Restriction map for
pF1KSDA1217
(Linear) (Six-base) MAPSORT of: KIAA1217.seq Check: 3189 from: 1 to: 3792
KIAA1217.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3456 3510 3792
Size: 3456 54 282
Fragments arranged by size:
3456 282 54
AatII G_ACGT'C
Cuts at: 0 190 2198 3792
Size: 190 2008 1594
Fragments arranged by size:
2008 1594 190
AccI GT'mk_AC
Cuts at: 0 706 1361 2972 3792
Size: 706 655 1611 820
Fragments arranged by size:
1611 820 706 655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 2158 2191 2326 3059 3792
Size: 70 2088 33 135 733 733
Fragments arranged by size:
2088 733 733 135 70 33
AfeI AGC'GCT
Cuts at: 0 1338 3792
Size: 1338 2454
AflIII A'CryG_T
Cuts at: 0 2217 3792
Size: 2217 1575
AhdI GACnn_n'nnGTC
Cuts at: 0 1870 3792
Size: 1870 1922
AleI CACnn'nnGTG
Cuts at: 0 1312 3792
Size: 1312 2480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1412 3421 3792
Size: 1412 2009 371
Fragments arranged by size:
2009 1412 371
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1715 1747 3384 3416 3792
Size: 1715 32 1637 32 376
Fragments arranged by size:
1715 1637 376 32 32
ApaI G_GGCC'C
Cuts at: 0 651 1514 3189 3792
Size: 651 863 1675 603
Fragments arranged by size:
1675 863 651 603
ApoI r'AATT_y
Cuts at: 0 693 746 2071 2853 3792
Size: 693 53 1325 782 939
Fragments arranged by size:
1325 939 782 693 53
BamHI G'GATC_C
Cuts at: 0 552 1851 3276 3792
Size: 552 1299 1425 516
Fragments arranged by size:
1425 1299 552 516
BanI G'GyrC_C
Cuts at: 0 760 1306 1644 1672 2359 2388 3688 3697
Size: 760 546 338 28 687 29 1300 9
Cuts at: 3697 3792
Size: 95
Fragments arranged by size:
1300 760 687 546 338 95 29 28
9
BanII G_rGCy'C
Cuts at: 0 124 651 1162 1514 2234 2428 3178 3189
Size: 124 527 511 352 720 194 750 11
Cuts at: 3189 3792
Size: 603
Fragments arranged by size:
750 720 603 527 511 352 194 124
11
BbsI GAAGACnn'nnnn_
Cuts at: 0 896 1952 1964 2021 3792
Size: 896 1056 12 57 1771
Fragments arranged by size:
1771 1056 896 57 12
BbvCI CC'TCA_GC
Cuts at: 0 1677 1780 3792
Size: 1677 103 2012
Fragments arranged by size:
2012 1677 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3186 3220 3792
Size: 3186 34 572
Fragments arranged by size:
3186 572 34
BfrBI ATG'CAT
Cuts at: 0 1092 3792
Size: 1092 2700
BglII A'GATC_T
Cuts at: 0 304 3792
Size: 304 3488
Bme1580I G_kGCm'C
Cuts at: 0 651 719 763 1514 1675 2364 3189 3700
Size: 651 68 44 751 161 689 825 511
Cuts at: 3700 3792
Size: 92
Fragments arranged by size:
825 751 689 651 511 161 92 68
44
BmgBI CAC'GTC
Cuts at: 0 2010 3792
Size: 2010 1782
BmrI ACTGGGnnnn_n'
Cuts at: 0 146 443 1165 2812 3792
Size: 146 297 722 1647 980
Fragments arranged by size:
1647 980 722 297 146
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3581 3792
Size: 3581 211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 695 798 813 1654 1717 1723 1741
Size: 310 385 103 15 841 63 6 18
Cuts at: 1741 1867 2794 3154 3162 3792
Size: 126 927 360 8 630
Fragments arranged by size:
927 841 630 385 360 310 126 103
63 18 15 8 6
Bpu10I CC'TnA_GC
Cuts at: 0 352 1677 1780 2741 3002 3792
Size: 352 1325 103 961 261 790
Fragments arranged by size:
1325 961 790 352 261 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1720 3792
Size: 1720 2072
BsaI GGTCTCn'nnnn_
Cuts at: 0 101 1426 3792
Size: 101 1325 2366
Fragments arranged by size:
2366 1325 101
BsaAI yAC'GTr
Cuts at: 0 2496 3792
Size: 2496 1296
BsaHI Gr'CG_yC
Cuts at: 0 187 2195 3792
Size: 187 2008 1597
Fragments arranged by size:
2008 1597 187
BsaWI w'CCGG_w
Cuts at: 0 1046 1134 3792
Size: 1046 88 2658
Fragments arranged by size:
2658 1046 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 796 826 1632 1662 1715 1745 2036 2066
Size: 796 30 806 30 53 30 291 30
Cuts at: 2066 3499 3529 3792
Size: 1433 30 263
Fragments arranged by size:
1433 806 796 291 263 53 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 647 1172 1364 2158 2332 2888 2891
