Restriction map for pF1KSDA1218
 (Linear) (Six-base) MAPSORT of: KIAA1218.seq  Check: 6300  from: 1  to: 2499



KIAA1218.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     862    2499 
   Size:        862    1637
 
AccI GT'mk_AC

Cuts at:      0     294    2499 
   Size:        294    2205
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1028    1532    2499 
   Size:       1028     504     967

  Fragments arranged by size: 

                1028     967     504
 
AgeI A'CCGG_T

Cuts at:      0    1194    2349    2499 
   Size:       1194    1155     150

  Fragments arranged by size: 

                1194    1155     150
 
AleI CACnn'nnGTG

Cuts at:      0    1425    2499 
   Size:       1425    1074
 
AlwNI CAG_nnn'CTG

Cuts at:      0     227     505     592    2499 
   Size:        227     278      87    1907

  Fragments arranged by size: 

                1907     278     227      87
 
ApaLI G'TGCA_C

Cuts at:      0    2014    2499 
   Size:       2014     485
 
ApoI r'AATT_y

Cuts at:      0     524     701     784    1416    2499 
   Size:        524     177      83     632    1083

  Fragments arranged by size: 

                1083     632     524     177      83
 
AvaI C'yCGr_G

Cuts at:      0     820     944    1563    1635    2262    2499 
   Size:        820     124     619      72     627     237

  Fragments arranged by size: 

                 820     627     619     237     124      72
 
BamHI G'GATC_C

Cuts at:      0     816    2499 
   Size:        816    1683
 
BanI G'GyrC_C

Cuts at:      0     550     739    2352    2499 
   Size:        550     189    1613     147

  Fragments arranged by size: 

                1613     550     189     147
 
BanII G_rGCy'C

Cuts at:      0    1054    1184    2041    2063    2499 
   Size:       1054     130     857      22     436

  Fragments arranged by size: 

                1054     857     436     130      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     282    1034    1853    2204    2499 
   Size:        282     752     819     351     295

  Fragments arranged by size: 

                 819     752     351     295     282
 
BbvCI CC'TCA_GC

Cuts at:      0    1687    2499 
   Size:       1687     812
 
BglII A'GATC_T

Cuts at:      0     683    2499 
   Size:        683    1816
 
BlpI GC'TnA_GC

Cuts at:      0     871    2499 
   Size:        871    1628
 
Bme1580I G_kGCm'C

Cuts at:      0      26    2018    2159    2357    2499 
   Size:         26    1992     141     198     142

  Fragments arranged by size: 

                1992     198     142     141      26
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      20    1523    2499 
   Size:         20    1503     976

  Fragments arranged by size: 

                1503     976      20
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1225    2499 
   Size:       1225    1274
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     302     423    1160    2077    2499 
   Size:        293       9     121     737     917     422

  Fragments arranged by size: 

                 917     737     422     293     121       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    1687    2499 
   Size:       1687     812
 
BsaHI Gr'CG_yC

Cuts at:      0      71    2499 
   Size:         71    2428
 
BsaWI w'CCGG_w

Cuts at:      0      30    1194    2349    2499 
   Size:         30    1164    1155     150

  Fragments arranged by size: 

                1164    1155     150      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2359    2389    2499 
   Size:       2359      30     110

  Fragments arranged by size: 

                2359     110      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     588     907    1863    1866    1869    1872    1875    1878 
   Size:        588     319     956       3       3       3       3       3

Cuts at:   1878    1881    2367    2499 
   Size:          3     486     132

  Fragments arranged by size: 

                 956     588     486     319     132       3       3       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     189    2335    2499 
   Size:        189    2146     164

  Fragments arranged by size: 

                2146     189     164
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240    2499 
   Size:        240    2259
 
BsiHKAI G_wGCw'C

Cuts at:      0    1054    2018    2499 
   Size:       1054     964     481

  Fragments arranged by size: 

