Restriction map for pF1KSDA1034
 (Linear) (Six-base) MAPSORT of: KIAA1034.seq  Check: 1740  from: 1  to: 2199



KIAA1034.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2187    2199 
   Size:       2187      12
 
AatII G_ACGT'C

Cuts at:      0      68    2199 
   Size:         68    2131
 
Acc65I G'GTAC_C

Cuts at:      0    2000    2199 
   Size:       2000     199
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1135    1841    2199 
   Size:       1135     706     358

  Fragments arranged by size: 

                1135     706     358
 
AflIII A'CryG_T

Cuts at:      0    1707    2199 
   Size:       1707     492
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     669    2199 
   Size:        669    1530
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1017    2179    2199 
   Size:       1017    1162      20

  Fragments arranged by size: 

                1162    1017      20
 
ApoI r'AATT_y

Cuts at:      0     824    1257    2199 
   Size:        824     433     942

  Fragments arranged by size: 

                 942     824     433
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1599    1632    2199 
   Size:       1599      33     567

  Fragments arranged by size: 

                1599     567      33
 
BamHI G'GATC_C

Cuts at:      0    1880    2199 
   Size:       1880     319
 
BanI G'GyrC_C

Cuts at:      0     145    1432    2000    2199 
   Size:        145    1287     568     199

  Fragments arranged by size: 

                1287     568     199     145
 
BanII G_rGCy'C

Cuts at:      0      22     129     883    2044    2199 
   Size:         22     107     754    1161     155

  Fragments arranged by size: 

                1161     754     155     107      22
 
BbeI G_GCGC'C

Cuts at:      0     149    1436    2199 
   Size:        149    1287     763

  Fragments arranged by size: 

                1287     763     149
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     494     880    1211    1829    2199 
   Size:        494     386     331     618     370

  Fragments arranged by size: 

                 618     494     386     370     331
 
BbvCI CC'TCA_GC

Cuts at:      0    2144    2199 
   Size:       2144      55
 
BfrBI ATG'CAT

Cuts at:      0     759    2199 
   Size:        759    1440
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1522    2199 
   Size:       1522     677
 
BglII A'GATC_T

Cuts at:      0    1859    2199 
   Size:       1859     340
 
BlpI GC'TnA_GC

Cuts at:      0    1949    2199 
   Size:       1949     250
 
Bme1580I G_kGCm'C

Cuts at:      0     860    2199 
   Size:        860    1339
 
BmgBI CAC'GTC

Cuts at:      0    2055    2199 
   Size:       2055     144
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      76    2199 
   Size:         76    2123
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1799    2133    2199 
   Size:       1799     334      66

  Fragments arranged by size: 

                1799     334      66
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1067    1338    2199 
   Size:        121     946     271     861

  Fragments arranged by size: 

                 946     861     271     121
 
Bpu10I CC'TnA_GC

Cuts at:      0    1727    2144    2199 
   Size:       1727     417      55

  Fragments arranged by size: 

                1727     417      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     980    1487    2199 
   Size:        980     507     712

  Fragments arranged by size: 

                 980     712     507
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1347    1853    2199 
   Size:       1347     506     346

  Fragments arranged by size: 

                1347     506     346
 
BsaAI yAC'GTr

Cuts at:      0    1710    2007    2199 
   Size:       1710     297     192

  Fragments arranged by size: 

                1710     297     192
 
BsaBI GATnn'nnATC

Cuts at:      0     437    2199 
   Size:        437    1762
 
BsaHI Gr'CG_yC

Cuts at:      0      65     146    1433    2199 
   Size:         65      81    1287     766

  Fragments arranged by size: 

                1287     766      81      65
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2026    2056    2199 
   Size:       2026      30     143

  Fragments arranged by size: 

                2026     143      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     803    1335    1359    1684    1687    1802    2089    2110 
   Size:        803     532      24     325       3     115     287      21

Cuts at:   2110    2137    2161    2199 
   Size:         27      24      38

  Fragments arranged by size: 

                 803     532     325     287     115      38      27      24
                  24      21       3
 
BseYI C'CCAG_C

Cuts at:      0     357     899    1363    1643    2199 
   Size:        357     542     464     280     556

  Fragments arranged by size: 

                 556     542     464     357     280
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1756    2199 
   Size:       1756     443
 
