Restriction map for pF1KSDA0958
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AvrII       BbvCI       
BcgI        BfrI        BglI        BglII       BlnI        
BsaBI       BsgI        BsiWI       BsmI        Bsp1407I    
BspDI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      ClaI        DraI        
DraIII      DrdI        Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MseI        MslI        MspA1I      MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AatII         145  GACGTC               GACGT^C
AccII         846  CGCG                 CG^CG
AccIII        299  TCCGGA               T^CCGGA
AhdI          552  GACCAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         552  GACCAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1078  GAGCTC               G[AT]GC[AT]^C
AvaI         1039  CCCGAG               C^[CT]CG[GA]G
BalI          989  TGGCCA               TGG^CCA
BamHI         797  GGATCC               G^GATCC
BbeI          606  GGCGCC               GGCGC^C
BbrPI        1062  CACGTG               CAC^GTG
BciVI         702  GGATAC               GTATCC(6/5)
BclI          486  TGATCA               T^GATCA
BfaI          850  CTAG                 C^TAG
BlpI          590  GCTCAGC              GC^T[ACGT]AGC
BpmI         1075  CTGGAG               CTGGAG(16/14)
Bpu1102I      590  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1062  CACGTG               [CT]AC^GT[GA]
BsaI          550  GAGACC               GGTCTC(1/5)
BsaWI         299  TCCGGA               [AT]^CCGG[AT]
BseAI         299  TCCGGA               T^CCGGA
BsiHKAI      1078  GAGCTC               G[AT]GC[AT]^C
BsoBI        1039  CCCGAG               C^[CT]CG[GA]G
BspEI         299  TCCGGA               T^CCGGA
BspHI         956  TCATGA               T^CATGA
BsrFI         968  ACCGGC               [GA]^CCGG[CT]
BssSI         514  CACGAG               CACGAG(-5/-1)
BstUI         846  CGCG                 CG^CG
Cac8I        1139  GCCTGC               GC[ACGT]^[ACGT]GC
CelII         590  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        968  ACCGGC               [GA]^CCGG[CT]
CpoI          637  CGGTCCG              CG^G[AT]CCG
Eam1105I      552  GACCAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         735  CCTGCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       253  CCATGG               C^C[AT][AT]GG
FbaI          486  TGATCA               T^GATCA
HaeII         606  GGCGCC               [GA]GCGC^[CT]
HgaI          787  GACGC                GACGC(5/10)
HincII        148  GTCAAC               GT[CT]^[GA]AC
HindII        148  GTCAAC               GT[CT]^[GA]AC
HphI         1047  GGTGA                GGTGA(8/7)
KasI          606  GGCGCC               G^GCGCC
MaeI          850  CTAG                 C^TAG
MluNI         989  TGGCCA               TGG^CCA
MroI          299  TCCGGA               T^CCGGA
MscI          989  TGGCCA               TGG^CCA
MvnI          846  CGCG                 CG^CG
NarI          606  GGCGCC               GG^CGCC
NcoI          253  CCATGG               C^CATGG
PflMI         966  CCACCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1062  CACGTG               CAC^GTG
PmlI         1062  CACGTG               CAC^GTG
PpuMI         448  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          956  TCATGA               T^CATGA
RsrII         637  CGGTCCG              CG^G[AT]CCG
SacI         1078  GAGCTC               GAGCT^C
SapI         1018  GAAGAGC              GCTCTTC(1/4)
SfaNI         997  GATGC                GCATC(5/9)
SfcI          522  CTACAG               C^T[GA][CT]AG
SfoI          606  GGCGCC               GGC^GCC
StyI          253  CCATGG               C^C[AT][AT]GG
TaqI          359  TCGA                 T^CGA
TthHB8I       359  TCGA                 T^CGA
Van91I        966  CCACCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI          145  GACGTC               G[GA]^CG[CT]C
              606  GGCGCC              
AlwNI         380  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              490  CAGCTCCTG           
ApaI          391  GGGCCC               GGGCC^C
              855  GGGCCC              
AviII         173  TGCGCA               TGC^GCA
             1123  TGCGCA              
BsaHI         145  GACGTC               G[GA]^CG[CT]C
              606  GGCGCC              
BsmBI         567  CGTCTC               CGTCTC(1/5)
              749  GAGACG              
BsmFI         448  GGGAC                GGGAC(10/14)
             1196  GTCCC               
BspMI         170  ACCTGC               ACCTGC(4/8)
              734  ACCTGC              
BsrBI         472  GAGCGG               CCGCTC(-3/-3)
             1164  GAGCGG              
DsaI          253  CCATGG               C^C[GA][CT]GG
              779  CCACGG              
EarI          457  GAAGAG               CTCTTC(1/4)
             1018  GAAGAG              
FspI          173  TGCGCA               TGC^GCA
             1123  TGCGCA              
Hin1I         145  GACGTC               G[GA]^CG[CT]C
              606  GGCGCC              
Ksp632I       457  GAAGAG               CTCTTC(1/4)
             1018  GAAGAG              
ScaI          518  AGTACT               AGT^ACT
             1202  AGTACT              
SexAI         293  ACCAGGT              A^CC[AT]GGT
              404  ACCAGGT             
Tsp45I        902  GTCAC                ^GT[GC]AC
             1149  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BmyI        BpuAI       BsaJI       BseRI       BsiEI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EagI        EclXI       Eco52I      EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       TfiI        TseI        
TspRI       XhoII       
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