Restriction map for pF1KSDA1215
 (Linear) (Six-base) MAPSORT of: KIAA1215.seq  Check: 5430  from: 1  to: 1563



KIAA1215.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AccI GT'mk_AC

Cuts at:      0     854    1563 
   Size:        854     709
 
AclI AA'CG_TT

Cuts at:      0     785    1563 
   Size:        785     778
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1437    1563 
   Size:       1437     126
 
AhdI GACnn_n'nnGTC

Cuts at:      0       9     168    1563 
   Size:          9     159    1395

  Fragments arranged by size: 

                1395     159       9
 
AleI CACnn'nnGTG

Cuts at:      0     320     396    1563 
   Size:        320      76    1167

  Fragments arranged by size: 

                1167     320      76
 
ApaLI G'TGCA_C

Cuts at:      0     685    1563 
   Size:        685     878
 
ApoI r'AATT_y

Cuts at:      0    1273    1504    1563 
   Size:       1273     231      59

  Fragments arranged by size: 

                1273     231      59
 
AvaI C'yCGr_G

Cuts at:      0      21     145     620     968    1160    1563 
   Size:         21     124     475     348     192     403

  Fragments arranged by size: 

                 475     403     348     192     124      21
 
BamHI G'GATC_C

Cuts at:      0     821    1512    1563 
   Size:        821     691      51

  Fragments arranged by size: 

                 821     691      51
 
BanI G'GyrC_C

Cuts at:      0     229     766    1563 
   Size:        229     537     797

  Fragments arranged by size: 

                 797     537     229
 
BanII G_rGCy'C

Cuts at:      0     155     495    1563 
   Size:        155     340    1068

  Fragments arranged by size: 

                1068     340     155
 
BbeI G_GCGC'C

Cuts at:      0     770    1563 
   Size:        770     793
 
BglI GCCn_nnn'nGGC

Cuts at:      0     343    1563 
   Size:        343    1220
 
BlpI GC'TnA_GC

Cuts at:      0    1037    1563 
   Size:       1037     526
 
Bme1580I G_kGCm'C

Cuts at:      0     232     549     689    1563 
   Size:        232     317     140     874

  Fragments arranged by size: 

                 874     317     232     140
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       9     210     490    1563 
   Size:          9     201     280    1073

  Fragments arranged by size: 

                1073     280     201       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     709     730     847    1563 
   Size:        433     276      21     117     716

  Fragments arranged by size: 

                 716     433     276     117      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727    1399    1563 
   Size:        727     672     164

  Fragments arranged by size: 

                 727     672     164
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     585    1546    1563 
   Size:        585     961      17

  Fragments arranged by size: 

                 961     585      17
 
BsaHI Gr'CG_yC

Cuts at:      0     671     767    1297    1563 
   Size:        671      96     530     266

  Fragments arranged by size: 

                 671     530     266      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     232     262    1563 
   Size:        232      30    1301

  Fragments arranged by size: 

                1301     232      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     421     859    1045    1077    1105    1141    1408    1563 
   Size:        421     438     186      32      28      36     267     155

  Fragments arranged by size: 

                 438     421     267     186     155      36      32      28
            
 
BseYI C'CCAG_C

Cuts at:      0     488    1563 
   Size:        488    1075
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     667    1563 
   Size:        667     896
 
BsiHKAI G_wGCw'C

Cuts at:      0     242     689    1563 
   Size:        242     447     874

  Fragments arranged by size: 

                 874     447     242
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     232     242     495     549     689    1563 
   Size:        155      77      10     253      54     140     874

  Fragments arranged by size: 

                 874     253     155     140      77      54      10
 
BspHI T'CATG_A

Cuts at:      0    1535    1563 
   Size:       1535      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     884    1563 
   Size:        884     679
 
BsrBI CCG'CTC

Cuts at:      0      92     189    1563 
   Size:         92      97    1374

  Fragments arranged by size: 

                1374      97      92
 
BsrFI r'CCGG_y

Cuts at:      0     506    1398    1563 
   Size:        506     892     165

  Fragments arranged by size: 

                 892     506     165
 
BssSI C'ACGA_G

Cuts at:      0     176     574    1563 
   Size:        176     398     989

  Fragments arranged by size: 

                 989     398     176
 
BstEII G'GTnAC_C

Cuts at:      0    1401    1563 
   Size:       1401     162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     282     398    1563 
   Size:        282     116    1165

  Fragments arranged by size: 

                1165     282     116
 
BstYI r'GATC_y

Cuts at:      0     821    1512    1563 
   Size:        821     691      51

  Fragments arranged by size: 

                 821     691      51
 
BtgI C'CryG_G

Cuts at:      0     698    1190    1247    1563 
   Size:        698     492      57     316

  Fragments arranged by size: 

                 698     492     316      57
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     255    1563 
   Size:        255    1308
 
BtsI GCAGTG_nn'

Cuts at:      0     346     391     821    1563 
   Size:        346      45     430     742

  Fragments arranged by size: 

                 742     430     346      45
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     850    1563 
   Size:        850     713
 
EaeI y'GGCC_r

Cuts at:      0     695     789     893     960    1367    1563 
   Size:        695      94     104      67     407     196

  Fragments arranged by size: 

                 695     407     196     104      94      67
 
EarI CTCTTCn'nnn_

Cuts at:      0     448     598    1118    1563 
   Size:        448     150     520     445

  Fragments arranged by size: 

                 520     448     445     150
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     493    1563 
   Size:        493    1070
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     709     730     847    1437    1563 
   Size:        433     276      21     117     590     126

  Fragments arranged by size: 

                 590     433     276     126     117      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     931    1563 
   Size:        931     632
 
EcoO109I rG'GnC_Cy

Cuts at:      0     438    1479    1563 
   Size:        438    1041      84

  Fragments arranged by size: 

                1041     438      84
 
EcoRI G'AATT_C

Cuts at:      0    1273    1563 
   Size:       1273     290
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1326    1563 
   Size:       1326     237
 
FspI TGC'GCA

Cuts at:      0     607    1057    1563 
   Size:        607     450     506

  Fragments arranged by size: 

                 607     506     450
 
FspAI rTGC'GCAy

Cuts at:      0     607    1563 
   Size:        607     956
 
HaeII r_GCGC'y

Cuts at:      0     770    1563 
   Size:        770     793
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     154     186    1325    1357    1504    1536    1563 
   Size:         27     127      32    1139      32     147      32      27

  Fragments arranged by size: 

                1139     147     127      32      32      32      27      27
            
 
KasI G'GCGC_C

Cuts at:      0     766    1563 
   Size:        766     797
 
MscI TGG'CCA

Cuts at:      0     791     895     962    1369    1563 
   Size:        791     104      67     407     194

  Fragments arranged by size: 

                 791     407     194     104      67
 
MslI CAynn'nnrTG

Cuts at:      0     320     396    1563 
   Size:        320      76    1167

  Fragments arranged by size: 

                1167     320      76
 
MspA1I CmG'CkG

Cuts at:      0     331     488     985    1563 
   Size:        331     157     497     578

  Fragments arranged by size: 

                 578     497     331     157
 
NaeI GCC'GGC

Cuts at:      0     508    1563 
   Size:        508    1055
 
NarI GG'CG_CC

Cuts at:      0     767    1563 
   Size:        767     796
 
NcoI C'CATG_G

Cuts at:      0    1190    1247    1563 
   Size:       1190      57     316

  Fragments arranged by size: 

                1190     316      57
 
NgoMIV G'CCGG_C

Cuts at:      0     506    1563 
   Size:        506    1057
 
PasI CC'CwG_GG

Cuts at:      0    1142    1563 
   Size:       1142     421
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     642     810    1248    1376    1563 
   Size:        642     168     438     128     187

  Fragments arranged by size: 

                 642     438     187     168     128
 
PfoI T'CCnGG_A

Cuts at:      0     611     824    1563 
   Size:        611     213     739

  Fragments arranged by size: 

                 739     611     213
 
PpuMI rG'GwC_Cy

Cuts at:      0    1479    1563 
   Size:       1479      84
 
PshAI GACnn'nnGTC

Cuts at:      0    1007    1563 
   Size:       1007     556
 
PstI C_TGCA'G

Cuts at:      0    1121    1265    1547    1563 
   Size:       1121     144     282      16

  Fragments arranged by size: 

                1121     282     144      16
 
PvuII CAG'CTG

Cuts at:      0     488    1563 
   Size:        488    1075
 
SapI GCTCTTCn'nnn_

Cuts at:      0     598    1563 
   Size:        598     965
 
ScaI AGT'ACT

Cuts at:      0      19    1021    1563 
   Size:         19    1002     542

  Fragments arranged by size: 

                1002     542      19
 
SfcI C'TryA_G

Cuts at:      0    1117    1261    1543    1563 
   Size:       1117     144     282      20

  Fragments arranged by size: 

                1117     282     144      20
 
SfoI GGC'GCC

Cuts at:      0     768    1563 
   Size:        768     795
 
SmaI CCC'GGG

Cuts at:      0     147    1162    1563 
   Size:        147    1015     401

  Fragments arranged by size: 

                1015     401     147
 
SmlI C'TyrA_G

Cuts at:      0     742    1414    1563 
   Size:        742     672     149

  Fragments arranged by size: 

                 742     672     149
 
StuI AGG'CCT

Cuts at:      0     517    1096    1306    1563 
   Size:        517     579     210     257

  Fragments arranged by size: 

                 579     517     257     210
 
StyI C'CwwG_G

Cuts at:      0     275     512    1190    1247    1301    1563 
   Size:        275     237     678      57      54     262

  Fragments arranged by size: 

                 678     275     262     237      57      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      98     310    1563 
   Size:         98     212    1253

  Fragments arranged by size: 

                1253     212      98
 
TatI w'GTAC_w

Cuts at:      0      17    1019    1563 
   Size:         17    1002     544

  Fragments arranged by size: 

                1002     544      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     232     264    1563 
   Size:        232      32    1299

  Fragments arranged by size: 

                1299     232      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     292    1563 
   Size:        292    1271
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     888    1077    1149    1308    1563 
   Size:        888     189      72     159     255

  Fragments arranged by size: 

                 888     255     189     159      72
 
XmaI C'CCGG_G

Cuts at:      0     145    1160    1563 
   Size:        145    1015     403

  Fragments arranged by size: 

                1015     403     145
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AhdI     AleI    ApaLI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI     BglI     BlpI Bme1580I     BmrI
     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BssSI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    FspAI
    HaeII    Hin4I     KasI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     PasI    PflMI     PfoI    PpuMI    PshAI     PstI
    PvuII     SapI     ScaI     SfcI     SfoI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AlfI
     AloI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     EagI  EcoICRI
    EcoRV     FseI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     XbaI     XhoI     XmnI     ZraI
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