Restriction map for pF1KSDA0924
 (Linear) (Six-base) MAPSORT of: KIAA0924.seq  Check: 475  from: 1  to: 1818



KIAA0924.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1512    1655    1818 
   Size:       1512     143     163

  Fragments arranged by size: 

                1512     163     143
 
Acc65I G'GTAC_C

Cuts at:      0     773    1818 
   Size:        773    1045
 
AclI AA'CG_TT

Cuts at:      0     794    1818 
   Size:        794    1024
 
AflII C'TTAA_G

Cuts at:      0     439    1450    1818 
   Size:        439    1011     368

  Fragments arranged by size: 

                1011     439     368
 
AleI CACnn'nnGTG

Cuts at:      0     145    1818 
   Size:        145    1673
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30     262    1818 
   Size:         30     232    1556

  Fragments arranged by size: 

                1556     232      30
 
ApaLI G'TGCA_C

Cuts at:      0    1120    1818 
   Size:       1120     698
 
ApoI r'AATT_y

Cuts at:      0     294     982     995    1009    1818 
   Size:        294     688      13      14     809

  Fragments arranged by size: 

                 809     688     294      14      13
 
BanI G'GyrC_C

Cuts at:      0     115     773    1781    1818 
   Size:        115     658    1008      37

  Fragments arranged by size: 

                1008     658     115      37
 
BanII G_rGCy'C

Cuts at:      0     689    1818 
   Size:        689    1129
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     290    1818 
   Size:        290    1528
 
Bme1580I G_kGCm'C

Cuts at:      0     751    1124    1229    1818 
   Size:        751     373     105     589

  Fragments arranged by size: 

                 751     589     373     105
 
BmgBI CAC'GTC

Cuts at:      0     267    1818 
   Size:        267    1551
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1537    1818 
   Size:       1537     281
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1160    1328    1679    1703    1818 
   Size:        799     361     168     351      24     115

  Fragments arranged by size: 

                 799     361     351     168     115      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     659    1818 
   Size:        659    1159
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1099    1818 
   Size:       1099     719
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     197     227    1818 
   Size:        197      30    1591

  Fragments arranged by size: 

                1591     197      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     325     469    1818 
   Size:        325     144    1349

  Fragments arranged by size: 

                1349     325     144
 
BseYI C'CCAG_C

Cuts at:      0    1533    1818 
   Size:       1533     285
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1633    1818 
   Size:       1633     185
 
BsiHKAI G_wGCw'C

Cuts at:      0    1124    1818 
   Size:       1124     694
 
BsmI GAATG_Cn'

Cuts at:      0    1134    1214    1818 
   Size:       1134      80     604

  Fragments arranged by size: 

                1134     604      80
 
Bsp1286I G_dGCh'C

Cuts at:      0     689     751    1124    1229    1818 
   Size:        689      62     373     105     589

  Fragments arranged by size: 

                 689     589     373     105      62
 
BspHI T'CATG_A

Cuts at:      0     942    1818 
   Size:        942     876
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1512    1655    1818 
   Size:       1512     143     163

  Fragments arranged by size: 

                1512     163     143
 
BsrDI GCAATG_nn'

Cuts at:      0     504    1818 
   Size:        504    1314
 
BsrGI T'GTAC_A

Cuts at:      0     149    1818 
   Size:        149    1669
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1128    1818 
   Size:       1128     690
 
BstEII G'GTnAC_C

Cuts at:      0    1479    1818 
   Size:       1479     339
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1252    1818 
   Size:       1252     566
 
BstYI r'GATC_y

Cuts at:      0    1514    1818 
   Size:       1514     304
 
BtgI C'CryG_G

Cuts at:      0     963    1019    1818 
   Size:        963      56     799

  Fragments arranged by size: 

                 963     799      56
 
BtsI GCAGTG_nn'

Cuts at:      0     263     456    1818 
   Size:        263     193    1362

  Fragments arranged by size: 

