Restriction map for pF1KSDA1242
 (Linear) (Six-base) MAPSORT of: KIAA1242.seq  Check: 4099  from: 1  to: 1479



KIAA1242.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     628    1479 
   Size:        628     851
 
AfeI AGC'GCT

Cuts at:      0     406    1479 
   Size:        406    1073
 
AflIII A'CryG_T

Cuts at:      0      66     816    1479 
   Size:         66     750     663

  Fragments arranged by size: 

                 750     663      66
 
AhdI GACnn_n'nnGTC

Cuts at:      0     585    1479 
   Size:        585     894
 
AleI CACnn'nnGTG

Cuts at:      0     622    1179    1479 
   Size:        622     557     300

  Fragments arranged by size: 

                 622     557     300
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     895    1479 
   Size:        895     584
 
AlwNI CAG_nnn'CTG

Cuts at:      0     477    1479 
   Size:        477    1002
 
AvaI C'yCGr_G

Cuts at:      0     544     987    1026    1116    1479 
   Size:        544     443      39      90     363

  Fragments arranged by size: 

                 544     443     363      90      39
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1125    1158    1479 
   Size:       1125      33     321

  Fragments arranged by size: 

                1125     321      33
 
BamHI G'GATC_C

Cuts at:      0    1472    1479 
   Size:       1472       7
 
BanI G'GyrC_C

Cuts at:      0      20     301     616    1479 
   Size:         20     281     315     863

  Fragments arranged by size: 

                 863     315     281      20
 
BanII G_rGCy'C

Cuts at:      0     956    1039    1198    1479 
   Size:        956      83     159     281

  Fragments arranged by size: 

                 956     281     159      83
 
BbeI G_GCGC'C

Cuts at:      0      24    1479 
   Size:         24    1455
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1153    1479 
   Size:       1153     326
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1075    1479 
   Size:       1075     404
 
BglII A'GATC_T

Cuts at:      0     509     761    1479 
   Size:        509     252     718

  Fragments arranged by size: 

                 718     509     252
 
BlpI GC'TnA_GC

Cuts at:      0     601     842    1479 
   Size:        601     241     637

  Fragments arranged by size: 

                 637     601     241
 
Bme1580I G_kGCm'C

Cuts at:      0     742    1325    1479 
   Size:        742     583     154

  Fragments arranged by size: 

                 742     583     154
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     103     206    1479 
   Size:        103     103    1273

  Fragments arranged by size: 

                1273     103     103
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135     189     393    1406    1479 
   Size:        135      54     204    1013      73

  Fragments arranged by size: 

                1013     204     135      73      54
 
Bpu10I CC'TnA_GC

Cuts at:      0     394    1033    1479 
   Size:        394     639     446

  Fragments arranged by size: 

                 639     446     394
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     940    1479 
   Size:        268     672     539

  Fragments arranged by size: 

                 672     539     268
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     128    1387    1479 
   Size:        128    1259      92

  Fragments arranged by size: 

                1259     128      92
 
BsaAI yAC'GTr

Cuts at:      0     386    1479 
   Size:        386    1093
 
BsaBI GATnn'nnATC

Cuts at:      0      40    1479 
   Size:         40    1439
 
BsaHI Gr'CG_yC

Cuts at:      0      21      94     236    1479 
   Size:         21      73     142    1243

  Fragments arranged by size: 

                1243     142      73      21
 
BsaWI w'CCGG_w

Cuts at:      0     221     482    1479 
   Size:        221     261     997

  Fragments arranged by size: 

                 997     261     221
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     627     657    1180    1210    1432    1462    1479 
   Size:        627      30     523      30     222      30      17

  Fragments arranged by size: 

                 627     523     222      30      30      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     970    1479 
   Size:        970     509
 
BseYI C'CCAG_C

Cuts at:      0     121     297    1479 
   Size:        121     176    1182

  Fragments arranged by size: 

                1182     176     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     778    1063    1479 
   Size:        340     438     285     416

  Fragments arranged by size: 

                 438     416     340     285
 
BsiEI CG_ry'CG

Cuts at:      0     870    1070    1479 
   Size:        870     200     409

  Fragments arranged by size: 

                 870     409     200
 
BsiHKAI G_wGCw'C

Cuts at:      0     602    1061    1249    1457    1479 
   Size:        602     459     188     208      22

  Fragments arranged by size: 

                 602     459     208     188      22
 
BsmI GAATG_Cn'

Cuts at:      0     843    1479 
   Size:        843     636
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1382    1479 
   Size:       1382      97
 
Bsp1286I G_dGCh'C

Cuts at:      0     602     742     956    1039    1061    1198    1249    1325 
   Size:        602     140     214      83      22     137      51      76

Cuts at:   1325    1457    1479 
   Size:        132      22

  Fragments arranged by size: 

                 602     214     140     137     132      83      76      51
                  22      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     628    1479 
   Size:        628     851
 
BsrBI CCG'CTC

Cuts at:      0      18     717     918    1479 
   Size:         18     699     201     561

  Fragments arranged by size: 

                 699     561     201      18
 
BsrDI GCAATG_nn'

Cuts at:      0    1355    1479 
   Size:       1355     124
 
BsrFI r'CCGG_y

Cuts at:      0     304     935    1479 
   Size:        304     631     544

  Fragments arranged by size: 

