Restriction map for pF1KSDA1087
 (Linear) (Six-base) MAPSORT of: KIAA1087.seq  Check: 2259  from: 1  to: 2763



KIAA1087.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AccI GT'mk_AC

Cuts at:      0     239    2763 
   Size:        239    2524
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1756    2763 
   Size:       1756    1007
 
AfeI AGC'GCT

Cuts at:      0     504     775    2763 
   Size:        504     271    1988

  Fragments arranged by size: 

                1988     504     271
 
AflII C'TTAA_G

Cuts at:      0    1849    2763 
   Size:       1849     914
 
AflIII A'CryG_T

Cuts at:      0     242     515     764    1724    2390    2435    2474    2763 
   Size:        242     273     249     960     666      45      39     289

  Fragments arranged by size: 

                 960     666     289     273     249     242      45      39
            
 
AhdI GACnn_n'nnGTC

Cuts at:      0     186    2763 
   Size:        186    2577
 
AleI CACnn'nnGTG

Cuts at:      0     493     856    1351    2763 
   Size:        493     363     495    1412

  Fragments arranged by size: 

                1412     495     493     363
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2629    2763 
   Size:       2629     134
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     838     870    1213    1245    2763 
   Size:        838      32     343      32    1518

  Fragments arranged by size: 

                1518     838     343      32      32
 
ApaI G_GGCC'C

Cuts at:      0     484     898     904    2763 
   Size:        484     414       6    1859

  Fragments arranged by size: 

                1859     484     414       6
 
ApaLI G'TGCA_C

Cuts at:      0    1711    2763 
   Size:       1711    1052
 
AvaI C'yCGr_G

Cuts at:      0     897    1644    2138    2763 
   Size:        897     747     494     625

  Fragments arranged by size: 

                 897     747     625     494
 
BanI G'GyrC_C

Cuts at:      0     355     487     811     862    1087    1137    1174    1521 
   Size:        355     132     324      51     225      50      37     347

Cuts at:   1521    1615    1657    2374    2627    2763 
   Size:         94      42     717     253     136

  Fragments arranged by size: 

                 717     355     347     324     253     225     136     132
                  94      51      50      42      37
 
BanII G_rGCy'C

Cuts at:      0     179     412     484     898     904    1324    2763 
   Size:        179     233      72     414       6     420    1439

  Fragments arranged by size: 

                1439     420     414     233     179      72       6
 
BbeI G_GCGC'C

Cuts at:      0     815     866    1091    1141    1178    1525    2631    2763 
   Size:        815      51     225      50      37     347    1106     132

  Fragments arranged by size: 

                1106     815     347     225     132      51      50      37
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     582     693    1172    2352    2583    2763 
   Size:        582     111     479    1180     231     180

  Fragments arranged by size: 

                1180     582     479     231     180     111
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1173    1207    2763 
   Size:       1173      34    1556

  Fragments arranged by size: 

                1556    1173      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     733    1259    1509    1845    2763 
   Size:        733     526     250     336     918

  Fragments arranged by size: 

                 918     733     526     336     250
 
Bme1580I G_kGCm'C

Cuts at:      0     358     484     898     904    1348    1618    1715    2222 
   Size:        358     126     414       6     444     270      97     507

Cuts at:   2222    2377    2763 
   Size:        155     386

  Fragments arranged by size: 

                 507     444     414     386     358     270     155     126
                  97       6
 
BmgBI CAC'GTC

Cuts at:      0     302    1626    2763 
   Size:        302    1324    1137

  Fragments arranged by size: 

                1324    1137     302
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1836    2529    2763 
   Size:       1836     693     234

  Fragments arranged by size: 

                1836     693     234
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1323    2763 
   Size:       1323    1440
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1012    1237    1759    1996    2755    2763 
   Size:       1012     225     522     237     759       8

  Fragments arranged by size: 

                1012     759     522     237     225       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     940     949    2320    2763 
   Size:        940       9    1371     443

  Fragments arranged by size: 

                1371     940     443       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     337    1750    2763 
   Size:        337    1413    1013

  Fragments arranged by size: 

                1413    1013     337
 
BsaAI yAC'GTr

Cuts at:      0     765    1250    1284    1599    2172    2763 
   Size:        765     485      34     315     573     591

  Fragments arranged by size: 

