Restriction map for pF1KSDA1142
 (Linear) (Six-base) MAPSORT of: KIAA1142.seq  Check: 6913  from: 1  to: 1314



KIAA1142.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AccI GT'mk_AC

Cuts at:      0     161    1028    1314 
   Size:        161     867     286

  Fragments arranged by size: 

                 867     286     161
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1165    1195    1261    1314 
   Size:       1165      30      66      53

  Fragments arranged by size: 

                1165      66      53      30
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1035    1314 
   Size:       1035     279
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     961    1314 
   Size:        961     353
 
AlwNI CAG_nnn'CTG

Cuts at:      0     123     822    1314 
   Size:        123     699     492

  Fragments arranged by size: 

                 699     492     123
 
ApaI G_GGCC'C

Cuts at:      0     198     398    1314 
   Size:        198     200     916

  Fragments arranged by size: 

                 916     200     198
 
ApaLI G'TGCA_C

Cuts at:      0      61    1314 
   Size:         61    1253
 
AvaI C'yCGr_G

Cuts at:      0     190     293     299     567    1314 
   Size:        190     103       6     268     747

  Fragments arranged by size: 

                 747     268     190     103       6
 
BanI G'GyrC_C

Cuts at:      0     304     742    1314 
   Size:        304     438     572

  Fragments arranged by size: 

                 572     438     304
 
BanII G_rGCy'C

Cuts at:      0     198     300     347     398     532     713     998    1079 
   Size:        198     102      47      51     134     181     285      81

Cuts at:   1079    1314 
   Size:        235

  Fragments arranged by size: 

                 285     235     198     181     134     102      81      51
                  47
 
BbeI G_GCGC'C

Cuts at:      0     746    1314 
   Size:        746     568
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     447    1314 
   Size:        447     867
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1219    1314 
   Size:       1219      95
 
BglII A'GATC_T

Cuts at:      0      29    1314 
   Size:         29    1285
 
Bme1580I G_kGCm'C

Cuts at:      0      65     198     309     398     950    1289    1314 
   Size:         65     133     111      89     552     339      25

  Fragments arranged by size: 

                 552     339     133     111      89      65      25
 
BmgBI CAC'GTC

Cuts at:      0     219    1314 
   Size:        219    1095
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     255    1314 
   Size:        255    1059
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     763    1017    1314 
   Size:        763     254     297

  Fragments arranged by size: 

                 763     297     254
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     344    1314 
   Size:        338       6     970

  Fragments arranged by size: 

                 970     338       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1199    1314 
   Size:       1199     115
 
BsaHI Gr'CG_yC

Cuts at:      0     743     845    1314 
   Size:        743     102     469

  Fragments arranged by size: 

                 743     469     102
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     478     508    1314 
   Size:        478      30     806

  Fragments arranged by size: 

                 806     478      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145     258     261     264     267     796    1314 
   Size:        145     113       3       3       3     529     518

  Fragments arranged by size: 

                 529     518     145     113       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     310     324     347    1041    1214    1314 
   Size:        310      14      23     694     173     100

  Fragments arranged by size: 

                 694     310     173     100      23      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351    1138    1314 
   Size:        351     787     176

  Fragments arranged by size: 

                 787     351     176
 
BsiEI CG_ry'CG

Cuts at:      0     797    1314 
   Size:        797     517
 
BsiHKAI G_wGCw'C

Cuts at:      0      56      65     713     833     998    1314 
   Size:         56       9     648     120     165     316

  Fragments arranged by size: 

                 648     316     165     120      56       9
 
Bsp1286I G_dGCh'C

Cuts at:      0      56      65     198     300     309     347     398     532 
   Size:         56       9     133     102       9      38      51     134

Cuts at:    532     713     833     950     998    1079    1289    1314 
   Size:        181     120     117      48      81     210      25

  Fragments arranged by size: 

                 210     181     134     133     120     117     102      81
                  56      51      48      38      25       9       9
 
BspHI T'CATG_A

Cuts at:      0     213     647    1314 
   Size:        213     434     667

  Fragments arranged by size: 

                 667     434     213
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     608    1139    1160    1314 
   Size:        608     531      21     154

  Fragments arranged by size: 

                 608     531     154      21
 
BsrBI CCG'CTC

Cuts at:      0     410     494    1314 
   Size:        410      84     820

  Fragments arranged by size: 

                 820     410      84
 
BsrFI r'CCGG_y

Cuts at:      0     143    1314 
   Size:        143    1171
 
BsrGI T'GTAC_A

Cuts at:      0     683    1314 
   Size:        683     631
 
BssSI C'ACGA_G

Cuts at:      0      82     664    1314 
   Size:         82     582     650

  Fragments arranged by size: 

                 650     582      82
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      60     545    1314 
   Size:         60     485     769

  Fragments arranged by size: 

                 769     485      60
 
BstYI r'GATC_y

Cuts at:      0      29    1314 
   Size:         29    1285
 
BtgI C'CryG_G

Cuts at:      0     533    1223    1314 
   Size:        533     690      91

  Fragments arranged by size: 

                 690     533      91
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     534    1314 
   Size:        534     780
 
BtsI GCAGTG_nn'

Cuts at:      0     815    1314 
   Size:        815     499
 
DraIII CAC_nnn'GTG

Cuts at:      0      60    1161    1314 
   Size:         60    1101     153

  Fragments arranged by size: 

                1101     153      60
 
EaeI y'GGCC_r

Cuts at:      0     251     581     794    1256    1314 
   Size:        251     330     213     462      58

  Fragments arranged by size: 

