Restriction map for pF1KSDA0958
 (Linear) (Six-base) MAPSORT of: KIAA0958.seq  Check: 559  from: 1  to: 1272



KIAA0958.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     743    1272 
   Size:        743     529
 
AatII G_ACGT'C

Cuts at:      0     149    1272 
   Size:        149    1123
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     241    1272 
   Size:        229      12    1031

  Fragments arranged by size: 

                1031     229      12
 
AhdI GACnn_n'nnGTC

Cuts at:      0     557    1272 
   Size:        557     715
 
AlwNI CAG_nnn'CTG

Cuts at:      0     385     495    1272 
   Size:        385     110     777

  Fragments arranged by size: 

                 777     385     110
 
ApaI G_GGCC'C

Cuts at:      0     395     859    1272 
   Size:        395     464     413

  Fragments arranged by size: 

                 464     413     395
 
AvaI C'yCGr_G

Cuts at:      0    1039    1272 
   Size:       1039     233
 
BamHI G'GATC_C

Cuts at:      0     797    1272 
   Size:        797     475
 
BanI G'GyrC_C

Cuts at:      0     606     731    1136    1272 
   Size:        606     125     405     136

  Fragments arranged by size: 

                 606     405     136     125
 
BanII G_rGCy'C

Cuts at:      0     395     592     844     859    1082    1272 
   Size:        395     197     252      15     223     190

  Fragments arranged by size: 

                 395     252     223     197     190      15
 
BbeI G_GCGC'C

Cuts at:      0     610    1272 
   Size:        610     662
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      12     217     229     967    1272 
   Size:         12     205      12     738     305

  Fragments arranged by size: 

                 738     305     205      12      12
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     696    1272 
   Size:        696     576
 
BclI T'GATC_A

Cuts at:      0     486    1272 
   Size:        486     786
 
BlpI GC'TnA_GC

Cuts at:      0     591    1272 
   Size:        591     681
 
Bme1580I G_kGCm'C

Cuts at:      0     395     859    1272 
   Size:        395     464     413

  Fragments arranged by size: 

                 464     413     395
 
BmgBI CAC'GTC

Cuts at:      0    1148    1272 
   Size:       1148     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     708     914    1272 
   Size:        708     206     358

  Fragments arranged by size: 

                 708     358     206
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1096    1272 
   Size:       1096     176
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     544    1272 
   Size:        544     728
 
BsaAI yAC'GTr

Cuts at:      0    1064    1272 
   Size:       1064     208
 
BsaHI Gr'CG_yC

Cuts at:      0     146     607    1272 
   Size:        146     461     665

  Fragments arranged by size: 

                 665     461     146
 
BsaWI w'CCGG_w

Cuts at:      0     299    1272 
   Size:        299     973
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     562    1084    1255    1272 
   Size:        244     318     522     171      17

  Fragments arranged by size: 

                 522     318     244     171      17
 
BseYI C'CCAG_C

Cuts at:      0      30     837    1272 
   Size:         30     807     435

  Fragments arranged by size: 

                 807     435      30
 
BsiEI CG_ry'CG

Cuts at:      0      98     478    1160    1272 
   Size:         98     380     682     112

  Fragments arranged by size: 

                 682     380     112      98
 
BsiHKAI G_wGCw'C

Cuts at:      0    1082    1272 
   Size:       1082     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     573     743    1272 
   Size:        573     170     529

  Fragments arranged by size: 

                 573     529     170
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     592     844     859    1082    1272 
   Size:        395     197     252      15     223     190

  Fragments arranged by size: 

                 395     252     223     197     190      15
 
BspEI T'CCGG_A

Cuts at:      0     299    1272 
   Size:        299     973
 
BspHI T'CATG_A

Cuts at:      0     956    1272 
   Size:        956     316
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     179     743    1272 
   Size:        179     564     529

  Fragments arranged by size: 

                 564     529     179
 
BsrBI CCG'CTC

Cuts at:      0     474    1166    1272 
   Size:        474     692     106

  Fragments arranged by size: 

                 692     474     106
 
BsrFI r'CCGG_y

Cuts at:      0     968    1272 
   Size:        968     304
 
BssSI C'ACGA_G

Cuts at:      0     514    1272 
   Size:        514     758
 
BstYI r'GATC_y

Cuts at:      0     797     944    1118    1272 
   Size:        797     147     174     154

  Fragments arranged by size: 

                 797     174     154     147
 
BtgI C'CryG_G

Cuts at:      0     253     779    1272 
   Size:        253     526     493

  Fragments arranged by size: 

                 526     493     253
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     360    1272 
   Size:        360     912
 
