Restriction map for pF1KSDA1058
 (Linear) (Six-base) MAPSORT of: KIAA1058.seq  Check: 6588  from: 1  to: 6204



KIAA1058.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AccI GT'mk_AC

Cuts at:      0     473    1250    2461    4301    6204 
   Size:        473     777    1211    1840    1903

  Fragments arranged by size: 

                1903    1840    1211     777     473
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1429    1540    2887    4031    5034    6204 
   Size:       1429     111    1347    1144    1003    1170

  Fragments arranged by size: 

                1429    1347    1170    1144    1003     111
 
AfeI AGC'GCT

Cuts at:      0    5185    6204 
   Size:       5185    1019
 
AflII C'TTAA_G

Cuts at:      0    2038    2554    4362    6043    6204 
   Size:       2038     516    1808    1681     161

  Fragments arranged by size: 

                2038    1808    1681     516     161
 
AflIII A'CryG_T

Cuts at:      0    2747    4415    6204 
   Size:       2747    1668    1789

  Fragments arranged by size: 

                2747    1789    1668
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2440    4920    6204 
   Size:       2440    2480    1284

  Fragments arranged by size: 

                2480    2440    1284
 
AlwNI CAG_nnn'CTG

Cuts at:      0     192     289    4092    4254    4635    6204 
   Size:        192      97    3803     162     381    1569

  Fragments arranged by size: 

                3803    1569     381     192     162      97
 
ApaI G_GGCC'C

Cuts at:      0     118    6204 
   Size:        118    6086
 
ApoI r'AATT_y

Cuts at:      0     639    1867    2002    2122    2432    3196    3855    5477 
   Size:        639    1228     135     120     310     764     659    1622

Cuts at:   5477    5512    6204 
   Size:         35     692

  Fragments arranged by size: 

                1622    1228     764     692     659     639     310     135
                 120      35
 
AvaI C'yCGr_G

Cuts at:      0     111    2672    4828    5316    6124    6204 
   Size:        111    2561    2156     488     808      80

  Fragments arranged by size: 

                2561    2156     808     488     111      80
 
BamHI G'GATC_C

Cuts at:      0     625    1097    4865    6204 
   Size:        625     472    3768    1339

  Fragments arranged by size: 

                3768    1339     625     472
 
BanI G'GyrC_C

Cuts at:      0    3679    6204 
   Size:       3679    2525
 
BanII G_rGCy'C

Cuts at:      0     112     118     749    1327    2523    4471    4835    5147 
   Size:        112       6     631     578    1196    1948     364     312

Cuts at:   5147    5195    5882    6204 
   Size:         48     687     322

  Fragments arranged by size: 

                1948    1196     687     631     578     364     322     312
                 112      48       6
 
BbeI G_GCGC'C

Cuts at:      0    3683    6204 
   Size:       3683    2521
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     187    2030    3072    3114    4344    4846    5102    6122 
   Size:        187    1843    1042      42    1230     502     256    1020

Cuts at:   6122    6204 
   Size:         82

  Fragments arranged by size: 

                1843    1230    1042    1020     502     256     187      82
                  42
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     257     291    5179    5213    6204 
   Size:        257      34    4888      34     991

  Fragments arranged by size: 

                4888     991     257      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1379    4715    6204 
   Size:       1379    3336    1489

  Fragments arranged by size: 

                3336    1489    1379
 
BclI T'GATC_A

Cuts at:      0    2897    5915    6204 
   Size:       2897    3018     289

  Fragments arranged by size: 

                3018    2897     289
 
BfrBI ATG'CAT

Cuts at:      0    1603    4074    6189    6204 
   Size:       1603    2471    2115      15

  Fragments arranged by size: 

                2471    2115    1603      15
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1534    4930    5021    6204 
   Size:       1534    3396      91    1183

  Fragments arranged by size: 

                3396    1534    1183      91
 
BglII A'GATC_T

Cuts at:      0     659    6204 
   Size:        659    5545
 
BlpI GC'TnA_GC

Cuts at:      0    1055    1469    1724    2759    2912    4465    6204 
   Size:       1055     414     255    1035     153    1553    1739

  Fragments arranged by size: 

                1739    1553    1055    1035     414     255     153
 
Bme1580I G_kGCm'C

Cuts at:      0     118    1877    6204 
   Size:        118    1759    4327

  Fragments arranged by size: 

                4327    1759     118
 
BmgBI CAC'GTC

Cuts at:      0    1292    4844    5100    5607    6204 
   Size:       1292    3552     256     507     597

  Fragments arranged by size: 

