Restriction map for
pF1KSDA1125
(Circular) (Six-base) MAPSORT of: pF1KSDA1125.seq Check: 8253 from: 1 to: 6695
pF1KSDA1125 6695 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 3454 3454
Size: 6695
AccI GT'mk_AC
Cuts at: 3733 3733
Size: 6695
Acc65I G'GTAC_C
Cuts at: 3711 3711
Size: 6695
AclI AA'CG_TT
Cuts at: 6535 6535
Size: 6695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1485 2215 3401 4520 4952 5699 5967 1000
Size: 485 730 1186 1119 432 747 268 1728
Fragments arranged by size:
1728 1186 1119 747 730 485 432 268
AflIII A'CryG_T
Cuts at: 3607 4047 5151 6128 3607
Size: 440 1104 977 4174
Fragments arranged by size:
4174 1104 977 440
AgeI A'CCGG_T
Cuts at: 2406 5083 2406
Size: 2677 4018
AleI CACnn'nnGTG
Cuts at: 1502 2377 2782 2839 1502
Size: 875 405 57 5358
Fragments arranged by size:
5358 875 405 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1052 1384 1052
Size: 332 6363
AlwNI CAG_nnn'CTG
Cuts at: 355 1067 2104 3042 3111 3260 3325 4000 5567
Size: 712 1037 938 69 149 65 675 1567
Cuts at: 5567 355
Size: 1483
Fragments arranged by size:
1567 1483 1037 938 712 675 149 69
65
ApaLI G'TGCA_C
Cuts at: 1444 5465 1444
Size: 4021 2674
ApoI r'AATT_y
Cuts at: 1106 3699 6048 6304 1106
Size: 2593 2349 256 1497
Fragments arranged by size:
2593 2349 1497 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6695
AvaI C'yCGr_G
Cuts at: 446 2820 3396 3715 446
Size: 2374 576 319 3426
Fragments arranged by size:
3426 2374 576 319
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3612 3645 3612
Size: 33 6662
BamHI G'GATC_C
Cuts at: 3720 3720
Size: 6695
BanI G'GyrC_C
Cuts at: 2446 3711 4125 4373 4408 2446
Size: 1265 414 248 35 4733
Fragments arranged by size:
4733 1265 414 248 35
BanII G_rGCy'C
Cuts at: 440 1456 2761 3059 3394 3709 4739 440
Size: 1016 1305 298 335 315 1030 2396
Fragments arranged by size:
2396 1305 1030 1016 335 315 298
BbeI G_GCGC'C
Cuts at: 2450 4129 4377 2450
Size: 1679 248 4768
Fragments arranged by size:
4768 1679 248
BbsI GAAGACnn'nnnn_
Cuts at: 1976 2288 2681 1976
Size: 312 393 5990
Fragments arranged by size:
5990 393 312
BbvCI CC'TCA_GC
Cuts at: 2880 3013 3332 3350 2880
Size: 133 319 18 6225
Fragments arranged by size:
6225 319 133 18
BciVI GTATCCnnnnn_n'
Cuts at: 4593 5354 6695 4593
Size: 761 1341 4593
Fragments arranged by size:
4593 1341 761
BclI T'GATC_A
Cuts at: 4215 4215
Size: 6695
BfrBI ATG'CAT
Cuts at: 539 640 539
Size: 101 6594
BglI GCCn_nnn'nGGC
Cuts at: 766 766
Size: 6695
BglII A'GATC_T
Cuts at: 4210 6247 4210
Size: 2037 4658
BlpI GC'TnA_GC
Cuts at: 1720 3807 1720
Size: 2087 4608
Bme1580I G_kGCm'C
Cuts at: 1384 1448 4320 4413 5469 1384
Size: 64 2872 93 1056 2610
Fragments arranged by size:
2872 2610 1056 93 64
BmgBI CAC'GTC
Cuts at: 1461 1490 2078 2468 2768 1461
Size: 29 588 390 300 5388
Fragments arranged by size:
5388 588 390 300 29
BmrI ACTGGGnnnn_n'
Cuts at: 2041 2512 3309 3907 3988 4323 6467 2041
Size: 471 797 598 81 335 2144 2269
Fragments arranged by size:
2269 2144 797 598 471 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3495 3531 5062 3495
Size: 36 1531 5128
Fragments arranged by size:
5128 1531 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 3019 3133 3178 3486 2126
Size: 893 114 45 308 5335
Fragments arranged by size:
5335 893 308 114 45
Bpu10I CC'TnA_GC
Cuts at: 2132 2880 3013 3293 3332 3350 2132
Size: 748 133 280 39 18 5477
Fragments arranged by size:
5477 748 280 133 39 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 3871 4750 5242 5540 583
Size: 3288 879 492 298 1738
Fragments arranged by size:
3288 1738 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 538 3181 6399 538