BtsI GCAGTG_nn'

Cuts at:      0      44    1272 
   Size:         44    1228
 
EaeI y'GGCC_r

Cuts at:      0      66      95     475     989    1157    1272 
   Size:         66      29     380     514     168     115

  Fragments arranged by size: 

                 514     380     168     115      66      29
 
EagI C'GGCC_G

Cuts at:      0      95     475    1157    1272 
   Size:         95     380     682     115

  Fragments arranged by size: 

                 682     380     115      95
 
EarI CTCTTCn'nnn_

Cuts at:      0     452    1013    1272 
   Size:        452     561     259

  Fragments arranged by size: 

                 561     452     259
 
EcoICRI GAG'CTC

Cuts at:      0    1080    1272 
   Size:       1080     192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     241    1096    1272 
   Size:        229      12     855     176

  Fragments arranged by size: 

                 855     229     176      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     739    1272 
   Size:        739     533
 
EcoO109I rG'GnC_Cy

Cuts at:      0     392     449     855     856    1272 
   Size:        392      57     406       1     416

  Fragments arranged by size: 

                 416     406     392      57       1
 
FspI TGC'GCA

Cuts at:      0     175    1125    1272 
   Size:        175     950     147

  Fragments arranged by size: 

                 950     175     147
 
HaeII r_GCGC'y

Cuts at:      0     610    1272 
   Size:        610     662
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     543     575     753     785    1153    1185 
   Size:         91      32     420      32     178      32     368      32

Cuts at:   1185    1272 
   Size:         87

  Fragments arranged by size: 

                 420     368     178      91      87      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     150    1272 
   Size:        150    1122
 
KasI G'GCGC_C

Cuts at:      0     606    1272 
   Size:        606     666
 
MscI TGG'CCA

Cuts at:      0     991    1272 
   Size:        991     281
 
NarI GG'CG_CC

Cuts at:      0     607    1272 
   Size:        607     665
 
NcoI C'CATG_G

Cuts at:      0     253    1272 
   Size:        253    1019
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     972    1272 
   Size:        972     300
 
PfoI T'CCnGG_A

Cuts at:      0     806    1272 
   Size:        806     466
 
PmlI CAC'GTG

Cuts at:      0    1064    1272 
   Size:       1064     208
 
PpuMI rG'GwC_Cy

Cuts at:      0     449    1272 
   Size:        449     823
 
PspOMI G'GGCC_C

Cuts at:      0     391     855    1272 
   Size:        391     464     417

  Fragments arranged by size: 

                 464     417     391
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     355     387    1272 
   Size:        355      32     885

  Fragments arranged by size: 

                 885     355      32
 
RsrII CG'GwC_CG

Cuts at:      0     638    1272 
   Size:        638     634
 
SacI G_AGCT'C

Cuts at:      0    1082    1272 
   Size:       1082     190
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1013    1272 
   Size:       1013     259
 
ScaI AGT'ACT

Cuts at:      0     520    1204    1272 
   Size:        520     684      68

  Fragments arranged by size: 

                 684     520      68
 
SexAI A'CCwGG_T

Cuts at:      0     293     404    1272 
   Size:        293     111     868

  Fragments arranged by size: 

                 868     293     111
 
SfcI C'TryA_G

Cuts at:      0     522    1272 
   Size:        522     750
 
SfoI GGC'GCC

Cuts at:      0     608    1272 
   Size:        608     664
 
StyI C'CwwG_G

Cuts at:      0     253    1272 
   Size:        253    1019
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     225    1272 
   Size:        225    1047
 
TatI w'GTAC_w

Cuts at:      0     497     518    1202    1272 
   Size:        497      21     684      70

  Fragments arranged by size: 

                 684     497      70      21
 
ZraI GAC'GTC

Cuts at:      0     147    1272 
   Size:        147    1125
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AhdI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI     BlpI Bme1580I
    BmgBI     BmrI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BssSI    BstYI     BtgI    BtgZI     BtsI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I   HincII     KasI     MscI     NarI     NcoI    PflMI     PfoI
     PmlI    PpuMI   PspOMI     PsrI    RsrII     SacI     SapI     ScaI
    SexAI     SfcI     SfoI     StyI    TaqII     TatI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BglI    BglII     BmtI     BplI
   Bpu10I    BpuEI    BsaBI    BsaXI     BsgI    BsiWI     BsmI    BsrDI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EciI    EcoRI    EcoRV
     FalI     FseI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MslI   MspA1I     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PpiI
    PshAI     PsiI    PspXI     PstI     PvuI    PvuII    SacII     SalI
    SanDI     SbfI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI
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