Restriction map for
pF1KSDA1130
(Circular) (Six-base) MAPSORT of: pF1KSDA1130.seq Check: 2253 from: 1 to: 4765
pF1KSDA1130 4765 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1653 1653
Size: 4765
AccI GT'mk_AC
Cuts at: 639 1382 1801 639
Size: 743 419 3603
Fragments arranged by size:
3603 743 419
Acc65I G'GTAC_C
Cuts at: 1367 1779 1367
Size: 412 4353
AclI AA'CG_TT
Cuts at: 4605 4605
Size: 4765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 600 1086 1149 2588 3020 3769 4037 382
Size: 218 486 63 1439 432 749 268 1110
Fragments arranged by size:
1439 1110 749 486 432 268 218 63
AflIII A'CryG_T
Cuts at: 2115 3221 4198 2115
Size: 1106 977 2682
Fragments arranged by size:
2682 1106 977
AgeI A'CCGG_T
Cuts at: 3153 3153
Size: 4765
AleI CACnn'nnGTG
Cuts at: 826 826
Size: 4765
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1339 1371 1339
Size: 32 4733
AlwNI CAG_nnn'CTG
Cuts at: 886 2068 3637 886
Size: 1182 1569 2014
Fragments arranged by size:
2014 1569 1182
ApaLI G'TGCA_C
Cuts at: 3535 3535
Size: 4765
ApoI r'AATT_y
Cuts at: 1086 1767 4118 4374 1086
Size: 681 2351 256 1477
Fragments arranged by size:
2351 1477 681 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4765
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4765
AvaI C'yCGr_G
Cuts at: 707 1702 1783 707
Size: 995 81 3689
Fragments arranged by size:
3689 995 81
BamHI G'GATC_C
Cuts at: 173 653 1618 1788 173
Size: 480 965 170 3150
Fragments arranged by size:
3150 965 480 170
BanI G'GyrC_C
Cuts at: 1175 1367 1779 2193 2441 2476 1175
Size: 192 412 414 248 35 3464
Fragments arranged by size:
3464 414 412 248 192 35
BanII G_rGCy'C
Cuts at: 216 427 1097 1394 1746 1777 2807 216
Size: 211 670 297 352 31 1030 2174
Fragments arranged by size:
2174 1030 670 352 297 211 31
BbeI G_GCGC'C
Cuts at: 2197 2445 2197
Size: 248 4517
BbsI GAAGACnn'nnnn_
Cuts at: 638 1155 638
Size: 517 4248
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3424 4765 2661
Size: 763 1341 2661
Fragments arranged by size:
2661 1341 763
BclI T'GATC_A
Cuts at: 2283 2283
Size: 4765
BglI GCCn_nnn'nGGC
Cuts at: 1154 1707 1154
Size: 553 4212
BglII A'GATC_T
Cuts at: 2278 4317 2278
Size: 2039 2726
BlpI GC'TnA_GC
Cuts at: 421 1875 421
Size: 1454 3311
Bme1580I G_kGCm'C
Cuts at: 184 2388 2481 3539 184
Size: 2204 93 1058 1410
Fragments arranged by size:
2204 1410 1058 93
BmgBI CAC'GTC
Cuts at: 726 726
Size: 4765
BmrI ACTGGGnnnn_n'
Cuts at: 1618 1975 2056 2391 4537 1618
Size: 357 81 335 2146 1846
Fragments arranged by size:
2146 1846 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 4765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 427 933 1025 1150 1393 389
Size: 38 506 92 125 243 3761
Fragments arranged by size:
3761 506 243 125 92 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1939 2818 3312 3610 967
Size: 972 879 494 298 2122
Fragments arranged by size:
2122 972 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4469 4469
Size: 4765
BsaAI yAC'GTr
Cuts at: 1755 2116 1755
Size: 361 4404
BsaBI GATnn'nnATC
Cuts at: 6 1069 2277 4316 6
Size: 1063 1208 2039 455
Fragments arranged by size:
2039 1208 1063 455
BsaHI Gr'CG_yC
Cuts at: 991 2194 2442 3146 4642 991
Size: 1203 248 704 1496 1114
Fragments arranged by size:
1496 1203 1114 704 248
BsaWI w'CCGG_w
Cuts at: 627 701 1952 2473 3153 3427 3574 3985 627
Size: 74 1251 521 680 274 147 411 1407
Fragments arranged by size:
1407 1251 680 521 411 274 147 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 297 327 297
Size: 30 4735
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 471 471
Size: 4765
BseYI C'CCAG_C
Cuts at: 1694 2188 2946 3525 4021 1694
Size: 494 758 579 496 2438
Fragments arranged by size:
2438 758 579 496 494
BsiEI CG_ry'CG
