI CAC'GTC

Cuts at:      0     617    2610 
   Size:        617    1993
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2236    2590    2610 
   Size:       2236     354      20

  Fragments arranged by size: 

                2236     354      20
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     354    2610 
   Size:        354    2256
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1775    2022    2610 
   Size:        820     955     247     588

  Fragments arranged by size: 

                 955     820     588     247
 
Bpu10I CC'TnA_GC

Cuts at:      0    2437    2610 
   Size:       2437     173
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1144    2008    2116    2610 
   Size:       1144     864     108     494

  Fragments arranged by size: 

                1144     864     494     108
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2556    2610 
   Size:       2556      54
 
BsaAI yAC'GTr

Cuts at:      0     456     929    1032    1650    2156    2177    2267    2421 
   Size:        456     473     103     618     506      21      90     154

Cuts at:   2421    2610 
   Size:        189

  Fragments arranged by size: 

                 618     506     473     456     189     154     103      90
                  21
 
BsaHI Gr'CG_yC

Cuts at:      0    1286    1400    1551    2212    2610 
   Size:       1286     114     151     661     398

  Fragments arranged by size: 

                1286     661     398     151     114
 
BsaWI w'CCGG_w

Cuts at:      0     407    2610 
   Size:        407    2203
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     433     463    2020    2050    2610 
   Size:        433      30    1557      30     560

  Fragments arranged by size: 

                1557     560     433      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     267     330    1309    2454    2610 
   Size:        267      63     979    1145     156

  Fragments arranged by size: 

                1145     979     267     156      63
 
BseYI C'CCAG_C

Cuts at:      0      54     171    1371    1446    1670    2006    2600    2610 
   Size:         54     117    1200      75     224     336     594      10

  Fragments arranged by size: 

                1200     594     336     224     117      75      54      10
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1494    1870    2178    2229    2610 
   Size:       1494     376     308      51     381

  Fragments arranged by size: 

                1494     381     376     308      51
 
BsiEI CG_ry'CG

Cuts at:      0    1183    2610 
   Size:       1183    1427
 
BsmI GAATG_Cn'

Cuts at:      0    2382    2610 
   Size:       2382     228
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     877     901    2610 
   Size:        877      24    1709

  Fragments arranged by size: 

                1709     877      24
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     800    1042    1438    1464    1481    1710    2610 
   Size:          7     793     242     396      26      17     229     900

  Fragments arranged by size: 

                 900     793     396     242     229      26      17       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      89     174    1140    2610 
   Size:         89      85     966    1470

  Fragments arranged by size: 

                1470     966      89      85
 
BsrBI CCG'CTC

Cuts at:      0    1179    1613    2610 
   Size:       1179     434     997

  Fragments arranged by size: 

                1179     997     434
 
BsrDI GCAATG_nn'

Cuts at:      0    1112    2610 
   Size:       1112    1498
 
BsrFI r'CCGG_y

Cuts at:      0     289    1054    1182    2610 
   Size:        289     765     128    1428

  Fragments arranged by size: 

                1428     765     289     128
 
BsrGI T'GTAC_A

Cuts at:      0    1850    2610 
   Size:       1850     760
 
BssSI C'ACGA_G

Cuts at:      0      16     364    1474    1897    2610 
   Size:         16     348    1110     423     713

  Fragments arranged by size: 

                1110     713     423     348      16
 
BstEII G'GTnAC_C

Cuts at:      0     955    1948    2610 
   Size:        955     993     662

  Fragments arranged by size: 

                 993     955     662
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     467     816    1107    1446    1629    2580    2610 
   Size:        467     349     291     339     183     951      30

  Fragments arranged by size: 

                 951     467     349     339     291     183      30
 
BstZ17I GTA'TAC

Cuts at:      0     939    2610 
   Size:        939    1671
 
Bsu36I CC'TnA_GG

Cuts at:      0     683    1712    1933    2610 
   Size:        683    1029     221     677