                1054     964     481
 
BsmI GAATG_Cn'

Cuts at:      0    1962    2499 
   Size:       1962     537
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     624    1922    2499 
   Size:        624    1298     577

  Fragments arranged by size: 

                1298     624     577
 
Bsp1286I G_dGCh'C

Cuts at:      0      26    1054    1184    2018    2041    2063    2159    2357 
   Size:         26    1028     130     834      23      22      96     198

Cuts at:   2357    2499 
   Size:        142

  Fragments arranged by size: 

                1028     834     198     142     130      96      26      23
                  22
 
BspEI T'CCGG_A

Cuts at:      0      30    2499 
   Size:         30    2469
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     862    2376    2499 
   Size:        862    1514     123

  Fragments arranged by size: 

                1514     862     123
 
BsrFI r'CCGG_y

Cuts at:      0    1017    1194    2349    2499 
   Size:       1017     177    1155     150

  Fragments arranged by size: 

                1155    1017     177     150
 
BssSI C'ACGA_G

Cuts at:      0     987    2499 
   Size:        987    1512
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     550     592    1081    2499 
   Size:        550      42     489    1418

  Fragments arranged by size: 

                1418     550     489      42
 
BstEII G'GTnAC_C

Cuts at:      0    1204    2499 
   Size:       1204    1295
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1305    2499 
   Size:       1305    1194
 
BstYI r'GATC_y

Cuts at:      0     683     816     844    1436    2499 
   Size:        683     133      28     592    1063

  Fragments arranged by size: 

                1063     683     592     133      28
 
Bsu36I CC'TnA_GG

Cuts at:      0    2064    2499 
   Size:       2064     435
 
BtgI C'CryG_G

Cuts at:      0    1397    2093    2499 
   Size:       1397     696     406

  Fragments arranged by size: 

                1397     696     406
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1610    2499 
   Size:       1610     889
 
BtsI GCAGTG_nn'

Cuts at:      0      84     228     264    2139    2499 
   Size:         84     144      36    1875     360

  Fragments arranged by size: 

                1875     360     144      84      36
 
DraIII CAC_nnn'GTG

Cuts at:      0    2047    2499 
   Size:       2047     452
 
EaeI y'GGCC_r

Cuts at:      0     180     375    1167    1676    2499 
   Size:        180     195     792     509     823

  Fragments arranged by size: 

                 823     792     509     195     180
 
EarI CTCTTCn'nnn_

Cuts at:      0     104    1581    1899    2498    2499 
   Size:        104    1477     318     599       1

  Fragments arranged by size: 

                1477     599     318     104       1
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1710    2499 
   Size:       1710     789
 
EcoICRI GAG'CTC

Cuts at:      0    1052    2499 
   Size:       1052    1447
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     302     423    1028    1160    1532    2077    2499 
   Size:        293       9     121     605     132     372     545     422

  Fragments arranged by size: 

                 605     545     422     372     293     132     121       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     470     538    2499 
   Size:        470      68    1961

  Fragments arranged by size: 

                1961     470      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1713    1930    2181    2499 
   Size:       1713     217     251     318

  Fragments arranged by size: 

                1713     318     251     217
 
EcoRI G'AATT_C

Cuts at:      0     701    1416    2499 
   Size:        701     715    1083

  Fragments arranged by size: 

                1083     715     701
 
HaeII r_GCGC'y

Cuts at:      0    1643    2499 
   Size:       1643     856
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29    2475    2499 
   Size:         29    2446      24

  Fragments arranged by size: 

                2446      29      24
 
HincII GTy'rAC

Cuts at:      0    2367    2499 
   Size:       2367     132
 
HpaI GTT'AAC

Cuts at:      0    2367    2499 
   Size:       2367     132
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     116    1179    2499 
   Size:        116    1063    1320

  Fragments arranged by size: 

