Restriction map for pF1KSDA0932
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AccI        AclI        AcsI        AflII       AgeI        
AhdI        AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        AspEI       AspI        AvrII       
BamHI       BbsI        BbvCI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaBI       BsmI        BspDI       BspHI       BssHII      
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HpaI        KspI        MaeI        MamI        MfeI        
MluI        MunI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        2011  GACGTC               GACGT^C
Acc65I       1109  GGTACC               G^GTACC
AflIII        783  ACATGT               A^C[GA][CT]GT
Asp718I      1109  GGTACC               G^GTACC
AviII        2039  TGCGCA               TGC^GCA
BbrPI        1501  CACGTG               CAC^GTG
BcgI         1542  CGACAGCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1917  GTATCC               GTATCC(6/5)
BlpI         2876  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2876  GCTCAGC              GC^T[ACGT]AGC
BsiEI        2892  CGGCCG               CG[GA][CT]^CG
BsiWI        1376  CGTACG               C^GTACG
Bsp1407I     1034  TGTACA               T^GTACA
BspLU11I      783  ACATGT               A^CATGT
BsrBI        1958  CCGCTC               CCGCTC(-3/-3)
BsrGI        1034  TGTACA               T^GTACA
BstEII       1146  GGTCACC              G^GT[ACGT]ACC
BstPI        1146  GGTCACC              G^GT[ACGT]ACC
CelII        2876  GCTCAGC              GC^T[ACGT]AGC
DraI         1195  TTTAAA               TTT^AAA
EagI         2892  CGGCCG               C^GGCCG
EclXI        2892  CGGCCG               C^GGCCG
Eco52I       2892  CGGCCG               C^GGCCG
EcoO65I      1146  GGTCACC              G^GT[ACGT]ACC
FspI         2039  TGCGCA               TGC^GCA
HincII       2242  GTCAAC               GT[CT]^[GA]AC
HindII       2242  GTCAAC               GT[CT]^[GA]AC
KpnI         1109  GGTACC               GGTAC^C
NaeI         1316  GCCGGC               GCC^GGC
NdeI         1553  CATATG               CA^TATG
NgoMIV       1316  GCCGGC               G^CCGGC
PmaCI        1501  CACGTG               CAC^GTG
PmlI         1501  CACGTG               CAC^GTG
SbfI         1062  CCTGCAGG             CCTGCA^GG
SexAI        1092  ACCTGGT              A^CC[AT]GGT
SphI          408  GCATGC               GCATG^C
Sse8387I     1062  CCTGCAGG             CCTGCA^GG
SspBI        1034  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccIII       1440  TCCGGA               T^CCGGA
             2804  TCCGGA              
Alw44I       2327  GTGCAC               G^TGCAC
             2650  GTGCAC              
ApaI           23  GGGCCC               GGGCC^C
             2177  GGGCCC              
ApaLI        2327  GTGCAC               G^TGCAC
             2650  GTGCAC              
BalI          984  TGGCCA               TGG^CCA
             1744  TGGCCA              
BbeI           64  GGCGCC               GGCGC^C
              656  GGCGCC              
BsaAI        1222  TACGTG               [CT]AC^GT[GA]
             1501  CACGTG              
BsaI          776  GAGACC               GGTCTC(1/5)
             1057  GAGACC              
BseAI        1440  TCCGGA               T^CCGGA
             2804  TCCGGA              
BspEI        1440  TCCGGA               T^CCGGA
             2804  TCCGGA              
BsrDI         192  CATTGC               GCAATG(2/0)
             2006  GCAATG              
BsrFI        1316  GCCGGC               [GA]^CCGG[CT]
             1414  GCCGGT              
BssSI         724  CACGAG               CACGAG(-5/-1)
             2293  CACGAG              
BstAPI       2075  GCAGGTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2657  GCACAGAGTGC         
Cfr10I       1316  GCCGGC               [GA]^CCGG[CT]
             1414  GCCGGT              
DraIII       2434  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2658  CACAGAGTG           
HindIII       606  AAGCTT               A^AGCTT
             1520  AAGCTT              
KasI           64  GGCGCC               G^GCGCC
              656  GGCGCC              
MluNI         984  TGGCCA               TGG^CCA
             1744  TGGCCA              
MroI         1440  TCCGGA               T^CCGGA
             2804  TCCGGA              
MscI          984  TGGCCA               TGG^CCA
             1744  TGGCCA              
NarI           64  GGCGCC               GG^CGCC
              656  GGCGCC              
NcoI          332  CCATGG               C^CATGG
             1184  CCATGG              
PleI           74  GACTC                GAGTC(4/5)
              485  GAGTC               
PpuMI         400  GGGACCT              [GA]G^G[AT]CC[CT]
             2353  GGGACCC             
PvuII         142  CAGCTG               CAG^CTG
             1545  CAGCTG              
SapI          295  GAAGAGC              GCTCTTC(1/4)
              443  GAAGAGC             
SfoI           64  GGCGCC               GGC^GCC
              656  GGCGCC              
SmaI            5  CCCGGG               CCC^GGG
              434  CCCGGG              
Tsp509I       829  AATT                 ^AATT
             1714  AATT                
XcmI          251  CCAAGCAGACAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2712  CCACGCCCAGTTTGG     
XmaCI           5  CCCGGG               C^CCGGG
              434  CCCGGG              
XmaI            5  CCCGGG               C^CCGGG
              434  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Asp700I     AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PstI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XhoII       XmnI        
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