Restriction map for pF1KSDA1218
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BsaAI       
BsaBI       BsaI        BsiEI       BsiWI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
KasI        KpnI        MaeII       MamI        MfeI        
MluI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PflFI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          293  GTAGAC               GT^[AC][GT]AC
AccIII         30  TCCGGA               T^CCGGA
AcyI           70  GACGCC               G[GA]^CG[CT]C
Alw44I       2014  GTGCAC               G^TGCAC
AocI         2063  CCTCAGG              CC^T[ACGT]AGG
ApaLI        2014  GTGCAC               G^TGCAC
Asp700I       994  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          180  TGGCCA               TGG^CCA
BamHI         816  GGATCC               G^GATCC
BbvCI        1686  CCTCAGC              CCTCAGC(-5/-2)
BglII         683  AGATCT               A^GATCT
BlpI          870  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      870  GCTAAGC              GC^T[ACGT]AGC
BsaHI          70  GACGCC               G[GA]^CG[CT]C
BseAI          30  TCCGGA               T^CCGGA
BsgI          219  GTGCAG               GTGCAG(16/14)
BsmI         1956  GAATGC               GAATGC(1/-1)
BspEI          30  TCCGGA               T^CCGGA
BssSI         987  CACGAG               CACGAG(-5/-1)
BstEII       1204  GGTGACC              G^GT[ACGT]ACC
BstPI        1204  GGTGACC              G^GT[ACGT]ACC
BstXI        1298  CCAGACCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2063  CCTCAGG              CC^T[ACGT]AGG
CelII         870  GCTAAGC              GC^T[ACGT]AGC
DraIII       2042  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       2063  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1204  GGTGACC              G^GT[ACGT]ACC
EcoT14I      1397  CCATGG               C^C[AT][AT]GG
HaeII        1639  AGCGCC               [GA]GCGC^[CT]
HgaI           70  GACGC                GACGC(5/10)
Hin1I          70  GACGCC               G[GA]^CG[CT]C
HincII       2365  GTTAAC               GT[CT]^[GA]AC
HindII       2365  GTTAAC               GT[CT]^[GA]AC
HpaI         2365  GTTAAC               GTT^AAC
KspI         2093  CCGCGG               CCGC^GG
MluNI         180  TGGCCA               TGG^CCA
MroI           30  TCCGGA               T^CCGGA
MscI          180  TGGCCA               TGG^CCA
NaeI         1017  GCCGGC               GCC^GGC
NcoI         1397  CCATGG               C^CATGG
NgoMIV       1017  GCCGGC               G^CCGGC
PacI          634  TTAATTAA             TTAAT^TAA
PflMI        1192  CCACCGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2180  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2137  GACTCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          584  CTGCAG               CTGCA^G
SacI         1050  GAGCTC               GAGCT^C
SacII        2093  CCGCGG               CCGC^GG
SexAI         233  ACCAGGT              A^CC[AT]GGT
SgrAI        2348  CACCGGTG             C[GA]^CCGG[CT]G
SmaI          944  CCCGGG               CCC^GGG
SpeI          342  ACTAGT               A^CTAGT
StyI         1397  CCATGG               C^C[AT][AT]GG
Van91I       1192  CCACCGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         944  CCCGGG               C^CCGGG
XmaI          944  CCCGGG               C^CCGGG
XmnI          994  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII        2094  CGCG                 CG^CG
             2118  CGCG                
AgeI         1194  ACCGGT               A^CCGGT
             2349  ACCGGT              
AspHI        1050  GAGCTC               G[AT]GC[AT]^C
             2014  GTGCAC              
BsiHKAI      1050  GAGCTC               G[AT]GC[AT]^C
             2014  GTGCAC              
BsmBI         618  CGTCTC               CGTCTC(1/5)
             1916  CGTCTC              
BspMI         853  ACCTGC               ACCTGC(4/8)
             2385  GCAGGT              
BstUI        2094  CGCG                 CG^CG
             2118  CGCG                
DsaI         1397  CCATGG               C^C[GA][CT]GG
             2093  CCGCGG              
EcoNI         466  CCTGCGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              534  CCTAGTGAAGG         
EcoRI         701  GAATTC               G^AATTC
             1416  GAATTC              
FauI         2092  CCCGC                CCCGC(4/6)
             2331  CCCGC               
MspA1I        372  CAGCGG               C[AC]G^C[GT]G
             2093  CCGCGG              
MvnI         2094  CGCG                 CG^CG
             2118  CGCG                
PaeR7I       1563  CTCGAG               C^TCGAG
             2262  CTCGAG              
PinAI        1194  ACCGGT               A^CCGGT
             2349  ACCGGT              
SfcI          506  CTGTAG               C^T[GA][CT]AG
              584  CTGCAG              
SmlI         1563  CTCGAG               C^T[CT][GA]AG
             2262  CTCGAG              
XhoI         1563  CTCGAG               C^TCGAG
             2262  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BsaWI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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