Restriction map for pF1KSDA1215
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AflIII      
AgeI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AvrII       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       PvuI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        XbaI        
XhoI        XmnI        


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# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          853  GTCTAC               GT^[AC][GT]AC
AclI          784  AACGTT               AA^CGTT
Alw44I        685  GTGCAC               G^TGCAC
ApaLI         685  GTGCAC               G^TGCAC
AspI          289  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          766  GGCGCC               GGCGC^C
BglI          337  GCCACCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1036  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1036  GCTGAGC              GC^T[ACGT]AGC
BsgI          646  GTGCAG               GTGCAG(16/14)
BspHI        1535  TCATGA               T^CATGA
BspMI         875  ACCTGC               ACCTGC(4/8)
BstEII       1401  GGTGACC              G^GT[ACGT]ACC
BstPI        1401  GGTGACC              G^GT[ACGT]ACC
CelII        1036  GCTGAGC              GC^T[ACGT]AGC
DrdI          844  GACTTCCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         927  CCTCGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1401  GGTGACC              G^GT[ACGT]ACC
EcoRI        1273  GAATTC               G^AATTC
HaeII         766  GGCGCC               [GA]GCGC^[CT]
KasI          766  GGCGCC               G^GCGCC
MaeII         785  ACGT                 A^CGT
NaeI          506  GCCGGC               GCC^GGC
NarI          766  GGCGCC               GG^CGCC
NgoMIV        506  GCCGGC               G^CCGGC
PflFI         289  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1478  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        1003  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      784  AACGTT               AA^CGTT
PvuII         486  CAGCTG               CAG^CTG
RcaI         1535  TCATGA               T^CATGA
SapI          591  GCTCTTC              GCTCTTC(1/4)
SfoI          766  GGCGCC               GGC^GCC
TaqI          102  TCGA                 T^CGA
Tth111I       289  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       102  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccII         529  CGCG                 CG^CG
              808  CGCG                
AcsI         1273  GAATTC               [GA]^AATT[CT]
             1504  GAATTT              
AhdI            4  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              163  GACAATGAGTC         
ApoI         1273  GAATTC               [GA]^AATT[CT]
             1504  GAATTT              
AspEI           4  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              163  GACAATGAGTC         
AspHI         238  GAGCAC               G[AT]GC[AT]^C
              685  GTGCAC              
AviII         605  TGCGCA               TGC^GCA
             1055  TGCGCA              
BamHI         821  GGATCC               G^GATCC
             1512  GGATCC              
BanI          229  GGCACC               G^G[CT][GA]CC
              766  GGCGCC              
BanII         151  GAGCCC               G[GA]GC[CT]^C
              491  GGGCTC              
BsaI         1552  GAGACC               GGTCTC(1/5)
              579  GGTCTC              
BsiHKAI       238  GAGCAC               G[AT]GC[AT]^C
              685  GTGCAC              
BsrBI          90  CCGCTC               CCGCTC(-3/-3)
              187  GAGCGG              
BsrFI         506  GCCGGC               [GA]^CCGG[CT]
             1398  GCCGGT              
BssSI         176  CACGAG               CACGAG(-5/-1)
              574  CTCGTG              
BstUI         529  CGCG                 CG^CG
              808  CGCG                
BstXI         275  CCAAGGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              391  CCACTGCTGTGG        
BstYI         821  GGATCC               [GA]^GATC[CT]
             1512  GGATCC              
Cfr10I        506  GCCGGC               [GA]^CCGG[CT]
             1398  GCCGGT              
DpnII         822  GATC                 ^GATC
             1513  GATC                
DraII         437  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1478  AGGTCCC             
Eam1105I        4  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              163  GACAATGAGTC         
EcoO109I      437  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1478  AGGTCCC             
FspI          605  TGCGCA               TGC^GCA
             1055  TGCGCA              
MboI          822  GATC                 ^GATC
             1513  GATC                
MflI          821  GGATCC               [GA]^GATC[CT]
             1512  GGATCC              
MseI         1109  TTAA                 T^TAA
             1438  TTAA                
MslI          316  CACCTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              392  CACTGCTGTG          
MvnI          529  CGCG                 CG^CG
              808  CGCG                
NcoI         1190  CCATGG               C^CATGG
             1247  CCATGG              
NdeII         822  GATC                 ^GATC
             1513  GATC                
Sau3AI        822  GATC                 ^GATC
             1513  GATC                
ScaI           17  AGTACT               AGT^ACT
             1019  AGTACT              
SmaI          145  CCCGGG               CCC^GGG
             1160  CCCGGG              
SmlI          742  CTCAAG               C^T[CT][GA]AG
             1414  CTCAAG              
Tru9I        1109  TTAA                 T^TAA
             1438  TTAA                
Tsp509I      1274  AATT                 ^AATT
             1505  AATT                
XhoII         821  GGATCC               [GA]^GATC[CT]
             1512  GGATCC              
XmaCI         145  CCCGGG               C^CCGGG
             1160  CCCGGG              
XmaI          145  CCCGGG               C^CCGGG
             1160  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AvaI        AvaII       BalI        BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DsaI        
EaeI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboII       
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PflMI       PleI        PstI        RsaI        Sau96I      
ScrFI       SfaNI       SfcI        StuI        StyI        
TseI        Tsp45I      TspRI       Van91I      XcmI        
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