Restriction map for pF1KSDA1029
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
Alw44I      AocI        ApaLI       AscI        AseI        
AsnI        Asp700I     AviII       AvrII       BbrPI       
BcgI        BclI        BfrI        BglII       BlnI        
BsaAI       BsaWI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        EagI        EclXI       Eco52I      Eco81I      
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        MfeI        MluI        MroI        
MseI        MunI        NaeI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          661  GTCTAC               GT^[AC][GT]AC
AcsI          348  GAATTC               [GA]^AATT[CT]
AflIII       2576  ACGTGT               A^C[GA][CT]GT
AhdI         1042  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2201  AGCGCT               AGC^GCT
ApaI         2115  GGGCCC               GGGCC^C
ApoI          348  GAATTC               [GA]^AATT[CT]
AspEI        1042  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1444  GAGCTC               G[AT]GC[AT]^C
AspI          969  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         731  GGATCC               G^GATCC
BciVI        1029  GTATCC               GTATCC(6/5)
BlpI         1441  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1441  GCTGAGC              GC^T[ACGT]AGC
BsaBI         283  GATGTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        2317  CGGTCG               CG[GA][CT]^CG
BsiHKAI      1444  GAGCTC               G[AT]GC[AT]^C
BsrBI        2556  CCGCTC               CCGCTC(-3/-3)
BstEII       1164  GGTGACC              G^GT[ACGT]ACC
BstPI        1164  GGTGACC              G^GT[ACGT]ACC
CelII        1441  GCTGAGC              GC^T[ACGT]AGC
Eam1105I     1042  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           64  GAAGAG               CTCTTC(1/4)
Eco47III     2201  AGCGCT               AGC^GCT
EcoO65I      1164  GGTGACC              G^GT[ACGT]ACC
EcoRI         348  GAATTC               G^AATTC
Ksp632I        64  GAAGAG               CTCTTC(1/4)
MaeII        2576  ACGT                 A^CGT
MamI          283  GATGTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI           22  GCTAGC               G^CTAGC
NspI         2402  GCATGC               [GA]CATG^[CT]
PflFI         969  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1658  GGGTCCT              [GA]G^G[AT]CC[CT]
SacI         1444  GAGCTC               GAGCT^C
SexAI        1790  ACCTGGT              A^CC[AT]GGT
SphI         2402  GCATGC               GCATG^C
TfiI         1173  GAATC                G^A[AT]TC
Tth111I       969  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

Acc65I         69  GGTACC               G^GTACC
             1344  GGTACC              
AlwNI         319  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              383  CAGGGCCTG           
Asp718I        69  GGTACC               G^GTACC
             1344  GGTACC              
BalI           50  TGGCCA               TGG^CCA
              686  TGGCCA              
BbsI         1050  GTCTTC               GAAGAC(2/6)
             1491  GTCTTC              
BbvCI         178  GCTGAGG              CCTCAGC(-5/-2)
              439  GCTGAGG             
BpuAI        1050  GTCTTC               GAAGAC(2/6)
             1491  GTCTTC              
BsmBI         783  GAGACG               CGTCTC(1/5)
              924  GAGACG              
BsmI           96  GAATGC               GAATGC(1/-1)
             1094  GCATTC              
BspMI        1643  ACCTGC               ACCTGC(4/8)
             1802  ACCTGC              
BssSI        1307  CTCGTG               CACGAG(-5/-1)
             2190  CTCGTG              
BstXI         752  CCAGCCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1778  CCAGCTCCTTGG        
BstYI         731  GGATCC               [GA]^GATC[CT]
             1188  GGATCT              
DraIII        405  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1304  CACCTCGTG           
HincII        205  GTCAAC               GT[CT]^[GA]AC
              706  GTCAAC              
HindII        205  GTCAAC               GT[CT]^[GA]AC
              706  GTCAAC              
KpnI           69  GGTACC               GGTAC^C
             1344  GGTACC              
MflI          731  GGATCC               [GA]^GATC[CT]
             1188  GGATCT              
MluNI          50  TGGCCA               TGG^CCA
              686  TGGCCA              
MscI           50  TGGCCA               TGG^CCA
              686  TGGCCA              
MslI          597  CACCCTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1902  CACTGCCGTG          
NcoI          878  CCATGG               C^CATGG
             1635  CCATGG              
PflMI         370  CCATCTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1952  CCAGGGCGTGG         
SmaI         1576  CCCGGG               CCC^GGG
             2112  CCCGGG              
SmlI         1366  CTCAAG               C^T[CT][GA]AG
             2668  CTCAAG              
Tsp509I       349  AATT                 ^AATT
              468  AATT                
Van91I        370  CCATCTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1952  CCAGGGCGTGG         
XcmI          593  CCAACACCCTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              977  CCACAGCCCCTCTGG     
XhoII         731  GGATCC               [GA]^GATC[CT]
             1188  GGATCT              
XmaCI        1576  CCCGGG               C^CCGGG
             2112  CCCGGG              
XmaI         1576  CCCGGG               C^CCGGG
             2112  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaI        AvaII       BanI        BanII       
BbeI        BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        KspI        MaeI        MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        StuI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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