Restriction map for pF1KSDA1158
 (Linear) (Six-base) MAPSORT of: KIAA1158.seq  Check: 911  from: 1  to: 645



KIAA1158.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     364     645 
   Size:        364     281
 
Acc65I G'GTAC_C

Cuts at:      0     630     645 
   Size:        630      15
 
AflIII A'CryG_T

Cuts at:      0     305     581     645 
   Size:        305     276      64

  Fragments arranged by size: 

                 305     276      64
 
AleI CACnn'nnGTG

Cuts at:      0     172     175     463     645 
   Size:        172       3     288     182

  Fragments arranged by size: 

                 288     182     172       3
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     313     645 
   Size:        313     332
 
AlwNI CAG_nnn'CTG

Cuts at:      0      62     645 
   Size:         62     583
 
AvaI C'yCGr_G

Cuts at:      0     196     368     645 
   Size:        196     172     277

  Fragments arranged by size: 

                 277     196     172
 
BanI G'GyrC_C

Cuts at:      0     269     630     645 
   Size:        269     361      15

  Fragments arranged by size: 

                 361     269      15
 
BanII G_rGCy'C

Cuts at:      0     259     645 
   Size:        259     386
 
BbeI G_GCGC'C

Cuts at:      0     273     645 
   Size:        273     372
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     412     492     645 
   Size:        412      80     153

  Fragments arranged by size: 

                 412     153      80
 
BglI GCCn_nnn'nGGC

Cuts at:      0     565     645 
   Size:        565      80
 
Bme1580I G_kGCm'C

Cuts at:      0      89     645 
   Size:         89     556
 
BmgBI CAC'GTC

Cuts at:      0     306     645 
   Size:        306     339
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      60     645 
   Size:         60     585
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     645 
   Size:        467     178
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     474     555     645 
   Size:        474      81      90

  Fragments arranged by size: 

                 474      90      81
 
BsaBI GATnn'nnATC

Cuts at:      0     487     645 
   Size:        487     158
 
BsaHI Gr'CG_yC

Cuts at:      0     270     645 
   Size:        270     375
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     364     394     645 
   Size:        364      30     251

  Fragments arranged by size: 

                 364     251      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     169     454     645 
   Size:        169     285     191

  Fragments arranged by size: 

                 285     191     169
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     645 
   Size:        127     518
 
BsiHKAI G_wGCw'C

Cuts at:      0     323     645 
   Size:        323     322
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      88     645 
   Size:         88     557
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     259     323     645 
   Size:         89     170      64     322

  Fragments arranged by size: 

                 322     170      89      64
 
BspHI T'CATG_A

Cuts at:      0     479     645 
   Size:        479     166
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     305     364     645 
   Size:        305      59     281

  Fragments arranged by size: 

                 305     281      59
 
BsrDI GCAATG_nn'

Cuts at:      0     366     645 
   Size:        366     279
 
BsrGI T'GTAC_A

Cuts at:      0     485     645 
   Size:        485     160
 
BssSI C'ACGA_G

Cuts at:      0      37     645 
   Size:         37     608
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     140     645 
   Size:        140     505
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     174     177     645 
   Size:        174       3     468

  Fragments arranged by size: 

                 468     174       3
 
BtgI C'CryG_G

Cuts at:      0     170     645 
   Size:        170     475
 
BtsI GCAGTG_nn'

Cuts at:      0     283     645 
   Size:        283     362
 
DraIII CAC_nnn'GTG

Cuts at:      0     512     645 
   Size:        512     133
 
EaeI y'GGCC_r

Cuts at:      0     237     645 
   Size:        237     408
 
EarI CTCTTCn'nnn_

Cuts at:      0     142     557     645 
   Size:        142     415      88

  Fragments arranged by size: 

                 415     142      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     645 
   Size:        467     178
 
EcoO109I rG'GnC_Cy

Cuts at:      0     294     645 
   Size:        294     351
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     232     645 
   Size:        232     413
 
HaeII r_GCGC'y

Cuts at:      0     273     645 
   Size:        273     372
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     645 
   Size:        445      32     168

  Fragments arranged by size: 

                 445     168      32
 
KasI G'GCGC_C

Cuts at:      0     269     645 
   Size:        269     376
 
KpnI G_GTAC'C

Cuts at:      0     634     645 
   Size:        634      11
 
MluI A'CGCG_T

Cuts at:      0     581     645 
   Size:        581      64
 
MscI TGG'CCA

Cuts at:      0     239     645 
   Size:        239     406
 
MslI CAynn'nnrTG

Cuts at:      0     172     175     316     463     526     645 
   Size:        172       3     141     147      63     119

  Fragments arranged by size: 

                 172     147     141     119      63       3
 
NarI GG'CG_CC

Cuts at:      0     270     645 
   Size:        270     375
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     249     645 
   Size:        249     396
 
PpuMI rG'GwC_Cy

Cuts at:      0     294     645 
   Size:        294     351
 
PstI C_TGCA'G

Cuts at:      0     278     302     645 
   Size:        278      24     343

  Fragments arranged by size: 

                 343     278      24
 
SbfI CC_TGCA'GG

Cuts at:      0     302     645 
   Size:        302     343
 
SfcI C'TryA_G

Cuts at:      0     189     274     298     540     645 
   Size:        189      85      24     242     105

  Fragments arranged by size: 

                 242     189     105      85      24
 
SfoI GGC'GCC

Cuts at:      0     271     645 
   Size:        271     374
 
SmaI CCC'GGG

Cuts at:      0     370     645 
   Size:        370     275
 
TatI w'GTAC_w

Cuts at:      0     485     645 
   Size:        485     160
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     174     645 
   Size:        174     471
 
XmaI C'CCGG_G

Cuts at:      0     368     645 
   Size:        368     277
 

 Enzymes that do cut:

     AarI   Acc65I   AflIII     AleI     AlfI    AlwNI     AvaI     BanI
    BanII     BbeI     BbsI     BglI Bme1580I    BmgBI     BmrI     BpmI
     BsaI    BsaBI    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstXI
     BtgI     BtsI   DraIII     EaeI     EarI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I     KasI     KpnI     MluI     MscI     MslI     NarI
    PflMI    PpuMI     PstI     SbfI     SfcI     SfoI     SmaI     TatI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaWI    BseYI
    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrFI   BssHII    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MmeI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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