Restriction map for
pF1KSDA1138
(Circular) (Six-base) MAPSORT of: pF1KSDA1138.seq Check: 6550 from: 1 to: 6802
pF1KSDA1138 6802 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 942 1287 942
Size: 345 6457
AatII G_ACGT'C
Cuts at: 3500 3500
Size: 6802
AccI GT'mk_AC
Cuts at: 3371 3838 3371
Size: 467 6335
Acc65I G'GTAC_C
Cuts at: 3816 3816
Size: 6802
AclI AA'CG_TT
Cuts at: 6642 6642
Size: 6802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1678 3062 3598 4625 5057 5806 6074 1678
Size: 1384 536 1027 432 749 268 2406
Fragments arranged by size:
2406 1384 1027 749 536 432 268
AfeI AGC'GCT
Cuts at: 2584 2757 3528 2584
Size: 173 771 5858
Fragments arranged by size:
5858 771 173
AflIII A'CryG_T
Cuts at: 3167 3219 3605 4152 5258 6235 3167
Size: 52 386 547 1106 977 3734
Fragments arranged by size:
3734 1106 977 547 386 52
AgeI A'CCGG_T
Cuts at: 5190 5190
Size: 6802
AleI CACnn'nnGTG
Cuts at: 1450 3379 3403 3598 3610 1450
Size: 1929 24 195 12 4642
Fragments arranged by size:
4642 1929 195 24 12
AlwNI CAG_nnn'CTG
Cuts at: 2433 3041 4105 5674 2433
Size: 608 1064 1569 3561
Fragments arranged by size:
3561 1569 1064 608
ApaI G_GGCC'C
Cuts at: 194 234 241 544 1275 1305 1378 1671 2220
Size: 40 7 303 731 30 73 293 549
Cuts at: 2220 194
Size: 4776
Fragments arranged by size:
4776 731 549 303 293 73 40 30
7
ApaLI G'TGCA_C
Cuts at: 3382 5572 3382
Size: 2190 4612
ApoI r'AATT_y
Cuts at: 3804 6155 6411 3804
Size: 2351 256 4195
Fragments arranged by size:
4195 2351 256
AscI GG'CGCG_CC
Cuts at: 245 245
Size: 6802
AseI AT'TA_AT
Cuts at: 20 20
Size: 6802
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6802
AvaI C'yCGr_G
Cuts at: 184 233 875 902 2095 2151 2870 2980 3297
Size: 49 642 27 1193 56 719 110 317
Cuts at: 3297 3820 184
Size: 523 3166
Fragments arranged by size:
3166 1193 719 642 523 317 110 56
49 27
BamHI G'GATC_C
Cuts at: 3825 3825
Size: 6802
BanI G'GyrC_C
Cuts at: 180 221 267 343 507 715 854 1633 1728
Size: 41 46 76 164 208 139 779 95
Cuts at: 1728 2169 2365 3055 3733 3816 4230 4478 4513
Size: 441 196 690 678 83 414 248 35
Cuts at: 4513 180
Size: 2469
Fragments arranged by size:
2469 779 690 678 441 414 248 208
196 164 139 95 83 76 46 41
35
BanII G_rGCy'C
Cuts at: 194 234 241 544 910 926 1028 1275 1305
Size: 40 7 303 366 16 102 247 30
Cuts at: 1305 1378 1504 1671 2150 2220 3281 3814 4844
Size: 73 126 167 479 70 1061 533 1030
Cuts at: 4844 194
Size: 2152
Fragments arranged by size:
2152 1061 1030 533 479 366 303 247
167 126 102 73 70 40 30 16
7
BbeI G_GCGC'C
Cuts at: 184 271 511 1637 3737 4234 4482 184
Size: 87 240 1126 2100 497 248 2504
Fragments arranged by size:
2504 2100 1126 497 248 240 87
BbsI GAAGACnn'nnnn_
Cuts at: 3268 3776 3268
Size: 508 6294
BbvCI CC'TCA_GC
Cuts at: 859 1762 2770 3005 859
Size: 903 1008 235 4656
Fragments arranged by size:
4656 1008 903 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2232 2261 2266 2295 2232
Size: 29 5 29 6739
Fragments arranged by size:
6739 29 29 5
BciVI GTATCCnnnnn_n'
Cuts at: 4698 5461 6802 4698
Size: 763 1341 4698
Fragments arranged by size:
4698 1341 763
BclI T'GATC_A
Cuts at: 4320 4320
Size: 6802
BglI GCCn_nnn'nGGC
