Restriction map for pF1KSDA1201
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AflII       AgeI        AlwNI       AocI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BseAI       BsiWI       BspDI       
BspEI       BspHI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DrdI        Eco47III    
Eco81I      EcoT22I     FbaI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AclI         1419  AACGTT               AA^CGTT
AcsI          105  GAATTC               [GA]^AATT[CT]
Alw44I        230  GTGCAC               G^TGCAC
ApaLI         230  GTGCAC               G^TGCAC
ApoI          105  GAATTC               [GA]^AATT[CT]
AvaI          443  CCCGGG               C^[CT]CG[GA]G
BalI         1484  TGGCCA               TGG^CCA
BciVI         616  GGATAC               GTATCC(6/5)
BglI           89  GCCGGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI          48  ACCGGA               [AT]^CCGG[AT]
BsgI           61  GTGCAG               GTGCAG(16/14)
BsiEI          87  CGGCCG               CG[GA][CT]^CG
BsmI          483  GAATGC               GAATGC(1/-1)
BsoBI         443  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1232  TGTACA               T^GTACA
BspLU11I     1668  ACATGT               A^CATGT
BsrBI          93  GAGCGG               CCGCTC(-3/-3)
BsrFI         998  GCCGGC               [GA]^CCGG[CT]
BsrGI        1232  TGTACA               T^GTACA
BstAPI         54  GCAGGGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        998  GCCGGC               [GA]^CCGG[CT]
DraIII       1288  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1123  CCATGG               C^C[GA][CT]GG
EagI           87  CGGCCG               C^GGCCG
EclXI          87  CGGCCG               C^GGCCG
Eco52I         87  CGGCCG               C^GGCCG
EcoRI         105  GAATTC               G^AATTC
EcoRV        1108  GATATC               GAT^ATC
MluNI        1484  TGGCCA               TGG^CCA
MscI         1484  TGGCCA               TGG^CCA
MslI         1338  CACCCGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          998  GCCGGC               GCC^GGC
NcoI         1123  CCATGG               C^CATGG
NgoMIV        998  GCCGGC               G^CCGGC
NspI         1668  ACATGT               [GA]CATG^[CT]
Psp1406I     1419  AACGTT               AA^CGTT
PstI         1729  CTGCAG               CTGCA^G
SapI         1812  GCTCTTC              GCTCTTC(1/4)
SmaI          443  CCCGGG               CCC^GGG
SspBI        1232  TGTACA               T^GTACA
StuI          974  AGGCCT               AGG^CCT
XcmI         1742  CCAAGCTGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         443  CCCGGG               C^CCGGG
XmaI          443  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII        1691  CGCG                 CG^CG
             2050  CGCG                
AflIII       1668  ACATGT               A^C[GA][CT]GT
             2057  ACACGT              
AhdI         1364  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2053  GACTACACGTC         
ApaI          440  GGGCCC               GGGCC^C
             1913  GGGCCC              
Asp700I      1416  GAAAACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1431  GAAGAACTTC          
AspEI        1364  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2053  GACTACACGTC         
BbsI          166  GAAGAC               GAAGAC(2/6)
             1018  GTCTTC              
BpuAI         166  GAAGAC               GAAGAC(2/6)
             1018  GTCTTC              
BsaI          682  GAGACC               GGTCTC(1/5)
             1371  GGTCTC              
BsmBI         768  CGTCTC               CGTCTC(1/5)
              812  GAGACG              
BstEII       1626  GGTCACC              G^GT[ACGT]ACC
             1889  GGTTACC             
BstPI        1626  GGTCACC              G^GT[ACGT]ACC
             1889  GGTTACC             
BstUI        1691  CGCG                 CG^CG
             2050  CGCG                
BstXI         537  CCAGTGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1184  CCAACCCTCTGG        
BstYI         284  GGATCT               [GA]^GATC[CT]
              629  AGATCC              
Eam1105I     1364  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2053  GACTACACGTC         
EcoNI         822  CCTGGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1605  CCTGGAAGAGG         
EcoO65I      1626  GGTCACC              G^GT[ACGT]ACC
             1889  GGTTACC             
EcoT14I       512  CCAAGG               C^C[AT][AT]GG
             1123  CCATGG              
FauI         2048  CCCGC                CCCGC(4/6)
             1077  GCGGG               
HgaI         1689  GACGC                GACGC(5/10)
             1575  GCGTC               
MflI          284  GGATCT               [GA]^GATC[CT]
              629  AGATCC              
MvnI         1691  CGCG                 CG^CG
             2050  CGCG                
SfcI          294  CTACAG               C^T[GA][CT]AG
             1729  CTGCAG              
StyI          512  CCAAGG               C^C[AT][AT]GG
             1123  CCATGG              
TaqI         1392  TCGA                 T^CGA
             1427  TCGA                
Tsp45I        938  GTGAC                ^GT[GC]AC
             1627  GTCAC               
TthHB8I      1392  TCGA                 T^CGA
             1427  TCGA                
XhoII         284  GGATCT               [GA]^GATC[CT]
              629  AGATCC              
XmnI         1416  GAAAACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1431  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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