Restriction map for pF1KSDA1158
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaWI       BseAI       BsiEI       BsiWI       BsmI        
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KspI        
MaeI        MfeI        MflI        MroI        MseI        
MspA1I      MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        SspI        StuI        
StyI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     XbaI        XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I        630  GGTACC               G^GTACC
AcyI          269  GGCGCC               G[GA]^CG[CT]C
AluI          125  AGCT                 AG^CT
AlwI          420  GATCC                GGATC(4/5)
AlwNI          57  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       630  GGTACC               G^GTACC
AspHI         319  GTGCTC               G[AT]GC[AT]^C
AvaII         294  GGACC                G^G[AT]CC
BalI          237  TGGCCA               TGG^CCA
BanII         255  GAGCCC               G[GA]GC[CT]^C
BbeI          269  GGCGCC               GGCGC^C
BglI          559  GCCGAAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          446  CTGGAG               CTGGAG(16/14)
BsaBI         483  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         269  GGCGCC               G[GA]^CG[CT]C
BsgI          106  GTGCAG               GTGCAG(16/14)
BsiHKAI       319  GTGCTC               G[AT]GC[AT]^C
BsmBI          94  GAGACG               CGTCTC(1/5)
BsmFI         366  GTCCC                GGGAC(10/14)
Bsp1407I      485  TGTACA               T^GTACA
BspHI         479  TCATGA               T^CATGA
BsrDI         359  GCAATG               GCAATG(2/0)
BsrGI         485  TGTACA               T^GTACA
BssSI          37  CTCGTG               CACGAG(-5/-1)
BstAPI        134  GCATCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII         420  GATC                 ^GATC
DraII         293  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        507  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          170  CCACGG               C^C[GA][CT]GG
EaeI          237  TGGCCA               [CT]^GGCC[GA]
EcoO109I      293  AGGACCT              [GA]G^G[ACGT]CC[CT]
HaeII         269  GGCGCC               [GA]GCGC^[CT]
HapII         369  CCGG                 C^CGG
Hin1I         269  GGCGCC               G[GA]^CG[CT]C
HpaII         369  CCGG                 C^CGG
KasI          269  GGCGCC               G^GCGCC
KpnI          630  GGTACC               GGTAC^C
MamI          483  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          420  GATC                 ^GATC
MluI          581  ACGCGT               A^CGCGT
MluNI         237  TGGCCA               TGG^CCA
MscI          237  TGGCCA               TGG^CCA
MspI          369  CCGG                 C^CGG
NarI          269  GGCGCC               GG^CGCC
NdeII         420  GATC                 ^GATC
PflMI         243  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         293  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          479  TCATGA               T^CATGA
Sau3AI        420  GATC                 ^GATC
SbfI          297  CCTGCAGG             CCTGCA^GG
SfoI          269  GGCGCC               GGC^GCC
SmaI          368  CCCGGG               CCC^GGG
Sse8387I      297  CCTGCAGG             CCTGCA^GG
SspBI         485  TGTACA               T^GTACA
TaqI          524  TCGA                 T^CGA
TthHB8I       524  TCGA                 T^CGA
Van91I        243  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          167  CCACCACGGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         368  CCCGGG               C^CCGGG
XmaI          368  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccII         413  CGCG                 CG^CG
              582  CGCG                
AfaI          486  GTAC                 GT^AC
              631  GTAC                
AflIII        305  ACGTGT               A^C[GA][CT]GT
              581  ACGCGT              
AvaI          196  CCCGAG               C^[CT]CG[GA]G
              368  CCCGGG              
BanI          269  GGCGCC               G^G[CT][GA]CC
              630  GGTACC              
BbsI          405  GAAGAC               GAAGAC(2/6)
              499  GTCTTC              
BcnI          368  CCCGG                CC^[GC]GG
              369  CCGGG               
BpuAI         405  GAAGAC               GAAGAC(2/6)
              499  GTCTTC              
BsaI          468  GGTCTC               GGTCTC(1/5)
              549  GGTCTC              
BseRI         154  GAGGAG               GAGGAG(10/8)
              439  GAGGAG              
BsoBI         196  CCCGAG               C^[CT]CG[GA]G
              368  CCCGGG              
BspMI         296  ACCTGC               ACCTGC(4/8)
              355  ACCTGC              
BsrI           50  ACTGG                ACTGG(1/-1)
              634  CCAGT               
BstF5I        489  CATCC                GGATG(2/0)
              600  CATCC               
BstNI          27  CCTGG                CC^[AT]GG
              243  CCAGG               
BstUI         413  CGCG                 CG^CG
              582  CGCG                
BstXI         167  CCACCACGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              170  CCACGGTGGTGG        
Cac8I           3  GCCTGC               GC[ACGT]^[ACGT]GC
              272  GCCTGC              
EarI          147  GAAGAG               CTCTTC(1/4)
              562  GAAGAG              
EcoRII         27  CCTGG                ^CC[AT]GG
              243  CCAGG               
FokI          489  CATCC                GGATG(9/13)
              600  CATCC               
HgaI          411  GACGC                GACGC(5/10)
              583  GCGTC               
HinfI         340  GACTC                G^A[ACGT]TC
              431  GAGTC               
Ksp632I       147  GAAGAG               CTCTTC(1/4)
              562  GAAGAG              
MaeII         305  ACGT                 A^CGT
              326  ACGT                
MaeIII        328  GTCAC                ^GT[ACGT]AC
              433  GTCAC               
MvaI           27  CCTGG                CC^[AT]GG
              243  CCAGG               
MvnI          413  CGCG                 CG^CG
              582  CGCG                
MwoI          134  GCATCTCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              559  GCCGAAGAGGC         
NciI          368  CCCGG                CC^[GC]GG
              369  CCGGG               
NlaIV         269  GGCGCC               GG[ACGT]^[ACGT]CC
              630  GGTACC              
PleI          340  GACTC                GAGTC(4/5)
              431  GAGTC               
PstI          274  CTGCAG               CTGCA^G
              298  CTGCAG              
RsaI          486  GTAC                 GT^AC
              631  GTAC                
SfaNI         134  GCATC                GCATC(5/9)
              390  GCATC               
Tsp45I        328  GTCAC                ^GT[GC]AC
              433  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        BbvI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       CfoI        
Cfr13I      DdeI        Fnu4HI      HaeIII      HhaI        
HinP1I      HphI        ItaI        MboII       MnlI        
MslI        NlaIII      Sau96I      ScrFI       SfcI        
TseI        TspRI       
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