Restriction map for pF1KSDA1020
 (Circular) (Six-base) MAPSORT of: pF1KSDA1020.seq  Check: 8974  from: 1  to: 4982



pF1KSDA1020  4982 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1955    1955 
   Size:       4982
 
AatII G_ACGT'C

Cuts at:    269     269 
   Size:       4982
 
AccI GT'mk_AC

Cuts at:   2020    2020 
   Size:       4982
 
Acc65I G'GTAC_C

Cuts at:   1998    1998 
   Size:       4982
 
AclI AA'CG_TT

Cuts at:   4822    4822 
   Size:       4982
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    456    1819    2807    3239    3986    4254     456 
   Size:       1363     988     432     747     268    1184

  Fragments arranged by size: 

                1363    1184     988     747     432     268
 
AflIII A'CryG_T

Cuts at:   1400    1771    2334    3438    4415    1400 
   Size:        371     563    1104     977    1967

  Fragments arranged by size: 

                1967    1104     977     563     371
 
AgeI A'CCGG_T

Cuts at:   3370    3370 
   Size:       4982
 
AleI CACnn'nnGTG

Cuts at:    150    1865     150 
   Size:       1715    3267
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    457     543    1514     457 
   Size:         86     971    3925

  Fragments arranged by size: 

                3925     971      86
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1516    1548    1516 
   Size:         32    4950
 
AlwNI CAG_nnn'CTG

Cuts at:    222     618     858    2287    3854     222 
   Size:        396     240    1429    1567    1350

  Fragments arranged by size: 

                1567    1429    1350     396     240
 
ApaI G_GGCC'C

Cuts at:    140     188     303     681    1196    1286     140 
   Size:         48     115     378     515      90    3836

  Fragments arranged by size: 

                3836     515     378     115      90      48
 
ApaLI G'TGCA_C

Cuts at:   1870    3752    1870 
   Size:       1882    3100
 
ApoI r'AATT_y

Cuts at:   1986    4335    4591    1986 
   Size:       2349     256    2377

  Fragments arranged by size: 

                2377    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4982
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4982
 
AvaI C'yCGr_G

Cuts at:    511    1209    1875    2002     511 
   Size:        698     666     127    3491

  Fragments arranged by size: 

                3491     698     666     127
 
BamHI G'GATC_C

Cuts at:   2007    2007 
   Size:       4982
 
BanI G'GyrC_C

Cuts at:   1024    1086    1573    1762    1998    2412    2660    2695    1024 
   Size:         62     487     189     236     414     248      35    3311

  Fragments arranged by size: 

                3311     487     414     248     236     189      62      35
            
 
BanII G_rGCy'C

Cuts at:    140     188     303     467     659     681     698    1040    1196 
   Size:         48     115     164     192      22      17     342     156

Cuts at:   1196    1286    1298    1631    1996    3026     140 
   Size:         90      12     333     365    1030    2096

  Fragments arranged by size: 

                2096    1030     365     342     333     192     164     156
                 115      90      48      22      17      12
 
BbeI G_GCGC'C

Cuts at:   1090    2416    2664    1090 
   Size:       1326     248    3408

  Fragments arranged by size: 

                3408    1326     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1352    1666    1705    1352 
   Size:        314      39    4629

  Fragments arranged by size: 

                4629     314      39
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1108    1142    1108 
   Size:         34    4948
 
BciVI GTATCCnnnnn_n'

Cuts at:   2880    3641    4982    2880 
   Size:        761    1341    2880

  Fragments arranged by size: 

                2880    1341     761
 
BclI T'GATC_A

Cuts at:   2502    2502 
   Size:       4982
 
BglI GCCn_nnn'nGGC

Cuts at:    788     959    1095    1621    1793     788 
   Size:        171     136     526     172    3977

  Fragments arranged by size: 

                3977     526     172     171     136
 
BglII A'GATC_T

Cuts at:    410    2497    4534     410 
   Size:       2087    2037     858

  Fragments arranged by size: 

                2087    2037     858
 
BlpI GC'TnA_GC

Cuts at:    943    1625    2094     943 
   Size:        682     469    3831

  Fragments arranged by size: 

                3831     682     469
 
Bme1580I G_kGCm'C

Cuts at:    140     188     303     681    1027    1196    1286    1874    2607 
   Size:         48     115     378     346     169      90     588     733

Cuts at:   2607    2700    3756     140 
   Size:         93    1056    1366

  Fragments arranged by size: 

                1366    1056     733     588     378     346     169     115
                  93      90      48
 
BmrI ACTGGGnnnn_n'

Cuts at:   1601    2194    2275    2610    4754    1601 
   Size:        593      81     335    2144    1829