                1320    1063     116
 
MscI TGG'CCA

Cuts at:      0     182    2499 
   Size:        182    2317
 
MslI CAynn'nnrTG

Cuts at:      0     734    1425    2296    2499 
   Size:        734     691     871     203

  Fragments arranged by size: 

                 871     734     691     203
 
MspA1I CmG'CkG

Cuts at:      0     374    2095    2499 
   Size:        374    1721     404

  Fragments arranged by size: 

                1721     404     374
 
NaeI GCC'GGC

Cuts at:      0    1019    2499 
   Size:       1019    1480
 
NcoI C'CATG_G

Cuts at:      0    1397    2499 
   Size:       1397    1102
 
NgoMIV G'CCGG_C

Cuts at:      0    1017    2499 
   Size:       1017    1482
 
PacI TTA_AT'TAA

Cuts at:      0     638    2499 
   Size:        638    1861
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1198    2499 
   Size:       1198    1301
 
PfoI T'CCnGG_A

Cuts at:      0      61    2499 
   Size:         61    2438
 
PpuMI rG'GwC_Cy

Cuts at:      0    2181    2499 
   Size:       2181     318
 
PshAI GACnn'nnGTC

Cuts at:      0    2141    2499 
   Size:       2141     358
 
PspXI vC'TCGA_Gb

Cuts at:      0    1563    2262    2499 
   Size:       1563     699     237

  Fragments arranged by size: 

                1563     699     237
 
PstI C_TGCA'G

Cuts at:      0     588    2499 
   Size:        588    1911
 
SacI G_AGCT'C

Cuts at:      0    1054    2499 
   Size:       1054    1445
 
SacII CC_GC'GG

Cuts at:      0    2096    2499 
   Size:       2096     403
 
SexAI A'CCwGG_T

Cuts at:      0     233    2499 
   Size:        233    2266
 
SfcI C'TryA_G

Cuts at:      0     506     584    2499 
   Size:        506      78    1915

  Fragments arranged by size: 

                1915     506      78
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2349    2499 
   Size:       2349     150
 
SmaI CCC'GGG

Cuts at:      0     946    2499 
   Size:        946    1553
 
SmlI C'TyrA_G

Cuts at:      0    1563    2262    2499 
   Size:       1563     699     237

  Fragments arranged by size: 

                1563     699     237
 
SpeI A'CTAG_T

Cuts at:      0     342    2499 
   Size:        342    2157
 
StyI C'CwwG_G

Cuts at:      0    1397    2499 
   Size:       1397    1102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1113    2499 
   Size:       1113    1386
 
TatI w'GTAC_w

Cuts at:      0     345    1107    2499 
   Size:        345     762    1392

  Fragments arranged by size: 

                1392     762     345
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     239     271     447     479    1479    1511    1753    1785 
   Size:        239      32     176      32    1000      32     242      32

Cuts at:   1785    2499 
   Size:        714

  Fragments arranged by size: 

                1000     714     242     239     176      32      32      32
                  32
 
XhoI C'TCGA_G

Cuts at:      0    1563    2262    2499 
   Size:       1563     699     237

  Fragments arranged by size: 

                1563     699     237
 
XmaI C'CCGG_G

Cuts at:      0     944    2499 
   Size:        944    1555
 
XmnI GAAnn'nnTTC

Cuts at:      0     998    2499 
   Size:        998    1501
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AgeI     AleI    AlwNI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I   HincII     HpaI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     PacI
    PflMI     PfoI    PpuMI    PshAI    PspXI     PstI     SacI    SacII
    SexAI     SfcI    SgrAI     SmaI     SmlI     SpeI     StyI    TaqII
     TatI     TstI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsiEI    BsiWI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BstBI  BstZ17I     ClaI    CspCI     DraI     DrdI
     EagI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PasI     PciI     PmeI     PmlI     PpiI     PsiI
   PspOMI     PsrI     PvuI    PvuII    RsrII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SnaBI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     ZraI
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