Cuts at: 189 198 412 756 1482 1491 1830 2224 2269
Size: 9 214 344 726 9 339 394 45
Cuts at: 2269 3053 3251 189
Size: 784 198 3740
Fragments arranged by size:
3740 784 726 394 344 339 214 198
45 9 9
BglII A'GATC_T
Cuts at: 4315 6354 4315
Size: 2039 4763
BlpI GC'TnA_GC
Cuts at: 2851 3514 3912 2851
Size: 663 398 5741
Fragments arranged by size:
5741 663 398
Bme1580I G_kGCm'C
Cuts at: 194 234 241 544 720 1275 1305 1378 1671
Size: 40 7 303 176 555 30 73 293
Cuts at: 1671 2174 2220 2765 3386 3713 4425 4518 5576
Size: 503 46 545 621 327 712 93 1058
Cuts at: 5576 194
Size: 1420
Fragments arranged by size:
1420 1058 712 621 555 545 503 327
303 293 176 93 73 46 40 30
7
BmgBI CAC'GTC
Cuts at: 1161 3381 3608 1161
Size: 2220 227 4355
Fragments arranged by size:
4355 2220 227
BmrI ACTGGGnnnn_n'
Cuts at: 770 3126 3156 4012 4093 4428 6574 770
Size: 2356 30 856 81 335 2146 998
Fragments arranged by size:
2356 2146 998 856 335 81 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 501 1653 5167 501
Size: 1152 3514 2136
Fragments arranged by size:
3514 2136 1152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 457 484 786 841 1126 1156 1540 1776
Size: 54 27 302 55 285 30 384 236
Cuts at: 1776 1881 1951 3367 3577 3702 403
Size: 105 70 1416 210 125 3503
Fragments arranged by size:
3503 1416 384 302 285 236 210 125
105 70 55 54 30 27
Bpu10I CC'TnA_GC
Cuts at: 308 859 1672 1762 2414 2489 2737 2770 3005
Size: 551 813 90 652 75 248 33 235
Cuts at: 3005 308
Size: 4105
Fragments arranged by size:
4105 813 652 551 248 235 90 75
33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1819 2599 2713 2926 3268 3976 4855 5349 5647
Size: 780 114 213 342 708 879 494 298
Cuts at: 5647 1819
Size: 2974
Fragments arranged by size:
2974 879 780 708 494 342 298 213
114
BsaI GGTCTCn'nnnn_
Cuts at: 1126 3151 6506 1126
Size: 2025 3355 1422
Fragments arranged by size:
3355 2025 1422
BsaAI yAC'GTr
Cuts at: 789 3222 3387 3792 4153 789
Size: 2433 165 405 361 3438
Fragments arranged by size:
3438 2433 405 361 165
BsaBI GATnn'nnATC
Cuts at: 6 4314 6353 6
Size: 4308 2039 455
Fragments arranged by size:
4308 2039 455
BsaHI Gr'CG_yC
Cuts at: 181 268 508 673 1634 1646 2461 3497 3728
Size: 87 240 165 961 12 815 1036 231
Cuts at: 3728 3734 3773 4231 4479 5183 6679 181
Size: 6 39 458 248 704 1496 304
Fragments arranged by size:
1496 1036 961 815 704 458 304 248
240 231 165 87 39 12 6
BsaWI w'CCGG_w
Cuts at: 3989 4510 5190 5464 5611 6022 3989
Size: 521 680 274 147 411 4769
Fragments arranged by size:
4769 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 820 850 921 951 1762 1774 1792 1804 1936
Size: 30 71 30 811 12 18 12 132
Cuts at: 1936 1966 3148 3178 820
Size: 30 1182 30 4444
Fragments arranged by size:
4444 1182 811 132 71 30 30 30
30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 1860 1863 1866 1869 1872 1875 1878 1981
Size: 1484 3 3 3 3 3 3 103
Cuts at: 1981 2074 2121 2169 2331 2620 2677 3022 3115
Size: 93 47 48 162 289 57 345 93
Cuts at: 3115 376
Size: 4063
Fragments arranged by size:
4063 1484 345 289 162 103 93 93
57 48 47 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 285 1182 1200 1285 1756 2219 2689 2752 3152