  Fragments arranged by size: 

                2144    1829     593     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3349    3349 
   Size:       4982
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    487    1069    1102     487 
   Size:        582      33    4367

  Fragments arranged by size: 

                4367     582      33
 
Bpu10I CC'TnA_GC

Cuts at:   1072    1072 
   Size:       4982
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    526    2158    3037    3529    3827     526 
   Size:       1632     879     492     298    1681

  Fragments arranged by size: 

                1681    1632     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4686    4686 
   Size:       4982
 
BsaAI yAC'GTr

Cuts at:   1506    1974    2335    1506 
   Size:        468     361    4153

  Fragments arranged by size: 

                4153     468     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2496    4533       6 
   Size:       2490    2037     455

  Fragments arranged by size: 

                2490    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    266    1087    2413    2661    3363    4859     266 
   Size:        821    1326     248     702    1496     389

  Fragments arranged by size: 

                1496    1326     821     702     389     248
 
BsaWI w'CCGG_w

Cuts at:    149    2171    2692    3370    3644    3791    4202     149 
   Size:       2022     521     678     274     147     411     929

  Fragments arranged by size: 

                2022     929     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    472    1231    1330    1333    1336    1594    1597     472 
   Size:        759      99       3       3     258       3    3857

  Fragments arranged by size: 

                3857     759     258      99       3       3       3
 
BseYI C'CCAG_C

Cuts at:    202     445     783     858     938    1017    1480    1674    1922 
   Size:        243     338      75      80      79     463     194     248

Cuts at:   1922    2407    3165    3742    4238     202 
   Size:        485     758     577     496     946

  Fragments arranged by size: 

                 946     758     577     496     485     463     338     248
                 243     194      80      79      75
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    337     337 
   Size:       4982
 
BsiEI CG_ry'CG

Cuts at:    104    2524    2570    3778     104 
   Size:       2420      46    1208    1308

  Fragments arranged by size: 

                2420    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    698    1862    1874    1996    2774    2964    3756     698 
   Size:       1164      12     122     778     190     792    1924

  Fragments arranged by size: 

                1924    1164     792     778     190     122      12
 
BsmI GAATG_Cn'

Cuts at:   1457    1457 
   Size:       4982
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1417    1417 
   Size:       4982
 
Bsp1286I G_dGCh'C

Cuts at:    140     188     303     467     659     681     698    1027    1040 
   Size:         48     115     164     192      22      17     329      13

Cuts at:   1040    1196    1286    1298    1631    1862    1874    1996    2607 
   Size:        156      90      12     333     231      12     122     611

Cuts at:   2607    2700    2774    2964    3026    3756     140 
   Size:         93      74     190      62     730    1366

  Fragments arranged by size: 

                1366     730     611     333     329     231     192     190
                 164     156     122     115      93      90      74      62
                  48      22      17      13      12      12
 
BspEI T'CCGG_A

Cuts at:   4202    4202 
   Size:       4982
 
BspHI T'CATG_A

Cuts at:   4158    4158 
   Size:       4982
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1955    2032    2246    2548    2929    1955 
   Size:         77     214     302     381    4008

  Fragments arranged by size: 

                4008     381     302     214      77
 
BsrBI CCG'CTC

Cuts at:     50    1179    1361    3274    3328    4812    4977      50 
   Size:       1129     182    1913      54    1484     165      55

  Fragments arranged by size: 

                1913    1484    1129     182     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2894    2894 
   Size:       4982
 
BsrFI r'CCGG_y

Cuts at:    550    1406    2980    3161    3370     550 
   Size:        856    1574     181     209    2162

  Fragments arranged by size: 

                2162    1574     856     209     181
 
BssSI C'ACGA_G

Cuts at:    658    1693    3253    3611     658 
   Size:       1035    1560     358    2029

  Fragments arranged by size: 

                2029    1560    1035     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    222    1463    1609    2359     222 
   Size:       1241     146     750    2845

  Fragments arranged by size: 

                2845    1241     750     146
 
BstBI TT'CG_AA

Cuts at:    110    3343     110 
   Size:       3233    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:    591    1047     591 
   Size:        456    4526
 
BstYI r'GATC_y

Cuts at:      1     410    1044    2007    2474    2497    2832    3078    4090 
   Size:        409     634     963     467      23     335     246    1012

Cuts at:   4090    4176    4188    4534       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     963     634     467     449     409     346     335
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    125     623    1040     125 
   Size:        498     417    4067

  Fragments arranged by size: 

                4067     498     417
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1829    2932    3113    3276    1829 
   Size:       1103     181     163    3535

