Restriction map for pF1KSDA0980
 (Linear) (Six-base) MAPSORT of: KIAA0980.seq  Check: 9261  from: 1  to: 4146



KIAA0980.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1164    2195    4146 
   Size:       1164    1031    1951

  Fragments arranged by size: 

                1951    1164    1031
 
AatII G_ACGT'C

Cuts at:      0    3203    4146 
   Size:       3203     943
 
AccI GT'mk_AC

Cuts at:      0      44    4146 
   Size:         44    4102
 
Acc65I G'GTAC_C

Cuts at:      0    1325    4146 
   Size:       1325    2821
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     771    1026    1501    1576    1606    1672    2374    3290 
   Size:        771     255     475      75      30      66     702     916

Cuts at:   3290    3787    3925    3967    4009    4015    4146 
   Size:        497     138      42      42       6     131

  Fragments arranged by size: 

                 916     771     702     497     475     255     138     131
                  75      66      42      42      30       6
 
AfeI AGC'GCT

Cuts at:      0    3149    4146 
   Size:       3149     997
 
AflII C'TTAA_G

Cuts at:      0     109    4146 
   Size:        109    4037
 
AflIII A'CryG_T

Cuts at:      0     375    2527    3222    4146 
   Size:        375    2152     695     924

  Fragments arranged by size: 

                2152     924     695     375
 
AleI CACnn'nnGTG

Cuts at:      0    2868    4146 
   Size:       2868    1278
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2157    2384    3134    4146 
   Size:       2157     227     750    1012

  Fragments arranged by size: 

                2157    1012     750     227
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1621    1653    3958    3990    4146 
   Size:       1621      32    2305      32     156

  Fragments arranged by size: 

                2305    1621     156      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      53      93     132     660     981    1035    1155    1218 
   Size:         53      40      39     528     321      54     120      63

Cuts at:   1218    2154    2484    3549    3642    4146 
   Size:        936     330    1065      93     504

  Fragments arranged by size: 

                1065     936     528     504     330     321     120      93
                  63      54      53      40      39
 
ApaI G_GGCC'C

Cuts at:      0    1086    2459    4146 
   Size:       1086    1373    1687

  Fragments arranged by size: 

                1687    1373    1086
 
ApoI r'AATT_y

Cuts at:      0     193     769    4146 
   Size:        193     576    3377

  Fragments arranged by size: 

                3377     576     193
 
AvaI C'yCGr_G

Cuts at:      0     697     824    2110    2410    2813    2996    4146 
   Size:        697     127    1286     300     403     183    1150

  Fragments arranged by size: 

                1286    1150     697     403     300     183     127
 
BanI G'GyrC_C

Cuts at:      0    1325    1430    2133    2965    3007    3759    3824    4057 
   Size:       1325     105     703     832      42     752      65     233

Cuts at:   4057    4146 
   Size:         89

  Fragments arranged by size: 

                1325     832     752     703     233     105      89      65
                  42
 
BanII G_rGCy'C

Cuts at:      0     556     689     800    1086    1100    1619    1667    1768 
   Size:        556     133     111     286      14     519      48     101

Cuts at:   1768    2045    2250    2459    2671    2814    3213    3708    4146 
   Size:        277     205     209     212     143     399     495     438

  Fragments arranged by size: 

                 556     519     495     438     399     286     277     212
                 209     205     143     133     111     101      48      14
            
 
BbeI G_GCGC'C

Cuts at:      0    1434    3011    3828    4061    4146 
   Size:       1434    1577     817     233      85

  Fragments arranged by size: 

                1577    1434     817     233      85
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     215     260     722     809    3203    4146 
   Size:        215      45     462      87    2394     943

  Fragments arranged by size: 

                2394     943     462     215      87      45
 
BbvCI CC'TCA_GC

Cuts at:      0    1442    2210    3718    4146 
   Size:       1442     768    1508     428

  Fragments arranged by size: 