                 765     591     573     485     315      34
 
BsaBI GATnn'nnATC

Cuts at:      0     322    2763 
   Size:        322    2441
 
BsaHI Gr'CG_yC

Cuts at:      0     812     863     926    1088    1121    1138    1175    1522 
   Size:        812      51      63     162      33      17      37     347

Cuts at:   1522    2492    2628    2763 
   Size:        970     136     135

  Fragments arranged by size: 

                 970     812     347     162     136     135      63      51
                  37      33      17
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1213    1243    2119    2149    2293    2323    2763 
   Size:       1213      30     876      30     144      30     440

  Fragments arranged by size: 

                1213     876     440     144      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     402    1432    1927    1930    1933    2005    2131 
   Size:         22     380    1030     495       3       3      72     126

Cuts at:   2131    2134    2137    2140    2158    2763 
   Size:          3       3       3      18     605

  Fragments arranged by size: 

                1030     605     495     380     126      72      22      18
                   3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     192     477     552     882    1830    2243    2652    2763 
   Size:        192     285      75     330     948     413     409     111

  Fragments arranged by size: 

                 948     413     409     330     285     192     111      75
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1015    1579    2307    2548    2763 
   Size:       1015     564     728     241     215

  Fragments arranged by size: 

                1015     728     564     241     215
 
BsiEI CG_ry'CG

Cuts at:      0     273    1145    2635    2763 
   Size:        273     872    1490     128

  Fragments arranged by size: 

                1490     872     273     128
 
BsiHKAI G_wGCw'C

Cuts at:      0    1715    2207    2763 
   Size:       1715     492     556

  Fragments arranged by size: 

                1715     556     492
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     370    1363    2763 
   Size:        370     993    1400

  Fragments arranged by size: 

                1400     993     370
 
Bsp1286I G_dGCh'C

Cuts at:      0     179     358     412     484     898     904    1324    1348 
   Size:        179     179      54      72     414       6     420      24

Cuts at:   1348    1618    1715    2207    2222    2377    2763 
   Size:        270      97     492      15     155     386

  Fragments arranged by size: 

                 492     420     414     386     270     179     179     155
                  97      72      54      24      15       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1460    2763 
   Size:       1460    1303
 
BsrBI CCG'CTC

Cuts at:      0    2148    2763 
   Size:       2148     615
 
BsrDI GCAATG_nn'

Cuts at:      0    2606    2763 
   Size:       2606     157
 
BsrFI r'CCGG_y

Cuts at:      0     556    1084    1140    1970    2455    2624    2630    2763 
   Size:        556     528      56     830     485     169       6     133

  Fragments arranged by size: 

                 830     556     528     485     169     133      56       6
            
 
BsrGI T'GTAC_A

Cuts at:      0     767    2714    2763 
   Size:        767    1947      49

  Fragments arranged by size: 

                1947     767      49
 
BssHII G'CGCG_C

Cuts at:      0     913    1649    1651    1696    2763 
   Size:        913     736       2      45    1067

  Fragments arranged by size: 

                1067     913     736      45       2
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2406    2763 
   Size:       2406     357
 
BstYI r'GATC_y

Cuts at:      0     425     950     987    2763 
   Size:        425     525      37    1776

  Fragments arranged by size: 

                1776     525     425      37
 
BtgI C'CryG_G

Cuts at:      0     233    1619    2238    2763 
   Size:        233    1386     619     525

  Fragments arranged by size: 

                1386     619     525     233
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     249     513     993    2763 
   Size:        249     264     480    1770

  Fragments arranged by size: 

                1770     480     264     249
 
BtsI GCAGTG_nn'

Cuts at:      0     221     599    1209    2763 
   Size:        221     378     610    1554

  Fragments arranged by size: 

                1554     610     378     221
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1630    2763 
   Size:       1630    1133
 
EaeI y'GGCC_r

Cuts at:      0      95     230     284     453     734    1142    1532    2507 
   Size:         95     135      54     169     281     408     390     975

Cuts at:   2507    2632    2763 
   Size:        125     131

  Fragments arranged by size: 

                 975     408     390     281     169     135     131     125
                  95      54
 
EagI C'GGCC_G

Cuts at:      0    1142    2632    2763 
   Size:       1142    1490     131

  Fragments arranged by size: 

