Restriction map for pF1KSDA0924
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AcyI        
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        773  GGTACC               G^GTACC
AclI          793  AACGTT               AA^CGTT
Alw44I       1120  GTGCAC               G^TGCAC
AlwI         1515  GATCC                GGATC(4/5)
ApaLI        1120  GTGCAC               G^TGCAC
Asp718I       773  GGTACC               G^GTACC
AspHI        1120  GTGCAC               G[AT]GC[AT]^C
AvaII         935  GGTCC                G^G[AT]CC
BalI         1422  TGGCCA               TGG^CCA
BanII         685  GAGCCC               G[GA]GC[CT]^C
BbsI          283  GAAGAC               GAAGAC(2/6)
BcnI          288  CCGGG                CC^[GC]GG
BpuAI         283  GAAGAC               GAAGAC(2/6)
BsgI         1648  CTGCAC               GTGCAG(16/14)
BsiHKAI      1120  GTGCAC               G[AT]GC[AT]^C
BsiYI        1758  CCAGAGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1758  CCAGAGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I      149  TGTACA               T^GTACA
BspHI         942  TCATGA               T^CATGA
BsrDI         497  GCAATG               GCAATG(2/0)
BsrGI         149  TGTACA               T^GTACA
BstAPI       1122  GCACTCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1479  GGTGACC              G^GT[ACGT]ACC
BstPI        1479  GGTGACC              G^GT[ACGT]ACC
BstXI        1245  CCAGTGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1514  AGATCC               [GA]^GATC[CT]
CfoI          807  GCGC                 GCG^C
DraI         1743  TTTAAA               TTT^AAA
EaeI         1422  TGGCCA               [CT]^GGCC[GA]
EcoO65I      1479  GGTGACC              G^GT[ACGT]ACC
EcoRI         294  GAATTC               G^AATTC
HapII         288  CCGG                 C^CGG
HhaI          807  GCGC                 GCG^C
HinP1I        807  GCGC                 G^CGC
HindIII      1344  AAGCTT               A^AGCTT
HpaII         288  CCGG                 C^CGG
KpnI          773  GGTACC               GGTAC^C
MflI         1514  AGATCC               [GA]^GATC[CT]
MluNI        1422  TGGCCA               TGG^CCA
MscI         1422  TGGCCA               TGG^CCA
MspA1I       1426  CAGCGG               C[AC]G^C[GT]G
MspI          288  CCGG                 C^CGG
NciI          288  CCGGG                CC^[GC]GG
Psp1406I      793  AACGTT               AA^CGTT
RcaI          942  TCATGA               T^CATGA
ScaI         1277  AGTACT               AGT^ACT
SspBI         149  TGTACA               T^GTACA
XhoII        1514  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflII         439  CTTAAG               C^TTAAG
             1450  CTTAAG              
AlwNI          25  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              257  CAGTGTCTG           
Asp700I      1005  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1174  GAAAGGTTTC          
BfrI          439  CTTAAG               C^TTAAG
             1450  CTTAAG              
BseRI         310  GAGGAG               GAGGAG(10/8)
              454  GAGGAG              
BsmI         1134  GCATTC               GAATGC(1/-1)
             1214  GCATTC              
BspMI        1503  ACCTGC               ACCTGC(4/8)
             1646  ACCTGC              
DsaI          963  CCATGG               C^C[GA][CT]GG
             1019  CCATGG              
EcoT14I       963  CCATGG               C^C[AT][AT]GG
             1019  CCATGG              
FauI         1710  CCCGC                CCCGC(4/6)
              672  GCGGG               
MaeII         266  ACGT                 A^CGT
              794  ACGT                
NcoI          963  CCATGG               C^CATGG
             1019  CCATGG              
PleI          429  GACTC                GAGTC(4/5)
              729  GACTC               
SphI          270  GCATGC               GCATG^C
              809  GCATGC              
StyI          963  CCATGG               C^C[AT][AT]GG
             1019  CCATGG              
TaqI         1283  TCGA                 T^CGA
             1441  TCGA                
TfiI          160  GAATC                G^A[AT]TC
             1596  GAATC               
TthHB8I      1283  TCGA                 T^CGA
             1441  TCGA                
XmnI         1005  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1174  GAAAGGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BanI        BbvI        BmyI        BpmI        BsaJI       
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
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