                1508    1442     768     428
 
BclI T'GATC_A

Cuts at:      0     972    4146 
   Size:        972    3174
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1808    2522    2581    4146 
   Size:       1808     714      59    1565

  Fragments arranged by size: 

                1808    1565     714      59
 
BglII A'GATC_T

Cuts at:      0    1022    4146 
   Size:       1022    3124
 
BlpI GC'TnA_GC

Cuts at:      0     426    1156    1339    1709    3796    4146 
   Size:        426     730     183     370    2087     350

  Fragments arranged by size: 

                2087     730     426     370     350     183
 
Bme1580I G_kGCm'C

Cuts at:      0    1086    2352    2459    2527    2968    3764    4146 
   Size:       1086    1266     107      68     441     796     382

  Fragments arranged by size: 

                1266    1086     796     441     382     107      68
 
BmgBI CAC'GTC

Cuts at:      0    1425    4146 
   Size:       1425    2721
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3484    4146 
   Size:       3484     662
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2997    4146 
   Size:       2997    1149
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     267     457     673     903     921    1090    1240 
   Size:        142     125     190     216     230      18     169     150

Cuts at:   1240    1264    1303    1336    1435    1594    1618    1820    1927 
   Size:         24      39      33      99     159      24     202     107

Cuts at:   1927    2035    2056    2302    2356    2362    2818    2828    2976 
   Size:        108      21     246      54       6     456      10     148

Cuts at:   2976    3034    3061    3172    3235    3271    3571    4146 
   Size:         58      27     111      63      36     300     575

  Fragments arranged by size: 

                 575     456     300     246     230     216     202     190
                 169     159     150     148     142     125     111     108
                 107      99      63      58      54      39      36      33
                  27      24      24      21      18      10       6
 
Bpu10I CC'TnA_GC

Cuts at:      0      34     344     646    1442    2117    2210    3718    4146 
   Size:         34     310     302     796     675      93    1508     428

  Fragments arranged by size: 

                1508     796     675     428     310     302      93      34
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     784    1651    3697    4146 
   Size:        784     867    2046     449

  Fragments arranged by size: 

                2046     867     784     449
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     854    1903    2258    3130    4146 
   Size:        854    1049     355     872    1016

  Fragments arranged by size: 

                1049    1016     872     854     355
 
BsaAI yAC'GTr

Cuts at:      0    2530    4002    4146 
   Size:       2530    1472     144

  Fragments arranged by size: 

                2530    1472     144
 
BsaHI Gr'CG_yC

Cuts at:      0    1431    2846    3008    3200    3825    4058    4146 
   Size:       1431    1415     162     192     625     233      88

  Fragments arranged by size: 

                1431    1415     625     233     192     162      88
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1258    1288    1309    1339    1621    1651    1772    1802 
   Size:       1258      30      21      30     282      30     121      30

Cuts at:   1802    2407    2437    2443    2473    2519    2549    3007    3037 
   Size:        605      30       6      30      46      30     458      30

Cuts at:   3037    4146 
   Size:       1109

  Fragments arranged by size: 

                1258    1109     605     458     282     121      46      30
                  30      30      30      30      30      30      30      21
                   6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     883    1459    1645    2053    2299    2428    2431    2434 
   Size:        883     576     186     408     246     129       3       3

Cuts at:   2434    2623    3526    3568    3913    4057    4146 
   Size:        189     903      42     345     144      89

  Fragments arranged by size: 

                 903     883     576     408     345     246     189     186
                 144     129      89      42       3       3
 
BseYI C'CCAG_C

Cuts at:      0      98     621    1803    1901    2241    2829    2858    3449 
   Size:         98     523    1182      98     340     588      29     591

Cuts at:   3449    3734    4146 
   Size:        285     412

  Fragments arranged by size: 

                1182     591     588     523     412     340     285      98
                  98      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1631    2127    2922    3718    4146 
   Size:       1631     496     795     796     428

  Fragments arranged by size: 

