Restriction map for pF1KSDA1197
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       AccI        AccII       AcyI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AspEI       AspHI       AspI        
AviII       AvrII       BbeI        BfrI        BlnI        
BsaHI       BsiEI       BsiHKAI     BsiWI       Bsp1407I    
BspDI       BspHI       BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoT22I     FseI        FspI        
Hin1I       HpaI        KasI        KspI        MluI        
MvnI        NarI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PshAI       PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       1956  GGTACC               G^GTACC
AclI         2214  AACGTT               AA^CGTT
Asp718I      1956  GGTACC               G^GTACC
BamHI        2191  GGATCC               G^GATCC
BanII        3778  GAGCCC               G[GA]GC[CT]^C
BbvCI        3888  CCTCAGC              CCTCAGC(-5/-2)
BcgI         3203  CGATCCTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1766  TGATCA               T^GATCA
BcnI         1516  CCGGG                CC^[GC]GG
BglI         2242  GCCAACTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1568  AGATCT               A^GATCT
BsmBI         964  GAGACG               CGTCTC(1/5)
BsmI          579  GAATGC               GAATGC(1/-1)
BspLU11I      301  ACATGT               A^CATGT
BsrFI        1471  GCCGGC               [GA]^CCGG[CT]
BssSI        3052  CTCGTG               CACGAG(-5/-1)
BstAPI       3310  GCAGCTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI         3041  GCGC                 GCG^C
Cfr10I       1471  GCCGGC               [GA]^CCGG[CT]
EcoRI          93  GAATTC               G^AATTC
FbaI         1766  TGATCA               T^GATCA
HaeII        3040  AGCGCC               [GA]GCGC^[CT]
HgaI         4057  GCGTC                GACGC(5/10)
HhaI         3041  GCGC                 GCG^C
HinP1I       3041  GCGC                 G^CGC
HincII        687  GTCAAC               GT[CT]^[GA]AC
HindII        687  GTCAAC               GT[CT]^[GA]AC
HindIII      1091  AAGCTT               A^AGCTT
KpnI         1956  GGTACC               GGTAC^C
NaeI         1471  GCCGGC               GCC^GGC
NciI         1516  CCGGG                CC^[GC]GG
NgoMIV       1471  GCCGGC               G^CCGGC
NheI         1159  GCTAGC               G^CTAGC
NspI          301  ACATGT               [GA]CATG^[CT]
PaeR7I        101  CTCGAG               C^TCGAG
PflMI        2016  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     2214  AACGTT               AA^CGTT
SapI         2039  GCTCTTC              GCTCTTC(1/4)
SexAI        1782  ACCTGGT              A^CC[AT]GGT
Van91I       2016  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          544  TCTAGA               T^CTAGA
XcmI         2630  CCACTGGGTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          101  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII        660  TCCGGA               T^CCGGA
             3094  TCCGGA              
AflIII        301  ACATGT               A^C[GA][CT]GT
             4049  ACGTGT              
AocI         1711  CCTAAGG              CC^T[ACGT]AGG
             3726  CCTGAGG             
Asp700I      1146  GAAAGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1598  GAACAGGTTC          
BbrPI        1453  CACGTG               CAC^GTG
             1798  CACGTG              
BlpI         2131  GCTCAGC              GC^T[ACGT]AGC
             2381  GCTCAGC             
Bpu1102I     2131  GCTCAGC              GC^T[ACGT]AGC
             2381  GCTCAGC             
BsaAI        1453  CACGTG               [CT]AC^GT[GA]
             1798  CACGTG              
BsaI          513  GAGACC               GGTCTC(1/5)
             1587  GGTCTC              
BseAI         660  TCCGGA               T^CCGGA
             3094  TCCGGA              
BspEI         660  TCCGGA               T^CCGGA
             3094  TCCGGA              
BstXI        2231  CCACTAATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2607  CCAGACTTCTGG        
Bsu36I       1711  CCTAAGG              CC^T[ACGT]AGG
             3726  CCTGAGG             
CelII        2131  GCTCAGC              GC^T[ACGT]AGC
             2381  GCTCAGC             
DraII         718  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             3833  AGGACCT             
Eco81I       1711  CCTAAGG              CC^T[ACGT]AGG
             3726  CCTGAGG             
EcoO109I      718  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             3833  AGGACCT             
FauI         1804  CCCGC                CCCGC(4/6)
             1836  CCCGC               
MroI          660  TCCGGA               T^CCGGA
             3094  TCCGGA              
NcoI         1263  CCATGG               C^CATGG
             2742  CCATGG              
PmaCI        1453  CACGTG               CAC^GTG
             1798  CACGTG              
PmlI         1453  CACGTG               CAC^GTG
             1798  CACGTG              
PpuMI         718  GGGTCCC              [GA]G^G[AT]CC[CT]
             3833  AGGACCT             
ScaI         2506  AGTACT               AGT^ACT
             3542  AGTACT              
SspI         4186  AATATT               AAT^ATT
             4248  AATATT              
XmnI         1146  GAAAGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1598  GAACAGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        AvaI        
AvaII       BalI        BanI        BbsI        BbvI        
BciVI       BfaI        BmyI        BpmI        BpuAI       
BsaBI       BsaJI       BsaWI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoNI       EcoRII      EcoRV       EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MfeI        
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PshBI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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