Size: 2643 3218 834
Fragments arranged by size:
3218 2643 834
BsaAI yAC'GTr
Cuts at: 1173 3687 4048 1173
Size: 2514 361 3820
Fragments arranged by size:
3820 2514 361
BsaBI GATnn'nnATC
Cuts at: 6 4209 6246 6
Size: 4203 2037 455
Fragments arranged by size:
4203 2037 455
BsaHI Gr'CG_yC
Cuts at: 2447 3451 3511 4126 4374 5076 6572 2447
Size: 1004 60 615 248 702 1496 2570
Fragments arranged by size:
2570 1496 1004 702 615 248 60
BsaWI w'CCGG_w
Cuts at: 2406 3884 4405 5083 5357 5504 5915 2406
Size: 1478 521 678 274 147 411 3186
Fragments arranged by size:
3186 1478 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1438 1468 1636 1666 3570 3600 1438
Size: 30 168 30 1904 30 4533
Fragments arranged by size:
4533 1904 168 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1528 1601 2077 3320 3384 3507 3637 1528
Size: 73 476 1243 64 123 130 4586
Fragments arranged by size:
4586 1243 476 130 123 73 64
BseYI C'CCAG_C
Cuts at: 193 398 1276 4120 4878 5455 5951 193
Size: 205 878 2844 758 577 496 937
Fragments arranged by size:
2844 937 878 758 577 496 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 2587 3028 3294 735
Size: 1852 441 266 4136
Fragments arranged by size:
4136 1852 441 266
BsiEI CG_ry'CG
Cuts at: 104 1874 2564 4237 4283 5491 104
Size: 1770 690 1673 46 1208 1308
Fragments arranged by size:
1770 1673 1308 1208 690 46
BsiHKAI G_wGCw'C
Cuts at: 440 1432 1448 1543 1883 2414 2836 3059 3299
Size: 992 16 95 340 531 422 223 240
Cuts at: 3299 3394 3403 3709 4487 4677 5469 440
Size: 95 9 306 778 190 792 1666
Fragments arranged by size:
1666 992 792 778 531 422 340 306
240 223 190 95 95 16 9
BsmI GAATG_Cn'
Cuts at: 541 640 732 541
Size: 99 92 6504
Fragments arranged by size:
6504 99 92
BsmBI CGTCTCn'nnnn_
Cuts at: 2203 3442 3502 2203
Size: 1239 60 5396
Fragments arranged by size:
5396 1239 60
Bsp1286I G_dGCh'C
Cuts at: 440 1384 1432 1448 1456 1543 1883 2414 2761
Size: 944 48 16 8 87 340 531 347
Cuts at: 2761 2836 3059 3299 3394 3403 3709 4320 4413
Size: 75 223 240 95 9 306 611 93
Cuts at: 4413 4487 4677 4739 5469 440
Size: 74 190 62 730 1666
Fragments arranged by size:
1666 944 730 611 531 347 340 306
240 223 190 95 93 87 75 74
62 48 16 9 8
BspEI T'CCGG_A
Cuts at: 5915 5915
Size: 6695
BspHI T'CATG_A
Cuts at: 5871 5871
Size: 6695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 602 1020 1778 2870 3745 3959 4261 4642 602
Size: 418 758 1092 875 214 302 381 2655
Fragments arranged by size:
2655 1092 875 758 418 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2420 3123 4987 5041 6525 6690 50
Size: 2370 703 1864 54 1484 165 55
Fragments arranged by size:
2370 1864 1484 703 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4607 4607
Size: 6695
BsrFI r'CCGG_y
Cuts at: 1282 1660 2406 3128 4693 4874 5083 1282
Size: 378 746 722 1565 181 209 2894
Fragments arranged by size:
2894 1565 746 722 378 209 181
BsrGI T'GTAC_A
Cuts at: 712 712
Size: 6695
BssSI C'ACGA_G
Cuts at: 4966 5324 4966
Size: 358 6337
BstAPI GCAn_nnn'nTGC
Cuts at: 2634 4072 2634
Size: 1438 5257
BstBI TT'CG_AA
Cuts at: 110 5056 110
Size: 4946 1749
BstEII G'GTnAC_C
Cuts at: 2478 2784 2811 2478
Size: 306 27 6362
Fragments arranged by size:
6362 306 27
BstXI CCAn_nnnn'nTGG
Cuts at: 2379 2781 2379
Size: 402 6293
BstYI r'GATC_y
Cuts at: 1 150 1349 3720 4187 4210 4545 4791 5803
Size: 149 1199 2371 467 23 335 246 1012
Cuts at: 5803 5889 5901 6247 1
Size: 86 12 346 449
Fragments arranged by size:
2371 1199 1012 467 449 346 335 246
149 86 23 12
BtgI C'CryG_G
Cuts at: 125 1520 2438 2474 3089 125
Size: 1395 918 36 615 3731
Fragments arranged by size:
