Restriction map for pF1KSDA1246
 (Linear) (Six-base) MAPSORT of: KIAA1246.seq  Check: 6654  from: 1  to: 2367



KIAA1246.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1081    2367 
   Size:       1081    1286
 
AatII G_ACGT'C

Cuts at:      0    2059    2367 
   Size:       2059     308
 
AccI GT'mk_AC

Cuts at:      0      53    2367 
   Size:         53    2314
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1307    2367 
   Size:       1307    1060
 
AhdI GACnn_n'nnGTC

Cuts at:      0     514    2367 
   Size:        514    1853
 
AleI CACnn'nnGTG

Cuts at:      0     924    2367 
   Size:        924    1443
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     349    2305    2367 
   Size:        349    1956      62

  Fragments arranged by size: 

                1956     349      62
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     594     633    1453    1587    2367 
   Size:         87     507      39     820     134     780

  Fragments arranged by size: 

                 820     780     507     134      87      39
 
ApaI G_GGCC'C

Cuts at:      0    1782    1795    1914    2102    2322    2367 
   Size:       1782      13     119     188     220      45

  Fragments arranged by size: 

                1782     220     188     119      45      13
 
AvaI C'yCGr_G

Cuts at:      0     295     766    1340    2063    2111    2321    2367 
   Size:        295     471     574     723      48     210      46

  Fragments arranged by size: 

                 723     574     471     295     210      48      46
 
BamHI G'GATC_C

Cuts at:      0    1922    2367 
   Size:       1922     445
 
BanI G'GyrC_C

Cuts at:      0     571    1024    1063    1594    1741    2367 
   Size:        571     453      39     531     147     626

  Fragments arranged by size: 

                 626     571     531     453     147      39
 
BanII G_rGCy'C

Cuts at:      0    1782    1795    1823    1914    2102    2322    2367 
   Size:       1782      13      28      91     188     220      45

  Fragments arranged by size: 

                1782     220     188      91      45      28      13
 
BbeI G_GCGC'C

Cuts at:      0    1745    2367 
   Size:       1745     622
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1635    2367 
   Size:       1635     732
 
BbvCI CC'TCA_GC

Cuts at:      0    1144    2367 
   Size:       1144    1223
 
BglI GCCn_nnn'nGGC

Cuts at:      0     179    1099    1843    2367 
   Size:        179     920     744     524

  Fragments arranged by size: 

                 920     744     524     179
 
BlpI GC'TnA_GC

Cuts at:      0     532    2367 
   Size:        532    1835
 
Bme1580I G_kGCm'C

Cuts at:      0     115     574    1782    1795    1914    2102    2322    2367 
   Size:        115     459    1208      13     119     188     220      45

  Fragments arranged by size: 

                1208     459     220     188     119     115      45      13
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     109     972    1527    2367 
   Size:        109     863     555     840

  Fragments arranged by size: 

                 863     840     555     109
 
BmtI G_CTAG'C

Cuts at:      0      28    2367 
   Size:         28    2339
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922    1188    1854    1938    2010    2018    2367 
   Size:        922     266     666      84      72       8     349

  Fragments arranged by size: 

                 922     666     349     266      84      72       8
 
Bpu10I CC'TnA_GC

Cuts at:      0    1144    2367 
   Size:       1144    1223
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     796     907     984    1978    2367 
   Size:        796     111      77     994     389

  Fragments arranged by size: 

                 994     796     389     111      77
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1125    2247    2367 
   Size:       1125    1122     120

  Fragments arranged by size: 

                1125    1122     120
 
BsaHI Gr'CG_yC

Cuts at:      0      56    1742    2056    2367 
   Size:         56    1686     314     311

  Fragments arranged by size: 

                1686     314     311      56
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     838     868    2195    2225    2367 
   Size:        838      30    1327      30     142

  Fragments arranged by size: 

