Restriction map for pF1KSDA1194
 (Linear) (Six-base) MAPSORT of: KIAA1194.seq  Check: 6538  from: 1  to: 1671



KIAA1194.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AccI GT'mk_AC

Cuts at:      0      43    1671 
   Size:         43    1628
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     697     771     959    1329    1671 
   Size:        185     512      74     188     370     342

  Fragments arranged by size: 

                 512     370     342     188     185      74
 
AflII C'TTAA_G

Cuts at:      0     126    1671 
   Size:        126    1545
 
AflIII A'CryG_T

Cuts at:      0     834    1671 
   Size:        834     837
 
AlwNI CAG_nnn'CTG

Cuts at:      0     478    1255    1562    1671 
   Size:        478     777     307     109

  Fragments arranged by size: 

                 777     478     307     109
 
ApaI G_GGCC'C

Cuts at:      0    1522    1671 
   Size:       1522     149
 
ApoI r'AATT_y

Cuts at:      0     194     254     871     901     924    1201    1671 
   Size:        194      60     617      30      23     277     470

  Fragments arranged by size: 

                 617     470     277     194      60      30      23
 
BanII G_rGCy'C

Cuts at:      0      23     305    1522    1619    1671 
   Size:         23     282    1217      97      52

  Fragments arranged by size: 

                1217     282      97      52      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      47     703    1671 
   Size:         47     656     968

  Fragments arranged by size: 

                 968     656      47
 
BbvCI CC'TCA_GC

Cuts at:      0    1493    1671 
   Size:       1493     178
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1002    1036    1671 
   Size:       1002      34     635

  Fragments arranged by size: 

                1002     635      34
 
BfrBI ATG'CAT

Cuts at:      0    1086    1671 
   Size:       1086     585
 
BglII A'GATC_T

Cuts at:      0    1233    1671 
   Size:       1233     438
 
Bme1580I G_kGCm'C

Cuts at:      0    1522    1671 
   Size:       1522     149
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      99     657    1671 
   Size:         99     558    1014

  Fragments arranged by size: 

                1014     558      99
 
BmtI G_CTAG'C

Cuts at:      0     916    1671 
   Size:        916     755
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1633    1671 
   Size:       1633      38
 
Bpu10I CC'TnA_GC

Cuts at:      0    1493    1671 
   Size:       1493     178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     705    1671 
   Size:        705     966
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1306    1336    1671 
   Size:       1306      30     335

  Fragments arranged by size: 

                1306     335      30
 
BseYI C'CCAG_C

Cuts at:      0     345    1671 
   Size:        345    1326
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1250    1671 
   Size:       1250     421
 
BsiHKAI G_wGCw'C

Cuts at:      0     305    1279    1619    1671 
   Size:        305     974     340      52

  Fragments arranged by size: 

                 974     340     305      52
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     305    1279    1522    1619    1671 
   Size:         23     282     974     243      97      52

  Fragments arranged by size: 

                 974     282     243      97      52      23
 
BspHI T'CATG_A

Cuts at:      0     959    1671 
   Size:        959     712
 
BsrDI GCAATG_nn'

Cuts at:      0     206     699    1671 
   Size:        206     493     972

  Fragments arranged by size: 

                 972     493     206
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     518    1194    1660    1671 
   Size:        518     676     466      11

  Fragments arranged by size: 

                 676     518     466      11
 
BstYI r'GATC_y

Cuts at:      0    1233    1671 
   Size:       1233     438
 
BstZ17I GTA'TAC

Cuts at:      0      44    1671 
   Size:         44    1627
 
Bsu36I CC'TnA_GG

Cuts at:      0    1506    1671 
   Size:       1506     165
 
BtgI C'CryG_G

Cuts at:      0     917    1671 
   Size:        917     754
 
ClaI AT'CG_AT

Cuts at:      0    1012    1671 
   Size:       1012     659
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1069    1104    1671 
   Size:       1069      35     567

  Fragments arranged by size: 

                1069     567      35
 
DraI TTT'AAA

Cuts at:      0     311    1671 
   Size:        311    1360
 
EcoICRI GAG'CTC

Cuts at:      0     303    1617    1671 
   Size:        303    1314      54

  Fragments arranged by size: 

                1314     303      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     697     771     959    1329    1633    1671 
   Size:        185     512      74     188     370     304      38

  Fragments arranged by size: 

                 512     370     304     188     185      74      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     397     418    1671 
   Size:        397      21    1253

  Fragments arranged by size: 

                1253     397      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1092    1519    1671 
   Size:       1092     427     152