Size: 523 124 525 192 794 174 556 3
Cuts at: 2891 2903 3079 3102 3105 3142 3636 3657 3792
Size: 12 176 23 3 37 494 21 135
Fragments arranged by size:
794 556 525 523 494 192 176 174
135 124 37 23 21 12 3 3
BseYI C'CCAG_C
Cuts at: 0 1651 2450 2508 2877 3382 3718 3792
Size: 1651 799 58 369 505 336 74
Fragments arranged by size:
1651 799 505 369 336 74 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 542 1610 1879 3792
Size: 398 144 1068 269 1913
Fragments arranged by size:
1913 1068 398 269 144
BsiEI CG_ry'CG
Cuts at: 0 170 3792
Size: 170 3622
BsiHKAI G_wGCw'C
Cuts at: 0 396 1632 2428 3624 3792
Size: 396 1236 796 1196 168
Fragments arranged by size:
1236 1196 796 396 168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 178 3678 3792
Size: 178 3500 114
Fragments arranged by size:
3500 178 114
Bsp1286I G_dGCh'C
Cuts at: 0 124 396 651 719 763 1162 1514 1632
Size: 124 272 255 68 44 399 352 118
Cuts at: 1632 1675 2234 2364 2428 3178 3189 3624 3700
Size: 43 559 130 64 750 11 435 76
Cuts at: 3700 3792
Size: 92
Fragments arranged by size:
750 559 435 399 352 272 255 130
124 118 92 76 68 64 44 43
11
BspEI T'CCGG_A
Cuts at: 0 1134 3792
Size: 1134 2658
BspHI T'CATG_A
Cuts at: 0 1905 2753 3792
Size: 1905 848 1039
Fragments arranged by size:
1905 1039 848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2936 3363 3456 3510 3792
Size: 2936 427 93 54 282
Fragments arranged by size:
2936 427 282 93 54
BsrBI CCG'CTC
Cuts at: 0 767 3792
Size: 767 3025
BsrDI GCAATG_nn'
Cuts at: 0 792 908 920 1022 1793 3792
Size: 792 116 12 102 771 1999
Fragments arranged by size:
1999 792 771 116 102 12
BsrFI r'CCGG_y
Cuts at: 0 2977 3792
Size: 2977 815
BsrGI T'GTAC_A
Cuts at: 0 542 917 1109 3792
Size: 542 375 192 2683
Fragments arranged by size:
2683 542 375 192
BssSI C'ACGA_G
Cuts at: 0 257 958 2952 3792
Size: 257 701 1994 840
Fragments arranged by size:
1994 840 701 257
BstEII G'GTnAC_C
Cuts at: 0 88 160 3580 3792
Size: 88 72 3420 212
Fragments arranged by size:
3420 212 88 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1609 1992 3792
Size: 1609 383 1800
Fragments arranged by size:
1800 1609 383
BstYI r'GATC_y
Cuts at: 0 304 552 661 1851 3276 3792
Size: 304 248 109 1190 1425 516
Fragments arranged by size:
1425 1190 516 304 248 109
Bsu36I CC'TnA_GG
Cuts at: 0 1201 3792
Size: 1201 2591
BtgI C'CryG_G
Cuts at: 0 770 987 1236 1330 1454 1640 1976 2459
Size: 770 217 249 94 124 186 336 483
Cuts at: 2459 3792
Size: 1333
Fragments arranged by size:
1333 770 483 336 249 217 186 124
94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 350 2384 2978 3792
Size: 350 2034 594 814
Fragments arranged by size:
2034 814 594 350
BtsI GCAGTG_nn'
Cuts at: 0 2651 2797 3018 3792
Size: 2651 146 221 774
Fragments arranged by size:
2651 774 221 146
ClaI AT'CG_AT
Cuts at: 0 3463 3792
Size: 3463 329
DraI TTT'AAA
Cuts at: 0 1293 1506 3792
Size: 1293 213 2286
Fragments arranged by size:
2286 1293 213
DraIII CAC_nnn'GTG
Cuts at: 0 2460 2625 3792
Size: 2460 165 1167
Fragments arranged by size:
2460 1167 165
EaeI y'GGCC_r
Cuts at: 0 2337 2498 3615 3792
Size: 2337 161 1117 177
Fragments arranged by size:
2337 1117 177 161
EarI CTCTTCn'nnn_
Cuts at: 0 19 403 864 1834 1907 2302 3417 3792
Size: 19 384 461 970 73 395 1115 375
Fragments arranged by size:
1115 970 461 395 384 375 73 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1725 1735 2894 3792
Size: 1725 10 1159 898
Fragments arranged by size:
1725 1159 898 10
EcoICRI GAG'CTC
Cuts at: 0 2426 3792
Size: 2426 1366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 310 695 798 813 1654 1717 1723
Size: 70 240 385 103 15 841 63 6
Cuts at: 1723 1741 1867 2158 2191 2326 2794 3059 3154
Size: 18 126 291 33 135 468 265 95
Cuts at: 3154 3162 3792
Size: 8 630
Fragments arranged by size:
841 630 468 385 291 265 240 135
126 103 95 70 63 33 18 15
8 6
EcoO109I rG'GnC_Cy
Cuts at: 0 647 648 1510 3185 3186 3792