                1327     838     142      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     856     859     987    1851    2026    2222    2314    2367 
   Size:        856       3     128     864     175     196      92      53

  Fragments arranged by size: 

                 864     856     196     175     128      92      53       3
            
 
BseYI C'CCAG_C

Cuts at:      0     405     875     946    1407    1693    1745    1902    1951 
   Size:        405     470      71     461     286      52     157      49

Cuts at:   1951    2050    2094    2367 
   Size:         99      44     273

  Fragments arranged by size: 

                 470     461     405     286     273     157      99      71
                  52      49      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1151    2367 
   Size:       1151    1216
 
BsiEI CG_ry'CG

Cuts at:      0    1712    2367 
   Size:       1712     655
 
BsiHKAI G_wGCw'C

Cuts at:      0    1823    2362    2367 
   Size:       1823     539       5

  Fragments arranged by size: 

                1823     539       5
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     574    1782    1795    1823    1914    2102    2322 
   Size:        115     459    1208      13      28      91     188     220

Cuts at:   2322    2362    2367 
   Size:         40       5

  Fragments arranged by size: 

                1208     459     220     188     115      91      40      28
                  13       5
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     383     557     593    1081    2367 
   Size:        383     174      36     488    1286

  Fragments arranged by size: 

                1286     488     383     174      36
 
BsrBI CCG'CTC

Cuts at:      0     308     659     771    2367 
   Size:        308     351     112    1596

  Fragments arranged by size: 

                1596     351     308     112
 
BsrDI GCAATG_nn'

Cuts at:      0    1076    1725    2367 
   Size:       1076     649     642

  Fragments arranged by size: 

                1076     649     642
 
BsrFI r'CCGG_y

Cuts at:      0     152    2367 
   Size:        152    2215
 
BssSI C'ACGA_G

Cuts at:      0    1684    2367 
   Size:       1684     683
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     633    2367 
   Size:        633    1734
 
BstEII G'GTnAC_C

Cuts at:      0     721    2367 
   Size:        721    1646
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1110    1521    1605    1638    1701    1878    2367 
   Size:       1110     411      84      33      63     177     489

  Fragments arranged by size: 

                1110     489     411     177      84      63      33
 
BstYI r'GATC_y

Cuts at:      0     456     606    1922    2367 
   Size:        456     150    1316     445

  Fragments arranged by size: 

                1316     456     445     150
 
BtgI C'CryG_G

Cuts at:      0      47     483     493    1103    1109    2367 
   Size:         47     436      10     610       6    1258

  Fragments arranged by size: 

                1258     610     436      47      10       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     399     546    2367 
   Size:        399     147    1821

  Fragments arranged by size: 

                1821     399     147
 
BtsI GCAGTG_nn'

Cuts at:      0     732    1163    1239    1567    1890    2089    2367 
   Size:        732     431      76     328     323     199     278

  Fragments arranged by size: 

                 732     431     328     323     278     199      76
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     672     707    2367 
   Size:        672      35    1660

  Fragments arranged by size: 

                1660     672      35
 
EaeI y'GGCC_r

Cuts at:      0     914    1106    1475    1508    1628    1709    2367 
   Size:        914     192     369      33     120      81     658

  Fragments arranged by size: 

                 914     658     369     192     120      81      33
 
EagI C'GGCC_G

Cuts at:      0    1709    2367 
   Size:       1709     658
 
EarI CTCTTCn'nnn_

Cuts at:      0    1869    2367 
   Size:       1869     498
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     171    1256    1923    2367 
   Size:        171    1085     667     444

  Fragments arranged by size: 

                1085     667     444     171
 
EcoICRI GAG'CTC

Cuts at:      0    1821    2367 
   Size:       1821     546
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922    1188    1307    1854    1938    2010    2018    2367 
   Size:        922     266     119     547      84      72       8     349

  Fragments arranged by size: 

                 922     547     349     266     119      84      72       8
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     346    2367 
   Size:        346    2021
 
