Restriction map for pF1KSDA1034
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AvaI        AviII       AvrII       BcgI        BciVI       
BclI        BfrI        BlnI        BsaWI       BseAI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       FbaI        FseI        FspI        HincII      
HindII      HpaI        MfeI        MluI        MroI        
MslI        MunI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SalI        SapI        
SbfI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspI        SwaI        XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII          64  GACGTC               GACGT^C
Acc65I       2000  GGTACC               G^GTACC
AflIII       1707  ACACGT               A^C[GA][CT]GT
Asp718I      2000  GGTACC               G^GTACC
AspI         2085  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        1713  GGTCC                G^G[AT]CC
BalI          152  TGGCCA               TGG^CCA
BamHI        1880  GGATCC               G^GATCC
BbvCI        2143  GCTGAGG              CCTCAGC(-5/-2)
BfaI         1234  CTAG                 C^TAG
BglI         1516  GCCAAAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1859  AGATCT               A^GATCT
BlpI         1948  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1948  GCTCAGC              GC^T[ACGT]AGC
BsaBI         433  GATGTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1735  GTGCAG               GTGCAG(16/14)
BsiEI          42  CGACCG               CG[GA][CT]^CG
Bsp1407I     2129  TGTACA               T^GTACA
BsrDI         527  GCAATG               GCAATG(2/0)
BsrGI        2129  TGTACA               T^GTACA
BstAPI       2173  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1948  GCTCAGC              GC^T[ACGT]AGC
DraII          72  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I       72  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1087  GATATC               GAT^ATC
EcoT22I       757  ATGCAT               ATGCA^T
HindIII      1890  AAGCTT               A^AGCTT
KpnI         2000  GGTACC               GGTAC^C
MaeI         1234  CTAG                 C^TAG
MamI          433  GATGTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         152  TGGCCA               TGG^CCA
MscI          152  TGGCCA               TGG^CCA
NaeI          971  GCCGGC               GCC^GGC
NgoMIV        971  GCCGGC               G^CCGGC
NsiI          757  ATGCAT               ATGCA^T
PflFI        2085  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         282  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1751  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI          835  AGTACT               AGT^ACT
SphI         1700  GCATGC               GCATG^C
SspBI        2129  TGTACA               T^GTACA
StuI         1908  AGGCCT               AGG^CCT
TaqI         1396  TCGA                 T^CGA
Tth111I      2085  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1396  TCGA                 T^CGA
Van91I        282  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          824  AAATTC               [GA]^AATT[CT]
             1257  GAATTT              
AlwNI        1012  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2174  CAGCACCTG           
AocI         1242  CCTGAGG              CC^T[ACGT]AGG
             1381  CCTCAGG             
ApoI          824  AAATTC               [GA]^AATT[CT]
             1257  GAATTT              
AspHI         564  GAGCAC               G[AT]GC[AT]^C
             2032  GAGCAC              
BbeI          145  GGCGCC               GGCGC^C
             1432  GGCGCC              
BbrPI        1708  CACGTG               CAC^GTG
             2005  CACGTG              
BsaAI        1708  CACGTG               [CT]AC^GT[GA]
             2005  CACGTG              
BsaI         1341  GGTCTC               GGTCTC(1/5)
             1847  GGTCTC              
BsiHKAI       564  GAGCAC               G[AT]GC[AT]^C
             2032  GAGCAC              
BsmI          580  GAATGC               GAATGC(1/-1)
             1156  GCATTC              
BspHI         875  TCATGA               T^CATGA
             1899  TCATGA              
BspMI        2178  ACCTGC               ACCTGC(4/8)
             1737  GCAGGT              
BsrI           82  CCAGT                ACTGG(1/-1)
             1124  CCAGT               
BstXI         155  CCAAGGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              657  CCAGGAGTTTGG        
Bsu36I       1242  CCTGAGG              CC^T[ACGT]AGG
             1381  CCTCAGG             
EaeI          152  TGGCCA               [CT]^GGCC[GA]
              416  TGGCCG              
Eco81I       1242  CCTGAGG              CC^T[ACGT]AGG
             1381  CCTCAGG             
HgaI          406  GACGC                GACGC(5/10)
              750  GCGTC               
KasI          145  GGCGCC               G^GCGCC
             1432  GGCGCC              
KspI          129  CCGCGG               CCGC^GG
             2045  CCGCGG              
MaeIII        391  GTGAC                ^GT[ACGT]AC
              448  GTGAC               
NarI          145  GGCGCC               GG^CGCC
             1432  GGCGCC              
NcoI          119  CCATGG               C^CATGG
              935  CCATGG              
NspI          422  ACATGC               [GA]CATG^[CT]
             1700  GCATGC              
PleI         1675  GAGTC                GAGTC(4/5)
             2089  GAGTC               
PmaCI        1708  CACGTG               CAC^GTG
             2005  CACGTG              
PmlI         1708  CACGTG               CAC^GTG
             2005  CACGTG              
RcaI          875  TCATGA               T^CATGA
             1899  TCATGA              
SacII         129  CCGCGG               CCGC^GG
             2045  CCGCGG              
SfoI          145  GGCGCC               GGC^GCC
             1432  GGCGCC              
SmlI          995  CTCAAG               C^T[CT][GA]AG
             1502  CTCAAG              
Tsp45I        391  GTGAC                ^GT[GC]AC
              448  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        BanI        BanII       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrBI       BsrFI       BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XcmI        XhoII       
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