Size: 647 1 862 1675 1 606
Fragments arranged by size:
1675 862 647 606 1 1
EcoRI G'AATT_C
Cuts at: 0 746 3792
Size: 746 3046
FspI TGC'GCA
Cuts at: 0 2110 2182 3792
Size: 2110 72 1610
Fragments arranged by size:
2110 1610 72
HaeII r_GCGC'y
Cuts at: 0 857 1340 3792
Size: 857 483 2452
Fragments arranged by size:
2452 857 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 518 550 796 828 1255 1287
Size: 163 32 323 32 246 32 427 32
Cuts at: 1287 1856 1888 2362 2394 2927 2959 3792
Size: 569 32 474 32 533 32 833
Fragments arranged by size:
833 569 533 474 427 323 246 163
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 284 3792
Size: 284 3508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1922 2101 2360 3792
Size: 253 1669 179 259 1432
Fragments arranged by size:
1669 1432 259 253 179
MscI TGG'CCA
Cuts at: 0 2500 3792
Size: 2500 1292
MslI CAynn'nnrTG
Cuts at: 0 433 604 913 1229 1312 1687 2116 2275
Size: 433 171 309 316 83 375 429 159
Cuts at: 2275 2705 3792
Size: 430 1087
Fragments arranged by size:
1087 433 430 429 375 316 309 171
159 83
MspA1I CmG'CkG
Cuts at: 0 193 1761 3182 3792
Size: 193 1568 1421 610
Fragments arranged by size:
1568 1421 610 193
NcoI C'CATG_G
Cuts at: 0 770 987 1454 3792
Size: 770 217 467 2338
Fragments arranged by size:
2338 770 467 217
NdeI CA'TA_TG
Cuts at: 0 1094 3792
Size: 1094 2698
NsiI A_TGCA'T
Cuts at: 0 1094 3792
Size: 1094 2698
NspI r_CATG'y
Cuts at: 0 565 1218 2221 3792
Size: 565 653 1003 1571
Fragments arranged by size:
1571 1003 653 565
PasI CC'CwG_GG
Cuts at: 0 643 800 3792
Size: 643 157 2992
Fragments arranged by size:
2992 643 157
PciI A'CATG_T
Cuts at: 0 2217 3792
Size: 2217 1575
PmlI CAC'GTG
Cuts at: 0 2496 3792
Size: 2496 1296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1715 1747 3792
Size: 1715 32 2045
Fragments arranged by size:
2045 1715 32
PshAI GACnn'nnGTC
Cuts at: 0 2194 3792
Size: 2194 1598
PspOMI G'GGCC_C
Cuts at: 0 647 1510 3185 3792
Size: 647 863 1675 607
Fragments arranged by size:
1675 863 647 607
PstI C_TGCA'G
Cuts at: 0 1344 1766 2543 3275 3792
Size: 1344 422 777 732 517
Fragments arranged by size:
1344 777 732 517 422
PvuII CAG'CTG
Cuts at: 0 1761 3792
Size: 1761 2031
SacI G_AGCT'C
Cuts at: 0 2428 3792
Size: 2428 1364
SfcI C'TryA_G
Cuts at: 0 1340 1401 1762 2051 2539 3271 3792
Size: 1340 61 361 289 488 732 521
Fragments arranged by size:
1340 732 521 488 361 289 61
SmlI C'TyrA_G
Cuts at: 0 1699 3792
Size: 1699 2093
StuI AGG'CCT
Cuts at: 0 1288 3792
Size: 1288 2504
StyI C'CwwG_G
Cuts at: 0 770 987 1310 1454 2803 3692 3792
Size: 770 217 323 144 1349 889 100
Fragments arranged by size:
1349 889 770 323 217 144 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 165 1013 3792
Size: 148 17 848 2779
Fragments arranged by size:
2779 848 148 17
TatI w'GTAC_w
Cuts at: 0 542 917 1109 2267 3792
Size: 542 375 192 1158 1525
Fragments arranged by size:
1525 1158 542 375 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 992 1024 1632 1664 3499 3531 3792
Size: 992 32 608 32 1835 32 261
Fragments arranged by size:
1835 992 608 261 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 166 3792
Size: 166 3626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 435 1609 3792
Size: 435 1174 2183
Fragments arranged by size:
2183 1174 435
ZraI GAC'GTC
Cuts at: 0 188 2196 3792
Size: 188 2008 1596
Fragments arranged by size:
2008 1596 188
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AloI ApaI ApoI BamHI BanI BanII BbsI
BbvCI BcgI BfrBI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PciI PmlI PpiI
PshAI PspOMI PstI PvuII SacI SfcI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BciVI BclI BglI
BlpI BmtI BsaBI BsiWI BsmI BssHII BstAPI BstBI
BstZ17I CspCI DrdI EagI EcoNI EcoRV FalI FseI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PflMI PfoI
PmeI PpuMI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI XmnI