Restriction map for pF1KSDA1130
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AvrII       BalI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BsaAI       BsaI        BsgI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     ClaI        
CpoI        DraI        DrdI        DsaI        Eam1105I    
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HaeII       HincII      HindII      
HindIII     HpaI        KasI        KspI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       1241  GGTACC               G^GTACC
AccIII        501  TCCGGA               T^CCGGA
AcsI          960  GAATTT               [GA]^AATT[CT]
AcyI          864  GACGCC               G[GA]^CG[CT]C
AfaI         1242  GTAC                 GT^AC
AlwNI         755  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          960  GAATTT               [GA]^AATT[CT]
Asp700I      1173  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1241  GGTACC               G^GTACC
BfaI         1523  CTAG                 C^TAG
BlpI          294  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      294  GCTGAGC              GC^T[ACGT]AGC
BsaBI         939  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         864  GACGCC               G[GA]^CG[CT]C
BseAI         501  TCCGGA               T^CCGGA
BseRI         354  CTCCTC               GAGGAG(10/8)
BsiEI         108  CGGCCG               CG[GA][CT]^CG
BsmAI        1544  GTCTC                GTCTC(1/5)
BspEI         501  TCCGGA               T^CCGGA
BsrBI         169  CCGCTC               CCGCTC(-3/-3)
BsrDI         551  GCAATG               GCAATG(2/0)
BsrFI         305  ACCGGC               [GA]^CCGG[CT]
BssHII        116  GCGCGC               G^CGCGC
BstAPI        754  GCAGCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         294  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        305  ACCGGC               [GA]^CCGG[CT]
DraII         248  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        651  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          108  CGGCCG               C^GGCCG
EarI          429  GAAGAG               CTCTTC(1/4)
EclXI         108  CGGCCG               C^GGCCG
Eco52I        108  CGGCCG               C^GGCCG
EcoO109I      248  AGGACCC              [GA]G^G[ACGT]CC[CT]
Hin1I         864  GACGCC               G[GA]^CG[CT]C
KpnI         1241  GGTACC               GGTAC^C
Ksp632I       429  GAAGAG               CTCTTC(1/4)
MaeI         1523  CTAG                 C^TAG
MamI          939  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          501  TCCGGA               T^CCGGA
PpuMI         248  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         864  GACGCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI         1242  GTAC                 GT^AC
SmlI          820  CTTGAG               C^T[CT][GA]AG
XcmI         1338  CCAGAACACAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1173  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          512  GTCTAC               GT^[AC][GT]AC
             1255  GTCTAC              
AocI         1066  CCTGAGG              CC^T[ACGT]AGG
             1590  CCTCAGG             
AspHI         967  GAGCTC               G[AT]GC[AT]^C
             1264  GAGCTC              
AvaI          581  CCCGAG               C^[CT]CG[GA]G
             1576  CCCGAG              
AviII         419  TGCGCA               TGC^GCA
              548  TGCGCA              
BanI         1049  GGCACC               G^G[CT][GA]CC
             1241  GGTACC              
BbsI          505  GAAGAC               GAAGAC(2/6)
             1036  GTCTTC              
BglI         1022  GCCGGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1575  GCCCGAGGGGC         
BpuAI         505  GAAGAC               GAAGAC(2/6)
             1036  GTCTTC              
BsaWI         501  TCCGGA               [AT]^CCGG[AT]
              575  TCCGGT              
BsiHKAI       967  GAGCTC               G[AT]GC[AT]^C
             1264  GAGCTC              
BsoBI         581  CCCGAG               C^[CT]CG[GA]G
             1576  CCCGAG              
BspMI         362  ACCTGC               ACCTGC(4/8)
             1536  GCAGGT              
BsrI          785  ACTGG                ACTGG(1/-1)
             1498  CCAGT               
Bsu36I       1066  CCTGAGG              CC^T[ACGT]AGG
             1590  CCTCAGG             
Eco81I       1066  CCTGAGG              CC^T[ACGT]AGG
             1590  CCTCAGG             
EcoNI        1085  CCTACATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1522  CCTAGAGAAGG         
EcoT14I       533  CCAAGG               C^C[AT][AT]GG
             1605  CCTTGG              
FspI          419  TGCGCA               TGC^GCA
              548  TGCGCA              
HgaI          864  GACGC                GACGC(5/10)
              377  GCGTC               
MaeII         599  ACGT                 A^CGT
             1253  ACGT                
PleI          708  GAGTC                GAGTC(4/5)
             1542  GAGTC               
SacI          967  GAGCTC               GAGCT^C
             1264  GAGCTC              
StuI          212  AGGCCT               AGG^CCT
             1175  AGGCCT              
StyI          533  CCAAGG               C^C[AT][AT]GG
             1605  CCTTGG              
TfiI          884  GAATC                G^A[AT]TC
             1327  GAATC               
Tsp509I       739  AATT                 ^AATT
              961  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AvaII       
BamHI       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PstI        PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        Tru9I       TseI        
Tsp45I      TspRI       TthHB8I     XhoII       
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