                1362     263     193
 
DraI TTT'AAA

Cuts at:      0    1745    1818 
   Size:       1745      73
 
EaeI y'GGCC_r

Cuts at:      0    1422    1818 
   Size:       1422     396
 
EarI CTCTTCn'nnn_

Cuts at:      0     311     417     455     461     473     637     713    1363 
   Size:        311     106      38       6      12     164      76     650

Cuts at:   1363    1616    1818 
   Size:        253     202

  Fragments arranged by size: 

                 650     311     253     202     164     106      76      38
                  12       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1160    1328    1679    1703    1818 
   Size:        799     361     168     351      24     115

  Fragments arranged by size: 

                 799     361     351     168     115      24
 
EcoRI G'AATT_C

Cuts at:      0     294    1818 
   Size:        294    1524
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1296    1818 
   Size:       1296     522
 
HindIII A'AGCT_T

Cuts at:      0    1344    1818 
   Size:       1344     474
 
KpnI G_GTAC'C

Cuts at:      0     777    1818 
   Size:        777    1041
 
MscI TGG'CCA

Cuts at:      0    1424    1818 
   Size:       1424     394
 
MslI CAynn'nnrTG

Cuts at:      0     145     196     215     968    1071    1818 
   Size:        145      51      19     753     103     747

  Fragments arranged by size: 

                 753     747     145     103      51      19
 
MspA1I CmG'CkG

Cuts at:      0    1428    1818 
   Size:       1428     390
 
NcoI C'CATG_G

Cuts at:      0     963    1019    1818 
   Size:        963      56     799

  Fragments arranged by size: 

                 963     799      56
 
NspI r_CATG'y

Cuts at:      0      55     274     813    1065    1076    1449    1818 
   Size:         55     219     539     252      11     373     369

  Fragments arranged by size: 

                 539     373     369     252     219      55      11
 
PasI CC'CwG_GG

Cuts at:      0     752    1818 
   Size:        752    1066
 
PfoI T'CCnGG_A

Cuts at:      0     845    1818 
   Size:        845     973
 
ScaI AGT'ACT

Cuts at:      0    1279    1818 
   Size:       1279     539
 
SmlI C'TyrA_G

Cuts at:      0     439    1114    1450    1818 
   Size:        439     675     336     368

  Fragments arranged by size: 

                 675     439     368     336
 
SphI G_CATG'C

Cuts at:      0     274     813    1818 
   Size:        274     539    1005

  Fragments arranged by size: 

                1005     539     274
 
StyI C'CwwG_G

Cuts at:      0     963    1019    1818 
   Size:        963      56     799

  Fragments arranged by size: 

                 963     799      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1673    1688    1818 
   Size:       1673      15     130

  Fragments arranged by size: 

                1673     130      15
 
TatI w'GTAC_w

Cuts at:      0     149    1184    1277    1818 
   Size:        149    1035      93     541

  Fragments arranged by size: 

                1035     541     149      93
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1493    1525    1530    1562    1818 
   Size:       1493      32       5      32     256

  Fragments arranged by size: 

                1493     256      32      32       5
 
XmnI GAAnn'nnTTC

Cuts at:      0    1009    1178    1818 
   Size:       1009     169     640

  Fragments arranged by size: 

                1009     640     169
 

 Enzymes that do cut:

     AarI   Acc65I     AclI    AflII     AleI    AlwNI    ApaLI     ApoI
     BanI    BanII     BbsI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI   BstAPI   BstEII    BstXI    BstYI
     BtgI     BtsI     DraI     EaeI     EarI  Eco57MI    EcoRI     FalI
  HindIII     KpnI     MscI     MslI   MspA1I     NcoI     NspI     PasI
     PfoI     ScaI     SmlI     SphI     StyI    TaqII     TatI     TstI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AcuI     AfeI   AflIII     AgeI     AhdI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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