Restriction map for
pF1KSDA0977
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcnI
BglI BglII BlnI BsaAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I Cfr10I CpoI DraII
DrdI Eam1105I Eco47III EcoNI EcoO109I
EcoRV FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 17 CGCG CG^CG
AccIII 2847 TCCGGA T^CCGGA
AcyI 28 GACGCC G[GA]^CG[CT]C
AfaI 1870 GTAC GT^AC
AlwNI 104 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1653 GGGCCC GGGCC^C
AseI 451 ATTAAT AT^TAAT
AsnI 451 ATTAAT AT^TAAT
Asp700I 1225 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2678 CTCGGG C^[CT]CG[GA]G
BcgI 3376 GCAATCCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3001 GTATCC GTATCC(6/5)
BclI 2385 TGATCA T^GATCA
BlpI 1038 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1038 GCTCAGC GC^T[ACGT]AGC
BsaHI 28 GACGCC G[GA]^CG[CT]C
BsaWI 2847 TCCGGA [AT]^CCGG[AT]
BseAI 2847 TCCGGA T^CCGGA
BsiEI 6 CGGCCG CG[GA][CT]^CG
BsoBI 2678 CTCGGG C^[CT]CG[GA]G
BspDI 298 ATCGAT AT^CGAT
BspEI 2847 TCCGGA T^CCGGA
BspHI 2421 TCATGA T^CATGA
BssSI 2279 CACGAG CACGAG(-5/-1)
BstAPI 2529 GCATCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3414 GGTTACC G^GT[ACGT]ACC
BstPI 3414 GGTTACC G^GT[ACGT]ACC
BstUI 17 CGCG CG^CG
CelII 1038 GCTCAGC GC^T[ACGT]AGC
ClaI 298 ATCGAT AT^CGAT
EagI 6 CGGCCG C^GGCCG
EclXI 6 CGGCCG C^GGCCG
Eco52I 6 CGGCCG C^GGCCG
EcoO65I 3414 GGTTACC G^GT[ACGT]ACC
FbaI 2385 TGATCA T^GATCA
HapII 2848 CCGG C^CGG
HgaI 28 GACGC GACGC(5/10)
Hin1I 28 GACGCC G[GA]^CG[CT]C
HpaII 2848 CCGG C^CGG
MaeII 170 ACGT A^CGT
MroI 2847 TCCGGA T^CCGGA
MspI 2848 CCGG C^CGG
MvnI 17 CGCG CG^CG
PshBI 451 ATTAAT AT^TAAT
RcaI 2421 TCATGA T^CATGA
RsaI 1870 GTAC GT^AC
SacI 2550 GAGCTC GAGCT^C
SapI 2482 GAAGAGC GCTCTTC(1/4)
SphI 2397 GCATGC GCATG^C
StuI 946 AGGCCT AGG^CCT
SwaI 2670 ATTTAAAT ATTT^AAAT
XmnI 1225 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 2046 CTTAAG C^TTAAG
2868 CTTAAG
AocI 1204 CCTGAGG CC^T[ACGT]AGG
1315 CCTGAGG
BfrI 2046 CTTAAG C^TTAAG
2868 CTTAAG
BseRI 2957 CTCCTC GAGGAG(10/8)
1207 GAGGAG
BsgI 3248 CTGCAC GTGCAG(16/14)
1898 GTGCAG
BspMI 2586 ACCTGC ACCTGC(4/8)
1024 GCAGGT
BsrDI 1508 GCAATG GCAATG(2/0)
1607 GCAATG
BstYI 2118 AGATCC [GA]^GATC[CT]
2136 AGATCC
Bsu36I 1204 CCTGAGG CC^T[ACGT]AGG
1315 CCTGAGG
DraI 381 TTTAAA TTT^AAA
2671 TTTAAA
DsaI 1538 CCATGG C^C[GA][CT]GG
2567 CCATGG
EaeI 6 CGGCCG [CT]^GGCC[GA]
3492 CGGCCA
Eco81I 1204 CCTGAGG CC^T[ACGT]AGG
1315 CCTGAGG
EcoT22I 497 ATGCAT ATGCA^T
2395 ATGCAT
HincII 652 GTCAAC GT[CT]^[GA]AC
1624 GTTGAC
HindII 652 GTCAAC GT[CT]^[GA]AC
1624 GTTGAC
MflI 2118 AGATCC [GA]^GATC[CT]
2136 AGATCC
NcoI 1538 CCATGG C^CATGG
2567 CCATGG
NsiI 497 ATGCAT ATGCA^T
2395 ATGCAT
SspI 1423 AATATT AAT^ATT
2653 AATATT
TaqI 299 TCGA T^CGA
2347 TCGA
TthHB8I 299 TCGA T^CGA
2347 TCGA
XhoII 2118 AGATCC [GA]^GATC[CT]
2136 AGATCC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AluI Alw44I AlwI
ApaLI ApoI AspHI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraIII EarI EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I