  Fragments arranged by size: 

                3535    1103     181     163
 
BtsI GCAGTG_nn'

Cuts at:    485    1572    2225     485 
   Size:       1087     653    3242

  Fragments arranged by size: 

                3242    1087     653
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1719    1754    1719 
   Size:         35    4947
 
DraI TTT'AAA

Cuts at:   1981    1981 
   Size:       4982
 
DraIII CAC_nnn'GTG

Cuts at:   1609    1609 
   Size:       4982
 
DrdI GACnn_nn'nnGTC

Cuts at:   2688    3546    2688 
   Size:        858    4124
 
EaeI y'GGCC_r

Cuts at:   1380    2567    2741    3132    3159    4504    1380 
   Size:       1187     174     391      27    1345    1858

  Fragments arranged by size: 

                1858    1345    1187     391     174      27
 
EagI C'GGCC_G

Cuts at:   2567    2567 
   Size:       4982
 
EarI CTCTTCn'nnn_

Cuts at:   1102    1520    1538    3005    3215    1102 
   Size:        418      18    1467     210    2869

  Fragments arranged by size: 

                2869    1467     418     210      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3190    3500    3646    4792    3190 
   Size:        310     146    1146    3380

  Fragments arranged by size: 

                3380    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    696    1994     696 
   Size:       1298    3684
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    456     487    1069    1102    1819    2807    3239    3986    4254 
   Size:         31     582      33     717     988     432     747     268

Cuts at:   4254     456 
   Size:       1184

  Fragments arranged by size: 

                1184     988     747     717     582     432     268      33
                  31
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1081    1081 
   Size:       4982
 
EcoO109I rG'GnC_Cy

Cuts at:    137     184     677     910    1192    1201    1283    1550    1593 
   Size:         47     493     233     282       9      82     267      43

Cuts at:   1593    1671    2121     137 
   Size:         78     450    2998

  Fragments arranged by size: 

                2998     493     450     282     267     233      82      78
                  47      43       9
 
EcoRI G'AATT_C

Cuts at:   1986    1986 
   Size:       4982
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3001    3001 
   Size:       4982
 
FspI TGC'GCA

Cuts at:   1355    1502    1951    2763    1355 
   Size:        147     449     812    3574

  Fragments arranged by size: 

                3574     812     449     147
 
FspAI rTGC'GCAy

Cuts at:   1502    1502 
   Size:       4982
 
HaeII r_GCGC'y

Cuts at:   1090    1394    2416    2664    3686    4665    1090 
   Size:        304    1022     248    1022     979    1407

  Fragments arranged by size: 

                1407    1022    1022     979     304     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1414    1446    1582    1614    2824    2854    2856    2886    1414 
   Size:         32     136      32    1210      30       2      30    3510

  Fragments arranged by size: 

                3510    1210     136      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2021    2021 
   Size:       4982
 
KasI G'GCGC_C

Cuts at:   1086    2412    2660    1086 
   Size:       1326     248    3408

  Fragments arranged by size: 

                3408    1326     248
 
KpnI G_GTAC'C

Cuts at:   2002    2002 
   Size:       4982
 
MluI A'CGCG_T

Cuts at:   1771    4415    1771 
   Size:       2644    2338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3653    3837    3653 
   Size:        184    4798
 
MscI TGG'CCA

Cuts at:   2743    2743 
   Size:       4982
 
MslI CAynn'nnrTG

Cuts at:    150     274     277     382     589    1865    1867    2971    3098 
   Size:        124       3     105     207    1276       2    1104     127

Cuts at:   3098     150 
   Size:       2034

  Fragments arranged by size: 

                2034    1276    1104     207     127     124     105       3
                   2
 
MspA1I CmG'CkG

Cuts at:    231     507     615     787     839     942    1379    1902    2093 
   Size:        276     108     172      52     103     437     523     191

Cuts at:   2093    2407    2767    3780    4025     231 
   Size:        314     360    1013     245    1188

  Fragments arranged by size: 

                1188    1013     523     437     360     314     276     245
                 191     172     108     103      52
 
NaeI GCC'GGC

Cuts at:    552    3163     552 
   Size:       2611    2371
 
NarI GG'CG_CC

Cuts at:   1087    2413    2661    1087 
   Size:       1326     248    3408

  Fragments arranged by size: 

                3408    1326     248
 
NcoI C'CATG_G

Cuts at:    125    1040     125 
   Size:        915    4067
 
NgoMIV G'CCGG_C

Cuts at:    550    3161     550 
   Size:       2611    2371
 
NspI r_CATG'y

Cuts at:   1404    1782    1830    1866    1956    2035    2535    3442    1404 
   Size:        378      48      36      90      79     500     907    2944

