Restriction map for pF1KSDA1074
 (Linear) (Six-base) MAPSORT of: KIAA1074.seq  Check: 4628  from: 1  to: 5127



KIAA1074.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AccI GT'mk_AC

Cuts at:      0    4257    5041    5127 
   Size:       4257     784      86

  Fragments arranged by size: 

                4257     784      86
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     743    1736    2848    3925    3992    5127 
   Size:        743     993    1112    1077      67    1135

  Fragments arranged by size: 

                1135    1112    1077     993     743      67
 
AflII C'TTAA_G

Cuts at:      0     732    5127 
   Size:        732    4395
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2489    2521    3080    3112    5127 
   Size:       2489      32     559      32    2015

  Fragments arranged by size: 

                2489    2015     559      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3835    4020    5127 
   Size:       3835     185    1107

  Fragments arranged by size: 

                3835    1107     185
 
ApaLI G'TGCA_C

Cuts at:      0    1186    5127 
   Size:       1186    3941
 
ApoI r'AATT_y

Cuts at:      0     586     870    1281    1884    2115    2627    2685    2694 
   Size:        586     284     411     603     231     512      58       9

Cuts at:   2694    2961    3141    3963    4138    4280    5127 
   Size:        267     180     822     175     142     847

  Fragments arranged by size: 

                 847     822     603     586     512     411     284     267
                 231     180     175     142      58       9
 
AseI AT'TA_AT

Cuts at:      0    1741    3491    3746    4193    5127 
   Size:       1741    1750     255     447     934

  Fragments arranged by size: 

                1750    1741     934     447     255
 
AvaI C'yCGr_G

Cuts at:      0    2594    5127 
   Size:       2594    2533
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3322    3355    5127 
   Size:       3322      33    1772

  Fragments arranged by size: 

                3322    1772      33
 
BanI G'GyrC_C

Cuts at:      0      97    5127 
   Size:         97    5030
 
BanII G_rGCy'C

Cuts at:      0      43    5127 
   Size:         43    5084
 
BbeI G_GCGC'C

Cuts at:      0     101    5127 
   Size:        101    5026
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     731     797    2273    2864    2875    3487    3547    4637 
   Size:        731      66    1476     591      11     612      60    1090

Cuts at:   4637    5127 
   Size:        490

  Fragments arranged by size: 

                1476    1090     731     612     591     490      66      60
                  11
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2342    2376    2575    2609    5127 
   Size:       2342      34     199      34    2518

  Fragments arranged by size: 

                2518    2342     199      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     668    1419    4260    5127 
   Size:        668     751    2841     867

  Fragments arranged by size: 

                2841     867     751     668
 
BclI T'GATC_A

Cuts at:      0    1770    3057    4370    5127 
   Size:       1770    1287    1313     757

  Fragments arranged by size: 

                1770    1313    1287     757
 
BglII A'GATC_T

Cuts at:      0     135    4590    4780    4854    5067    5127 
   Size:        135    4455     190      74     213      60

  Fragments arranged by size: 

                4455     213     190     135      74      60
 
BlpI GC'TnA_GC

Cuts at:      0     831    5127 
   Size:        831    4296
 
Bme1580I G_kGCm'C

Cuts at:      0    1190    5127 
   Size:       1190    3937
 
BmgBI CAC'GTC

Cuts at:      0     123    5127 
   Size:        123    5004
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2907    5127 
   Size:       2907    2220
 
BmtI G_CTAG'C

Cuts at:      0    4738    5127 
   Size:       4738     389
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1290    3271    3422    5127 
   Size:       1290    1981     151    1705

  Fragments arranged by size: 

                1981    1705    1290     151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2536    2992    3742    4255    5127 
   Size:       2536     456     750     513     872

  Fragments arranged by size: 

                2536     872     750     513     456
 
Bpu10I CC'TnA_GC

Cuts at:      0     202    3298    5127 
   Size:        202    3096    1829

  Fragments arranged by size: 

                3096    1829     202
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4502    4610    4962    5127 
   Size:       4502     108     352     165

  Fragments arranged by size: 

                4502     352     165     108
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     122    5127 
   Size:        122    5005
 
BsaBI GATnn'nnATC

Cuts at:      0     416    3056    3493    5127 
   Size:        416    2640     437    1634

  Fragments arranged by size: 

                2640    1634     437     416
 
BsaHI Gr'CG_yC

Cuts at:      0      98    5127 
   Size:         98    5029
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2489    2519    3388    3418    3715    3745    5127 
   Size:       2489      30     869      30     297      30    1382

  Fragments arranged by size: 

                2489    1382     869     297      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75    3409    3949    4306    5127 
   Size:         75    3334     540     357     821

  Fragments arranged by size: 

