Restriction map for pF1KSDA1130
 (Linear) (Six-base) MAPSORT of: KIAA1130.seq  Check: 3492  from: 1  to: 1626



KIAA1130.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1527    1626 
   Size:       1527      99
 
AccI GT'mk_AC

Cuts at:      0     513    1256    1626 
   Size:        513     743     370

  Fragments arranged by size: 

                 743     513     370
 
Acc65I G'GTAC_C

Cuts at:      0    1241    1626 
   Size:       1241     385
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     474     960    1023    1626 
   Size:        256     218     486      63     603

  Fragments arranged by size: 

                 603     486     256     218      63
 
AleI CACnn'nnGTG

Cuts at:      0     700    1626 
   Size:        700     926
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1213    1245    1626 
   Size:       1213      32     381

  Fragments arranged by size: 

                1213     381      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     760    1626 
   Size:        760     866
 
ApoI r'AATT_y

Cuts at:      0     960    1626 
   Size:        960     666
 
AvaI C'yCGr_G

Cuts at:      0     581    1576    1626 
   Size:        581     995      50

  Fragments arranged by size: 

                 995     581      50
 
BamHI G'GATC_C

Cuts at:      0      47     527    1492    1626 
   Size:         47     480     965     134

  Fragments arranged by size: 

                 965     480     134      47
 
BanI G'GyrC_C

Cuts at:      0    1049    1241    1626 
   Size:       1049     192     385

  Fragments arranged by size: 

                1049     385     192
 
BanII G_rGCy'C

Cuts at:      0      90     301     971    1268    1620    1626 
   Size:         90     211     670     297     352       6

  Fragments arranged by size: 

                 670     352     297     211      90       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     512    1029    1626 
   Size:        512     517     597

  Fragments arranged by size: 

                 597     517     512
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1028    1581    1626 
   Size:       1028     553      45

  Fragments arranged by size: 

                1028     553      45
 
BlpI GC'TnA_GC

Cuts at:      0     295    1626 
   Size:        295    1331
 
Bme1580I G_kGCm'C

Cuts at:      0      58    1626 
   Size:         58    1568
 
BmgBI CAC'GTC

Cuts at:      0     600    1626 
   Size:        600    1026
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1492    1626 
   Size:       1492     134
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     301     807     899    1024    1267    1626 
   Size:        263      38     506      92     125     243     359

  Fragments arranged by size: 

                 506     359     263     243     125      92      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841    1626 
   Size:        841     785
 
BsaBI GATnn'nnATC

Cuts at:      0     943    1626 
   Size:        943     683
 
BsaHI Gr'CG_yC

Cuts at:      0     865    1626 
   Size:        865     761
 
BsaWI w'CCGG_w

Cuts at:      0     501     575    1626 
   Size:        501      74    1051

  Fragments arranged by size: 

                1051     501      74
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     171     201    1626 
   Size:        171      30    1425

  Fragments arranged by size: 

                1425     171      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     345    1626 
   Size:        345    1281
 
BseYI C'CCAG_C

Cuts at:      0    1568    1626 
   Size:       1568      58
 
BsiEI CG_ry'CG

Cuts at:      0     111    1626 
   Size:        111    1515
 
BsiHKAI G_wGCw'C

Cuts at:      0     971    1268    1626 
   Size:        971     297     358

  Fragments arranged by size: 

                 971     358     297
 
Bsp1286I G_dGCh'C

Cuts at:      0      58      90     301     971    1268    1620    1626 
   Size:         58      32     211     670     297     352       6

  Fragments arranged by size: 

                 670     352     297     211      58      32       6
 
BspEI T'CCGG_A

Cuts at:      0     501    1626 
   Size:        501    1125
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     371    1527    1626 
   Size:        371    1156      99

  Fragments arranged by size: 

                1156     371      99
 
BsrBI CCG'CTC

Cuts at:      0     171    1626 
   Size:        171    1455
 
BsrDI GCAATG_nn'

Cuts at:      0     558    1626 
   Size:        558    1068
 
BsrFI r'CCGG_y

Cuts at:      0     305    1626 
   Size:        305    1321
 
BssHII G'CGCG_C

Cuts at:      0     116    1626 
   Size:        116    1510
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     760    1626 
   Size:        760     866
 
BstYI r'GATC_y

Cuts at:      0       8      47     498     527     812    1373    1492    1626 
   Size:          8      39     451      29     285     561     119     134

  Fragments arranged by size: 

                 561     451     285     134     119      39      29       8
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1067    1591    1626 
   Size:       1067     524      35

  Fragments arranged by size: 

                1067     524      35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       9    1626 
   Size:          9    1617
 
BtsI GCAGTG_nn'