Size: 897 18 85 471 463 470 63 400
Cuts at: 3152 4225 4983 5562 6058 285
Size: 1073 758 579 496 1029
Fragments arranged by size:
1073 1029 897 758 579 496 471 470
463 400 85 63 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 3244 1171
Size: 2073 4729
BsiEI CG_ry'CG
Cuts at: 104 1148 1414 1498 1525 1532 4342 4388 5598
Size: 1044 266 84 27 7 2810 46 1210
Cuts at: 5598 104
Size: 1308
Fragments arranged by size:
2810 1308 1210 1044 266 84 46 27
7
BsiHKAI G_wGCw'C
Cuts at: 482 1579 3281 3386 3617 3814 4592 4782 5576
Size: 1097 1702 105 231 197 778 190 794
Cuts at: 5576 482
Size: 1708
Fragments arranged by size:
1708 1702 1097 794 778 231 197 190
105
BsmBI CGTCTCn'nnnn_
Cuts at: 460 1135 460
Size: 675 6127
Bsp1286I G_dGCh'C
Cuts at: 194 234 241 482 544 720 910 926 1028
Size: 40 7 241 62 176 190 16 102
Cuts at: 1028 1275 1305 1378 1504 1579 1671 2150 2174
Size: 247 30 73 126 75 92 479 24
Cuts at: 2174 2220 2765 3281 3386 3617 3713 3814 4425
Size: 46 545 516 105 231 96 101 611
Cuts at: 4425 4518 4592 4782 4844 5576 194
Size: 93 74 190 62 732 1420
Fragments arranged by size:
1420 732 611 545 516 479 247 241
231 190 190 176 126 105 102 101
96 93 92 75 74 73 62 62
46 40 30 24 16 7
BspEI T'CCGG_A
Cuts at: 6022 6022
Size: 6802
BspHI T'CATG_A
Cuts at: 5978 5978
Size: 6802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 942 1287 2219 2813 2930 3049 3850 4064 4366
Size: 345 932 594 117 119 801 214 302
Cuts at: 4366 4747 942
Size: 381 2997
Fragments arranged by size:
2997 932 801 594 381 345 302 214
119 117
BsrBI CCG'CTC
Cuts at: 50 485 885 1410 1608 1619 2976 5092 5146
Size: 435 400 525 198 11 1357 2116 54
Cuts at: 5146 6632 6797 50
Size: 1486 165 55
Fragments arranged by size:
2116 1486 1357 525 435 400 198 165
55 54 11
BsrDI GCAATG_nn'
Cuts at: 4712 4712
Size: 6802
BsrFI r'CCGG_y
Cuts at: 197 613 1481 1524 1531 2021 3187 3730 4798
Size: 416 868 43 7 490 1166 543 1068
Cuts at: 4798 4979 5190 197
Size: 181 211 1809
Fragments arranged by size:
1809 1166 1068 868 543 490 416 211
181 43 7
BsrGI T'GTAC_A
Cuts at: 2900 2900
Size: 6802
BssHII G'CGCG_C
Cuts at: 245 245
Size: 6802
BssSI C'ACGA_G
Cuts at: 2803 3067 5071 5431 2803
Size: 264 2004 360 4174
Fragments arranged by size:
4174 2004 360 264
BstAPI GCAn_nnn'nTGC
Cuts at: 3041 3222 4177 3041
Size: 181 955 5666
Fragments arranged by size:
5666 955 181
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6802
BstXI CCAn_nnnn'nTGG
Cuts at: 633 819 1452 633
Size: 186 633 5983
Fragments arranged by size:
5983 633 186
BstYI r'GATC_y
Cuts at: 1 1979 2111 3302 3825 4292 4315 4650 4896
Size: 1978 132 1191 523 467 23 335 246
Cuts at: 4896 5910 5996 6008 6354 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1978 1191 1014 523 467 449 346 335
246 132 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1461 2911 1461
Size: 1450 5352
BtgI C'CryG_G
Cuts at: 225 513 591 912 1007 1031 1961 2165 3596
Size: 288 78 321 95 24 930 204 1431
Cuts at: 3596 225
Size: 3431
Fragments arranged by size:
3431 1431 930 321 288 204 95 78
24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 986 4750 4931 5094 986
