Restriction map for pF1KSDA1067
 (Linear) (Six-base) MAPSORT of: KIAA1067.seq  Check: 7327  from: 1  to: 2052



KIAA1067.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     578    2001    2052 
   Size:        578    1423      51

  Fragments arranged by size: 

                1423     578      51
 
AccI GT'mk_AC

Cuts at:      0     653    2052 
   Size:        653    1399
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     721    2052 
   Size:         73     648    1331

  Fragments arranged by size: 

                1331     648      73
 
AfeI AGC'GCT

Cuts at:      0    1271    1663    2052 
   Size:       1271     392     389

  Fragments arranged by size: 

                1271     392     389
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1492    2052 
   Size:       1492     560
 
AlwNI CAG_nnn'CTG

Cuts at:      0     216     990    2052 
   Size:        216     774    1062

  Fragments arranged by size: 

                1062     774     216
 
ApaI G_GGCC'C

Cuts at:      0     313    2052 
   Size:        313    1739
 
ApaLI G'TGCA_C

Cuts at:      0     187    2052 
   Size:        187    1865
 
ApoI r'AATT_y

Cuts at:      0     457    2052 
   Size:        457    1595
 
AvaI C'yCGr_G

Cuts at:      0     574    1006    2052 
   Size:        574     432    1046

  Fragments arranged by size: 

                1046     574     432
 
BanI G'GyrC_C

Cuts at:      0    1261    1336    1408    2052 
   Size:       1261      75      72     644

  Fragments arranged by size: 

                1261     644      75      72
 
BanII G_rGCy'C

Cuts at:      0     313     880    1352    2052 
   Size:        313     567     472     700

  Fragments arranged by size: 

                 700     567     472     313
 
BbeI G_GCGC'C

Cuts at:      0    1412    2052 
   Size:       1412     640
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     241    1030    1134    1657    1918    2052 
   Size:        241     789     104     523     261     134

  Fragments arranged by size: 

                 789     523     261     241     134     104
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2029    2052 
   Size:       2029      23
 
BclI T'GATC_A

Cuts at:      0     518    2052 
   Size:        518    1534
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1102    1417    2052 
   Size:       1102     315     635

  Fragments arranged by size: 

                1102     635     315
 
BlpI GC'TnA_GC

Cuts at:      0    1480    1528    1658    2052 
   Size:       1480      48     130     394

  Fragments arranged by size: 

                1480     394     130      48
 
Bme1580I G_kGCm'C

Cuts at:      0     191     313    1204    1967    2052 
   Size:        191     122     891     763      85

  Fragments arranged by size: 

                 891     763     191     122      85
 
BmgBI CAC'GTC

Cuts at:      0     555     921    2052 
   Size:        555     366    1131

  Fragments arranged by size: 

                1131     555     366
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1452    1792    2052 
   Size:       1452     340     260

  Fragments arranged by size: 

                1452     340     260
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     187     469     559     571     583     654    1180 
   Size:        115      72     282      90      12      12      71     526

Cuts at:   1180    1294    1630    2052 
   Size:        114     336     422

  Fragments arranged by size: 

                 526     422     336     282     115     114      90      72
                  71      12      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1147    1585    2052 
   Size:       1147     438     467

  Fragments arranged by size: 

                1147     467     438
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     919    1246    1423    1921    2052 
   Size:        919     327     177     498     131

  Fragments arranged by size: 

                 919     498     327     177     131
 
BsaHI Gr'CG_yC

Cuts at:      0    1409    2052 
   Size:       1409     643
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      58      88    1108    1138    2052 
   Size:         58      30    1020      30     914

  Fragments arranged by size: 

                1020     914      58      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76      79    2052 
   Size:         76       3    1973

  Fragments arranged by size: 

                1973      76       3
 
BseYI C'CCAG_C

Cuts at:      0     848    1503    1554    2052 
   Size:        848     655      51     498

  Fragments arranged by size: 

                 848     655     498      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1561    2052 
   Size:       1561     491
 
BsiEI CG_ry'CG

Cuts at:      0    2031    2052 
   Size:       2031      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     191     506     569     587    1013    1136    1196    1352 
   Size:        191     315      63      18     426     123      60     156