EcoO109I rG'GnC_Cy

Cuts at:      0     104     363     807     942    1689    1778    1779    1791 
   Size:        104     259     444     135     747      89       1      12

Cuts at:   1791    1911    1973    2098    2099    2318    2367 
   Size:        120      62     125       1     219      49

  Fragments arranged by size: 

                 747     444     259     219     135     125     120     104
                  89      62      49      12       1       1
 
FspI TGC'GCA

Cuts at:      0    1813    2367 
   Size:       1813     554
 
HaeII r_GCGC'y

Cuts at:      0    1745    2367 
   Size:       1745     622
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     500     532     598     630    2367 
   Size:        500      32      66      32    1737

  Fragments arranged by size: 

                1737     500      66      32      32
 
HincII GTy'rAC

Cuts at:      0      54     372     519    1434    2277    2367 
   Size:         54     318     147     915     843      90

  Fragments arranged by size: 

                 915     843     318     147      90      54
 
KasI G'GCGC_C

Cuts at:      0    1741    2367 
   Size:       1741     626
 
MfeI C'AATT_G

Cuts at:      0     738    2367 
   Size:        738    1629
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1439    2120    2367 
   Size:       1439     681     247

  Fragments arranged by size: 

                1439     681     247
 
MscI TGG'CCA

Cuts at:      0    1477    1630    2367 
   Size:       1477     153     737

  Fragments arranged by size: 

                1477     737     153
 
MslI CAynn'nnrTG

Cuts at:      0     836     924    1108    1624    1657    2367 
   Size:        836      88     184     516      33     710

  Fragments arranged by size: 

                 836     710     516     184      88      33
 
MspA1I CmG'CkG

Cuts at:      0     405     531    1009    1134    1359    1708    2367 
   Size:        405     126     478     125     225     349     659

  Fragments arranged by size: 

                 659     478     405     349     225     126     125
 
NarI GG'CG_CC

Cuts at:      0    1742    2367 
   Size:       1742     625
 
NcoI C'CATG_G

Cuts at:      0     483    1109    2367 
   Size:        483     626    1258

  Fragments arranged by size: 

                1258     626     483
 
NheI G'CTAG_C

Cuts at:      0      24    2367 
   Size:         24    2343
 
NspI r_CATG'y

Cuts at:      0     835    2286    2367 
   Size:        835    1451      81

  Fragments arranged by size: 

                1451     835      81
 
PasI CC'CwG_GG

Cuts at:      0     802    1878    2367 
   Size:        802    1076     489

  Fragments arranged by size: 

                1076     802     489
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     343    1587    2367 
   Size:        343    1244     780

  Fragments arranged by size: 

                1244     780     343
 
PfoI T'CCnGG_A

Cuts at:      0     250    1823    2026    2367 
   Size:        250    1573     203     341

  Fragments arranged by size: 

                1573     341     250     203
 
PpuMI rG'GwC_Cy

Cuts at:      0     104     942    2367 
   Size:        104     838    1425

  Fragments arranged by size: 

                1425     838     104
 
PshAI GACnn'nnGTC

Cuts at:      0    1009    2367 
   Size:       1009    1358
 
PspOMI G'GGCC_C

Cuts at:      0    1778    1791    1910    2098    2318    2367 
   Size:       1778      13     119     188     220      49

  Fragments arranged by size: 

                1778     220     188     119      49      13
 
PspXI vC'TCGA_Gb

Cuts at:      0     766    2367 
   Size:        766    1601
 
PstI C_TGCA'G

Cuts at:      0     380     590     629    1364    2367 
   Size:        380     210      39     735    1003

  Fragments arranged by size: 

                1003     735     380     210      39
 
PvuII CAG'CTG

Cuts at:      0     405     531    1134    1359    2367 
   Size:        405     126     603     225    1008

  Fragments arranged by size: 