                1490    1142     131
 
EarI CTCTTCn'nnn_

Cuts at:      0     757     826    1036    1775    2212    2587    2698    2728 
   Size:        757      69     210     739     437     375     111      30

Cuts at:   2728    2763 
   Size:         35

  Fragments arranged by size: 

                 757     739     437     375     210     111      69      35
                  30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1132    2763 
   Size:       1132    1631
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1012    1237    1756    1759    1996    2755    2763 
   Size:       1012     225     519       3     237     759       8

  Fragments arranged by size: 

                1012     759     519     237     225       8       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1128    2763 
   Size:       1128    1635
 
EcoO109I rG'GnC_Cy

Cuts at:      0     134     870     901     960    2060    2135    2301    2763 
   Size:        134     736      31      59    1100      75     166     462

  Fragments arranged by size: 

                1100     736     462     166     134      75      59      31
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2223    2763 
   Size:       2223     540
 
FspI TGC'GCA

Cuts at:      0    1105    1387    1639    2551    2763 
   Size:       1105     282     252     912     212

  Fragments arranged by size: 

                1105     912     282     252     212
 
FspAI rTGC'GCAy

Cuts at:      0    2551    2763 
   Size:       2551     212
 
HaeII r_GCGC'y

Cuts at:      0     506     688     777     815     866    1091    1141    1178 
   Size:        506     182      89      38      51     225      50      37

Cuts at:   1178    1525    2416    2631    2763 
   Size:        347     891     215     132

  Fragments arranged by size: 

                 891     506     347     225     215     182     132      89
                  51      50      38      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     245     277     367     399    1851    1883 
   Size:        172      32      41      32      90      32    1452      32

Cuts at:   1883    2119    2151    2293    2325    2660    2692    2763 
   Size:        236      32     142      32     335      32      71

  Fragments arranged by size: 

                1452     335     236     172     142      90      71      41
                  32      32      32      32      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     811     862    1087    1137    1174    1521    2627    2763 
   Size:        811      51     225      50      37     347    1106     136

  Fragments arranged by size: 

                1106     811     347     225     136      51      50      37
            
 
MluI A'CGCG_T

Cuts at:      0    1724    2435    2763 
   Size:       1724     711     328

  Fragments arranged by size: 

                1724     711     328
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     410    1349    2486    2763 
   Size:        410     939    1137     277

  Fragments arranged by size: 

                1137     939     410     277
 
MscI TGG'CCA

Cuts at:      0     232    1534    2763 
   Size:        232    1302    1229

  Fragments arranged by size: 

                1302    1229     232
 
MslI CAynn'nnrTG

Cuts at:      0     397     493     520     532     856    1351    2611    2763 
   Size:        397      96      27      12     324     495    1260     152

  Fragments arranged by size: 

                1260     495     397     324     152      96      27      12
            
 
MspA1I CmG'CkG

Cuts at:      0     143     797     999    1527    2763 
   Size:        143     654     202     528    1236

  Fragments arranged by size: 

                1236     654     528     202     143
 
NaeI GCC'GGC

Cuts at:      0     558    1142    1972    2632    2763 
   Size:        558     584     830     660     131

  Fragments arranged by size: 

                 830     660     584     558     131
 
NarI GG'CG_CC

Cuts at:      0     812     863    1088    1138    1175    1522    2628    2763 
   Size:        812      51     225      50      37     347    1106     135

  Fragments arranged by size: 

                1106     812     347     225     135      51      50      37
            
 
NcoI C'CATG_G

Cuts at:      0     233    2763 
   Size:        233    2530
 
NdeI CA'TA_TG

Cuts at:      0    1998    2763 
   Size:       1998     765
 
NgoMIV G'CCGG_C

Cuts at:      0     556    1140    1970    2630    2763 
   Size:        556     584     830     660     133

  Fragments arranged by size: 

                 830     660     584     556     133
 
NspI r_CATG'y

Cuts at:      0     246     519    1479    2763 
   Size:        246     273     960    1284

  Fragments arranged by size: 

                1284     960     273     246
 
PasI CC'CwG_GG

Cuts at:      0     405    2370    2763 
   Size:        405    1965     393

  Fragments arranged by size: 

                1965     405     393
 
PciI A'CATG_T

Cuts at:      0     242     515    2763 
   Size:        242     273    2248

  Fragments arranged by size: 

                2248     273     242
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1215    1957    2763 
   Size:       1215     742     806