Cuts at: 104 237 2305 2351 3561 104
Size: 133 2068 46 1210 1308
Fragments arranged by size:
2068 1308 1210 133 46
BsiHKAI G_wGCw'C
Cuts at: 1097 1394 1777 2555 2745 3539 1097
Size: 297 383 778 190 794 2323
Fragments arranged by size:
2323 794 778 383 297 190
Bsp1286I G_dGCh'C
Cuts at: 184 216 427 1097 1394 1746 1777 2388 2481
Size: 32 211 670 297 352 31 611 93
Cuts at: 2481 2555 2745 2807 3539 184
Size: 74 190 62 732 1410
Fragments arranged by size:
1410 732 670 611 352 297 211 190
93 74 62 32 31
BspEI T'CCGG_A
Cuts at: 627 3985 627
Size: 3358 1407
BspHI T'CATG_A
Cuts at: 3941 3941
Size: 4765
BspMI ACCTGCnnnn'nnnn_
Cuts at: 497 1653 1813 2027 2329 2710 497
Size: 1156 160 214 302 381 2552
Fragments arranged by size:
2552 1156 381 302 214 160
BsrBI CCG'CTC
Cuts at: 50 297 3055 3109 4595 4760 50
Size: 247 2758 54 1486 165 55
Fragments arranged by size:
2758 1486 247 165 55 54
BsrDI GCAATG_nn'
Cuts at: 684 2675 684
Size: 1991 2774
BsrFI r'CCGG_y
Cuts at: 431 2761 2942 3153 431
Size: 2330 181 211 2043
Fragments arranged by size:
2330 2043 211 181
BssHII G'CGCG_C
Cuts at: 242 242
Size: 4765
BssSI C'ACGA_G
Cuts at: 3034 3394 3034
Size: 360 4405
BstAPI GCAn_nnn'nTGC
Cuts at: 886 2140 886
Size: 1254 3511
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4765
BstYI r'GATC_y
Cuts at: 1 134 173 624 653 938 1499 1618 1788
Size: 133 39 451 29 285 561 119 170
Cuts at: 1788 2255 2278 2613 2859 3873 3959 3971 4317
Size: 467 23 335 246 1014 86 12 346
Cuts at: 4317 1
Size: 449
Fragments arranged by size:
1014 561 467 451 449 346 335 285
246 170 133 119 86 39 29 23
12
Bsu36I CC'TnA_GG
Cuts at: 1193 1717 1193
Size: 524 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 135 2713 2894 3057 135
Size: 2578 181 163 1843
Fragments arranged by size:
2578 1843 181 163
BtsI GCAGTG_nn'
Cuts at: 762 1232 1317 2006 762
Size: 470 85 689 3521
Fragments arranged by size:
3521 689 470 85
DraI TTT'AAA
Cuts at: 1762 1762
Size: 4765
DraIII CAC_nnn'GTG
Cuts at: 782 782
Size: 4765
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3329 2469
Size: 860 3905
EaeI y'GGCC_r
Cuts at: 234 350 1289 2348 2522 2913 2940 4287 234
Size: 116 939 1059 174 391 27 1347 712
Fragments arranged by size:
1347 1059 939 712 391 174 116 27
EagI C'GGCC_G
Cuts at: 234 2348 234
Size: 2114 2651
EarI CTCTTCn'nnn_
Cuts at: 550 2786 2996 550
Size: 2236 210 2319
Fragments arranged by size:
2319 2236 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 736 2971 3283 3429 4575 736
Size: 2235 312 146 1146 926
Fragments arranged by size:
2235 1146 926 312 146
EcoICRI GAG'CTC
Cuts at: 1095 1392 1775 1095
Size: 297 383 4085
Fragments arranged by size:
4085 383 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 389 427 600 933 1025 1086 1149 1150
Size: 7 38 173 333 92 61 63 1
Cuts at: 1150 1393 2588 3020 3769 4037 382
Size: 243 1195 432 749 268 1110
Fragments arranged by size:
1195 1110 749 432 333 268 243 173
92 63 61 38 7 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1215 1652 1215
Size: 437 4328
EcoO109I rG'GnC_Cy
Cuts at: 375 1902 375
Size: 1527 3238
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4765
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4765
FspI TGC'GCA
Cuts at: 547 676 2544 547
Size: 129 1868 2768
Fragments arranged by size:
2768 1868 129
HaeII r_GCGC'y
Cuts at: 2197 2445 3469 4448 2197
Size: 248 1024 979 2514
Fragments arranged by size:
2514 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 297 329 604 636 1610 1642 2605 2635 2637
Size: 32 275 32 974 32 963 30 2
Cuts at: 2637 2667 297
Size: 30 2395
Fragments arranged by size:
2395 974 963 275 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1802 1802
Size: 4765
KasI G'GCGC_C
Cuts at: 2193 2441 2193
Size: 248 4517
