Restriction map for pF1KSDA0932
 (Linear) (Six-base) MAPSORT of: KIAA0932.seq  Check: 8476  from: 1  to: 3045



KIAA0932.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1935    2258    2926    3045 
   Size:       1935     323     668     119

  Fragments arranged by size: 

                1935     668     323     119
 
AatII G_ACGT'C

Cuts at:      0    2015    3045 
   Size:       2015    1030
 
Acc65I G'GTAC_C

Cuts at:      0    1109    3045 
   Size:       1109    1936
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1963    2157    2698    2705    3045 
   Size:       1963     194     541       7     340

  Fragments arranged by size: 

                1963     541     340     194       7
 
AflIII A'CryG_T

Cuts at:      0     783    3045 
   Size:        783    2262
 
AleI CACnn'nnGTG

Cuts at:      0     572     574    1613    3045 
   Size:        572       2    1039    1432

  Fragments arranged by size: 

                1432    1039     572       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2143    3045 
   Size:       2143     902
 
AlwNI CAG_nnn'CTG

Cuts at:      0     144     245     632    2081    3045 
   Size:        144     101     387    1449     964

  Fragments arranged by size: 

                1449     964     387     144     101
 
ApaI G_GGCC'C

Cuts at:      0      27    2181    3045 
   Size:         27    2154     864

  Fragments arranged by size: 

                2154     864      27
 
ApaLI G'TGCA_C

Cuts at:      0    2327    2650    3045 
   Size:       2327     323     395

  Fragments arranged by size: 

                2327     395     323
 
AvaI C'yCGr_G

Cuts at:      0       5      76     434    2739    3045 
   Size:          5      71     358    2305     306

  Fragments arranged by size: 

                2305     358     306      71       5
 
BanI G'GyrC_C

Cuts at:      0      64     343     656    1109    3045 
   Size:         64     279     313     453    1936

  Fragments arranged by size: 

                1936     453     313     279      64
 
BanII G_rGCy'C

Cuts at:      0      27     862    1298    1651    2181    2368    2705    3045 
   Size:         27     835     436     353     530     187     337     340

  Fragments arranged by size: 

                 835     530     436     353     340     337     187      27
            
 
BbeI G_GCGC'C

Cuts at:      0      68     660    3045 
   Size:         68     592    2385

  Fragments arranged by size: 

                2385     592      68
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1531    1565    3045 
   Size:       1531      34    1480

  Fragments arranged by size: 

                1531    1480      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1928    3045 
   Size:       1928    1117
 
BlpI GC'TnA_GC

Cuts at:      0    2877    3045 
   Size:       2877     168
 
Bme1580I G_kGCm'C

Cuts at:      0      27     133    1599    2181    2331    2654    3045 
   Size:         27     106    1466     582     150     323     391

  Fragments arranged by size: 

                1466     582     391     323     150     106      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     527     567    2712    2769    3045 
   Size:        527      40    2145      57     276

  Fragments arranged by size: 

                2145     527     276      57      40
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2461    3045 
   Size:       2461     584
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     500    1155    1266    1494    3045 
   Size:        166     334     655     111     228    1551

  Fragments arranged by size: 

                1551     655     334     228     166     111
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     441     903    1004    3045 
   Size:        441     462     101    2041

  Fragments arranged by size: 

                2041     462     441     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     770    1051    3045 
   Size:        770     281    1994

  Fragments arranged by size: 

                1994     770     281
 
BsaAI yAC'GTr

Cuts at:      0    1224    1503    3045 
   Size:       1224     279    1542

  Fragments arranged by size: 

                1542    1224     279
 
BsaHI Gr'CG_yC

Cuts at:      0      65      92     657     968    2012    2060    2095    3045 
   Size:         65      27     565     311    1044      48      35     950

  Fragments arranged by size: 

                1044     950     565     311      65      48      35      27
            
 
BsaWI w'CCGG_w

Cuts at:      0    1440    1967    2804    3045 
   Size:       1440     527     837     241

  Fragments arranged by size: 

                1440     837     527     241
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     414     444    1957    1987    2128    2143    2158    2173 
   Size:        414      30    1513      30     141      15      15      15

Cuts at:   2173    2388    2418    3045 
   Size:        215      30     627

  Fragments arranged by size: 

                1513     627     414     215     141      30      30      30
                  15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139     497     677     680     938    2836    2839    3045 
   Size:        139     358     180       3     258    1898       3     206

