Restriction map for pF1KSDA1087
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AgeI        AlwNI       AocI        ApoI        AscI        
AseI        AsnI        Asp718I     AvrII       BamHI       
BbvCI       BciVI       BclI        BglII       BlnI        
BlpI        Bpu1102I    BsaWI       BseAI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MfeI        MroI        MunI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI          238  GTCTAC               GT^[AC][GT]AC
AflII        1849  CTTAAG               C^TTAAG
AhdI          181  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1711  GTGCAC               G^TGCAC
ApaLI        1711  GTGCAC               G^TGCAC
Asp700I      2472  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         181  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI         1184  GCATCTTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1199  CTAG                 C^TAG
BfrI         1849  CTTAAG               C^TTAAG
BsaBI         318  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI        1451  ACCTGC               ACCTGC(4/8)
BsrBI        2146  GAGCGG               CCGCTC(-3/-3)
BsrDI        2607  CATTGC               GCAATG(2/0)
BstXI        2399  CCAGCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI         1624  GACGTGCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      181  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1124  CCTCGCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI         1199  CTAG                 C^TAG
MamI          318  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI          233  CCATGG               C^CATGG
NdeI         1997  CATATG               CA^TATG
PshAI         693  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         2416  CTGCAG               CTGCA^G
PvuII         997  CAGCTG               CAG^CTG
ScaI         2231  AGTACT               AGT^ACT
SfcI         2416  CTGCAG               C^T[GA][CT]AG
XcmI         1505  CCAAGGGGCGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2472  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Aor51HI       502  AGCGCT               AGC^GCT
              773  AGCGCT              
AspHI        1711  GTGCAC               G[AT]GC[AT]^C
             2203  GTGCTC              
AspI          378  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2341  GACTCTGTC           
BalI          230  TGGCCA               TGG^CCA
             1532  TGGCCA              
BbrPI        1248  CACGTG               CAC^GTG
             1597  CACGTG              
BsaI          343  GAGACC               GGTCTC(1/5)
             1756  GAGACC              
BsiHKAI      1711  GTGCAC               G[AT]GC[AT]^C
             2203  GTGCTC              
BsmBI         376  GAGACG               CGTCTC(1/5)
             1369  GAGACG              
Bsp1407I      767  TGTACA               T^GTACA
             2714  TGTACA              
BspLU11I      242  ACATGT               A^CATGT
              515  ACATGT              
BsrGI         767  TGTACA               T^GTACA
             2714  TGTACA              
EagI         1142  CGGCCG               C^GGCCG
             2632  CGGCCG              
EclXI        1142  CGGCCG               C^GGCCG
             2632  CGGCCG              
Eco47III      502  AGCGCT               AGC^GCT
              773  AGCGCT              
Eco52I       1142  CGGCCG               C^GGCCG
             2632  CGGCCG              
MluI         1724  ACGCGT               A^CGCGT
             2435  ACGCGT              
MluNI         230  TGGCCA               TGG^CCA
             1532  TGGCCA              
MscI          230  TGGCCA               TGG^CCA
             1532  TGGCCA              
MseI         1850  TTAA                 T^TAA
             2006  TTAA                
PflFI         378  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2341  GACTCTGTC           
PflMI        1209  CCACTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1951  CCAGTTCTTGG         
PmaCI        1248  CACGTG               CAC^GTG
             1597  CACGTG              
PmlI         1248  CACGTG               CAC^GTG
             1597  CACGTG              
SmaI          897  CCCGGG               CCC^GGG
             2138  CCCGGG              
SspBI         767  TGTACA               T^GTACA
             2714  TGTACA              
Tru9I        1850  TTAA                 T^TAA
             2006  TTAA                
Tth111I       378  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2341  GACTCTGTC           
Van91I       1209  CCACTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1951  CCAGTTCTTGG         
XmaCI         897  CCCGGG               C^CCGGG
             2138  CCCGGG              
XmaI          897  CCCGGG               C^CCGGG
             2138  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        ApaI        AvaI        AvaII       
AviII       BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaJI       BseRI       
BsgI        BsiEI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssHII      BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PleI        PpuMI       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfoI        
SmlI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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