Restriction map for pF1KSDA0952
 (Linear) (Six-base) MAPSORT of: KIAA0952.seq  Check: 2052  from: 1  to: 1566



KIAA0952.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AccI GT'mk_AC

Cuts at:      0     495    1054    1566 
   Size:        495     559     512

  Fragments arranged by size: 

                 559     512     495
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     899    1063    1566 
   Size:        899     164     503

  Fragments arranged by size: 

                 899     503     164
 
AfeI AGC'GCT

Cuts at:      0    1120    1566 
   Size:       1120     446
 
AflII C'TTAA_G

Cuts at:      0    1282    1566 
   Size:       1282     284
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     148    1566 
   Size:        148    1418
 
AlwNI CAG_nnn'CTG

Cuts at:      0     632    1566 
   Size:        632     934
 
BanII G_rGCy'C

Cuts at:      0     439    1566 
   Size:        439    1127
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     172    1566 
   Size:        172    1394
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     496     530     970    1004    1566 
   Size:        496      34     440      34     562

  Fragments arranged by size: 

                 562     496     440      34      34
 
BglII A'GATC_T

Cuts at:      0     900    1566 
   Size:        900     666
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     882    1366    1532    1566 
   Size:        882     484     166      34

  Fragments arranged by size: 

                 882     484     166      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     667    1227    1566 
   Size:        207     460     560     339

  Fragments arranged by size: 

                 560     460     339     207
 
Bpu10I CC'TnA_GC

Cuts at:      0    1073    1566 
   Size:       1073     493
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751    1335    1566 
   Size:        751     584     231

  Fragments arranged by size: 

                 751     584     231
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     643    1024    1566 
   Size:        643     381     542

  Fragments arranged by size: 

                 643     542     381
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     673     721    1491    1566 
   Size:        673      48     770      75

  Fragments arranged by size: 

                 770     673      75      48
 
BseYI C'CCAG_C

Cuts at:      0     722     731    1115    1566 
   Size:        722       9     384     451

  Fragments arranged by size: 

                 722     451     384       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     986    1396    1566 
   Size:        986     410     170

  Fragments arranged by size: 

                 986     410     170
 
BsiHKAI G_wGCw'C

Cuts at:      0     439     683    1501    1566 
   Size:        439     244     818      65

  Fragments arranged by size: 

                 818     439     244      65
 
BsmI GAATG_Cn'

Cuts at:      0     675     840     892    1566 
   Size:        675     165      52     674

  Fragments arranged by size: 

                 675     674     165      52
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      52    1566 
   Size:         52    1514
 
Bsp1286I G_dGCh'C

Cuts at:      0     439     683    1501    1566 
   Size:        439     244     818      65

  Fragments arranged by size: 

                 818     439     244      65
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     519    1566 
   Size:        519    1047
 
BsrDI GCAATG_nn'

Cuts at:      0    1217    1431    1566 
   Size:       1217     214     135

  Fragments arranged by size: 

                1217     214     135
 
BsrGI T'GTAC_A

Cuts at:      0     363    1566 
   Size:        363    1203
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     997    1566 
   Size:        997     569
 
BstYI r'GATC_y

Cuts at:      0     900    1541    1566 
   Size:        900     641      25

  Fragments arranged by size: 

                 900     641      25
 
BstZ17I GTA'TAC

Cuts at:      0     496    1055    1566 
   Size:        496     559     511

  Fragments arranged by size: 

                 559     511     496
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     346     466    1566 
   Size:        346     120    1100

  Fragments arranged by size: 

                1100     346     120
 
DraIII CAC_nnn'GTG

Cuts at:      0     388    1566 
   Size:        388    1178
 
EaeI y'GGCC_r

Cuts at:      0     581    1566 
   Size:        581     985
 
EcoICRI GAG'CTC

Cuts at:      0     437    1566 
   Size:        437    1129
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     667     899    1063    1227    1566 
   Size:        207     460     232     164     164     339

  Fragments arranged by size: 

                 460     339     232     207     164     164
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     703    1566 
   Size:        703     863
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1104    1566 
   Size:       1104     462
 
HaeII r_GCGC'y

Cuts at:      0    1122    1167    1566 
   Size:       1122      45     399

  Fragments arranged by size: 

                1122     399      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1526    1558    1566 
   Size:       1526      32       8

  Fragments arranged by size: 

                1526      32       8
 
MscI TGG'CCA

Cuts at:      0     583    1566 
   Size:        583     983
 
MslI CAynn'nnrTG

Cuts at:      0     416    1566 
   Size:        416    1150
 
MspA1I CmG'CkG

Cuts at:      0    1247    1566 
   Size:       1247     319
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     388    1566 
   Size:        388    1178
 
PfoI T'CCnGG_A

Cuts at:      0     644    1566 
   Size:        644     922
 
PstI C_TGCA'G

Cuts at:      0    1062    1260    1566 
   Size:       1062     198     306

  Fragments arranged by size: 

                1062     306     198
 
PvuII CAG'CTG

Cuts at:      0    1247    1566 
   Size:       1247     319
 
SacI G_AGCT'C

Cuts at:      0     439    1566 
   Size:        439    1127
 
ScaI AGT'ACT

Cuts at:      0    1324    1566 
   Size:       1324     242
 
SexAI A'CCwGG_T

Cuts at:      0     384    1566 
   Size:        384    1182
 
SfcI C'TryA_G

Cuts at:      0    1058    1256    1566 
   Size:       1058     198     310

  Fragments arranged by size: 

                1058     310     198
 
SmlI C'TyrA_G

Cuts at:      0     766    1282    1314    1566 
   Size:        766     516      32     252

  Fragments arranged by size: 

                 766     516     252      32
 
TatI w'GTAC_w

Cuts at:      0     363     605    1322    1566 
   Size:        363     242     717     244

  Fragments arranged by size: 

                 717     363     244     242
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     846    1413    1566 
   Size:        846     567     153

  Fragments arranged by size: 

                 846     567     153
 
XmnI GAAnn'nnTTC

Cuts at:      0     812    1566 
   Size:        812     754
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII     AlfI    AlwNI    BanII     BbsI
     BcgI    BglII     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI
    BsrGI   BstAPI    BstYI  BstZ17I    BtgZI   DraIII     EaeI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MscI     MslI   MspA1I
    PflMI     PfoI     PstI    PvuII     SacI     ScaI    SexAI     SfcI
     SmlI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI   AflIII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI    BspEI    BspHI
    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI   Bsu36I
     BtgI     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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