                1631     796     795     496     428
 
BsiEI CG_ry'CG

Cuts at:      0    2894    4146 
   Size:       2894    1252
 
BsiHKAI G_wGCw'C

Cuts at:      0     481     919    1100    1619    1667    2045    2949    3413 
   Size:        481     438     181     519      48     378     904     464

Cuts at:   3413    3708    4029    4146 
   Size:        295     321     117

  Fragments arranged by size: 

                 904     519     481     464     438     378     321     295
                 181     117      48
 
BsmI GAATG_Cn'

Cuts at:      0    3622    4146 
   Size:       3622     524
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     437     740    2837    4146 
   Size:        437     303    2097    1309

  Fragments arranged by size: 

                2097    1309     437     303
 
Bsp1286I G_dGCh'C

Cuts at:      0     481     556     689     800     919    1086    1100    1619 
   Size:        481      75     133     111     119     167      14     519

Cuts at:   1619    1667    1768    2045    2250    2352    2459    2527    2671 
   Size:         48     101     277     205     102     107      68     144

Cuts at:   2671    2814    2949    2968    3213    3413    3708    3764    4029 
   Size:        143     135      19     245     200     295      56     265

Cuts at:   4029    4146 
   Size:        117

  Fragments arranged by size: 

                 519     481     295     277     265     245     205     200
                 167     144     143     135     133     119     117     111
                 107     102     101      75      68      56      48      19
                  14
 
BspHI T'CATG_A

Cuts at:      0     792    1100    4146 
   Size:        792     308    3046

  Fragments arranged by size: 

                3046     792     308
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1164    1688    1860    2195    3989    4146 
   Size:       1164     524     172     335    1794     157

  Fragments arranged by size: 

                1794    1164     524     335     172     157
 
BsrBI CCG'CTC

Cuts at:      0     875    1635    2257    2565    3879    4146 
   Size:        875     760     622     308    1314     267

  Fragments arranged by size: 

                1314     875     760     622     308     267
 
BsrFI r'CCGG_y

Cuts at:      0     338    1745    2889    4146 
   Size:        338    1407    1144    1257

  Fragments arranged by size: 

                1407    1257    1144     338
 
BssHII G'CGCG_C

Cuts at:      0    2382    4146 
   Size:       2382    1764
 
BssSI C'ACGA_G

Cuts at:      0     904    2047    4146 
   Size:        904    1143    2099

  Fragments arranged by size: 

                2099    1143     904
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     132    2484    3792    4146 
   Size:        132    2352    1308     354

  Fragments arranged by size: 

                2352    1308     354     132
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2576    2870    4146 
   Size:       2576     294    1276

  Fragments arranged by size: 

                2576    1276     294
 
BstYI r'GATC_y

Cuts at:      0     599    1022    4146 
   Size:        599     423    3124

  Fragments arranged by size: 

                3124     599     423
 
Bsu36I CC'TnA_GG

Cuts at:      0    1894    2884    3712    4146 
   Size:       1894     990     828     434

  Fragments arranged by size: 

                1894     990     828     434
 
BtgI C'CryG_G

Cuts at:      0      62    2103    2436    2697    2863    3652    4146 
   Size:         62    2041     333     261     166     789     494

  Fragments arranged by size: 

                2041     789     494     333     261     166      62
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3772    4146 
   Size:       3772     374
 
BtsI GCAGTG_nn'

Cuts at:      0    2542    3573    4146 
   Size:       2542    1031     573

  Fragments arranged by size: 

                2542    1031     573
 
DraI TTT'AAA

Cuts at:      0    3371    4146 
   Size:       3371     775
 
DraIII CAC_nnn'GTG

Cuts at:      0    1609    2158    3039    4020    4146 
   Size:       1609     549     881     981     126

  Fragments arranged by size: 

                1609     981     881     549     126
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1117    4146 
   Size:       1117    3029
 
EaeI y'GGCC_r

Cuts at:      0     327     983    2514    2566    2891    3776    4146 
   Size:        327     656    1531      52     325     885     370

  Fragments arranged by size: 