  Fragments arranged by size: 

                1215     806     742
 
PfoI T'CCnGG_A

Cuts at:      0    1547    1580    2763 
   Size:       1547      33    1183

  Fragments arranged by size: 

                1547    1183      33
 
PmlI CAC'GTG

Cuts at:      0    1250    1599    2763 
   Size:       1250     349    1164

  Fragments arranged by size: 

                1250    1164     349
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1213    1245    2763 
   Size:       1213      32    1518

  Fragments arranged by size: 

                1518    1213      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     134     960    2060    2135    2301    2763 
   Size:        134     826    1100      75     166     462

  Fragments arranged by size: 

                1100     826     462     166     134      75
 
PshAI GACnn'nnGTC

Cuts at:      0     697    2763 
   Size:        697    2066
 
PspOMI G'GGCC_C

Cuts at:      0     480     894     900    2763 
   Size:        480     414       6    1863

  Fragments arranged by size: 

                1863     480     414       6
 
PstI C_TGCA'G

Cuts at:      0    2420    2763 
   Size:       2420     343
 
PvuII CAG'CTG

Cuts at:      0     999    2763 
   Size:        999    1764
 
SanDI GG'GwC_CC

Cuts at:      0    2060    2135    2763 
   Size:       2060      75     628

  Fragments arranged by size: 

                2060     628      75
 
SapI GCTCTTCn'nnn_

Cuts at:      0     757     826    1036    2212    2587    2698    2763 
   Size:        757      69     210    1176     375     111      65

  Fragments arranged by size: 

                1176     757     375     210     111      69      65
 
ScaI AGT'ACT

Cuts at:      0    2233    2763 
   Size:       2233     530
 
SfcI C'TryA_G

Cuts at:      0    2416    2763 
   Size:       2416     347
 
SfoI GGC'GCC

Cuts at:      0     813     864    1089    1139    1176    1523    2629    2763 
   Size:        813      51     225      50      37     347    1106     134

  Fragments arranged by size: 

                1106     813     347     225     134      51      50      37
            
 
SmaI CCC'GGG

Cuts at:      0     899    2140    2763 
   Size:        899    1241     623

  Fragments arranged by size: 

                1241     899     623
 
SmlI C'TyrA_G

Cuts at:      0     955     964    1849    2335    2763 
   Size:        955       9     885     486     428

  Fragments arranged by size: 

                 955     885     486     428       9
 
SrfI GCCC'GGGC

Cuts at:      0     899    2763 
   Size:        899    1864
 
StyI C'CwwG_G

Cuts at:      0      14     233     329    1313    1505    2048    2481    2763 
   Size:         14     219      96     984     192     543     433     282

  Fragments arranged by size: 

                 984     543     433     282     219     192      96      14
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184    2763 
   Size:        184    2579
 
TatI w'GTAC_w

Cuts at:      0     221     767    1334    2231    2516    2714    2763 
   Size:        221     546     567     897     285     198      49

  Fragments arranged by size: 

                 897     567     546     285     221     198      49
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     410     442    2763 
   Size:        410      32    2321

  Fragments arranged by size: 

                2321     410      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     381    2344    2763 
   Size:        381    1963     419

  Fragments arranged by size: 

                1963     419     381
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1512    2763 
   Size:       1512    1251
 
XmaI C'CCGG_G

Cuts at:      0     897    2138    2763 
   Size:        897    1241     625

  Fragments arranged by size: 

                1241     897     625
 
XmnI GAAnn'nnTTC

Cuts at:      0    2476    2763 
   Size:       2476     287
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII   AflIII     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AvaI     BanI    BanII     BbeI     BbsI
     BcgI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BstXI    BstYI     BtgI    BtgZI     BtsI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    FspAI    HaeII    Hin4I     KasI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI
   PspOMI     PstI    PvuII    SanDI     SapI     ScaI     SfcI     SfoI
     SmaI     SmlI     SrfI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AgeI    AlwNI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI
    BfrBI    BglII     BlpI     BmtI   Bpu10I    BsaWI    BsiWI     BsmI
    BspEI    BspHI    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII  EcoICRI    EcoRI    EcoRV     FseI
   HincII  HindIII     HpaI     KpnI     MfeI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SbfI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     SwaI     XbaI     XhoI
     ZraI
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