  Fragments arranged by size: 

                2944     907     500     378      90      79      48      36
            
 
PciI A'CATG_T

Cuts at:   1400    3438    1400 
   Size:       2038    2944
 
PmeI GTTT'AAAC

Cuts at:   1981    1981 
   Size:       4982
 
PmlI CAC'GTG

Cuts at:   1506    1506 
   Size:       4982
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4121    4153    4121 
   Size:         32    4950
 
PpuMI rG'GwC_Cy

Cuts at:    910    1201    1593    1671     910 
   Size:        291     392      78    4221

  Fragments arranged by size: 

                4221     392     291      78
 
PspOMI G'GGCC_C

Cuts at:    136     184     299     677    1192    1282     136 
   Size:         48     115     378     515      90    3836

  Fragments arranged by size: 

                3836     515     378     115      90      48
 
PstI C_TGCA'G

Cuts at:    230     817    2029    2714     230 
   Size:        587    1212     685    2498

  Fragments arranged by size: 

                2498    1212     685     587
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4982
 
PvuII CAG'CTG

Cuts at:    231     507     787     942    1902    2407    2767     231 
   Size:        276     280     155     960     505     360    2446

  Fragments arranged by size: 

                2446     960     505     360     280     276     155
 
RsrII CG'GwC_CG

Cuts at:   3177    3177 
   Size:       4982
 
SacI G_AGCT'C

Cuts at:    698    1996     698 
   Size:       1298    3684
 
SalI G'TCGA_C

Cuts at:   2019    2019 
   Size:       4982
 
SanDI GG'GwC_CC

Cuts at:   1201    1201 
   Size:       4982
 
SapI GCTCTTCn'nnn_

Cuts at:   1102    1520    3005    3215    1102 
   Size:        418    1485     210    2869

  Fragments arranged by size: 

                2869    1485     418     210
 
SbfI CC_TGCA'GG

Cuts at:   2029    2029 
   Size:       4982
 
ScaI AGT'ACT

Cuts at:   4499    4499 
   Size:       4982
 
SfcI C'TryA_G

Cuts at:     33     226     813    2025    2710    3703    3894      33 
   Size:        193     587    1212     685     993     191    1121

  Fragments arranged by size: 

                1212    1121     993     685     587     193     191
 
SfoI GGC'GCC

Cuts at:   1088    2414    2662    1088 
   Size:       1326     248    3408

  Fragments arranged by size: 

                3408    1326     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4982
 
SmaI CCC'GGG

Cuts at:   1211    2004    1211 
   Size:        793    4189
 
SmlI C'TyrA_G

Cuts at:    541    2137    3052    3544    3806     541 
   Size:       1596     915     492     262    1717

  Fragments arranged by size: 

                1717    1596     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1974    1974 
   Size:       4982
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4982
 
SphI G_CATG'C

Cuts at:   1830    1956    2035    2535    1830 
   Size:        126      79     500    4277

  Fragments arranged by size: 

                4277     500     126      79
 
StyI C'CwwG_G

Cuts at:    125     140     248    1040    1196    1469    2116    2470     125 
   Size:         15     108     792     156     273     647     354    2637

  Fragments arranged by size: 

                2637     792     647     354     273     156     108      15
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1620    1638    1936    2565    2698    3157    3366    1620 
   Size:         18     298     629     133     459     209    3236

  Fragments arranged by size: 

                3236     629     459     298     209     133      18
 
TatI w'GTAC_w

Cuts at:   4497    4497 
   Size:       4982
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    894     926    1706    1738    4499    4531     894 
   Size:         32     780      32    2761      32    1345

  Fragments arranged by size: 

                2761    1345     780      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    385    1428    2779     385 
   Size:       1043    1351    2588

  Fragments arranged by size: 

                2588    1351    1043
 
XbaI T'CTAG_A

Cuts at:   2013    2013 
   Size:       4982
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1254    2477    1254 
   Size:       1223    3759
 
XmaI C'CCGG_G

Cuts at:   1209    2002    1209 
   Size:        793    4189
 
XmnI GAAnn'nnTTC

Cuts at:    728    1804     728 
   Size:       1076    3906
 
ZraI GAC'GTC

Cuts at:    267     267 
   Size:       4982
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PmlI
     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AscI    AvrII     BaeI    BbvCI    BfrBI
    BmgBI     BmtI    BsaXI    BsiWI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI    EcoRV     FseI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
    PshAI     PsiI    PspXI     PsrI    SacII    SexAI     SfiI    SgrAI
     SrfI     SspI     StuI     SwaI     XhoI
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