  Fragments arranged by size: 

                1898     358     258     206     180     139       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     729     847    1044    1785    2389    3045 
   Size:        729     118     197     741     604     656

  Fragments arranged by size: 

                 741     729     656     604     197     118
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373    1073    2053    2286    2292    2647    2710    3016 
   Size:        373     700     980     233       6     355      63     306

Cuts at:   3016    3045 
   Size:         29

  Fragments arranged by size: 

                 980     700     373     355     306     233      63      29
                   6
 
BsiEI CG_ry'CG

Cuts at:      0    2895    3045 
   Size:       2895     150
 
BsiHKAI G_wGCw'C

Cuts at:      0     862    1651    2331    2654    2705    3045 
   Size:        862     789     680     323      51     340

  Fragments arranged by size: 

                 862     789     680     340     323      51
 
BsiWI C'GTAC_G

Cuts at:      0    1376    3045 
   Size:       1376    1669
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1308    2086    2163    3045 
   Size:       1308     778      77     882

  Fragments arranged by size: 

                1308     882     778      77
 
Bsp1286I G_dGCh'C

Cuts at:      0      27     133     862    1298    1599    1651    2181    2331 
   Size:         27     106     729     436     301      52     530     150

Cuts at:   2331    2368    2654    2705    3045 
   Size:         37     286      51     340

  Fragments arranged by size: 

                 729     530     436     340     301     286     150     106
                  52      51      37      27
 
BspEI T'CCGG_A

Cuts at:      0    1440    2804    3045 
   Size:       1440    1364     241

  Fragments arranged by size: 

                1440    1364     241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1259    1396    1935    2066    2258    2926    3045 
   Size:       1259     137     539     131     192     668     119

  Fragments arranged by size: 

                1259     668     539     192     137     131     119
 
BsrBI CCG'CTC

Cuts at:      0    1960    3045 
   Size:       1960    1085
 
BsrDI GCAATG_nn'

Cuts at:      0     191    2013    3045 
   Size:        191    1822    1032

  Fragments arranged by size: 

                1822    1032     191
 
BsrFI r'CCGG_y

Cuts at:      0    1316    1414    3045 
   Size:       1316      98    1631

  Fragments arranged by size: 

                1631    1316      98
 
BsrGI T'GTAC_A

Cuts at:      0    1034    3045 
   Size:       1034    2011
 
BssSI C'ACGA_G

Cuts at:      0     724    2293    3045 
   Size:        724    1569     752

  Fragments arranged by size: 

                1569     752     724
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2081    2663    3045 
   Size:       2081     582     382

  Fragments arranged by size: 

                2081     582     382
 
BstEII G'GTnAC_C

Cuts at:      0    1146    3045 
   Size:       1146    1899
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     333     742    1615    2299    3045 
   Size:        333     409     873     684     746

  Fragments arranged by size: 

                 873     746     684     409     333
 
BstYI r'GATC_y

Cuts at:      0    1139    1286    1970    3045 
   Size:       1139     147     684    1075

  Fragments arranged by size: 

                1139    1075     684     147
 
BtgI C'CryG_G

Cuts at:      0     332    1184    1307    1583    2573    3045 
   Size:        332     852     123     276     990     472

  Fragments arranged by size: 

                 990     852     472     332     276     123
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1753    3045 
   Size:       1753    1292
 
BtsI GCAGTG_nn'

Cuts at:      0      34    1125    2349    2654    3045 
   Size:         34    1091    1224     305     391

  Fragments arranged by size: 

                1224    1091     391     305      34
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     145     180    3045 
   Size:        145      35    2865

  Fragments arranged by size: 

                2865     145      35
 
DraI TTT'AAA

Cuts at:      0    1197    3045 
   Size:       1197    1848
 
DraIII CAC_nnn'GTG

Cuts at:      0    2439    2663    3045 
   Size:       2439     224     382

  Fragments arranged by size: 

                2439     382     224
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     784     967    2155    3045 
   Size:        784     183    1188     890

  Fragments arranged by size: 

                1188     890     784     183
 
EaeI y'GGCC_r

Cuts at:      0     366     766     984    1605    1744    1826    1906    2892 
   Size:        366     400     218     621     139      82      80     986

Cuts at:   2892    3045 
   Size:        153

  Fragments arranged by size: 