  Fragments arranged by size: 

                1029     683     677     221
 
BtgI C'CryG_G

Cuts at:      0     675     878     951    1403    2610 
   Size:        675     203      73     452    1207

  Fragments arranged by size: 

                1207     675     452     203      73
 
BtsI GCAGTG_nn'

Cuts at:      0    1393    2387    2467    2610 
   Size:       1393     994      80     143

  Fragments arranged by size: 

                1393     994     143      80
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1716    1751    2610 
   Size:       1716      35     859

  Fragments arranged by size: 

                1716     859      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     521    2610 
   Size:        521    2089
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     861    1018    2401    2610 
   Size:        861     157    1383     209

  Fragments arranged by size: 

                1383     861     209     157
 
EaeI y'GGCC_r

Cuts at:      0     966    1180    1544    1619    1699    1966    2548    2610 
   Size:        966     214     364      75      80     267     582      62

  Fragments arranged by size: 

                 966     582     364     267     214      80      75      62
            
 
EagI C'GGCC_G

Cuts at:      0    1180    2610 
   Size:       1180    1430
 
EarI CTCTTCn'nnn_

Cuts at:      0     718    1570    2314    2554    2610 
   Size:        718     852     744     240      56

  Fragments arranged by size: 

                 852     744     718     240      56
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     115    2610 
   Size:        115    2495
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1435    1728    1775    1975    2022    2610 
   Size:        820     615     293      47     200      47     588

  Fragments arranged by size: 

                 820     615     588     293     200      47      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4     796    1020    1038    1039    1329    1434    1435 
   Size:          4     792     224      18       1     290     105       1

Cuts at:   1435    1706    1707    2061    2230    2610 
   Size:        271       1     354     169     380

  Fragments arranged by size: 

                 792     380     354     290     271     224     169     105
                  18       4       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0     820    2610 
   Size:        820    1790
 
HaeII r_GCGC'y

Cuts at:      0     849    2139    2215    2255    2610 
   Size:        849    1290      76      40     355

  Fragments arranged by size: 

                1290     849     355      76      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1135    1167    2610 
   Size:       1135      32    1443

  Fragments arranged by size: 

                1443    1135      32
 
KasI G'GCGC_C

Cuts at:      0    2211    2610 
   Size:       2211     399
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     462     505     578    1493    2610 
   Size:        462      43      73     915    1117

  Fragments arranged by size: 

                1117     915     462      73      43
 
MscI TGG'CCA

Cuts at:      0    1621    1701    2550    2610 
   Size:       1621      80     849      60

  Fragments arranged by size: 

                1621     849      80      60
 
MslI CAynn'nnrTG

Cuts at:      0     142     184     256     258     814     829     865    1627 
   Size:        142      42      72       2     556      15      36     762

Cuts at:   1627    1792    1803    2610 
   Size:        165      11     807

  Fragments arranged by size: 

                 807     762     556     165     142      72      42      36
                  15      11       2
 
MspA1I CmG'CkG

Cuts at:      0     840    1371    1523    2370    2610 
   Size:        840     531     152     847     240

  Fragments arranged by size: 

                 847     840     531     240     152
 
NaeI GCC'GGC

Cuts at:      0    1056    2610 
   Size:       1056    1554
 
NarI GG'CG_CC

Cuts at:      0    2212    2610 
   Size:       2212     398
 
NcoI C'CATG_G

Cuts at:      0     878    2610 
   Size:        878    1732
 
NdeI CA'TA_TG

Cuts at:      0    1002    2610 
   Size:       1002    1608
 
NgoMIV G'CCGG_C

Cuts at:      0    1054    2610 
   Size:       1054    1556
 
NspI r_CATG'y

Cuts at:      0     834     900    2166    2610 
   Size:        834      66    1266     444

  Fragments arranged by size: 

                1266     834     444      66
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     165    2610 
   Size:        165    2445
 