                1531     885     656     370     327     325      52
 
EagI C'GGCC_G

Cuts at:      0    2891    4146 
   Size:       2891    1255
 
EarI CTCTTCn'nnn_

Cuts at:      0       5     461     611     790     937    1228    2933    3122 
   Size:          5     456     150     179     147     291    1705     189

Cuts at:   3122    3869    3881    4146 
   Size:        747      12     265

  Fragments arranged by size: 

                1705     747     456     291     265     189     179     150
                 147      12       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1381    2230    3907    4146 
   Size:       1381     849    1677     239

  Fragments arranged by size: 

                1677    1381     849     239
 
EcoICRI GAG'CTC

Cuts at:      0    1098    1617    1665    2043    3706    4146 
   Size:       1098     519      48     378    1663     440

  Fragments arranged by size: 

                1663    1098     519     440     378      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     267     457     673     771     903     921    1026 
   Size:        142     125     190     216      98     132      18     105

Cuts at:   1026    1090    1240    1264    1303    1336    1435    1501    1576 
   Size:         64     150      24      39      33      99      66      75

Cuts at:   1576    1594    1606    1618    1672    1820    1927    2035    2056 
   Size:         18      12      12      54     148     107     108      21

Cuts at:   2056    2302    2356    2362    2374    2818    2828    2976    3034 
   Size:        246      54       6      12     444      10     148      58

Cuts at:   3034    3061    3172    3235    3271    3290    3571    3787    3925 
   Size:         27     111      63      36      19     281     216     138

Cuts at:   3925    3967    4009    4015    4146 
   Size:         42      42       6     131

  Fragments arranged by size: 

                 444     281     246     216     216     190     150     148
                 148     142     138     132     131     125     111     108
                 107     105      99      98      75      66      64      63
                  58      54      54      42      42      39      36      33
                  27      24      21      19      18      18      12      12
                  12      10       6       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1501    1624    2413    2709    3553    3625    4146 
   Size:       1501     123     789     296     844      72     521

  Fragments arranged by size: 

                1501     844     789     521     296     123      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0     861     978    1083    1215    1569    1677    1898    2073 
   Size:        861     117     105     132     354     108     221     175

Cuts at:   2073    2299    2456    2481    3059    4146 
   Size:        226     157      25     578    1087

  Fragments arranged by size: 

                1087     861     578     354     226     221     175     157
                 132     117     108     105      25
 
EcoRI G'AATT_C

Cuts at:      0     769    4146 
   Size:        769    3377
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     778    4146 
   Size:        778    3368
 
FseI GG_CCGG'CC

Cuts at:      0    2893    4146 
   Size:       2893    1253
 
HaeII r_GCGC'y

Cuts at:      0     426    1434    3011    3151    3828    4061    4146 
   Size:        426    1008    1577     140     677     233      85

  Fragments arranged by size: 

                1577    1008     677     426     233     140      85
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1258    1290    1772    1804    2443    2475    2701    2733 
   Size:       1258      32     482      32     639      32     226      32

Cuts at:   2733    3430    3462    4146 
   Size:        697      32     684

  Fragments arranged by size: 

                1258     697     684     639     482     226      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     183    3539    4146 
   Size:        183    3356     607

  Fragments arranged by size: 

                3356     607     183
 
HindIII A'AGCT_T

Cuts at:      0     112    1528    4146 
   Size:        112    1416    2618

  Fragments arranged by size: 

                2618    1416     112
 
HpaI GTT'AAC

Cuts at:      0     183    3539    4146 
   Size:        183    3356     607

  Fragments arranged by size: 

                3356     607     183
 
KasI G'GCGC_C

Cuts at:      0    1430    3007    3824    4057    4146 
   Size:       1430    1577     817     233      89

  Fragments arranged by size: 

                1577    1430     817     233      89
 
KpnI G_GTAC'C

Cuts at:      0    1329    4146 
   Size:       1329    2817
 
MluI A'CGCG_T

Cuts at:      0     375    4146 
   Size:        375    3771
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     313    2306    2405    4001    4146 
   Size:        313    1993      99    1596     145

