################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AclI AflII AgeI AseI AsnI Asp700I AviII AvrII BfrI BlnI BsiWI Bsp1407I BspDI BspLU11I BsrFI BsrGI BstBI Cfr10I ClaI DraIII EagI EclXI Eco52I EcoRV FseI FspI HincII HindII HpaI MfeI MluI MunI NaeI NdeI NgoMIV NotI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI Sse8387I SspBI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 4358 GGTACC G^GTACC AccIII 1985 TCCGGA T^CCGGA AhdI 1931 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3714 GTGCAC G^TGCAC AocI 3929 CCTCAGG CC^T[ACGT]AGG Aor51HI 254 AGCGCT AGC^GCT ApaI 896 GGGCCC GGGCC^C ApaLI 3714 GTGCAC G^TGCAC AscI 48 GGCGCGCC GG^CGCGCC Asp718I 4358 GGTACC G^GTACC AspEI 1931 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3982 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 999 GGCGCC GGCGC^C BbrPI 3085 CACGTG CAC^GTG BbvCI 2164 CCTCAGC CCTCAGC(-5/-2) BciVI 3336 GGATAC GTATCC(6/5) BclI 1685 TGATCA T^GATCA BglII 3371 AGATCT A^GATCT BlpI 2530 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2530 GCTGAGC GC^T[ACGT]AGC BseAI 1985 TCCGGA T^CCGGA BsiEI 799 CGACCG CG[GA][CT]^CG BspEI 1985 TCCGGA T^CCGGA BspHI 1667 TCATGA T^CATGA BsrDI 2729 GCAATG GCAATG(2/0) BssHII 49 GCGCGC G^CGCGC Bst1107I 2603 GTATAC GTA^TAC BstZ17I 2603 GTATAC GTA^TAC Bsu36I 3929 CCTCAGG CC^T[ACGT]AGG CelII 2530 GCTGAGC GC^T[ACGT]AGC CpoI 5 CGGACCG CG^G[AT]CCG DraI 1378 TTTAAA TTT^AAA Eam1105I 1931 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 254 AGCGCT AGC^GCT Eco81I 3929 CCTCAGG CC^T[ACGT]AGG EcoRI 823 GAATTC G^AATTC FbaI 1685 TGATCA T^GATCA HindIII 167 AAGCTT A^AGCTT KasI 999 GGCGCC G^GCGCC KpnI 4358 GGTACC GGTAC^C KspI 3868 CCGCGG CCGC^GG MroI 1985 TCCGGA T^CCGGA NarI 999 GGCGCC GG^CGCC NheI 3438 GCTAGC G^CTAGC NruI 3194 TCGCGA TCG^CGA PflFI 3982 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3085 CACGTG CAC^GTG PmlI 3085 CACGTG CAC^GTG RcaI 1667 TCATGA T^CATGA RsrII 5 CGGACCG CG^G[AT]CCG SacII 3868 CCGCGG CCGC^GG SfoI 999 GGCGCC GGC^GCC SmaI 2016 CCCGGG CCC^GGG SpeI 1321 ACTAGT A^CTAGT Tth111I 3982 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3517 TCTAGA T^CTAGA XmaCI 2016 CCCGGG C^CCGGG XmaI 2016 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 823 GAATTC [GA]^AATT[CT] 4437 AAATTT AflIII 1501 ACGTGT A^C[GA][CT]GT 3683 ACACGT ApoI 823 GAATTC [GA]^AATT[CT] 4437 AAATTT BsaBI 1686 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2517 GATCGTGATC BsaWI 867 ACCGGA [AT]^CCGG[AT] 1985 TCCGGA BsmBI 4169 GAGACG CGTCTC(1/5) 4616 GAGACG BsmI 1844 GAATGC GAATGC(1/-1) 1094 GCATTC BsrBI 313 GAGCGG CCGCTC(-3/-3) 546 GAGCGG BssSI 245 CACGAG CACGAG(-5/-1) 2116 CTCGTG BstAPI 3377 GCAAACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4599 GCAGCGCATGC BstEII 2493 GGTGACC G^GT[ACGT]ACC 4503 GGTGACC BstPI 2493 GGTGACC G^GT[ACGT]ACC 4503 GGTGACC DrdI 2217 GACGGGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4561 GACATCGGCGTC EcoO65I 2493 GGTGACC G^GT[ACGT]ACC 4503 GGTGACC MamI 1686 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2517 GATCGTGATC PflMI 567 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 900 CCAGATGCTGG SacI 718 GAGCTC GAGCT^C 4273 GAGCTC SexAI 2825 ACCTGGT A^CC[AT]GGT 4490 ACCTGGT SmlI 3748 CTCAAG C^T[CT][GA]AG 4200 CTCAAG SphI 4352 GCATGC GCATG^C 4604 GCATGC Van91I 567 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 900 CCAGATGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AatII AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII