Restriction map for pF1KSDA1214
 (Linear) (Six-base) MAPSORT of: KIAA1214.seq  Check: 4358  from: 1  to: 1188



KIAA1214.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1002    1188 
   Size:       1002     186
 
AatII G_ACGT'C

Cuts at:      0    1055    1188 
   Size:       1055     133
 
AflIII A'CryG_T

Cuts at:      0     401    1188 
   Size:        401     787
 
AleI CACnn'nnGTG

Cuts at:      0     482     955    1188 
   Size:        482     473     233

  Fragments arranged by size: 

                 482     473     233
 
AlwNI CAG_nnn'CTG

Cuts at:      0     691    1188 
   Size:        691     497
 
AvaI C'yCGr_G

Cuts at:      0     163    1188 
   Size:        163    1025
 
AvrII C'CTAG_G

Cuts at:      0     849    1188 
   Size:        849     339
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     795     828    1188 
   Size:        795      33     360

  Fragments arranged by size: 

                 795     360      33
 
BamHI G'GATC_C

Cuts at:      0     749     854    1188 
   Size:        749     105     334

  Fragments arranged by size: 

                 749     334     105
 
BanI G'GyrC_C

Cuts at:      0     940    1188 
   Size:        940     248
 
BanII G_rGCy'C

Cuts at:      0     152     448     907    1188 
   Size:        152     296     459     281

  Fragments arranged by size: 

                 459     296     281     152
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     217     497    1188 
   Size:        217     280     691

  Fragments arranged by size: 

                 691     280     217
 
BbvCI CC'TCA_GC

Cuts at:      0     420    1188 
   Size:        420     768
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     589     623    1188 
   Size:        589      34     565

  Fragments arranged by size: 

                 589     565      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1017    1188 
   Size:       1017     171
 
BclI T'GATC_A

Cuts at:      0    1115    1188 
   Size:       1115      73
 
BglI GCCn_nnn'nGGC

Cuts at:      0     175     283     762    1188 
   Size:        175     108     479     426

  Fragments arranged by size: 

                 479     426     175     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     625    1188 
   Size:        625     563
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634    1188 
   Size:        634     554
 
Bpu10I CC'TnA_GC

Cuts at:      0     420     515    1188 
   Size:        420      95     673

  Fragments arranged by size: 

                 673     420      95
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     454    1188 
   Size:        454     734
 
BsaAI yAC'GTr

Cuts at:      0     380    1188 
   Size:        380     808
 
BsaHI Gr'CG_yC

Cuts at:      0     254    1052    1188 
   Size:        254     798     136

  Fragments arranged by size: 

                 798     254     136
 
BsaWI w'CCGG_w

Cuts at:      0     395     746     992    1188 
   Size:        395     351     246     196

  Fragments arranged by size: 

                 395     351     246     196
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     100     130     430     460    1188 
   Size:        100      30     300      30     728

  Fragments arranged by size: 

                 728     300     100      30      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     360     728    1188 
   Size:        184     176     368     460

  Fragments arranged by size: 

                 460     368     184     176
 
BsiEI CG_ry'CG

Cuts at:      0     294    1188 
   Size:        294     894
 
BsiHKAI G_wGCw'C

Cuts at:      0     239    1188 
   Size:        239     949
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1043    1188 
   Size:       1043     145
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     239     448     907    1188 
   Size:        152      87     209     459     281

  Fragments arranged by size: 

                 459     281     209     152      87
 
BspEI T'CCGG_A

Cuts at:      0     395     746     992    1188 
   Size:        395     351     246     196

  Fragments arranged by size: 

                 395     351     246     196
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1002    1188 
   Size:       1002     186
 
BsrDI GCAATG_nn'

Cuts at:      0      11    1130    1188 
   Size:         11    1119      58

  Fragments arranged by size: 

                1119      58      11
 
BsrFI r'CCGG_y

Cuts at:      0     761    1188 
   Size:        761     427
 
BsrGI T'GTAC_A

Cuts at:      0     572    1188 
   Size:        572     616
 
BstYI r'GATC_y

Cuts at:      0     392     749     854    1188 
   Size:        392     357     105     334

  Fragments arranged by size: 

