Restriction map for pF1KSDA1072
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AgeI        AhdI        Aor51HI     
ApaI        ApoI        AscI        Asp718I     AspEI       
AspI        BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspMI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        DraI        DraIII      Eam1105I    Eco47III    
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HgaI        Hin1I       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tth111I     TthHB8I     
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AfaI         2193  GTAC                 GT^AC
AflII         981  CTTAAG               C^TTAAG
Alw44I       1790  GTGCAC               G^TGCAC
AlwI          907  GATCC                GGATC(4/5)
AlwNI        1253  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1790  GTGCAC               G^TGCAC
AseI         1813  ATTAAT               AT^TAAT
AsnI         1813  ATTAAT               AT^TAAT
AviII         725  TGCGCA               TGC^GCA
AvrII        1623  CCTAGG               C^CTAGG
BalI          825  TGGCCA               TGG^CCA
BanI         1171  GGTGCC               G^G[CT][GA]CC
BfrI          981  CTTAAG               C^TTAAG
BglI         2166  GCCATGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1623  CCTAGG               C^CTAGG
BlpI         1607  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1607  GCTCAGC              GC^T[ACGT]AGC
BsaI          678  GAGACC               GGTCTC(1/5)
BspHI        2226  TCATGA               T^CATGA
BsrDI        1853  CATTGC               GCAATG(2/0)
BstAPI       1252  GCAGATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1607  GCTCAGC              GC^T[ACGT]AGC
CpoI           69  CGGACCG              CG^G[AT]CCG
DrdI          742  GACAAACATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI          725  TGCGCA               TGC^GCA
HaeII          35  GGCGCT               [GA]GCGC^[CT]
HincII        874  GTTAAC               GT[CT]^[GA]AC
HindII        874  GTTAAC               GT[CT]^[GA]AC
HindIII       803  AAGCTT               A^AGCTT
HpaI          874  GTTAAC               GTT^AAC
MluNI         825  TGGCCA               TGG^CCA
MscI          825  TGGCCA               TGG^CCA
MslI          996  CACAGGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI            4  GCCGGC               GCC^GGC
NgoMIV          4  GCCGGC               G^CCGGC
PpuMI        1678  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         392  GACCGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1813  ATTAAT               AT^TAAT
RcaI         2226  TCATGA               T^CATGA
RsaI         2193  GTAC                 GT^AC
RsrII          69  CGGACCG              CG^G[AT]CCG
SapI          774  GCTCTTC              GCTCTTC(1/4)
XbaI         1999  TCTAGA               T^CTAGA
XcmI         2242  CCAGATCTTCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AflIII        747  ACATGT               A^C[GA][CT]GT
             1032  ACATGT              
AocI           16  CCTGAGG              CC^T[ACGT]AGG
             1467  CCTGAGG             
Asp700I       520  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              657  GAAAGACTTC          
AspHI        1605  GTGCTC               G[AT]GC[AT]^C
             1790  GTGCAC              
BglII        1716  AGATCT               A^GATCT
             2244  AGATCT              
BsiHKAI      1605  GTGCTC               G[AT]GC[AT]^C
             1790  GTGCAC              
BsmI         1928  GAATGC               GAATGC(1/-1)
              755  GCATTC              
BspLU11I      747  ACATGT               A^CATGT
             1032  ACATGT              
BsrBI         382  CCGCTC               CCGCTC(-3/-3)
              949  GAGCGG              
BsrFI           4  GCCGGC               [GA]^CCGG[CT]
               32  ACCGGC              
BssSI         634  CACGAG               CACGAG(-5/-1)
             1421  CTCGTG              
BstXI         995  CCACAGGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1265  CCACTGTTCTGG        
BstYI        1716  AGATCT               [GA]^GATC[CT]
             2244  AGATCT              
Bsu36I         16  CCTGAGG              CC^T[ACGT]AGG
             1467  CCTGAGG             
Cfr10I          4  GCCGGC               [GA]^CCGG[CT]
               32  ACCGGC              
EagI          179  CGGCCG               C^GGCCG
              341  CGGCCG              
EarI          775  CTCTTC               CTCTTC(1/4)
             2139  GAAGAG              
EclXI         179  CGGCCG               C^GGCCG
              341  CGGCCG              
Eco52I        179  CGGCCG               C^GGCCG
              341  CGGCCG              
Eco81I         16  CCTGAGG              CC^T[ACGT]AGG
             1467  CCTGAGG             
Ksp632I       775  CTCTTC               CTCTTC(1/4)
             2139  GAAGAG              
MaeII         872  ACGT                 A^CGT
             1691  ACGT                
MaeIII        940  GTCAC                ^GT[ACGT]AC
             1006  GTGAC               
MflI         1716  AGATCT               [GA]^GATC[CT]
             2244  AGATCT              
NcoI          828  CCATGG               C^CATGG
             2167  CCATGG              
PleI         2223  GACTC                GAGTC(4/5)
              136  GAGTC               
PstI          451  CTGCAG               CTGCA^G
              645  CTGCAG              
PvuII         364  CAGCTG               CAG^CTG
              648  CAGCTG              
SfcI          451  CTGCAG               C^T[GA][CT]AG
              645  CTGCAG              
SmlI          981  CTTAAG               C^T[CT][GA]AG
             2084  CTCAAG              
Tsp45I        940  GTCAC                ^GT[GC]AC
             1006  GTGAC               
XhoII        1716  AGATCT               [GA]^GATC[CT]
             2244  AGATCT              
XmnI          520  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              657  GAAAGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        AvaII       
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiEI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MboI        MboII       MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmaI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XmaCI       XmaI        
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