                 462     330     251     213      58
 
EagI C'GGCC_G

Cuts at:      0     794    1314 
   Size:        794     520
 
EarI CTCTTCn'nnn_

Cuts at:      0       4     634    1314 
   Size:          4     630     680

  Fragments arranged by size: 

                 680     630       4
 
EcoICRI GAG'CTC

Cuts at:      0     711     996    1314 
   Size:        711     285     318

  Fragments arranged by size: 

                 711     318     285
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     344    1165    1195    1261    1314 
   Size:        338       6     821      30      66      53

  Fragments arranged by size: 

                 821     338      66      53      30       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     127    1156    1258    1314 
   Size:        127    1029     102      56

  Fragments arranged by size: 

                1029     127     102      56
 
EcoO109I rG'GnC_Cy

Cuts at:      0     194     195     246     328     395     819    1314 
   Size:        194       1      51      82      67     424     495

  Fragments arranged by size: 

                 495     424     194      82      67      51       1
 
FspI TGC'GCA

Cuts at:      0     562     604    1314 
   Size:        562      42     710

  Fragments arranged by size: 

                 710     562      42
 
HaeII r_GCGC'y

Cuts at:      0     746    1314 
   Size:        746     568
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     478     510    1021    1053    1314 
   Size:        478      32     511      32     261

  Fragments arranged by size: 

                 511     478     261      32      32
 
HincII GTy'rAC

Cuts at:      0     162    1314 
   Size:        162    1152
 
KasI G'GCGC_C

Cuts at:      0     742    1314 
   Size:        742     572
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      68    1314 
   Size:         68    1246
 
MscI TGG'CCA

Cuts at:      0     253    1258    1314 
   Size:        253    1005      56

  Fragments arranged by size: 

                1005     253      56
 
MslI CAynn'nnrTG

Cuts at:      0     668     772    1114    1314 
   Size:        668     104     342     200

  Fragments arranged by size: 

                 668     342     200     104
 
MspA1I CmG'CkG

Cuts at:      0     241     351     420     468    1218    1314 
   Size:        241     110      69      48     750      96

  Fragments arranged by size: 

                 750     241     110      96      69      48
 
NaeI GCC'GGC

Cuts at:      0     145    1314 
   Size:        145    1169
 
NarI GG'CG_CC

Cuts at:      0     743    1314 
   Size:        743     571
 
NgoMIV G'CCGG_C

Cuts at:      0     143    1314 
   Size:        143    1171
 
PasI CC'CwG_GG

Cuts at:      0     840    1314 
   Size:        840     474
 
PfoI T'CCnGG_A

Cuts at:      0     731    1184    1314 
   Size:        731     453     130

  Fragments arranged by size: 

                 731     453     130
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108    1314 
   Size:         76      32    1206

  Fragments arranged by size: 

                1206      76      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     328    1314 
   Size:        328     986
 
PspOMI G'GGCC_C

Cuts at:      0     194     394    1314 
   Size:        194     200     920

  Fragments arranged by size: 

                 920     200     194
 
PstI C_TGCA'G

Cuts at:      0     467     818    1314 
   Size:        467     351     496

  Fragments arranged by size: 

                 496     467     351
 
PvuII CAG'CTG

Cuts at:      0     351     468    1314 
   Size:        351     117     846

  Fragments arranged by size: 

                 846     351     117
 
SacI G_AGCT'C

Cuts at:      0     713     998    1314 
   Size:        713     285     316

  Fragments arranged by size: 

                 713     316     285
 
SalI G'TCGA_C

Cuts at:      0     160    1314 
   Size:        160    1154
 
SanDI GG'GwC_CC

Cuts at:      0     328    1314 
   Size:        328     986
 
SapI GCTCTTCn'nnn_

Cuts at:      0     634    1314 
   Size:        634     680
 
SexAI A'CCwGG_T

Cuts at:      0     695    1314 
   Size:        695     619
 
SfcI C'TryA_G

Cuts at:      0     463     814    1314 
   Size:        463     351     500

  Fragments arranged by size: 

                 500     463     351
 
SfoI GGC'GCC

Cuts at:      0     744    1314 
   Size:        744     570
 
SmaI CCC'GGG

Cuts at:      0     192    1314 
   Size:        192    1122
 
StyI C'CwwG_G

Cuts at:      0     200    1259    1314 
   Size:        200    1059      55

  Fragments arranged by size: 

                1059     200      55
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     705     778    1262    1314 
   Size:        705      73     484      52

  Fragments arranged by size: 

                 705     484      73      52
 
TatI w'GTAC_w

Cuts at:      0     683    1314 
   Size:        683     631
 
Tth111I GACn'n_nGTC

Cuts at:      0     757    1314 
   Size:        757     557
 
XmaI C'CCGG_G

Cuts at:      0     190    1314 
   Size:        190    1124
 
XmnI GAAnn'nnTTC

Cuts at:      0    1180    1314 
   Size:       1180     134
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AlfI    AlwNI     ApaI    ApaLI     AvaI
     BanI    BanII     BbeI    BciVI     BglI    BglII Bme1580I    BmgBI
     BmrI     BplI     BpmI     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
    BsrGI    BssSI   BstAPI    BstYI     BtgI    BtgZI     BtsI   DraIII
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI
    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     PasI     PfoI     PpiI    PpuMI   PspOMI
     PstI    PvuII     SacI     SalI    SanDI     SapI    SexAI     SfcI
     SfoI     SmaI     StyI    TaqII     TatI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BbvCI     BcgI     BclI    BfrBI     BlpI     BmtI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI
    BspEI    BsrDI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     DrdI     EciI    EcoRI    EcoRV     FalI
     FseI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
     XbaI     XcmI     XhoI     ZraI
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