                3334     821     540     357      75
 
BseYI C'CCAG_C

Cuts at:      0    1312    5127 
   Size:       1312    3815
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205    5127 
   Size:        205    4922
 
BsiHKAI G_wGCw'C

Cuts at:      0    1140    1190    1849    2365    5127 
   Size:       1140      50     659     516    2762

  Fragments arranged by size: 

                2762    1140     659     516      50
 
BsmI GAATG_Cn'

Cuts at:      0    2610    2955    5127 
   Size:       2610     345    2172

  Fragments arranged by size: 

                2610    2172     345
 
Bsp1286I G_dGCh'C

Cuts at:      0      43    1140    1190    1849    2365    5127 
   Size:         43    1097      50     659     516    2762

  Fragments arranged by size: 

                2762    1097     659     516      50      43
 
BspHI T'CATG_A

Cuts at:      0    2706    5127 
   Size:       2706    2421
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    4234    5127 
   Size:       4234     893
 
BsrDI GCAATG_nn'

Cuts at:      0    1097    2960    5127 
   Size:       1097    1863    2167

  Fragments arranged by size: 

                2167    1863    1097
 
BsrGI T'GTAC_A

Cuts at:      0     360    1545    5097    5127 
   Size:        360    1185    3552      30

  Fragments arranged by size: 

                3552    1185     360      30
 
BssSI C'ACGA_G

Cuts at:      0    3243    5127 
   Size:       3243    1884
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2920    5127 
   Size:       2920    2207
 
BstYI r'GATC_y

Cuts at:      0     135     146     191    4590    4780    4854    5067    5127 
   Size:        135      11      45    4399     190      74     213      60

  Fragments arranged by size: 

                4399     213     190     135      74      60      45      11
            
 
BtgI C'CryG_G

Cuts at:      0     432    4149    4909    5127 
   Size:        432    3717     760     218

  Fragments arranged by size: 

                3717     760     432     218
 
BtsI GCAGTG_nn'

Cuts at:      0     440    5127 
   Size:        440    4687
 
DraI TTT'AAA

Cuts at:      0    3198    3773    4160    5108    5127 
   Size:       3198     575     387     948      19

  Fragments arranged by size: 

                3198     948     575     387      19
 
DraIII CAC_nnn'GTG

Cuts at:      0    4828    5127 
   Size:       4828     299
 
EarI CTCTTCn'nnn_

Cuts at:      0     368     452    1589    2387    2392    2450    5127 
   Size:        368      84    1137     798       5      58    2677

  Fragments arranged by size: 

                2677    1137     798     368      84      58       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      72    5127 
   Size:         72    5055
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     743    1736    2536    2848    2992    3742    3925    3992 
   Size:        743     993     800     312     144     750     183      67

Cuts at:   3992    4255    5127 
   Size:        263     872

  Fragments arranged by size: 

                 993     872     800     750     743     312     263     183
                 144      67
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     735    5127 
   Size:        735    4392
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2829    3294    5127 
   Size:       2829     465    1833

  Fragments arranged by size: 

                2829    1833     465
 
EcoRI G'AATT_C

Cuts at:      0    1884    2627    2685    2694    5127 
   Size:       1884     743      58       9    2433

  Fragments arranged by size: 

                2433    1884     743      58       9
 
EcoRV GAT'ATC

Cuts at:      0    1477    5127 
   Size:       1477    3650
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     231    4110    5127 
   Size:        231    3879    1017

  Fragments arranged by size: 

                3879    1017     231
 
HaeII r_GCGC'y

Cuts at:      0     101    5127 
   Size:        101    5026
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     460     492     703     735     837     869    1198    1230 
   Size:        460      32     211      32     102      32     329      32

Cuts at:   1230    1234    1266    3168    3200    3388    3420    3715    3747 
   Size:          4      32    1902      32     188      32     295      32

Cuts at:   3747    3846    3878    4203    4235    4285    4317    4582    4614 
   Size:         99      32     325      32      50      32     265      32

Cuts at:   4614    5127 
   Size:        513

  Fragments arranged by size: 

                1902     513     460     329     325     295     265     211
                 188     102      99      50      32      32      32      32
                  32      32      32      32      32      32      32      32
                   4
 
HincII GTy'rAC

Cuts at:      0    4746    5127 
   Size:       4746     381
 
HindIII A'AGCT_T

Cuts at:      0     828    3874    5127 
   Size:        828    3046    1253

  Fragments arranged by size: 

                3046    1253     828
 
KasI G'GCGC_C

Cuts at:      0      97    5127 
   Size:         97    5030
 
MfeI C'AATT_G

Cuts at:      0    3802    5127 
   Size:       3802    1325
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     611    4337    4943    5127 
   Size:        611    3726     606     184

  Fragments arranged by size: 