                3552    1292     597     507     256
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1726    2691    6204 
   Size:       1726     965    3513

  Fragments arranged by size: 

                3513    1726     965
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2043    3776    3778    5100    6204 
   Size:       2043    1733       2    1322    1104

  Fragments arranged by size: 

                2043    1733    1322    1104       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    2537    3182    3274    3358    4777    5920    6204 
   Size:        820    1717     645      92      84    1419    1143     284

  Fragments arranged by size: 

                1717    1419    1143     820     645     284      92      84
            
 
Bpu10I CC'TnA_GC

Cuts at:      0      86    4834    6087    6204 
   Size:         86    4748    1253     117

  Fragments arranged by size: 

                4748    1253     117      86
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     733    1321    1669    2199    2748    2812    3400    4429 
   Size:        733     588     348     530     549      64     588    1029

Cuts at:   4429    5170    6204 
   Size:        741    1034

  Fragments arranged by size: 

                1034    1029     741     733     588     588     549     530
                 348      64
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2331    3158    6204 
   Size:       2331     827    3046

  Fragments arranged by size: 

                3046    2331     827
 
BsaAI yAC'GTr

Cuts at:      0    1248    2583    4917    6204 
   Size:       1248    1335    2334    1287

  Fragments arranged by size: 

                2334    1335    1287    1248
 
BsaBI GATnn'nnATC

Cuts at:      0    3403    6204 
   Size:       3403    2801
 
BsaHI Gr'CG_yC

Cuts at:      0    3628    3680    6204 
   Size:       3628      52    2524

  Fragments arranged by size: 

                3628    2524      52
 
BsaWI w'CCGG_w

Cuts at:      0     925    1223    2391    4550    6204 
   Size:        925     298    1168    2159    1654

  Fragments arranged by size: 

                2159    1654    1168     925     298
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3702    3732    6204 
   Size:       3702      30    2472

  Fragments arranged by size: 

                3702    2472      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     481    4683    5022    5025    5092    5886    6204 
   Size:        481    4202     339       3      67     794     318

  Fragments arranged by size: 

                4202     794     481     339     318      67       3
 
BseYI C'CCAG_C

Cuts at:      0    3675    4092    4490    6198    6204 
   Size:       3675     417     398    1708       6

  Fragments arranged by size: 

                3675    1708     417     398       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3646    4006    4141    5263    6204 
   Size:       3646     360     135    1122     941

  Fragments arranged by size: 

                3646    1122     941     360     135
 
BsiEI CG_ry'CG

Cuts at:      0    5903    6204 
   Size:       5903     301
 
BsiHKAI G_wGCw'C

Cuts at:      0     749    3416    4471    4835    5147    5195    5882    5896 
   Size:        749    2667    1055     364     312      48     687      14

Cuts at:   5896    6204 
   Size:        308

  Fragments arranged by size: 

                2667    1055     749     687     364     312     308      48
                  14
 
BsmI GAATG_Cn'

Cuts at:      0    1530    1568    3435    6204 
   Size:       1530      38    1867    2769

  Fragments arranged by size: 

                2769    1867    1530      38
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     249     585    6204 
   Size:        249     336    5619

  Fragments arranged by size: 

                5619     336     249
 
Bsp1286I G_dGCh'C

Cuts at:      0     112     118     749    1327    1877    2523    3416    4471 
   Size:        112       6     631     578     550     646     893    1055

Cuts at:   4471    4835    5147    5195    5882    5896    6204 
   Size:        364     312      48     687      14     308

  Fragments arranged by size: 

                1055     893     687     646     631     578     550     364
                 312     308     112      48      14       6
 
BspEI T'CCGG_A

Cuts at:      0    2391    4550    6204 
   Size:       2391    2159    1654

  Fragments arranged by size: 

                2391    2159    1654
 
BspHI T'CATG_A

Cuts at:      0    1350    4280    6204 
   Size:       1350    2930    1924

  Fragments arranged by size: 

                2930    1924    1350
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     950    1840    3431    3503    3680    6204 
   Size:        950     890    1591      72     177    2524

  Fragments arranged by size: 

                2524    1591     950     890     177      72
 
BsrBI CCG'CTC

Cuts at:      0    4411    6204 
   Size:       4411    1793
 
BsrDI GCAATG_nn'

Cuts at:      0     863    1119    1141    1461    3692    4181    4907    5195 
   Size:        863     256      22     320    2231     489     726     288

Cuts at:   5195    6204 
   Size:       1009

  Fragments arranged by size: 

                2231    1009     863     726     489     320     288     256
                  22
 