                 631     544     304
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1107    1479 
   Size:       1107     372
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1239    1479 
   Size:       1239     240
 
BstYI r'GATC_y

Cuts at:      0     509     518     761    1271    1472    1479 
   Size:        509       9     243     510     201       7

  Fragments arranged by size: 

                 510     509     243     201       9       7
 
Bsu36I CC'TnA_GG

Cuts at:      0    1399    1479 
   Size:       1399      80
 
BtgI C'CryG_G

Cuts at:      0     701    1479 
   Size:        701     778
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1065    1479 
   Size:       1065     414
 
BtsI GCAGTG_nn'

Cuts at:      0     904    1174    1186    1479 
   Size:        904     270      12     293

  Fragments arranged by size: 

                 904     293     270      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1440    1479 
   Size:       1440      39
 
EaeI y'GGCC_r

Cuts at:      0    1067    1307    1479 
   Size:       1067     240     172

  Fragments arranged by size: 

                1067     240     172
 
EagI C'GGCC_G

Cuts at:      0    1067    1479 
   Size:       1067     412
 
EarI CTCTTCn'nnn_

Cuts at:      0    1468    1479 
   Size:       1468      11
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      99    1479 
   Size:         99    1380
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135     189     393    1406    1479 
   Size:        135      54     204    1013      73

  Fragments arranged by size: 

                1013     204     135      73      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1300    1479 
   Size:       1300     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0     324    1479 
   Size:        324    1155
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     540    1479 
   Size:        540     939
 
HaeII r_GCGC'y

Cuts at:      0      24     408     829    1479 
   Size:         24     384     421     650

  Fragments arranged by size: 

                 650     421     384      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258     571     603     835     867    1180    1212 
   Size:        226      32     313      32     232      32     313      32

Cuts at:   1212    1432    1464    1479 
   Size:        220      32      15

  Fragments arranged by size: 

                 313     313     232     226     220      32      32      32
                  32      32      15
 
KasI G'GCGC_C

Cuts at:      0      20    1479 
   Size:         20    1459
 
MluI A'CGCG_T

Cuts at:      0      66     816    1479 
   Size:         66     750     663

  Fragments arranged by size: 

                 750     663      66
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     779     893    1479 
   Size:        779     114     586

  Fragments arranged by size: 

                 779     586     114
 
MscI TGG'CCA

Cuts at:      0    1309    1479 
   Size:       1309     170
 
MslI CAynn'nnrTG

Cuts at:      0      76     622    1179    1479 
   Size:         76     546     557     300

  Fragments arranged by size: 

                 557     546     300      76
 
MspA1I CmG'CkG

Cuts at:      0     849    1132    1139    1479 
   Size:        849     283       7     340

  Fragments arranged by size: 

                 849     340     283       7
 
NaeI GCC'GGC

Cuts at:      0     306    1479 
   Size:        306    1173
 
NarI GG'CG_CC

Cuts at:      0      21    1479 
   Size:         21    1458
 
NgoMIV G'CCGG_C

Cuts at:      0     304    1479 
   Size:        304    1175
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1085    1479 
   Size:       1085     394
 
PfoI T'CCnGG_A

Cuts at:      0     856    1479 
   Size:        856     623
 
PpuMI rG'GwC_Cy

Cuts at:      0     324    1479 
   Size:        324    1155
 
PspXI vC'TCGA_Gb

Cuts at:      0    1026    1479 
   Size:       1026     453
 
PstI C_TGCA'G

Cuts at:      0     353     554     884     911     929    1181    1479 
   Size:        353     201     330      27      18     252     298

  Fragments arranged by size: 

                 353     330     298     252     201      27      18
 
PvuII CAG'CTG

Cuts at:      0     849    1132    1479 
   Size:        849     283     347

  Fragments arranged by size: 

                 849     347     283
 
SexAI A'CCwGG_T

Cuts at:      0     725    1479 
   Size:        725     754
 
SfcI C'TryA_G

Cuts at:      0     349     550     880     907     925    1177    1479 
   Size:        349     201     330      27      18     252     302

  Fragments arranged by size: 

                 349     330     302     252     201      27      18
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1075    1479 
   Size:       1075     404
 
SfoI GGC'GCC

Cuts at:      0      22    1479 
   Size:         22    1457
 
SmlI C'TyrA_G

Cuts at:      0     283     955    1026    1479 
   Size:        283     672      71     453

  Fragments arranged by size: 

                 672     453     283      71
 
StyI C'CwwG_G

Cuts at:      0     230    1479 
   Size:        230    1249
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     289    1479 
   Size:        289    1190
 
TatI w'GTAC_w

Cuts at:      0    1049    1479 
   Size:       1049     430
 
XhoI C'TCGA_G

Cuts at:      0    1026    1479 
   Size:       1026     453
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AhdI     AleI     AlfI    AlwNI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI    BciVI     BglI    BglII
     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I     KasI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV    PflMI     PfoI    PpuMI    PspXI     PstI
    PvuII    SexAI     SfcI     SfiI     SfoI     SmlI     StyI    TaqII
     TatI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BbsI
    BbvCI     BcgI     BclI    BfrBI    BmgBI     BmtI     BplI    BsiWI
    BspEI    BspHI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
     ClaI    CspCI     DraI   DraIII  EcoICRI    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI
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