Size: 3764 181 163 2694
Fragments arranged by size:
3764 2694 181 163
BtsI GCAGTG_nn'
Cuts at: 429 924 1159 4043 429
Size: 495 235 2884 3188
Fragments arranged by size:
3188 2884 495 235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2763 2798 2763
Size: 35 6767
DraI TTT'AAA
Cuts at: 2525 3799 2525
Size: 1274 5528
DraIII CAC_nnn'GTG
Cuts at: 2808 3750 2808
Size: 942 5860
DrdI GACnn_nn'nnGTC
Cuts at: 3481 4506 5366 3481
Size: 1025 860 4917
Fragments arranged by size:
4917 1025 860
EaeI y'GGCC_r
Cuts at: 635 1034 1145 1411 1442 1454 1483 1495 1529
Size: 399 111 266 31 12 29 12 34
Cuts at: 1529 2384 2424 2714 2784 2828 3143 3231 3668
Size: 855 40 290 70 44 315 88 437
Cuts at: 3668 4385 4559 4950 4977 6324 635
Size: 717 174 391 27 1347 1113
Fragments arranged by size:
1347 1113 855 717 437 399 391 315
290 266 174 111 88 70 44 40
34 31 29 27 12 12
EagI C'GGCC_G
Cuts at: 1145 1411 1495 1529 4385 1145
Size: 266 84 34 2856 3562
Fragments arranged by size:
3562 2856 266 84 34
EarI CTCTTCn'nnn_
Cuts at: 1567 2042 2047 2098 2308 3007 3033 3081 3622
Size: 475 5 51 210 699 26 48 541
Cuts at: 3622 4823 5033 1567
Size: 1201 210 3336
Fragments arranged by size:
3336 1201 699 541 475 210 210 51
48 26 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 275 477 1924 2289 2566 5008 5320 5466 6612
Size: 202 1447 365 277 2442 312 146 1146
Cuts at: 6612 275
Size: 465
Fragments arranged by size:
2442 1447 1146 465 365 312 277 202
146
EcoICRI GAG'CTC
Cuts at: 3279 3812 3279
Size: 533 6269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 457 484 786 841 1126 1156 1540 1678
Size: 54 27 302 55 285 30 384 138
Cuts at: 1678 1776 1881 1951 3062 3367 3577 3598 3702
Size: 98 105 70 1111 305 210 21 104
Cuts at: 3702 4625 5057 5806 6074 403
Size: 923 432 749 268 1131
Fragments arranged by size:
1131 1111 923 749 432 384 305 302
285 268 210 138 105 104 98 70
55 54 30 27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 358 1933 2417 358
Size: 1575 484 4743
Fragments arranged by size:
4743 1575 484
EcoO109I rG'GnC_Cy
Cuts at: 190 191 230 237 238 495 1101 1167 1271
Size: 1 39 7 1 257 606 66 104
Cuts at: 1271 1272 1301 1374 1584 1668 2161 2256 2375
Size: 1 29 73 210 84 493 95 119
Cuts at: 2375 2742 2880 3038 3939 190
Size: 367 138 158 901 3053
Fragments arranged by size:
3053 901 606 493 367 257 210 158
138 119 104 95 84 73 66 39
29 7 1 1 1
EcoRI G'AATT_C
Cuts at: 3804 3804
Size: 6802
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2859 4819 2859
Size: 1960 4842
FspI TGC'GCA
Cuts at: 421 4581 421
Size: 4160 2642
HaeII r_GCGC'y
Cuts at: 184 271 450 511 1637 2586 2759 3246 3530
Size: 87 179 61 1126 949 173 487 284
Cuts at: 3530 3737 4234 4482 5506 6485 184
Size: 207 497 248 1024 979 501
Fragments arranged by size:
1126 1024 979 949 501 497 487 284
248 207 179 173 87 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 820 852 1762 1768 1774 1794 1800 1806 1936
Size: 32 910 6 6 20 6 6 130
Cuts at: 1936 1968 2145 2177 3674 3706 4642 4672 4674
Size: 32 177 32 1497 32 936 30 2
Cuts at: 4674 4704 820
Size: 30 2918
Fragments arranged by size:
2918 1497 936 910 177 130 32 32
32 32 30 30 20 6 6 6