BsrFI r'CCGG_y

Cuts at:      0     123    4667    6204 
   Size:        123    4544    1537

  Fragments arranged by size: 

                4544    1537     123
 
BsrGI T'GTAC_A

Cuts at:      0     539    6204 
   Size:        539    5665
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     514    1574    2368    2445    4132    5844    6204 
   Size:        514    1060     794      77    1687    1712     360

  Fragments arranged by size: 

                1712    1687    1060     794     514     360      77
 
BstYI r'GATC_y

Cuts at:      0     188     625     659     830    1097    3757    4865    5568 
   Size:        188     437      34     171     267    2660    1108     703

Cuts at:   5568    5662    6204 
   Size:         94     542

  Fragments arranged by size: 

                2660    1108     703     542     437     267     188     171
                  94      34
 
BstZ17I GTA'TAC

Cuts at:      0    2462    6204 
   Size:       2462    3742
 
Bsu36I CC'TnA_GG

Cuts at:      0    2529    5005    5564    6204 
   Size:       2529    2476     559     640

  Fragments arranged by size: 

                2529    2476     640     559
 
BtgI C'CryG_G

Cuts at:      0    3860    4263    6204 
   Size:       3860     403    1941

  Fragments arranged by size: 

                3860    1941     403
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     635    2584    6204 
   Size:        635    1949    3620

  Fragments arranged by size: 

                3620    1949     635
 
BtsI GCAGTG_nn'

Cuts at:      0    1320    2422    2990    4355    5161    5746    5772    6204 
   Size:       1320    1102     568    1365     806     585      26     432

  Fragments arranged by size: 

                1365    1320    1102     806     585     568     432      26
            
 
DraI TTT'AAA

Cuts at:      0    4343    6204 
   Size:       4343    1861
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     263     469     579    6204 
   Size:        263     206     110    5625

  Fragments arranged by size: 

                5625     263     206     110
 
EaeI y'GGCC_r

Cuts at:      0     245    1424    4134    4256    4739    4805    4859    5846 
   Size:        245    1179    2710     122     483      66      54     987

Cuts at:   5846    5900    6204 
   Size:         54     304

  Fragments arranged by size: 

                2710    1179     987     483     304     245     122      66
                  54      54
 
EagI C'GGCC_G

Cuts at:      0    5900    6204 
   Size:       5900     304
 
EarI CTCTTCn'nnn_

Cuts at:      0     226     296    1073    1500    2230    3805    5710    5729 
   Size:        226      70     777     427     730    1575    1905      19

Cuts at:   5729    6204 
   Size:        475

  Fragments arranged by size: 

                1905    1575     777     730     475     427     226      70
                  19
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     313    5665    5890    6204 
   Size:        313    5352     225     314

  Fragments arranged by size: 

                5352     314     313     225
 
EcoICRI GAG'CTC

Cuts at:      0     747    4469    4833    5145    5193    5880    6204 
   Size:        747    3722     364     312      48     687     324

  Fragments arranged by size: 

                3722     747     687     364     324     312      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1429    1540    2537    2887    3182    3274    3358 
   Size:        820     609     111     997     350     295      92      84

Cuts at:   3358    4031    4777    5034    5920    6204 
   Size:        673     746     257     886     284

  Fragments arranged by size: 

                 997     886     820     746     673     609     350     295
                 284     257     111      92      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3664    5004    6204 
   Size:       3664    1340    1200

  Fragments arranged by size: 

                3664    1340    1200
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115    1162    3669    4367    5336    6204 
   Size:        115    1047    2507     698     969     868

  Fragments arranged by size: 

                2507    1047     969     868     698     115
 
EcoRI G'AATT_C

Cuts at:      0    2002    6204 
   Size:       2002    4202
 
EcoRV GAT'ATC

Cuts at:      0    6019    6204 
   Size:       6019     185
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1059    3195    4560    6204 
   Size:       1059    2136    1365    1644

  Fragments arranged by size: 

                2136    1644    1365    1059
 
FspI TGC'GCA

Cuts at:      0    5160    6204 
   Size:       5160    1044
 
HaeII r_GCGC'y

Cuts at:      0    1303    2087    3683    5000    5187    6204 
   Size:       1303     784    1596    1317     187    1017

  Fragments arranged by size: 

                1596    1317    1303    1017     784     187
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     478     510    1033    1065    6204 
   Size:        478      32     523      32    5139

  Fragments arranged by size: 

                5139     523     478      32      32
 
HincII GTy'rAC

Cuts at:      0     462     474    2664    5559    6204 
   Size:        462      12    2190    2895     645