KpnI G_GTAC'C
Cuts at: 1371 1783 1371
Size: 412 4353
MluI A'CGCG_T
Cuts at: 4198 4198
Size: 4765
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 245 3436 3620 245
Size: 3191 184 1390
Fragments arranged by size:
3191 1390 184
MscI TGG'CCA
Cuts at: 2524 2524
Size: 4765
MslI CAynn'nnrTG
Cuts at: 604 826 1603 2752 2879 604
Size: 222 777 1149 127 2490
Fragments arranged by size:
2490 1149 777 222 127
MspA1I CmG'CkG
Cuts at: 360 405 461 804 1364 1474 1874 2188 2548
Size: 45 56 343 560 110 400 314 360
Cuts at: 2548 3563 3808 360
Size: 1015 245 1317
Fragments arranged by size:
1317 1015 560 400 360 343 314 245
110 56 45
NaeI GCC'GGC
Cuts at: 2944 2944
Size: 4765
NarI GG'CG_CC
Cuts at: 2194 2442 2194
Size: 248 4517
NgoMIV G'CCGG_C
Cuts at: 2942 2942
Size: 4765
NruI TCG'CGA
Cuts at: 3126 3126
Size: 4765
NspI r_CATG'y
Cuts at: 1816 2316 3225 1816
Size: 500 909 3356
Fragments arranged by size:
3356 909 500
PciI A'CATG_T
Cuts at: 3221 3221
Size: 4765
PfoI T'CCnGG_A
Cuts at: 440 590 440
Size: 150 4615
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4765
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3904 3936 3904
Size: 32 4733
PpuMI rG'GwC_Cy
Cuts at: 375 375
Size: 4765
PshAI GACnn'nnGTC
Cuts at: 994 994
Size: 4765
PstI C_TGCA'G
Cuts at: 735 803 1147 1239 1507 1637 1810 2495 735
Size: 68 344 92 268 130 173 685 3005
Fragments arranged by size:
3005 685 344 268 173 130 92 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4765
PvuII CAG'CTG
Cuts at: 360 405 461 1474 2188 2548 360
Size: 45 56 1013 714 360 2577
Fragments arranged by size:
2577 1013 714 360 56 45
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4765
SacI G_AGCT'C
Cuts at: 1097 1394 1777 1097
Size: 297 383 4085
Fragments arranged by size:
4085 383 297
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4765
SapI GCTCTTCn'nnn_
Cuts at: 2786 2996 2786
Size: 210 4555
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4765
ScaI AGT'ACT
Cuts at: 4282 4282
Size: 4765
SfcI C'TryA_G
Cuts at: 33 381 731 799 1143 1235 1503 1633 1806
Size: 348 350 68 344 92 268 130 173
Cuts at: 1806 2491 3486 3677 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 685 350 348 344 268 191
173 130 92 68
SfoI GGC'GCC
Cuts at: 2195 2443 2195
Size: 248 4517
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4765
SmaI CCC'GGG
Cuts at: 1785 1785
Size: 4765
SmlI C'TyrA_G
Cuts at: 946 1918 2833 3327 3589 946
Size: 972 915 494 262 2122
Fragments arranged by size:
2122 972 915 494 262
SnaBI TAC'GTA
Cuts at: 1755 1755
Size: 4765
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4765
SphI G_CATG'C
Cuts at: 1816 2316 1816
Size: 500 4265
StuI AGG'CCT
Cuts at: 340 1303 340
Size: 963 3802
StyI C'CwwG_G
Cuts at: 659 1731 1897 2251 659
Size: 1072 166 354 3173
Fragments arranged by size:
3173 1072 354 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2346 2479 2938 3149 2346
Size: 133 459 211 3962
Fragments arranged by size:
3962 459 211 133
TatI w'GTAC_w
Cuts at: 4280 4280
Size: 4765
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 208 240 1001 1033 1104 1136 4282 4314 208
Size: 32 761 32 71 32 3146 32 659
Fragments arranged by size:
3146 761 659 71 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2560 2560
Size: 4765
XbaI T'CTAG_A
Cuts at: 1794 1794
Size: 4765
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1471 2258 1471
Size: 787 3978
XmaI C'CCGG_G
Cuts at: 1783 1783
Size: 4765
XmnI GAAnn'nnTTC
Cuts at: 1303 1303
Size: 4765
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PciI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I BtgI ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NsiI PacI PasI PflMI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI SwaI XhoI ZraI