BsiEI CG_ry'CG

Cuts at:      0      45    2199 
   Size:         45    2154
 
BsiHKAI G_wGCw'C

Cuts at:      0     568    2036    2199 
   Size:        568    1468     163

  Fragments arranged by size: 

                1468     568     163
 
BsmI GAATG_Cn'

Cuts at:      0     586    1156    2199 
   Size:        586     570    1043

  Fragments arranged by size: 

                1043     586     570
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     129     568     860     883    2036    2044    2199 
   Size:         22     107     439     292      23    1153       8     155

  Fragments arranged by size: 

                1153     439     292     155     107      23      22       8
            
 
BspHI T'CATG_A

Cuts at:      0     875    1899    2199 
   Size:        875    1024     300

  Fragments arranged by size: 

                1024     875     300
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1728    2187    2199 
   Size:       1728     459      12

  Fragments arranged by size: 

                1728     459      12
 
BsrBI CCG'CTC

Cuts at:      0       6      53    1314    2199 
   Size:          6      47    1261     885

  Fragments arranged by size: 

                1261     885      47       6
 
BsrDI GCAATG_nn'

Cuts at:      0     534    2199 
   Size:        534    1665
 
BsrFI r'CCGG_y

Cuts at:      0      44     971    1731    2199 
   Size:         44     927     760     468

  Fragments arranged by size: 

                 927     760     468      44
 
BsrGI T'GTAC_A

Cuts at:      0    2129    2199 
   Size:       2129      70
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2179    2199 
   Size:       2179      20
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     162     664    2199 
   Size:        162     502    1535

  Fragments arranged by size: 

                1535     502     162
 
BstYI r'GATC_y

Cuts at:      0    1469    1859    1880    1956    2199 
   Size:       1469     390      21      76     243

  Fragments arranged by size: 

                1469     390     243      76      21
 
Bsu36I CC'TnA_GG

Cuts at:      0    1243    1382    2199 
   Size:       1243     139     817

  Fragments arranged by size: 

                1243     817     139
 
BtgI C'CryG_G

Cuts at:      0     119     129     149     161     935    1070    2016    2045 
   Size:        119      10      20      12     774     135     946      29

Cuts at:   2045    2199 
   Size:        154

  Fragments arranged by size: 

                 946     774     154     135     119      29      20      12
                  10
 
BtsI GCAGTG_nn'

Cuts at:      0     511    2199 
   Size:        511    1688
 
EaeI y'GGCC_r

Cuts at:      0     152     416    2199 
   Size:        152     264    1783

  Fragments arranged by size: 

                1783     264     152
 
EarI CTCTTCn'nnn_

Cuts at:      0    1071    1111    1235    2199 
   Size:       1071      40     124     964

  Fragments arranged by size: 

                1071     964     124      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      24      63    2199 
   Size:         24      39    2136

  Fragments arranged by size: 

                2136      39      24
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1067    1135    1338    1841    2199 
   Size:        121     946      68     203     503     358

  Fragments arranged by size: 

                 946     503     358     203     121      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0      73    2199 
   Size:         73    2126
 
EcoRV GAT'ATC

Cuts at:      0    1089    2199 
   Size:       1089    1110
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     289    1881    2199 
   Size:        289    1592     318

  Fragments arranged by size: 

                1592     318     289
 
HaeII r_GCGC'y

Cuts at:      0     149    1436    1801    2199 
   Size:        149    1287     365     398

  Fragments arranged by size: 

                1287     398     365     149
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       9      41     870     902    1206    1238    1277    1308 
   Size:          9      32     829      32     304      32      39      31

Cuts at:   1308    1309    1340    1660    1692    2199 
   Size:          1      31     320      32     507

  Fragments arranged by size: 

                 829     507     320     304      39      32      32      32
                  32      31      31       9       1
 
HindIII A'AGCT_T

Cuts at:      0    1890    2199 
   Size:       1890     309
 
KasI G'GCGC_C

Cuts at:      0     145    1432    2199 
   Size:        145    1287     767

  Fragments arranged by size: 

                1287     767     145
 
KpnI G_GTAC'C

Cuts at:      0    2004    2199 
   Size:       2004     195
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     464    1497    1840    2199 
   Size:        185     279    1033     343     359

  Fragments arranged by size: 