  Fragments arranged by size: 

                2895    2190     645     462      12
 
HindIII A'AGCT_T

Cuts at:      0    1036    6071    6204 
   Size:       1036    5035     133

  Fragments arranged by size: 

                5035    1036     133
 
HpaI GTT'AAC

Cuts at:      0    2664    6204 
   Size:       2664    3540
 
KasI G'GCGC_C

Cuts at:      0    3679    6204 
   Size:       3679    2525
 
MfeI C'AATT_G

Cuts at:      0    1760    6204 
   Size:       1760    4444
 
MluI A'CGCG_T

Cuts at:      0    2747    6204 
   Size:       2747    3457
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     410    5453    6204 
   Size:        410    5043     751

  Fragments arranged by size: 

                5043     751     410
 
MscI TGG'CCA

Cuts at:      0    1426    4136    4741    4807    4861    5848    6204 
   Size:       1426    2710     605      66      54     987     356

  Fragments arranged by size: 

                2710    1426     987     605     356      66      54
 
MslI CAynn'nnrTG

Cuts at:      0     452     568     890    3103    3409    3580    4130    5267 
   Size:        452     116     322    2213     306     171     550    1137

Cuts at:   5267    5596    5842    6204 
   Size:        329     246     362

  Fragments arranged by size: 

                2213    1137     550     452     362     329     322     306
                 246     171     116
 
MspA1I CmG'CkG

Cuts at:      0    1054    2160    2204    2619    3531    3610    3621    4092 
   Size:       1054    1106      44     415     912      79      11     471

Cuts at:   4092    4494    4587    5469    5889    6198    6204 
   Size:        402      93     882     420     309       6

  Fragments arranged by size: 

                1106    1054     912     882     471     420     415     402
                 309      93      79      44      11       6
 
NaeI GCC'GGC

Cuts at:      0     125    6204 
   Size:        125    6079
 
NarI GG'CG_CC

Cuts at:      0    3680    6204 
   Size:       3680    2524
 
NcoI C'CATG_G

Cuts at:      0    4263    6204 
   Size:       4263    1941
 
NdeI CA'TA_TG

Cuts at:      0     274    2736    2766    5979    6204 
   Size:        274    2462      30    3213     225

  Fragments arranged by size: 

                3213    2462     274     225      30
 
NgoMIV G'CCGG_C

Cuts at:      0     123    6204 
   Size:        123    6081
 
NsiI A_TGCA'T

Cuts at:      0    1605    4076    6191    6204 
   Size:       1605    2471    2115      13

  Fragments arranged by size: 

                2471    2115    1605      13
 
NspI r_CATG'y

Cuts at:      0    1541    1683    2569    3939    4078    4419    6204 
   Size:       1541     142     886    1370     139     341    1785

  Fragments arranged by size: 

                1785    1541    1370     886     341     142     139
 
PciI A'CATG_T

Cuts at:      0    4415    6204 
   Size:       4415    1789
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3336    4038    4254    6204 
   Size:       3336     702     216    1950

  Fragments arranged by size: 

                3336    1950     702     216
 
PfoI T'CCnGG_A

Cuts at:      0    3159    3374    3383    6204 
   Size:       3159     215       9    2821

  Fragments arranged by size: 

                3159    2821     215       9
 
PmlI CAC'GTG

Cuts at:      0    2583    6204 
   Size:       2583    3621
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1249    1281    5444    5476    6204 
   Size:       1249      32    4163      32     728

  Fragments arranged by size: 

                4163    1249     728      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1162    3669    4367    5336    6204 
   Size:       1162    2507     698     969     868

  Fragments arranged by size: 

                2507    1162     969     868     698
 
PshAI GACnn'nnGTC

Cuts at:      0    2674    5305    5605    6204 
   Size:       2674    2631     300     599

  Fragments arranged by size: 

                2674    2631     599     300
 
PsiI TTA'TAA

Cuts at:      0     647    4379    6204 
   Size:        647    3732    1825

  Fragments arranged by size: 

                3732    1825     647
 
PspOMI G'GGCC_C

Cuts at:      0     114    6204 
   Size:        114    6090
 
PspXI vC'TCGA_Gb

Cuts at:      0    6124    6204 
   Size:       6124      80
 
PstI C_TGCA'G

Cuts at:      0    1837    2097    3325    5927    6204 
   Size:       1837     260    1228    2602     277

  Fragments arranged by size: 

                2602    1837    1228     277     260
 
PvuII CAG'CTG

Cuts at:      0    1054    2160    2204    2619    3610    4092    4494    4587 
   Size:       1054    1106      44     415     991     482     402      93

