Restriction map for pF1KSDA1052
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        AvrII       BclI        BfrI        BlnI        
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        KspI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII         527  GACGTC               GACGT^C
Acc65I       1409  GGTACC               G^GTACC
AccII         149  CGCG                 CG^CG
Asp700I      3087  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1409  GGTACC               G^GTACC
AspI          920  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        2479  CACGTG               CAC^GTG
BcgI         2214  CGAGCAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         462  GGATAC               GTATCC(6/5)
BfaI           71  CTAG                 C^TAG
BglI         2827  GCCAGATTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3186  AGATCT               A^GATCT
BsaAI        2479  CACGTG               [CT]AC^GT[GA]
BspLU11I     4136  ACATGT               A^CATGT
BstUI         149  CGCG                 CG^CG
HincII        309  GTCAAC               GT[CT]^[GA]AC
HindII        309  GTCAAC               GT[CT]^[GA]AC
KpnI         1409  GGTACC               GGTAC^C
MaeI           71  CTAG                 C^TAG
MseI          326  TTAA                 T^TAA
MvnI          149  CGCG                 CG^CG
NaeI         2753  GCCGGC               GCC^GGC
NgoMIV       2753  GCCGGC               G^CCGGC
NspI         4136  ACATGT               [GA]CATG^[CT]
PaeR7I       1816  CTCGAG               C^TCGAG
PflFI         920  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2479  CACGTG               CAC^GTG
PmlI         2479  CACGTG               CAC^GTG
PshAI         868  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         1812  AGTACT               AGT^ACT
SexAI         921  ACCTGGT              A^CC[AT]GGT
Tru9I         326  TTAA                 T^TAA
Tth111I       920  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1816  CTCGAG               C^TCGAG
XmnI         3087  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI         1410  GTAC                 GT^AC
             1813  GTAC                
AflIII       4136  ACATGT               A^C[GA][CT]GT
             4248  ACGTGT              
AviII        3441  TGCGCA               TGC^GCA
             3491  TGCGCA              
BamHI        1680  GGATCC               G^GATCC
             3643  GGATCC              
BbeI         2750  GGCGCC               GGCGC^C
             3422  GGCGCC              
BsaWI        2662  ACCGGA               [AT]^CCGG[AT]
             4232  ACCGGA              
BsmI         2229  GAATGC               GAATGC(1/-1)
             1635  GCATTC              
BsrFI         826  GCCGGT               [GA]^CCGG[CT]
             2753  GCCGGC              
BssSI        1438  CACGAG               CACGAG(-5/-1)
             2497  CACGAG              
BstAPI       1863  GCAGCAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2877  GCAGCAGCTGC         
BstEII        981  GGTGACC              G^GT[ACGT]ACC
             4268  GGTTACC             
BstPI         981  GGTGACC              G^GT[ACGT]ACC
             4268  GGTTACC             
Cfr10I        826  GCCGGT               [GA]^CCGG[CT]
             2753  GCCGGC              
EcoNI         445  CCTTTACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3501  CCTGGAGAAGG         
EcoO65I       981  GGTGACC              G^GT[ACGT]ACC
             4268  GGTTACC             
FspI         3441  TGCGCA               TGC^GCA
             3491  TGCGCA              
HgaI         1141  GACGC                GACGC(5/10)
             2733  GCGTC               
KasI         2750  GGCGCC               G^GCGCC
             3422  GGCGCC              
MslI          256  CATCCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3520  CACCTGGATG          
NarI         2750  GGCGCC               GG^CGCC
             3422  GGCGCC              
NcoI         1224  CCATGG               C^CATGG
             1790  CCATGG              
PpuMI        3200  AGGACCT              [GA]G^G[AT]CC[CT]
             3896  GGGACCC             
RsaI         1410  GTAC                 GT^AC
             1813  GTAC                
SfoI         2750  GGCGCC               GGC^GCC
             3422  GGCGCC              
SmaI           98  CCCGGG               CCC^GGG
             3893  CCCGGG              
StuI         1395  AGGCCT               AGG^CCT
             3617  AGGCCT              
XmaCI          98  CCCGGG               C^CCGGG
             3893  CCCGGG              
XmaI           98  CCCGGG               C^CCGGG
             3893  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AciI        AcsI        AcyI        AhdI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
AspEI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eam1105I    EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PstI        
PvuII       SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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