3731 1395 918 615 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2786 2853 3120 4645 4826 4989 2786
Size: 67 267 1525 181 163 4492
Fragments arranged by size:
4492 1525 267 181 163 67
BtsI GCAGTG_nn'
Cuts at: 1286 2873 3208 3591 3938 1286
Size: 1587 335 383 347 4043
Fragments arranged by size:
4043 1587 383 347 335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 944 979 2030 2065 3456 3491 944
Size: 35 1051 35 1391 35 4148
Fragments arranged by size:
4148 1391 1051 35 35 35
DraI TTT'AAA
Cuts at: 3694 3694
Size: 6695
DrdI GACnn_nn'nnGTC
Cuts at: 1030 4401 5259 1030
Size: 3371 858 2466
Fragments arranged by size:
3371 2466 858
EaeI y'GGCC_r
Cuts at: 216 1871 2561 3425 4280 4454 4845 4872 6217
Size: 1655 690 864 855 174 391 27 1345
Cuts at: 6217 216
Size: 694
Fragments arranged by size:
1655 1345 864 855 694 690 391 174
27
EagI C'GGCC_G
Cuts at: 1871 2561 4280 1871
Size: 690 1719 4286
Fragments arranged by size:
4286 1719 690
EarI CTCTTCn'nnn_
Cuts at: 1443 2427 3589 3634 4718 4928 1443
Size: 984 1162 45 1084 210 3210
Fragments arranged by size:
3210 1162 1084 984 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2415 3109 4903 5213 5359 6505 2415
Size: 694 1794 310 146 1146 2605
Fragments arranged by size:
2605 1794 1146 694 310 146
EcoICRI GAG'CTC
Cuts at: 438 3057 3392 3707 438
Size: 2619 335 315 3426
Fragments arranged by size:
3426 2619 335 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1485 2126 2215 3019 3133 3178 3401 3486
Size: 485 641 89 804 114 45 223 85
Cuts at: 3486 4520 4952 5699 5967 1000
Size: 1034 432 747 268 1728
Fragments arranged by size:
1728 1034 804 747 641 485 432 268
223 114 89 85 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3115 3115
Size: 6695
EcoO109I rG'GnC_Cy
Cuts at: 1064 3834 1064
Size: 2770 3925
EcoRI G'AATT_C
Cuts at: 3699 3699
Size: 6695
EcoRV GAT'ATC
Cuts at: 132 2707 132
Size: 2575 4120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1155 1341 4714 1155
Size: 186 3373 3136
Fragments arranged by size:
3373 3136 186
FspI TGC'GCA
Cuts at: 4476 4476
Size: 6695
HaeII r_GCGC'y
Cuts at: 1283 1776 2450 3434 4129 4377 5399 6378 1283
Size: 493 674 984 695 248 1022 979 1600
Fragments arranged by size:
1600 1022 984 979 695 674 493 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1636 1668 1936 1968 2625 2657 2691
Size: 32 1462 32 268 32 657 32 34
Cuts at: 2691 2723 4537 4567 4569 4599 142
Size: 32 1814 30 2 30 2238
Fragments arranged by size:
2238 1814 1462 657 268 34 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2853 3734 2853
Size: 881 5814
KasI G'GCGC_C
Cuts at: 2446 4125 4373 2446
Size: 1679 248 4768
Fragments arranged by size:
4768 1679 248
KpnI G_GTAC'C
Cuts at: 3715 3715
Size: 6695
MluI A'CGCG_T
Cuts at: 6128 6128
Size: 6695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1385 1481 3143 3226 3556 3560 5366 5550 1385
Size: 96 1662 83 330 4 1806 184 2530
Fragments arranged by size:
2530 1806 1662 330 184 96 83 4
MscI TGG'CCA
Cuts at: 218 4456 218
Size: 4238 2457
MslI CAynn'nnrTG
Cuts at: 565 1502 2377 2782 2839 4684 4811 565
Size: 937 875 405 57 1845 127 2449
Fragments arranged by size:
2449 1845 937 875 405 127 57
MspA1I CmG'CkG
Cuts at: 1797 2440 2571 2884 2896 3156 3457 3806 4120
Size: 643 131 313 12 260 301 349 314
Cuts at: 4120 4480 5493 5738 1797
Size: 360 1013 245 2754
Fragments arranged by size:
2754 1013 643 360 349 314 313 301
260 245 131 12
NaeI GCC'GGC
Cuts at: 1284 4876 1284
Size: 3592 3103
NarI GG'CG_CC
Cuts at: 2447 4126 4374 2447
Size: 1679 248 4768
Fragments arranged by size:
4768 1679 248
NcoI C'CATG_G
Cuts at: 125 1520 3089 125
Size: 1395 1569 3731