Cuts at:   1352    1679    2052 
   Size:        327     373

  Fragments arranged by size: 

                 426     373     327     315     191     156     123      63
                  60      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     498    1393    2052 
   Size:        498     895     659

  Fragments arranged by size: 

                 895     659     498
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     313     506     569     587     880    1013    1136 
   Size:        191     122     193      63      18     293     133     123

Cuts at:   1136    1196    1204    1352    1679    1967    2052 
   Size:         60       8     148     327     288      85

  Fragments arranged by size: 

                 327     293     288     193     191     148     133     123
                 122      85      63      60      18       8
 
BspHI T'CATG_A

Cuts at:      0     644    2052 
   Size:        644    1408
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     578    1520    2001    2052 
   Size:        578     942     481      51

  Fragments arranged by size: 

                 942     578     481      51
 
BsrBI CCG'CTC

Cuts at:      0      25     686    1791    2052 
   Size:         25     661    1105     261

  Fragments arranged by size: 

                1105     661     261      25
 
BsrDI GCAATG_nn'

Cuts at:      0    1365    2052 
   Size:       1365     687
 
BssSI C'ACGA_G

Cuts at:      0    1345    2052 
   Size:       1345     707
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     216    2052 
   Size:        216    1836
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     678    1272    2052 
   Size:        678     594     780

  Fragments arranged by size: 

                 780     678     594
 
BstYI r'GATC_y

Cuts at:      0     513    1691    2052 
   Size:        513    1178     361

  Fragments arranged by size: 

                1178     513     361
 
BtgI C'CryG_G

Cuts at:      0    1127    1247    2052 
   Size:       1127     120     805

  Fragments arranged by size: 

                1127     805     120
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1719    2052 
   Size:       1719     333
 
BtsI GCAGTG_nn'

Cuts at:      0     371     942    1646    2052 
   Size:        371     571     704     406

  Fragments arranged by size: 

                 704     571     406     371
 
ClaI AT'CG_AT

Cuts at:      0    2027    2052 
   Size:       2027      25
 
EaeI y'GGCC_r

Cuts at:      0     278     347     627     802     992    1442    2052 
   Size:        278      69     280     175     190     450     610

  Fragments arranged by size: 

                 610     450     280     278     190     175      69
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     323    1375    1443    2052 
   Size:         94     229    1052      68     609

  Fragments arranged by size: 

                1052     609     229      94      68
 
EcoICRI GAG'CTC

Cuts at:      0    1350    2052 
   Size:       1350     702
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     115     187     469     559     571     583     654 
   Size:         73      42      72     282      90      12      12      71

Cuts at:    654     721    1180    1294    1630    2052 
   Size:         67     459     114     336     422

  Fragments arranged by size: 

                 459     422     336     282     114      90      73      72
                  71      67      42      12      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     896    1042    2052 
   Size:        896     146    1010

  Fragments arranged by size: 

                1010     896     146
 
EcoO109I rG'GnC_Cy

Cuts at:      0     309     310     444    1551    1937    2052 
   Size:        309       1     134    1107     386     115

  Fragments arranged by size: 

                1107     386     309     134     115       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1044    2052 
   Size:       1044    1008
 
HaeII r_GCGC'y

Cuts at:      0    1273    1412    1568    1665    2052 
   Size:       1273     139     156      97     387

  Fragments arranged by size: 

                1273     387     156     139      97
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90     189     221    2052 
   Size:         58      32      99      32    1831

  Fragments arranged by size: 

                1831      99      58      32      32
 
KasI G'GCGC_C

Cuts at:      0    1408    2052 
   Size:       1408     644
 
MscI TGG'CCA

Cuts at:      0     280     349    2052 
   Size:        280      69    1703

  Fragments arranged by size: 

                1703     280      69
 
MslI CAynn'nnrTG

Cuts at:      0     184    1246    2052 
   Size:        184    1062     806

  Fragments arranged by size: 

                1062     806     184
 
MspA1I CmG'CkG

Cuts at:      0     213     611     678     987    1632    1712    2052 
   Size:        213     398      67     309     645      80     340