                1033     359     343     279     185
 
MscI TGG'CCA

Cuts at:      0     154    2199 
   Size:        154    2045
 
MspA1I CmG'CkG

Cuts at:      0     131     285     780    1453    1565    1953    1998    2047 
   Size:        131     154     495     673     112     388      45      49

Cuts at:   2047    2199 
   Size:        152

  Fragments arranged by size: 

                 673     495     388     154     152     131     112      49
                  45
 
NaeI GCC'GGC

Cuts at:      0     973    2199 
   Size:        973    1226
 
NarI GG'CG_CC

Cuts at:      0     146    1433    2199 
   Size:        146    1287     766

  Fragments arranged by size: 

                1287     766     146
 
NcoI C'CATG_G

Cuts at:      0     119     935    2199 
   Size:        119     816    1264

  Fragments arranged by size: 

                1264     816     119
 
NgoMIV G'CCGG_C

Cuts at:      0     971    2199 
   Size:        971    1228
 
NsiI A_TGCA'T

Cuts at:      0     761    2199 
   Size:        761    1438
 
NspI r_CATG'y

Cuts at:      0     426    1704    2199 
   Size:        426    1278     495

  Fragments arranged by size: 

                1278     495     426
 
PasI CC'CwG_GG

Cuts at:      0     309     789    1875    2199 
   Size:        309     480    1086     324

  Fragments arranged by size: 

                1086     480     324     309
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     288    2199 
   Size:        288    1911
 
PmlI CAC'GTG

Cuts at:      0    1710    2007    2199 
   Size:       1710     297     192

  Fragments arranged by size: 

                1710     297     192
 
PshAI GACnn'nnGTC

Cuts at:      0    1755    2199 
   Size:       1755     444
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1984    2016    2199 
   Size:       1984      32     183

  Fragments arranged by size: 

                1984     183      32
 
PvuII CAG'CTG

Cuts at:      0     285     780    1453    1953    2199 
   Size:        285     495     673     500     246

  Fragments arranged by size: 

                 673     500     495     285     246
 
SacII CC_GC'GG

Cuts at:      0     132    2048    2199 
   Size:        132    1916     151

  Fragments arranged by size: 

                1916     151     132
 
ScaI AGT'ACT

Cuts at:      0     837    2199 
   Size:        837    1362
 
SfoI GGC'GCC

Cuts at:      0     147    1434    2199 
   Size:        147    1287     765

  Fragments arranged by size: 

                1287     765     147
 
SmlI C'TyrA_G

Cuts at:      0     995    1502    2199 
   Size:        995     507     697

  Fragments arranged by size: 

                 995     697     507
 
SphI G_CATG'C

Cuts at:      0    1704    2199 
   Size:       1704     495
 
StuI AGG'CCT

Cuts at:      0    1910    2199 
   Size:       1910     289
 
StyI C'CwwG_G

Cuts at:      0     119     155     342     935    1493    1769    2199 
   Size:        119      36     187     593     558     276     430

  Fragments arranged by size: 

                 593     558     430     276     187     119      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1044    2101    2199 
   Size:       1044    1057      98

  Fragments arranged by size: 

                1057    1044      98
 
TatI w'GTAC_w

Cuts at:      0     683     835    2129    2199 
   Size:        683     152    1294      70

  Fragments arranged by size: 

                1294     683     152      70
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2026    2058    2199 
   Size:       2026      32     141

  Fragments arranged by size: 

                2026     141      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2088    2199 
   Size:       2088     111
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     147    1068    1335    2199 
   Size:        147     921     267     864

  Fragments arranged by size: 

                 921     864     267     147
 
ZraI GAC'GTC

Cuts at:      0      66    2199 
   Size:         66    2133
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AlfI    AlwNI     ApoI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRV
     FalI    HaeII    Hin4I  HindIII     KasI     KpnI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI
    PflMI     PmlI    PshAI     PsrI    PvuII    SacII     ScaI     SfoI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BcgI
    BciVI     BclI     BmtI    BsaWI    BsiWI    BsmBI    BspEI   BssHII
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRI     FseI     FspI
    FspAI   HincII     HpaI     MfeI     MluI     MslI     NdeI     NheI
     NotI     NruI     PacI     PciI     PfoI     PmeI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XhoI
     XmaI     XmnI
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