Cuts at:   4587    5889    6198    6204 
   Size:       1302     309       6

  Fragments arranged by size: 

                1302    1106    1054     991     482     415     402     309
                  93      44       6
 
SacI G_AGCT'C

Cuts at:      0     749    4471    4835    5147    5195    5882    6204 
   Size:        749    3722     364     312      48     687     322

  Fragments arranged by size: 

                3722     749     687     364     322     312      48
 
SalI G'TCGA_C

Cuts at:      0     472    6204 
   Size:        472    5732
 
SapI GCTCTTCn'nnn_

Cuts at:      0     226    3805    5729    6204 
   Size:        226    3579    1924     475

  Fragments arranged by size: 

                3579    1924     475     226
 
ScaI AGT'ACT

Cuts at:      0    1942    2497    2872    6204 
   Size:       1942     555     375    3332

  Fragments arranged by size: 

                3332    1942     555     375
 
SexAI A'CCwGG_T

Cuts at:      0    2058    6204 
   Size:       2058    4146
 
SfcI C'TryA_G

Cuts at:      0    1644    1833    2093    2453    3321    5289    5923    6153 
   Size:       1644     189     260     360     868    1968     634     230

Cuts at:   6153    6204 
   Size:         51

  Fragments arranged by size: 

                1968    1644     868     634     360     260     230     189
                  51
 
SfoI GGC'GCC

Cuts at:      0    3681    6204 
   Size:       3681    2523
 
SmaI CCC'GGG

Cuts at:      0     113    6204 
   Size:        113    6091
 
SmlI C'TyrA_G

Cuts at:      0     748    1336    1684    2038    2214    2554    2727    2827 
   Size:        748     588     348     354     176     340     173     100

Cuts at:   2827    3415    4362    4444    5149    6043    6124    6204 
   Size:        588     947      82     705     894      81      80

  Fragments arranged by size: 

                 947     894     748     705     588     588     354     348
                 340     176     173     100      82      81      80
 
SphI G_CATG'C

Cuts at:      0    1541    2569    4078    6204 
   Size:       1541    1028    1509    2126

  Fragments arranged by size: 

                2126    1541    1509    1028
 
SrfI GCCC'GGGC

Cuts at:      0     113    6204 
   Size:        113    6091
 
SspI AAT'ATT

Cuts at:      0     686    1385    1986    6204 
   Size:        686     699     601    4218

  Fragments arranged by size: 

                4218     699     686     601
 
StuI AGG'CCT

Cuts at:      0    4486    5083    6204 
   Size:       4486     597    1121

  Fragments arranged by size: 

                4486    1121     597
 
StyI C'CwwG_G

Cuts at:      0     498    1973    4263    6204 
   Size:        498    1475    2290    1941

  Fragments arranged by size: 

                2290    1941    1475     498
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     694    5317    6204 
   Size:        694    4623     887

  Fragments arranged by size: 

                4623     887     694
 
TatI w'GTAC_w

Cuts at:      0     539    1940    2495    2870    2892    4034    4796    5501 
   Size:        539    1401     555     375      22    1142     762     705

Cuts at:   5501    6204 
   Size:        703

  Fragments arranged by size: 

                1401    1142     762     705     703     555     539     375
                  22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2285    2317    3845    3877    5725    5757    6204 
   Size:       2285      32    1528      32    1848      32     447

  Fragments arranged by size: 

                2285    1848    1528     447      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     263    6204 
   Size:        263    5941
 
XbaI T'CTAG_A

Cuts at:      0    1749    6204 
   Size:       1749    4455
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4876    6204 
   Size:       4876    1328
 
XhoI C'TCGA_G

Cuts at:      0    6124    6204 
   Size:       6124      80
 
XmaI C'CCGG_G

Cuts at:      0     111    6204 
   Size:        111    6093
 
XmnI GAAnn'nnTTC

Cuts at:      0    1261    1386    6204 
   Size:       1261     125    4818

  Fragments arranged by size: 

                4818    1261     125
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII   AflIII     AlfI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NsiI     NspI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI    PvuII     SacI     SalI
     SapI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     SphI
     SrfI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AgeI     AhdI     AleI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BmtI
    BsiWI   BssHII    BssSI   BstAPI    BstBI   BstEII     ClaI    CspCI
   DraIII     FseI    FspAI     KpnI     NheI     NotI     NruI     PacI
     PasI     PmeI     PsrI     PvuI    RsrII    SacII    SanDI     SbfI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SwaI     ZraI
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