                1008     603     405     225     126
 
RsrII CG'GwC_CG

Cuts at:      0    2079    2367 
   Size:       2079     288
 
SacI G_AGCT'C

Cuts at:      0    1823    2367 
   Size:       1823     544
 
SalI G'TCGA_C

Cuts at:      0      52    2367 
   Size:         52    2315
 
SanDI GG'GwC_CC

Cuts at:      0     104     942    2367 
   Size:        104     838    1425

  Fragments arranged by size: 

                1425     838     104
 
ScaI AGT'ACT

Cuts at:      0      70    2367 
   Size:         70    2297
 
SexAI A'CCwGG_T

Cuts at:      0    1439    2309    2367 
   Size:       1439     870      58

  Fragments arranged by size: 

                1439     870      58
 
SfcI C'TryA_G

Cuts at:      0     376     586     625    1360    2223    2367 
   Size:        376     210      39     735     863     144

  Fragments arranged by size: 

                 863     735     376     210     144      39
 
SfoI GGC'GCC

Cuts at:      0    1743    2367 
   Size:       1743     624
 
SmaI CCC'GGG

Cuts at:      0    1342    2065    2113    2323    2367 
   Size:       1342     723      48     210      44

  Fragments arranged by size: 

                1342     723     210      48      44
 
SmlI C'TyrA_G

Cuts at:      0     295     766     811     922     999    1993    2367 
   Size:        295     471      45     111      77     994     374

  Fragments arranged by size: 

                 994     471     374     295     111      77      45
 
SphI G_CATG'C

Cuts at:      0    2286    2367 
   Size:       2286      81
 
SrfI GCCC'GGGC

Cuts at:      0    2113    2367 
   Size:       2113     254
 
StuI AGG'CCT

Cuts at:      0    1423    2367 
   Size:       1423     944
 
StyI C'CwwG_G

Cuts at:      0     119     342     483    1109    1544    2367 
   Size:        119     223     141     626     435     823

  Fragments arranged by size: 

                 823     626     435     223     141     119
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     889    2096    2337    2367 
   Size:        889    1207     241      30

  Fragments arranged by size: 

                1207     889     241      30
 
TatI w'GTAC_w

Cuts at:      0      68    1364    1724    2367 
   Size:         68    1296     360     643

  Fragments arranged by size: 

                1296     643     360      68
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     838     870    2195    2227    2367 
   Size:        838      32    1325      32     140

  Fragments arranged by size: 

                1325     838     140      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     246    2367 
   Size:        246    2121
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1110    1305    1518    2367 
   Size:       1110     195     213     849

  Fragments arranged by size: 

                1110     849     213     195
 
XhoI C'TCGA_G

Cuts at:      0     295     766    2367 
   Size:        295     471    1601

  Fragments arranged by size: 

                1601     471     295
 
XmaI C'CCGG_G

Cuts at:      0    1340    2063    2111    2321    2367 
   Size:       1340     723      48     210      46

  Fragments arranged by size: 

                1340     723     210      48      46
 
ZraI GAC'GTC

Cuts at:      0    2057    2367 
   Size:       2057     310
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AhdI     AleI     AlfI    AlwNI
     ApaI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BglI     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I   HincII     KasI     MfeI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NheI     NspI     PasI    PflMI     PfoI    PpuMI
    PshAI   PspOMI    PspXI     PstI    PvuII    RsrII     SacI     SalI
    SanDI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AloI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI
     BclI    BfrBI    BglII    BmgBI     BplI    BsaAI    BsaBI    BsaWI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI   BssHII    BstBI
  BstZ17I   Bsu36I     ClaI     DraI   DraIII     DrdI    EcoRI    EcoRV
     FalI     FseI    FspAI  HindIII     HpaI     KpnI     MluI     NaeI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI     PsiI     PsrI     PvuI    SacII     SapI     SbfI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI     XbaI
     XmnI
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