  Fragments arranged by size: 

                 645     398     340     309     213      80      67
 
NarI GG'CG_CC

Cuts at:      0    1409    2052 
   Size:       1409     643
 
NcoI C'CATG_G

Cuts at:      0    1247    2052 
   Size:       1247     805
 
NspI r_CATG'y

Cuts at:      0     915    1326    2052 
   Size:        915     411     726

  Fragments arranged by size: 

                 915     726     411
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     990    1635    2052 
   Size:        990     645     417

  Fragments arranged by size: 

                 990     645     417
 
PfoI T'CCnGG_A

Cuts at:      0     377     587    1394    1778    2052 
   Size:        377     210     807     384     274

  Fragments arranged by size: 

                 807     384     377     274     210
 
PpuMI rG'GwC_Cy

Cuts at:      0    1551    2052 
   Size:       1551     501
 
PshAI GACnn'nnGTC

Cuts at:      0    1918    2052 
   Size:       1918     134
 
PspOMI G'GGCC_C

Cuts at:      0     309    2052 
   Size:        309    1743
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     640     672    2052 
   Size:        640      32    1380

  Fragments arranged by size: 

                1380     640      32
 
PstI C_TGCA'G

Cuts at:      0     212     221    1379    1517    2052 
   Size:        212       9    1158     138     535

  Fragments arranged by size: 

                1158     535     212     138       9
 
PvuI CG_AT'CG

Cuts at:      0    2031    2052 
   Size:       2031      21
 
PvuII CAG'CTG

Cuts at:      0     678     987    1632    1712    2052 
   Size:        678     309     645      80     340

  Fragments arranged by size: 

                 678     645     340     309      80
 
SacI G_AGCT'C

Cuts at:      0    1352    2052 
   Size:       1352     700
 
SapI GCTCTTCn'nnn_

Cuts at:      0      94    1443    2052 
   Size:         94    1349     609

  Fragments arranged by size: 

                1349     609      94
 
SexAI A'CCwGG_T

Cuts at:      0    1187    2052 
   Size:       1187     865
 
SfcI C'TryA_G

Cuts at:      0     208     217    1375    1452    1513    1945    2052 
   Size:        208       9    1158      77      61     432     107

  Fragments arranged by size: 

                1158     432     208     107      77      61       9
 
SfoI GGC'GCC

Cuts at:      0    1410    2052 
   Size:       1410     642
 
SmlI C'TyrA_G

Cuts at:      0    1162    1600    2052 
   Size:       1162     438     452

  Fragments arranged by size: 

                1162     452     438
 
StuI AGG'CCT

Cuts at:      0    1861    2052 
   Size:       1861     191
 
StyI C'CwwG_G

Cuts at:      0    1247    1538    2052 
   Size:       1247     291     514

  Fragments arranged by size: 

                1247     514     291
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     943    2052 
   Size:        943    1109
 
TatI w'GTAC_w

Cuts at:      0    1316    1988    2052 
   Size:       1316     672      64

  Fragments arranged by size: 

                1316     672      64
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1108    1140    2052 
   Size:       1108      32     912

  Fragments arranged by size: 

                1108     912      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      72     240     256    2052 
   Size:         72     168      16    1796

  Fragments arranged by size: 

                1796     168      72      16
 
XbaI T'CTAG_A

Cuts at:      0     858    2052 
   Size:        858    1194
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     321    2052 
   Size:        321    1731
 
XmnI GAAnn'nnTTC

Cuts at:      0    1027    2052 
   Size:       1027    1025
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BclI
     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI    BpuEI     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BssSI   BstAPI    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     KasI     MscI
     MslI   MspA1I     NarI     NcoI     NspI    PflMI     PfoI    PpuMI
    PshAI   PspOMI     PsrI     PstI     PvuI    PvuII     SacI     SapI
    SexAI     SfcI     SfoI     SmlI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
    BciVI    BfrBI    BglII     BmrI     BmtI   Bpu10I    BsaAI    BsaBI
    BsaWI    BsiWI     BsmI    BspEI    BsrFI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI     PsiI    PspXI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     XhoI     XmaI     ZraI
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