                 392     357     334     105
 
BtgI C'CryG_G

Cuts at:      0      95     258     336     425    1188 
   Size:         95     163      78      89     763

  Fragments arranged by size: 

                 763     163      95      89      78
 
BtsI GCAGTG_nn'

Cuts at:      0    1101    1188 
   Size:       1101      87
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     988    1188 
   Size:        988     200
 
EaeI y'GGCC_r

Cuts at:      0      98     275     500    1188 
   Size:         98     177     225     688

  Fragments arranged by size: 

                 688     225     177      98
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634    1188 
   Size:        634     554
 
HaeII r_GCGC'y

Cuts at:      0     228    1188 
   Size:        228     960
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1040    1072    1188 
   Size:       1040      32     116

  Fragments arranged by size: 

                1040     116      32
 
HincII GTy'rAC

Cuts at:      0     789    1188 
   Size:        789     399
 
MluI A'CGCG_T

Cuts at:      0     401    1188 
   Size:        401     787
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     473    1113    1188 
   Size:        473     640      75

  Fragments arranged by size: 

                 640     473      75
 
MscI TGG'CCA

Cuts at:      0     277     502    1188 
   Size:        277     225     686

  Fragments arranged by size: 

                 686     277     225
 
MslI CAynn'nnrTG

Cuts at:      0     469     482     562     955    1123    1188 
   Size:        469      13      80     393     168      65

  Fragments arranged by size: 

                 469     393     168      80      65      13
 
MspA1I CmG'CkG

Cuts at:      0     260     338    1087    1188 
   Size:        260      78     749     101

  Fragments arranged by size: 

                 749     260     101      78
 
NaeI GCC'GGC

Cuts at:      0     763    1188 
   Size:        763     425
 
NgoMIV G'CCGG_C

Cuts at:      0     761    1188 
   Size:        761     427
 
PfoI T'CCnGG_A

Cuts at:      0    1019    1188 
   Size:       1019     169
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     124     156    1188 
   Size:        124      32    1032

  Fragments arranged by size: 

                1032     124      32
 
PstI C_TGCA'G

Cuts at:      0      31     413    1188 
   Size:         31     382     775

  Fragments arranged by size: 

                 775     382      31
 
PvuII CAG'CTG

Cuts at:      0    1087    1188 
   Size:       1087     101
 
SacII CC_GC'GG

Cuts at:      0     261     339    1188 
   Size:        261      78     849

  Fragments arranged by size: 

                 849     261      78
 
SfcI C'TryA_G

Cuts at:      0      27     409    1188 
   Size:         27     382     779

  Fragments arranged by size: 

                 779     382      27
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     283    1188 
   Size:        283     905
 
SmaI CCC'GGG

Cuts at:      0     165    1188 
   Size:        165    1023
 
StyI C'CwwG_G

Cuts at:      0     365     849    1188 
   Size:        365     484     339

  Fragments arranged by size: 

                 484     365     339
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     936    1188 
   Size:        936     252
 
TatI w'GTAC_w

Cuts at:      0     572    1188 
   Size:        572     616
 
Tth111I GACn'n_nGTC

Cuts at:      0     742    1188 
   Size:        742     446
 
XbaI T'CTAG_A

Cuts at:      0     801    1188 
   Size:        801     387
 
XmaI C'CCGG_G

Cuts at:      0     163    1188 
   Size:        163    1025
 
XmnI GAAnn'nnTTC

Cuts at:      0     403    1188 
   Size:        403     785
 
ZraI GAC'GTC

Cuts at:      0    1053    1188 
   Size:       1053     135
 

 Enzymes that do cut:

     AarI    AatII   AflIII     AleI    AlwNI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrDI    BsrFI    BsrGI    BstYI     BtgI     BtsI     DrdI     EaeI
  Eco57MI    HaeII    Hin4I   HincII     MluI     MmeI     MscI     MslI
   MspA1I     NaeI   NgoMIV     PfoI     PsrI     PstI    PvuII    SacII
     SfcI     SfiI     SmaI     StyI    TaqII     TatI  Tth111I     XbaI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     BbeI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
    BpuEI    BsaBI    BseRI    BseYI    BsiWI     BsmI    BspHI    BsrBI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XcmI
     XhoI
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