                 986     621     400     366     218     153     139      82
                  80
 
EagI C'GGCC_G

Cuts at:      0    2892    3045 
   Size:       2892     153
 
EarI CTCTTCn'nnn_

Cuts at:      0     290     438    1583    2424    3045 
   Size:        290     148    1145     841     621

  Fragments arranged by size: 

                1145     841     621     290     148
 
EcoICRI GAG'CTC

Cuts at:      0     860    1649    2703    3045 
   Size:        860     789    1054     342

  Fragments arranged by size: 

                1054     860     789     342
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     500    1155    1266    1494    1963    2157    2698 
   Size:        166     334     655     111     228     469     194     541

Cuts at:   2698    2705    3045 
   Size:          7     340

  Fragments arranged by size: 

                 655     541     469     340     334     228     194     166
                 111       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0      23      24     401    2177    2354    3045 
   Size:         23       1     377    1776     177     691

  Fragments arranged by size: 

                1776     691     377     177      23       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1276    1383    2043    2187    3045 
   Size:       1276     107     660     144     858

  Fragments arranged by size: 

                1276     858     660     144     107
 
FspI TGC'GCA

Cuts at:      0    2041    3045 
   Size:       2041    1004
 
HaeII r_GCGC'y

Cuts at:      0      68      87     660    2884    3045 
   Size:         68      19     573    2224     161

  Fragments arranged by size: 

                2224     573     161      68      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     289     321     414     446     473     505    1413    1445 
   Size:        289      32      93      32      27      32     908      32

Cuts at:   1445    1627    1659    2128    2160    3045 
   Size:        182      32     469      32     885

  Fragments arranged by size: 

                 908     885     469     289     182      93      32      32
                  32      32      32      32      27
 
HincII GTy'rAC

Cuts at:      0    2244    3045 
   Size:       2244     801
 
HindIII A'AGCT_T

Cuts at:      0     606    1520    3045 
   Size:        606     914    1525

  Fragments arranged by size: 

                1525     914     606
 
KasI G'GCGC_C

Cuts at:      0      64     656    3045 
   Size:         64     592    2389

  Fragments arranged by size: 

                2389     592      64
 
KpnI G_GTAC'C

Cuts at:      0    1113    3045 
   Size:       1113    1932
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1676    2171    3045 
   Size:       1676     495     874

  Fragments arranged by size: 

                1676     874     495
 
MscI TGG'CCA

Cuts at:      0     986    1746    3045 
   Size:        986     760    1299

  Fragments arranged by size: 

                1299     986     760
 
MslI CAynn'nnrTG

Cuts at:      0     331     572     574    1183    1613    1772    2131    2297 
   Size:        331     241       2     609     430     159     359     166

Cuts at:   2297    3045 
   Size:        748

  Fragments arranged by size: 

                 748     609     430     359     331     241     166     159
                   2
 
MspA1I CmG'CkG

Cuts at:      0      54     144     175    1547    2045    2753    3045 
   Size:         54      90      31    1372     498     708     292

  Fragments arranged by size: 

                1372     708     498     292      90      54      31
 
NaeI GCC'GGC

Cuts at:      0    1318    3045 
   Size:       1318    1727
 
NarI GG'CG_CC

Cuts at:      0      65     657    3045 
   Size:         65     592    2388

  Fragments arranged by size: 

                2388     592      65
 
NcoI C'CATG_G

Cuts at:      0     332    1184    3045 
   Size:        332     852    1861

  Fragments arranged by size: 

                1861     852     332
 
NdeI CA'TA_TG

Cuts at:      0    1554    3045 
   Size:       1554    1491
 
NgoMIV G'CCGG_C

Cuts at:      0    1316    3045 
   Size:       1316    1729
 
NspI r_CATG'y

Cuts at:      0     412     787    2130    3045 
   Size:        412     375    1343     915

  Fragments arranged by size: 

                1343     915     412     375
 
PasI CC'CwG_GG

Cuts at:      0     397    1153    1900    3045 
   Size:        397     756     747    1145

  Fragments arranged by size: 

                1145     756     747     397
 
PciI A'CATG_T

Cuts at:      0     783    3045 
   Size:        783    2262
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     245     982    1051    3045 
   Size:        245     737      69    1994

  Fragments arranged by size: 