6 2
HincII GTy'rAC
Cuts at: 399 3372 3839 399
Size: 2973 467 3362
Fragments arranged by size:
3362 2973 467
KasI G'GCGC_C
Cuts at: 180 267 507 1633 3733 4230 4478 180
Size: 87 240 1126 2100 497 248 2504
Fragments arranged by size:
2504 2100 1126 497 248 240 87
KpnI G_GTAC'C
Cuts at: 3820 3820
Size: 6802
MluI A'CGCG_T
Cuts at: 6235 6235
Size: 6802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 863 1805 1979 2429 2654 5473 5657 368
Size: 495 942 174 450 225 2819 184 1513
Fragments arranged by size:
2819 1513 942 495 450 225 184 174
MscI TGG'CCA
Cuts at: 1456 2426 4561 1456
Size: 970 2135 3697
Fragments arranged by size:
3697 2135 970
MslI CAynn'nnrTG
Cuts at: 1450 3379 3403 3598 3610 4789 4916 1450
Size: 1929 24 195 12 1179 127 3336
Fragments arranged by size:
3336 1929 1179 195 127 24 12
MspA1I CmG'CkG
Cuts at: 227 444 515 914 1289 1560 2167 2390 2433
Size: 217 71 399 375 271 607 223 43
Cuts at: 2433 3004 3050 3086 3911 4225 4585 5600 5845
Size: 571 46 36 825 314 360 1015 245
Cuts at: 5845 227
Size: 1184
Fragments arranged by size:
1184 1015 825 607 571 399 375 360
314 271 245 223 217 71 46 43
36
NaeI GCC'GGC
Cuts at: 199 615 1533 2023 3189 3732 4981 199
Size: 416 918 490 1166 543 1249 2020
Fragments arranged by size:
2020 1249 1166 918 543 490 416
NarI GG'CG_CC
Cuts at: 181 268 508 1634 3734 4231 4479 181
Size: 87 240 1126 2100 497 248 2504
Fragments arranged by size:
2504 2100 1126 497 248 240 87
NcoI C'CATG_G
Cuts at: 1961 1961
Size: 6802
NdeI CA'TA_TG
Cuts at: 3339 3339
Size: 6802
NgoMIV G'CCGG_C
Cuts at: 197 613 1531 2021 3187 3730 4979 197
Size: 416 918 490 1166 543 1249 2020
Fragments arranged by size:
2020 1249 1166 918 543 490 416
NruI TCG'CGA
Cuts at: 5163 5163
Size: 6802
NspI r_CATG'y
Cuts at: 3171 3522 3744 3853 4353 5262 3171
Size: 351 222 109 500 909 4711
Fragments arranged by size:
4711 909 500 351 222 109
PasI CC'CwG_GG
Cuts at: 348 1305 348
Size: 957 5845
PciI A'CATG_T
Cuts at: 3167 5258 3167
Size: 2091 4711
PflMI CCAn_nnn'nTGG
Cuts at: 2142 2808 3630 2142
Size: 666 822 5314
Fragments arranged by size:
5314 822 666
PfoI T'CCnGG_A
Cuts at: 1805 3092 3692 1805
Size: 1287 600 4915
Fragments arranged by size:
4915 1287 600
PmeI GTTT'AAAC
Cuts at: 3799 3799
Size: 6802
PmlI CAC'GTG
Cuts at: 3222 3387 3222
Size: 165 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5941 5973 5941
Size: 32 6770
PpuMI rG'GwC_Cy
Cuts at: 1167 2161 2375 2880 3038 1167
Size: 994 214 505 158 4931
Fragments arranged by size:
4931 994 505 214 158
PshAI GACnn'nnGTC
Cuts at: 686 3358 686
Size: 2672 4130
PspOMI G'GGCC_C
Cuts at: 190 230 237 540 1271 1301 1374 1667 2216
Size: 40 7 303 731 30 73 293 549
Cuts at: 2216 190
Size: 4776
Fragments arranged by size:
4776 731 549 303 293 73 40 30
7
PstI C_TGCA'G
Cuts at: 2255 2355 3037 3847 4532 2255
Size: 100 682 810 685 4525
Fragments arranged by size:
4525 810 685 682 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6802
PvuII CAG'CTG
Cuts at: 1289 1560 2433 3004 4225 4585 1289
Size: 271 873 571 1221 360 3506
Fragments arranged by size:
3506 1221 873 571 360 271
RsrII CG'GwC_CG
Cuts at: 4995 4995
Size: 6802
SacI G_AGCT'C