Fragments arranged by size:
3731 1569 1395
NgoMIV G'CCGG_C
Cuts at: 1282 4874 1282
Size: 3592 3103
NsiI A_TGCA'T
Cuts at: 541 642 541
Size: 101 6594
NspI r_CATG'y
Cuts at: 2628 3748 4248 5155 2628
Size: 1120 500 907 4168
Fragments arranged by size:
4168 1120 907 500
PasI CC'CwG_GG
Cuts at: 2694 3384 2694
Size: 690 6005
PciI A'CATG_T
Cuts at: 5151 5151
Size: 6695
PflMI CCAn_nnn'nTGG
Cuts at: 702 702
Size: 6695
PfoI T'CCnGG_A
Cuts at: 1529 3556 1529
Size: 2027 4668
PmeI GTTT'AAAC
Cuts at: 3694 3694
Size: 6695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5834 5866 5834
Size: 32 6663
PsiI TTA'TAA
Cuts at: 806 806
Size: 6695
PspXI vC'TCGA_Gb
Cuts at: 3396 3396
Size: 6695
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3241 3273 3241
Size: 32 6663
PstI C_TGCA'G
Cuts at: 165 3742 4427 165
Size: 3577 685 2433
Fragments arranged by size:
3577 2433 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6695
PvuII CAG'CTG
Cuts at: 1797 2884 3156 3457 4120 4480 1797
Size: 1087 272 301 663 360 4012
Fragments arranged by size:
4012 1087 663 360 301 272
RsrII CG'GwC_CG
Cuts at: 4890 4890
Size: 6695
SacI G_AGCT'C
Cuts at: 440 3059 3394 3709 440
Size: 2619 335 315 3426
Fragments arranged by size:
3426 2619 335 315
SacII CC_GC'GG
Cuts at: 2441 2441
Size: 6695
SalI G'TCGA_C
Cuts at: 3732 3732
Size: 6695
SapI GCTCTTCn'nnn_
Cuts at: 2427 3634 4718 4928 2427
Size: 1207 1084 210 4194
Fragments arranged by size:
4194 1207 1084 210
SbfI CC_TGCA'GG
Cuts at: 3742 3742
Size: 6695
ScaI AGT'ACT
Cuts at: 6212 6212
Size: 6695
SfcI C'TryA_G
Cuts at: 33 161 3738 4423 5416 5607 33
Size: 128 3577 685 993 191 1121
Fragments arranged by size:
3577 1121 993 685 191 128
SfoI GGC'GCC
Cuts at: 2448 4127 4375 2448
Size: 1679 248 4768
Fragments arranged by size:
4768 1679 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6695
SgrAI Cr'CCGG_yG
Cuts at: 2406 2406
Size: 6695
SmaI CCC'GGG
Cuts at: 448 3717 448
Size: 3269 3426
SmlI C'TyrA_G
Cuts at: 598 3396 3850 4765 5257 5519 598
Size: 2798 454 915 492 262 1774
Fragments arranged by size:
2798 1774 915 492 454 262
SnaBI TAC'GTA
Cuts at: 3687 3687
Size: 6695
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6695
SphI G_CATG'C
Cuts at: 3748 4248 3748
Size: 500 6195
StyI C'CwwG_G
Cuts at: 125 1520 3089 3540 3829 4183 125
Size: 1395 1569 451 289 354 2637
Fragments arranged by size:
2637 1569 1395 451 354 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1251 1719 3328 4278 4411 4870 5079 1251
Size: 468 1609 950 133 459 209 2867
Fragments arranged by size:
2867 1609 950 468 459 209 133
TatI w'GTAC_w
Cuts at: 712 2909 6210 712
Size: 2197 3301 1197
Fragments arranged by size:
3301 2197 1197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1438 1470 1737 1769 2504 2536 3289 3321 3570
Size: 32 267 32 735 32 753 32 249
Cuts at: 3570 3602 6212 6244 1438
Size: 32 2610 32 1889
Fragments arranged by size:
2610 1889 753 735 267 249 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 314 4492 314
Size: 4178 2517
XbaI T'CTAG_A
Cuts at: 3726 3726
Size: 6695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 494 1158 1527 2778 4190 494
Size: 664 369 1251 1412 2999
Fragments arranged by size:
2999 1412 1251 664 369
XhoI C'TCGA_G
Cuts at: 3396 3396
Size: 6695
XmaI C'CCGG_G
Cuts at: 446 3715 446
Size: 3269 3426
XmnI GAAnn'nnTTC
Cuts at: 388 388
Size: 6695
ZraI GAC'GTC
Cuts at: 3452 3452
Size: 6695
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BcgI BmtI BsiWI BssHII BstZ17I Bsu36I ClaI DraIII
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PmlI PpuMI PshAI PspOMI SanDI SexAI
SfiI SrfI SspI StuI SwaI