                3726     611     606     184
 
MslI CAynn'nnrTG

Cuts at:      0    1501    1697    2705    3053    4023    5127 
   Size:       1501     196    1008     348     970    1104

  Fragments arranged by size: 

                1501    1104    1008     970     348     196
 
MspA1I CmG'CkG

Cuts at:      0    1564    2944    5127 
   Size:       1564    1380    2183

  Fragments arranged by size: 

                2183    1564    1380
 
NarI GG'CG_CC

Cuts at:      0      98    5127 
   Size:         98    5029
 
NcoI C'CATG_G

Cuts at:      0     432    4149    5127 
   Size:        432    3717     978

  Fragments arranged by size: 

                3717     978     432
 
NdeI CA'TA_TG

Cuts at:      0     885    5127 
   Size:        885    4242
 
NheI G'CTAG_C

Cuts at:      0    4734    5127 
   Size:       4734     393
 
NspI r_CATG'y

Cuts at:      0    1446    2745    5127 
   Size:       1446    1299    2382

  Fragments arranged by size: 

                2382    1446    1299
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1897    2514    5127 
   Size:       1897     617    2613

  Fragments arranged by size: 

                2613    1897     617
 
PfoI T'CCnGG_A

Cuts at:      0     862    2180    5127 
   Size:        862    1318    2947

  Fragments arranged by size: 

                2947    1318     862
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2489    2521    5127 
   Size:       2489      32    2606

  Fragments arranged by size: 

                2606    2489      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2829    3294    5127 
   Size:       2829     465    1833

  Fragments arranged by size: 

                2829    1833     465
 
PstI C_TGCA'G

Cuts at:      0    1323    4245    5127 
   Size:       1323    2922     882

  Fragments arranged by size: 

                2922    1323     882
 
PvuII CAG'CTG

Cuts at:      0    1564    2944    5127 
   Size:       1564    1380    2183

  Fragments arranged by size: 

                2183    1564    1380
 
SapI GCTCTTCn'nnn_

Cuts at:      0     452    1589    2450    5127 
   Size:        452    1137     861    2677

  Fragments arranged by size: 

                2677    1137     861     452
 
SfcI C'TryA_G

Cuts at:      0    1319    3389    4241    4336    5127 
   Size:       1319    2070     852      95     791

  Fragments arranged by size: 

                2070    1319     852     791      95
 
SfoI GGC'GCC

Cuts at:      0      99    5127 
   Size:         99    5028
 
SmlI C'TyrA_G

Cuts at:      0     732    2594    4517    4625    4941    5127 
   Size:        732    1862    1923     108     316     186

  Fragments arranged by size: 

                1923    1862     732     316     186     108
 
SpeI A'CTAG_T

Cuts at:      0    4165    5127 
   Size:       4165     962
 
SphI G_CATG'C

Cuts at:      0    1446    5127 
   Size:       1446    3681
 
SspI AAT'ATT

Cuts at:      0     513    5127 
   Size:        513    4614
 
StuI AGG'CCT

Cuts at:      0    1983    5127 
   Size:       1983    3144
 
StyI C'CwwG_G

Cuts at:      0      35     432    1255    4149    5127 
   Size:         35     397     823    2894     978

  Fragments arranged by size: 

                2894     978     823     397      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     146    5127 
   Size:        146    4981
 
TatI w'GTAC_w

Cuts at:      0     360    1545    2280    4314    5097    5127 
   Size:        360    1185     735    2034     783      30

  Fragments arranged by size: 

                2034    1185     783     735     360      30
 
XbaI T'CTAG_A

Cuts at:      0    3028    4674    5127 
   Size:       3028    1646     453

  Fragments arranged by size: 

                3028    1646     453
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4758    4812    5127 
   Size:       4758      54     315

  Fragments arranged by size: 

                4758     315      54
 
XhoI C'TCGA_G

Cuts at:      0    2594    5127 
   Size:       2594    2533
 
XmnI GAAnn'nnTTC

Cuts at:      0     965    2861    5127 
   Size:        965    1896    2266

  Fragments arranged by size: 

                2266    1896     965
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AloI    AlwNI    ApaLI     ApoI     AseI
     AvaI     BaeI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI    BssSI    BstXI    BstYI     BtgI     BtsI     DraI   DraIII
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
    HaeII    Hin4I   HincII  HindIII     KasI     MfeI     MmeI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NspI    PflMI     PfoI
     PpiI    PpuMI     PstI    PvuII     SapI     SfcI     SfoI     SmlI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     XbaI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI     AscI    AsiSI    AvrII    BamHI    BbvCI
    BfrBI     BglI    BsaAI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DrdI     EaeI     EagI  EcoICRI     FseI
     FspI    FspAI     HpaI     KpnI     MluI     MscI     NaeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SrfI     SwaI     TstI  Tth111I     XmaI
     ZraI
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