Restriction map for pF1KSDA1067
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        AocI        AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BamHI       BbrPI       BbvCI       BciVI       
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspEI       BspLU11I    BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      Cfr10I      CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          652  GTCTAC               GT^[AC][GT]AC
AcsI          457  GAATTT               [GA]^AATT[CT]
AcyI         1408  GGCGCC               G[GA]^CG[CT]C
Alw44I        187  GTGCAC               G^TGCAC
ApaI          309  GGGCCC               GGGCC^C
ApaLI         187  GTGCAC               G^TGCAC
ApoI          457  GAATTT               [GA]^AATT[CT]
Asp700I      1023  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI         1408  GGCGCC               GGCGC^C
BcgI         2040  CGACACCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          518  TGATCA               T^GATCA
BfaI          859  CTAG                 C^TAG
BsaHI        1408  GGCGCC               G[GA]^CG[CT]C
BsgI         1576  CTGCAC               GTGCAG(16/14)
BsiEI        2028  CGATCG               CG[GA][CT]^CG
BspDI        2026  ATCGAT               AT^CGAT
BspHI         644  TCATGA               T^CATGA
BsrDI        1358  GCAATG               GCAATG(2/0)
BssSI        1345  CACGAG               CACGAG(-5/-1)
BstAPI        210  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI         2026  ATCGAT               AT^CGAT
FbaI          518  TGATCA               T^GATCA
Hin1I        1408  GGCGCC               G[GA]^CG[CT]C
KasI         1408  GGCGCC               G^GCGCC
MaeI          859  CTAG                 C^TAG
NarI         1408  GGCGCC               GG^CGCC
NcoI         1247  CCATGG               C^CATGG
PpuMI        1550  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1914  GACCATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         2028  CGATCG               CGAT^CG
RcaI          644  TCATGA               T^CATGA
SacI         1348  GAGCTC               GAGCT^C
SexAI        1187  ACCAGGT              A^CC[AT]GGT
SfoI         1408  GGCGCC               GGC^GCC
StuI         1859  AGGCCT               AGG^CCT
XbaI          858  TCTAGA               T^CTAGA
XcmI          314  CCACAGGTAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1023  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII         433  CGCG                 CG^CG
              476  CGCG                
AlwNI         211  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              985  CAGCTGCTG           
Aor51HI      1269  AGCGCT               AGC^GCT
             1661  AGCGCT              
AvaI          574  CCCGAG               C^[CT]CG[GA]G
             1006  CCCGAG              
BalI          278  TGGCCA               TGG^CCA
              347  TGGCCA              
BglI         1096  GCCCGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1411  GCCATGCTGGC         
BseRI          61  GAGGAG               GAGGAG(10/8)
               64  GAGGAG              
BsmBI         492  CGTCTC               CGTCTC(1/5)
             1399  GAGACG              
BsoBI         574  CCCGAG               C^[CT]CG[GA]G
             1006  CCCGAG              
BsrI          281  CCAGT                ACTGG(1/-1)
              793  CCAGT               
BstUI         433  CGCG                 CG^CG
              476  CGCG                
BstXI         671  CCAGCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1265  CCAAAGCGCTGG        
BstYI         513  GGATCT               [GA]^GATC[CT]
             1691  AGATCC              
DsaI         1127  CCACGG               C^C[GA][CT]GG
             1247  CCATGG              
Eco47III     1269  AGCGCT               AGC^GCT
             1661  AGCGCT              
EcoNI         892  CCTCTGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1038  CCTGATACAGG         
EcoT14I      1247  CCATGG               C^C[AT][AT]GG
             1538  CCAAGG              
MflI          513  GGATCT               [GA]^GATC[CT]
             1691  AGATCC              
MluNI         278  TGGCCA               TGG^CCA
              347  TGGCCA              
MscI          278  TGGCCA               TGG^CCA
              347  TGGCCA              
MseI         1717  TTAA                 T^TAA
             1814  TTAA                
MslI          180  CATCCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1242  CACATCCATG          
MvnI          433  CGCG                 CG^CG
              476  CGCG                
NspI          911  ACATGC               [GA]CATG^[CT]
             1322  ACATGC              
PflMI         984  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1629  CCAGCTGGTGG         
SapI           99  GAAGAGC              GCTCTTC(1/4)
             1436  GCTCTTC             
SmlI         1162  CTCAAG               C^T[CT][GA]AG
             1600  CTCAAG              
StyI         1247  CCATGG               C^C[AT][AT]GG
             1538  CCAAGG              
Tru9I        1717  TTAA                 T^TAA
             1814  TTAA                
Tsp509I       458  AATT                 ^AATT
             1591  AATT                
Van91I        984  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1629  CCAGCTGGTGG         
XhoII         513  GGATCT               [GA]^GATC[CT]
             1691  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AspI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrBI       BssKI       BstF5I      BstNI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflFI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
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