PfoI T'CCnGG_A

Cuts at:      0    1106    2610 
   Size:       1106    1504
 
PmlI CAC'GTG

Cuts at:      0    2156    2177    2610 
   Size:       2156      21     433

  Fragments arranged by size: 

                2156     433      21
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     325     357    2610 
   Size:        325      32    2253

  Fragments arranged by size: 

                2253     325      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1020    1329    2610 
   Size:       1020     309    1281

  Fragments arranged by size: 

                1281    1020     309
 
PshAI GACnn'nnGTC

Cuts at:      0    1334    2610 
   Size:       1334    1276
 
PsiI TTA'TAA

Cuts at:      0     509    2610 
   Size:        509    2101
 
PspOMI G'GGCC_C

Cuts at:      0       3     796    1038    1434    1706    2610 
   Size:          3     793     242     396     272     904

  Fragments arranged by size: 

                 904     793     396     272     242       3
 
PstI C_TGCA'G

Cuts at:      0    1865    1916    2144    2610 
   Size:       1865      51     228     466

  Fragments arranged by size: 

                1865     466     228      51
 
PvuII CAG'CTG

Cuts at:      0    1371    2370    2610 
   Size:       1371     999     240

  Fragments arranged by size: 

                1371     999     240
 
SanDI GG'GwC_CC

Cuts at:      0    1020    2610 
   Size:       1020    1590
 
SbfI CC_TGCA'GG

Cuts at:      0    1865    2610 
   Size:       1865     745
 
ScaI AGT'ACT

Cuts at:      0    1486    2341    2610 
   Size:       1486     855     269

  Fragments arranged by size: 

                1486     855     269
 
SfcI C'TryA_G

Cuts at:      0    1861    1912    2140    2610 
   Size:       1861      51     228     470

  Fragments arranged by size: 

                1861     470     228      51
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     974    2610 
   Size:        974    1636
 
SfoI GGC'GCC

Cuts at:      0    2213    2610 
   Size:       2213     397
 
SmlI C'TyrA_G

Cuts at:      0    1159    2023    2131    2610 
   Size:       1159     864     108     479

  Fragments arranged by size: 

                1159     864     479     108
 
SphI G_CATG'C

Cuts at:      0    2166    2610 
   Size:       2166     444
 
SspI AAT'ATT

Cuts at:      0     703    2610 
   Size:        703    1907
 
StuI AGG'CCT

Cuts at:      0    1121    1507    1655    1760    1867    2171    2610 
   Size:       1121     386     148     105     107     304     439

  Fragments arranged by size: 

                1121     439     386     304     148     107     105
 
StyI C'CwwG_G

Cuts at:      0     563     878    2610 
   Size:        563     315    1732

  Fragments arranged by size: 

                1732     563     315
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      46     270    1648    2020    2200    2610 
   Size:         46     224    1378     372     180     410

  Fragments arranged by size: 

                1378     410     372     224     180      46
 
TatI w'GTAC_w

Cuts at:      0    1484    1577    1850    2339    2610 
   Size:       1484      93     273     489     271

  Fragments arranged by size: 

                1484     489     273     271      93
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     698     730    2020    2052    2068    2100    2610 
   Size:        698      32    1290      32      16      32     510

  Fragments arranged by size: 

                1290     698     510      32      32      32      16
 
Tth111I GACn'n_nGTC

Cuts at:      0    1828    2610 
   Size:       1828     782
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     138    1614    2610 
   Size:        138    1476     996

  Fragments arranged by size: 

                1476     996     138
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI    BbvCI     BcgI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRI    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI    PvuII
    SanDI     SbfI     ScaI     SfcI     SfiI     SfoI     SmlI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BciVI     BclI    BfrBI    BglII     BmtI    BsaBI  BsiHKAI
    BsiWI    BspEI    BspHI   BssHII   BstAPI    BstBI    BstYI    BtgZI
     ClaI     DraI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI    SexAI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     XbaI
     XhoI     XmaI     XmnI     ZraI
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