Restriction map for pF1KSDA1246
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AvrII       BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        DraI        
DraIII      DrdI        Eco47III    Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        KpnI        KspI        MamI        MluI        
MroI        MseI        NaeI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        SacII       SapI        
SbfI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII        2055  GACGTC               GACGT^C
AccI           52  GTCGAC               GT^[AC][GT]AC
AhdI          509  GACGCATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         509  GACGCATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          243  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1811  TGCGCA               TGC^GCA
BamHI        1922  GGATCC               G^GATCC
BbeI         1741  GGCGCC               GGCGC^C
BbsI         1642  GTCTTC               GAAGAC(2/6)
BbvCI        1143  CCTCAGC              CCTCAGC(-5/-2)
BfaI           25  CTAG                 C^TAG
BlpI          531  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      531  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1642  GTCTTC               GAAGAC(2/6)
BsgI         1166  CTGCAC               GTGCAG(16/14)
BsiEI        1709  CGGCCG               CG[GA][CT]^CG
BsrFI         152  ACCGGC               [GA]^CCGG[CT]
BssSI        1684  CACGAG               CACGAG(-5/-1)
BstAPI        627  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        721  GGTAACC              G^GT[ACGT]ACC
BstPI         721  GGTAACC              G^GT[ACGT]ACC
CelII         531  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        152  ACCGGC               [GA]^CCGG[CT]
CpoI         2078  CGGACCG              CG^G[AT]CCG
EagI         1709  CGGCCG               C^GGCCG
Eam1105I      509  GACGCATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1863  CTCTTC               CTCTTC(1/4)
EclXI        1709  CGGCCG               C^GGCCG
Eco52I       1709  CGGCCG               C^GGCCG
EcoNI         342  CCTTGGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       721  GGTAACC              G^GT[ACGT]ACC
FspI         1811  TGCGCA               TGC^GCA
HaeII        1741  GGCGCC               [GA]GCGC^[CT]
KasI         1741  GGCGCC               G^GCGCC
Ksp632I      1863  CTCTTC               CTCTTC(1/4)
MaeI           25  CTAG                 C^TAG
MaeII        2056  ACGT                 A^CGT
MfeI          738  CAATTG               C^AATTG
MunI          738  CAATTG               C^AATTG
NarI         1741  GGCGCC               GG^CGCC
NheI           24  GCTAGC               G^CTAGC
PflFI         243  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1005  GACCGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        2078  CGGACCG              CG^G[AT]CCG
SacI         1819  GAGCTC               GAGCT^C
SalI           52  GTCGAC               G^TCGAC
ScaI           68  AGTACT               AGT^ACT
SfoI         1741  GGCGCC               GGC^GCC
SphI         2282  GCATGC               GCATG^C
StuI         1421  AGGCCT               AGG^CCT
Tsp509I       739  AATT                 ^AATT
Tth111I       243  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII        1849  CGCG                 CG^CG
             1940  CGCG                
AspHI        1819  GAGCTC               G[AT]GC[AT]^C
             2358  GAGCAC              
BalI         1475  TGGCCA               TGG^CCA
             1628  TGGCCA              
BsiHKAI      1819  GAGCTC               G[AT]GC[AT]^C
             2358  GAGCAC              
BsrDI        1077  CATTGC               GCAATG(2/0)
             1718  GCAATG              
BstUI        1849  CGCG                 CG^CG
             1940  CGCG                
HgaI           55  GACGC                GACGC(5/10)
              509  GACGC               
MluNI        1475  TGGCCA               TGG^CCA
             1628  TGGCCA              
MscI         1475  TGGCCA               TGG^CCA
             1628  TGGCCA              
MvnI         1849  CGCG                 CG^CG
             1940  CGCG                
NcoI          483  CCATGG               C^CATGG
             1109  CCATGG              
NspI          831  GCATGT               [GA]CATG^[CT]
             2282  GCATGC              
PaeR7I        295  CTCGAG               C^TCGAG
              766  CTCGAG              
PflMI         337  CCAAGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1581  CCAGATTCTGG         
PpuMI         103  GGGACCC              [GA]G^G[AT]CC[CT]
              941  GGGACCC             
SexAI        1439  ACCTGGT              A^CC[AT]GGT
             2309  ACCTGGT             
TfiI           84  GAATC                G^A[AT]TC
             1584  GATTC               
Van91I        337  CCAAGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1581  CCAGATTCTGG         
XhoI          295  CTCGAG               C^TCGAG
              766  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       ApaI        AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BglI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
XcmI        XhoII       XmaCI       XmaI        
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