Cuts at:      0     636    1106    1191    1626 
   Size:        636     470      85     435

  Fragments arranged by size: 

                 636     470     435      85
 
DraIII CAC_nnn'GTG

Cuts at:      0     656    1626 
   Size:        656     970
 
EaeI y'GGCC_r

Cuts at:      0     108     224    1163    1626 
   Size:        108     116     939     463

  Fragments arranged by size: 

                 939     463     116     108
 
EagI C'GGCC_G

Cuts at:      0     108    1626 
   Size:        108    1518
 
EarI CTCTTCn'nnn_

Cuts at:      0     424    1626 
   Size:        424    1202
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     610    1626 
   Size:        610    1016
 
EcoICRI GAG'CTC

Cuts at:      0     969    1266    1626 
   Size:        969     297     360

  Fragments arranged by size: 

                 969     360     297
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     263     301     474     807     899     960    1023 
   Size:        256       7      38     173     333      92      61      63

Cuts at:   1023    1024    1267    1626 
   Size:          1     243     359

  Fragments arranged by size: 

                 359     333     256     243     173      92      63      61
                  38       7       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1089    1526    1626 
   Size:       1089     437     100

  Fragments arranged by size: 

                1089     437     100
 
EcoO109I rG'GnC_Cy

Cuts at:      0     249    1626 
   Size:        249    1377
 
FspI TGC'GCA

Cuts at:      0     421     550    1626 
   Size:        421     129    1076

  Fragments arranged by size: 

                1076     421     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     171     203     478     510    1484    1516    1626 
   Size:        171      32     275      32     974      32     110

  Fragments arranged by size: 

                 974     275     171     110      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0    1245    1626 
   Size:       1245     381
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     119    1626 
   Size:        119    1507
 
MslI CAynn'nnrTG

Cuts at:      0     478     700    1477    1626 
   Size:        478     222     777     149

  Fragments arranged by size: 

                 777     478     222     149
 
MspA1I CmG'CkG

Cuts at:      0     234     279     335     678    1238    1348    1626 
   Size:        234      45      56     343     560     110     278

  Fragments arranged by size: 

                 560     343     278     234     110      56      45
 
PfoI T'CCnGG_A

Cuts at:      0     314     464    1626 
   Size:        314     150    1162

  Fragments arranged by size: 

                1162     314     150
 
PpuMI rG'GwC_Cy

Cuts at:      0     249    1626 
   Size:        249    1377
 
PshAI GACnn'nnGTC

Cuts at:      0     868    1626 
   Size:        868     758
 
PstI C_TGCA'G

Cuts at:      0     609     677    1021    1113    1381    1511    1626 
   Size:        609      68     344      92     268     130     115

  Fragments arranged by size: 

                 609     344     268     130     115      92      68
 
PvuII CAG'CTG

Cuts at:      0     234     279     335    1348    1626 
   Size:        234      45      56    1013     278

  Fragments arranged by size: 

                1013     278     234      56      45
 
SacI G_AGCT'C

Cuts at:      0     971    1268    1626 
   Size:        971     297     358

  Fragments arranged by size: 

                 971     358     297
 
SfcI C'TryA_G

Cuts at:      0     255     605     673    1017    1109    1377    1507    1626 
   Size:        255     350      68     344      92     268     130     119

  Fragments arranged by size: 

                 350     344     268     255     130     119      92      68
            
 
SmlI C'TyrA_G

Cuts at:      0     820    1626 
   Size:        820     806
 
StuI AGG'CCT

Cuts at:      0     214    1177    1626 
   Size:        214     963     449

  Fragments arranged by size: 

                 963     449     214
 
StyI C'CwwG_G

Cuts at:      0     533    1605    1626 
   Size:        533    1072      21

  Fragments arranged by size: 

                1072     533      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      82     114     875     907     978    1010    1626 
   Size:         82      32     761      32      71      32     616

  Fragments arranged by size: 

                 761     616      82      71      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1345    1626 
   Size:       1345     281
 
XmnI GAAnn'nnTTC

Cuts at:      0    1177    1626 
   Size:       1177     449
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AleI     AloI    AlwNI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BpmI    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII   BstAPI    BstYI   Bsu36I    BtgZI     BtsI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FspI    Hin4I     KpnI     MmeI     MslI   MspA1I     PfoI
    PpuMI    PshAI     PstI    PvuII     SacI     SfcI     SmlI     StuI
     StyI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AlfI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BmtI     BplI
   Bpu10I     BsaI    BsaAI     BsgI    BsiWI     BsmI    BsmBI    BspHI
    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI     ClaI
    CspCI     DraI     DrdI    EcoRI    EcoRV     FalI     FseI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI
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