Cuts at: 3281 3814 3281
Size: 533 6269
SacII CC_GC'GG
Cuts at: 228 516 915 2168 228
Size: 288 399 1253 4862
Fragments arranged by size:
4862 1253 399 288
SalI G'TCGA_C
Cuts at: 3370 3837 3370
Size: 467 6335
SanDI GG'GwC_CC
Cuts at: 2161 2161
Size: 6802
SapI GCTCTTCn'nnn_
Cuts at: 1567 3622 4823 5033 1567
Size: 2055 1201 210 3336
Fragments arranged by size:
3336 2055 1201 210
SbfI CC_TGCA'GG
Cuts at: 3037 3847 3037
Size: 810 5992
ScaI AGT'ACT
Cuts at: 664 6319 664
Size: 5655 1147
SexAI A'CCwGG_T
Cuts at: 3439 3746 3439
Size: 307 6495
SfcI C'TryA_G
Cuts at: 33 576 2251 2351 3033 3843 4528 5523 5714
Size: 543 1675 100 682 810 685 995 191
Cuts at: 5714 33
Size: 1121
Fragments arranged by size:
1675 1121 995 810 685 682 543 191
100
SfiI GGCCn_nnn'nGGCC
Cuts at: 412 1482 412
Size: 1070 5732
SfoI GGC'GCC
Cuts at: 182 269 509 1635 3735 4232 4480 182
Size: 87 240 1126 2100 497 248 2504
Fragments arranged by size:
2504 2100 1126 497 248 240 87
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6802
SgrAI Cr'CCGG_yG
Cuts at: 3730 3730
Size: 6802
SmaI CCC'GGG
Cuts at: 186 235 877 904 2097 2872 2982 3299 3822
Size: 49 642 27 1193 775 110 317 523
Cuts at: 3822 186
Size: 3166
Fragments arranged by size:
3166 1193 775 642 523 317 110 49
27
SmlI C'TyrA_G
Cuts at: 1834 2614 2728 2941 3247 3955 4870 5364 5626
Size: 780 114 213 306 708 915 494 262
Cuts at: 5626 1834
Size: 3010
Fragments arranged by size:
3010 915 780 708 494 306 262 213
114
SnaBI TAC'GTA
Cuts at: 3792 3792
Size: 6802
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6802
SphI G_CATG'C
Cuts at: 3744 3853 4353 3744
Size: 109 500 6193
Fragments arranged by size:
6193 500 109
StuI AGG'CCT
Cuts at: 1476 1840 1476
Size: 364 6438
StyI C'CwwG_G
Cuts at: 734 1136 1238 1322 1580 1961 2042 3020 3934
Size: 402 102 84 258 381 81 978 914
Cuts at: 3934 4288 734
Size: 354 3248
Fragments arranged by size:
3248 978 914 402 381 354 258 102
84 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 825 2944 2984 3307 4383 4516 4975 5186 825
Size: 2119 40 323 1076 133 459 211 2441
Fragments arranged by size:
2441 2119 1076 459 323 211 133 40
TatI w'GTAC_w
Cuts at: 662 2900 3164 6317 662
Size: 2238 264 3153 1147
Fragments arranged by size:
3153 2238 1147 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 921 953 2494 2526 3088 3120 6319 6351 921
Size: 32 1541 32 562 32 3199 32 1372
Fragments arranged by size:
3199 1541 1372 562 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4597 4597
Size: 6802
XbaI T'CTAG_A
Cuts at: 3831 3831
Size: 6802
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 534 1101 2343 3342 4295 534
Size: 567 1242 999 953 3041
Fragments arranged by size:
3041 1242 999 953 567
XmaI C'CCGG_G
Cuts at: 184 233 875 902 2095 2870 2980 3297 3820
Size: 49 642 27 1193 775 110 317 523
Cuts at: 3820 184
Size: 3166
Fragments arranged by size:
3166 1193 775 642 523 317 110 49
27
ZraI GAC'GTC
Cuts at: 3498 3498
Size: 6802
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI AvrII BaeI BfrBI BmtI
BsiWI BsmI BstEII BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NsiI PacI PsiI
PspXI PsrI SrfI SspI SwaI XhoI XmnI