  Fragments arranged by size: 

                1092     427     152
 
EcoRI G'AATT_C

Cuts at:      0     194    1671 
   Size:        194    1477
 
EcoRV GAT'ATC

Cuts at:      0     702    1671 
   Size:        702     969
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     152    1671 
   Size:        152    1519
 
HaeII r_GCGC'y

Cuts at:      0     643    1671 
   Size:        643    1028
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     229     261     454     486    1153    1185    1579    1611 
   Size:        229      32     193      32     667      32     394      32

Cuts at:   1611    1671 
   Size:         60

  Fragments arranged by size: 

                 667     394     229     193      60      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1644    1671 
   Size:       1644      27
 
HindIII A'AGCT_T

Cuts at:      0     123     214    1671 
   Size:        123      91    1457

  Fragments arranged by size: 

                1457     123      91
 
MslI CAynn'nnrTG

Cuts at:      0     138     173     839    1671 
   Size:        138      35     666     832

  Fragments arranged by size: 

                 832     666     138      35
 
MspA1I CmG'CkG

Cuts at:      0    1349    1497    1671 
   Size:       1349     148     174

  Fragments arranged by size: 

                1349     174     148
 
NcoI C'CATG_G

Cuts at:      0     917    1671 
   Size:        917     754
 
NheI G'CTAG_C

Cuts at:      0     912    1671 
   Size:        912     759
 
NsiI A_TGCA'T

Cuts at:      0    1088    1671 
   Size:       1088     583
 
NspI r_CATG'y

Cuts at:      0     838    1086    1671 
   Size:        838     248     585

  Fragments arranged by size: 

                 838     585     248
 
PciI A'CATG_T

Cuts at:      0     834    1671 
   Size:        834     837
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1042    1255    1539    1671 
   Size:       1042     213     284     132

  Fragments arranged by size: 

                1042     284     213     132
 
PpuMI rG'GwC_Cy

Cuts at:      0    1092    1671 
   Size:       1092     579
 
PspOMI G'GGCC_C

Cuts at:      0    1518    1671 
   Size:       1518     153
 
PvuII CAG'CTG

Cuts at:      0    1349    1497    1671 
   Size:       1349     148     174

  Fragments arranged by size: 

                1349     174     148
 
SacI G_AGCT'C

Cuts at:      0     305    1619    1671 
   Size:        305    1314      52

  Fragments arranged by size: 

                1314     305      52
 
SanDI GG'GwC_CC

Cuts at:      0    1092    1671 
   Size:       1092     579
 
ScaI AGT'ACT

Cuts at:      0     989    1671 
   Size:        989     682
 
SfcI C'TryA_G

Cuts at:      0     654    1671 
   Size:        654    1017
 
SmlI C'TyrA_G

Cuts at:      0     126     684    1671 
   Size:        126     558     987

  Fragments arranged by size: 

                 987     558     126
 
SspI AAT'ATT

Cuts at:      0     854    1270    1671 
   Size:        854     416     401

  Fragments arranged by size: 

                 854     416     401
 
StuI AGG'CCT

Cuts at:      0     377    1671 
   Size:        377    1294
 
StyI C'CwwG_G

Cuts at:      0     511     917    1671 
   Size:        511     406     754

  Fragments arranged by size: 

                 754     511     406
 
SwaI ATTT'AAAT

Cuts at:      0     311    1671 
   Size:        311    1360
 
TatI w'GTAC_w

Cuts at:      0     987    1671 
   Size:        987     684
 
Tth111I GACn'n_nGTC

Cuts at:      0     813    1671 
   Size:        813     858
 
XbaI T'CTAG_A

Cuts at:      0     799    1671 
   Size:        799     872
 
XmnI GAAnn'nnTTC

Cuts at:      0     198    1671 
   Size:        198    1473
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII    AlwNI     ApaI     ApoI    BanII
     BbsI    BbvCI     BcgI    BfrBI    BglII Bme1580I     BmrI     BmtI
     BpmI   Bpu10I    BpuEI    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrDI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I   HincII  HindIII     MslI   MspA1I     NcoI
     NheI     NsiI     NspI     PciI    PflMI    PpuMI   PspOMI    PvuII
     SacI    SanDI     ScaI     SfcI     SmlI     SspI     StuI     StyI
     SwaI     TatI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BciVI     BclI     BglI     BlpI
    BmgBI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BtgZI     BtsI
   DraIII     DrdI     EaeI     EagI     EarI     EciI     FseI     FspI
    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI    TaqII     TstI     XcmI     XhoI     XmaI     ZraI
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