                1994     737     245      69
 
PfoI T'CCnGG_A

Cuts at:      0    1232    1289    1571    3020    3045 
   Size:       1232      57     282    1449      25

  Fragments arranged by size: 

                1449    1232     282      57      25
 
PmlI CAC'GTG

Cuts at:      0    1503    3045 
   Size:       1503    1542
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     901     933    3045 
   Size:        901      32    2112

  Fragments arranged by size: 

                2112     901      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     401    2354    3045 
   Size:        401    1953     691

  Fragments arranged by size: 

                1953     691     401
 
PspOMI G'GGCC_C

Cuts at:      0      23    2177    3045 
   Size:         23    2154     868

  Fragments arranged by size: 

                2154     868      23
 
PstI C_TGCA'G

Cuts at:      0    1067    2430    2937    3045 
   Size:       1067    1363     507     108

  Fragments arranged by size: 

                1363    1067     507     108
 
PvuII CAG'CTG

Cuts at:      0     144    1547    3045 
   Size:        144    1403    1498

  Fragments arranged by size: 

                1498    1403     144
 
SacI G_AGCT'C

Cuts at:      0     862    1651    2705    3045 
   Size:        862     789    1054     340

  Fragments arranged by size: 

                1054     862     789     340
 
SanDI GG'GwC_CC

Cuts at:      0    2354    3045 
   Size:       2354     691
 
SapI GCTCTTCn'nnn_

Cuts at:      0     290     438    3045 
   Size:        290     148    2607

  Fragments arranged by size: 

                2607     290     148
 
SbfI CC_TGCA'GG

Cuts at:      0    1067    3045 
   Size:       1067    1978
 
SexAI A'CCwGG_T

Cuts at:      0    1092    3045 
   Size:       1092    1953
 
SfcI C'TryA_G

Cuts at:      0    1063    1449    2426    2933    3045 
   Size:       1063     386     977     507     112

  Fragments arranged by size: 

                1063     977     507     386     112
 
SfoI GGC'GCC

Cuts at:      0      66     658    3045 
   Size:         66     592    2387

  Fragments arranged by size: 

                2387     592      66
 
SmaI CCC'GGG

Cuts at:      0       7     436    3045 
   Size:          7     429    2609

  Fragments arranged by size: 

                2609     429       7
 
SmlI C'TyrA_G

Cuts at:      0     456     918    1019    3045 
   Size:        456     462     101    2026

  Fragments arranged by size: 

                2026     462     456     101
 
SphI G_CATG'C

Cuts at:      0     412    3045 
   Size:        412    2633
 
StyI C'CwwG_G

Cuts at:      0     332     347    1184    1460    2210    3045 
   Size:        332      15     837     276     750     835

  Fragments arranged by size: 

                 837     835     750     332     276      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1134    1314    1892    2751    3045 
   Size:       1134     180     578     859     294

  Fragments arranged by size: 

                1134     859     578     294     180
 
TatI w'GTAC_w

Cuts at:      0    1034    3045 
   Size:       1034    2011
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     684     716     930     962    1082    1114    3045 
   Size:        684      32     214      32     120      32    1931

  Fragments arranged by size: 

                1931     684     214     120      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     258    2719    3045 
   Size:        258    2461     326

  Fragments arranged by size: 

                2461     326     258
 
XmaI C'CCGG_G

Cuts at:      0       5     434    3045 
   Size:          5     429    2611

  Fragments arranged by size: 

                2611     429       5
 
XmnI GAAnn'nnTTC

Cuts at:      0     913    1083    2638    3045 
   Size:        913     170    1555     407

  Fragments arranged by size: 

                1555     913     407     170
 
ZraI GAC'GTC

Cuts at:      0    2013    3045 
   Size:       2013    1032
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI     BanI    BanII     BbeI     BcgI    BciVI
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
  EcoICRI  Eco57MI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI
    SanDI     SapI     SbfI    SexAI     SfcI     SfoI     SmaI     SmlI
     SphI     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AhdI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BclI    BfrBI     BglI    BglII    BmgBI     BmtI   Bpu10I    BsaBI
     BsmI    BspHI   BssHII    BstBI  BstZ17I   Bsu36I     ClaI     EciI
    EcoNI    EcoRI    EcoRV     FseI    FspAI     HpaI     MfeI     MluI
     NheI     NotI     NruI     NsiI     PacI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     ScaI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XhoI
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