  Fragments arranged by size: 

                1993    1596     313     145      99
 
MscI TGG'CCA

Cuts at:      0     985    2516    4146 
   Size:        985    1531    1630

  Fragments arranged by size: 

                1630    1531     985
 
MslI CAynn'nnrTG

Cuts at:      0    1105    2868    3532    4146 
   Size:       1105    1763     664     614

  Fragments arranged by size: 

                1763    1105     664     614
 
MspA1I CmG'CkG

Cuts at:      0      53     132     277     522     638    1185    1905    2088 
   Size:         53      79     145     245     116     547     720     183

Cuts at:   2088    2105    2334    2438    2791    3648    3840    3861    4146 
   Size:         17     229     104     353     857     192      21     285

  Fragments arranged by size: 

                 857     720     547     353     285     245     229     192
                 183     145     116     104      79      53      21      17
            
 
NaeI GCC'GGC

Cuts at:      0     340    2891    4146 
   Size:        340    2551    1255

  Fragments arranged by size: 

                2551    1255     340
 
NarI GG'CG_CC

Cuts at:      0    1431    3008    3825    4058    4146 
   Size:       1431    1577     817     233      88

  Fragments arranged by size: 

                1577    1431     817     233      88
 
NcoI C'CATG_G

Cuts at:      0    3652    4146 
   Size:       3652     494
 
NgoMIV G'CCGG_C

Cuts at:      0     338    2889    4146 
   Size:        338    2551    1257

  Fragments arranged by size: 

                2551    1257     338
 
NspI r_CATG'y

Cuts at:      0    2727    3226    4146 
   Size:       2727     499     920

  Fragments arranged by size: 

                2727     920     499
 
PciI A'CATG_T

Cuts at:      0    3222    4146 
   Size:       3222     924
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2593    4146 
   Size:       2593    1553
 
PmlI CAC'GTG

Cuts at:      0    2530    4146 
   Size:       2530    1616
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1621    1653    4146 
   Size:       1621      32    2493

  Fragments arranged by size: 

                2493    1621      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     861     978    1215    1569    1677    1898    2299    3059 
   Size:        861     117     237     354     108     221     401     760

Cuts at:   3059    4146 
   Size:       1087

  Fragments arranged by size: 

                1087     861     760     401     354     237     221     117
                 108
 
PspOMI G'GGCC_C

Cuts at:      0    1082    2455    4146 
   Size:       1082    1373    1691

  Fragments arranged by size: 

                1691    1373    1082
 
PspXI vC'TCGA_Gb

Cuts at:      0    2410    4146 
   Size:       2410    1736
 
PstI C_TGCA'G

Cuts at:      0     149     527     571    1439    1565    1685    2081    2093 
   Size:        149     378      44     868     126     120     396      12

Cuts at:   2093    2192    2363    3330    3647    4146 
   Size:         99     171     967     317     499

  Fragments arranged by size: 

                 967     868     499     396     378     317     171     149
                 126     120      99      44      12
 
PvuII CAG'CTG

Cuts at:      0      53     132     277     522    1185    1905    2088    2334 
   Size:         53      79     145     245     663     720     183     246

Cuts at:   2334    3648    3861    4146 
   Size:       1314     213     285

  Fragments arranged by size: 

                1314     720     663     285     246     245     213     183
                 145      79      53
 
SacI G_AGCT'C

Cuts at:      0    1100    1619    1667    2045    3708    4146 
   Size:       1100     519      48     378    1663     438

  Fragments arranged by size: 

                1663    1100     519     438     378      48
 
SacII CC_GC'GG

Cuts at:      0    2106    2439    4146 
   Size:       2106     333    1707

  Fragments arranged by size: 

                2106    1707     333
 
SanDI GG'GwC_CC

Cuts at:      0    2299    4146 
   Size:       2299    1847
 
SapI GCTCTTCn'nnn_

Cuts at:      0     611    2933    3869    4146 
   Size:        611    2322     936     277

  Fragments arranged by size: 

                2322     936     611     277
 
SbfI CC_TGCA'GG

Cuts at:      0    1439    1685    2081    4146 
   Size:       1439     246     396    2065

  Fragments arranged by size: 

                2065    1439     396     246
 
ScaI AGT'ACT

Cuts at:      0    3336    4146 
   Size:       3336     810
 
SexAI A'CCwGG_T

Cuts at:      0     857    3692    4016    4146 
   Size:        857    2835     324     130

  Fragments arranged by size: 

                2835     857     324     130
 
SfcI C'TryA_G

Cuts at:      0      45     145     523     567    1435    1498    1561    1681 
   Size:         45     100     378      44     868      63      63     120

Cuts at:   1681    2077    2089    2188    2359    2923    3326    3643    4146 
   Size:        396      12      99     171     564     403     317     503

  Fragments arranged by size: 

                 868     564     503     403     396     378     317     171
                 120     100      99      63      63      45      44      12
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2581    4146 
   Size:       2581    1565
 
SfoI GGC'GCC

Cuts at:      0    1432    3009    3826    4059    4146 
   Size:       1432    1577     817     233      87

  Fragments arranged by size: 

                1577    1432     817     233      87
 
SmaI CCC'GGG

Cuts at:      0    2815    2998    4146 
   Size:       2815     183    1148

  Fragments arranged by size: 

                2815    1148     183
 
SmlI C'TyrA_G

Cuts at:      0     109     697     763    1666    2410    3676    4146 
   Size:        109     588      66     903     744    1266     470

  Fragments arranged by size: 

                1266     903     744     588     470     109      66
 
SphI G_CATG'C

Cuts at:      0    2727    4146 
   Size:       2727    1419
 
SrfI GCCC'GGGC

Cuts at:      0    2998    4146 
   Size:       2998    1148
 
StuI AGG'CCT

Cuts at:      0    1721    2980    3391    4146 
   Size:       1721    1259     411     755

  Fragments arranged by size: 

                1721    1259     755     411
 
StyI C'CwwG_G

Cuts at:      0    1913    2273    3652    4146 
   Size:       1913     360    1379     494

  Fragments arranged by size: 

                1913    1379     494     360
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2161    2983    3011    4146 
   Size:       2161     822      28    1135

  Fragments arranged by size: 

                2161    1135     822      28
 
TatI w'GTAC_w

Cuts at:      0    3334    4146 
   Size:       3334     812
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1708    1740    2519    2551    3007    3039    4146 
   Size:       1708      32     779      32     456      32    1107

  Fragments arranged by size: 

                1708    1107     779     456      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3202    4146 
   Size:       3202     944
 
XbaI T'CTAG_A

Cuts at:      0     810    4146 
   Size:        810    3336
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2155    3147    3825    4146 
   Size:       2155     992     678     321

  Fragments arranged by size: 

                2155     992     678     321
 
XhoI C'TCGA_G

Cuts at:      0     697    2410    4146 
   Size:        697    1713    1736

  Fragments arranged by size: 

                1736    1713     697
 
XmaI C'CCGG_G

Cuts at:      0    2813    2996    4146 
   Size:       2813     183    1150

  Fragments arranged by size: 

                2813    1150     183
 
XmnI GAAnn'nnTTC

Cuts at:      0    4091    4146 
   Size:       4091      55
 
ZraI GAC'GTC

Cuts at:      0    3201    4146 
   Size:       3201     945
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AgeI     AhdI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BciVI    BfrBI     BmtI    BsaBI    BsaWI
    BsiWI    BspEI    BsrDI    BsrGI    BstBI   BstEII  BstZ17I     ClaI
    CspCI    EcoRV     FspI    FspAI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI    PshAI     PsiI
     PsrI     PvuI    RsrII     SalI     SgfI    SgrAI    SnaBI     SpeI
     SspI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com