Restriction map for
pF1KSDA0895
(Linear) (Six-base) MAPSORT of: KIAA0895.seq Check: 474 from: 1 to: 1407
KIAA0895.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 1175 1407
Size: 1175 232
AflII C'TTAA_G
Cuts at: 0 706 1407
Size: 706 701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 68 454 1407
Size: 68 386 953
Fragments arranged by size:
953 386 68
ApoI r'AATT_y
Cuts at: 0 550 1187 1407
Size: 550 637 220
Fragments arranged by size:
637 550 220
AvaI C'yCGr_G
Cuts at: 0 126 746 1243 1407
Size: 126 620 497 164
Fragments arranged by size:
620 497 164 126
BanI G'GyrC_C
Cuts at: 0 312 470 1407
Size: 312 158 937
Fragments arranged by size:
937 312 158
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 1407
Size: 215 1192
BbvCI CC'TCA_GC
Cuts at: 0 503 832 1407
Size: 503 329 575
Fragments arranged by size:
575 503 329
BclI T'GATC_A
Cuts at: 0 89 1407
Size: 89 1318
BglII A'GATC_T
Cuts at: 0 1398 1407
Size: 1398 9
BlpI GC'TnA_GC
Cuts at: 0 677 1407
Size: 677 730
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1328 1407
Size: 1328 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 367 841 862 1407
Size: 25 342 474 21 545
Fragments arranged by size:
545 474 342 25 21
Bpu10I CC'TnA_GC
Cuts at: 0 41 503 832 1407
Size: 41 462 329 575
Fragments arranged by size:
575 462 329 41
BsaAI yAC'GTr
Cuts at: 0 808 1407
Size: 808 599
BsaBI GATnn'nnATC
Cuts at: 0 1268 1407
Size: 1268 139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1000 1407
Size: 1000 407
BseYI C'CCAG_C
Cuts at: 0 256 1407
Size: 256 1151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1407
Size: 788 619
BsmI GAATG_Cn'
Cuts at: 0 833 959 1407
Size: 833 126 448
Fragments arranged by size:
833 448 126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 746 1064 1407
Size: 746 318 343
Fragments arranged by size:
746 343 318
BstYI r'GATC_y
Cuts at: 0 288 1398 1407
Size: 288 1110 9
Fragments arranged by size:
1110 288 9
BstZ17I GTA'TAC
Cuts at: 0 1176 1407
Size: 1176 231
BtgI C'CryG_G
Cuts at: 0 886 1329 1407
Size: 886 443 78
Fragments arranged by size:
886 443 78
BtsI GCAGTG_nn'
Cuts at: 0 333 800 1407
Size: 333 467 607
Fragments arranged by size:
607 467 333
DraI TTT'AAA
Cuts at: 0 1062 1407
Size: 1062 345
EaeI y'GGCC_r
Cuts at: 0 1332 1407
Size: 1332 75
EarI CTCTTCn'nnn_
Cuts at: 0 491 1407
Size: 491 916
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 367 841 862 1407
Size: 25 342 474 21 545
Fragments arranged by size:
545 474 342 25 21
EcoO109I rG'GnC_Cy
Cuts at: 0 735 1086 1308 1407
Size: 735 351 222 99
Fragments arranged by size:
735 351 222 99
EcoRI G'AATT_C
Cuts at: 0 1187 1407
Size: 1187 220
EcoRV GAT'ATC
Cuts at: 0 411 1407
Size: 411 996
HindIII A'AGCT_T
Cuts at: 0 28 516 1407
Size: 28 488 891
Fragments arranged by size:
891 488 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 877 1407
Size: 802 75 530
Fragments arranged by size:
802 530 75
MslI CAynn'nnrTG
Cuts at: 0 16 1366 1407
Size: 16 1350 41
Fragments arranged by size:
1350 41 16
MspA1I CmG'CkG
Cuts at: 0 507 1407
Size: 507 900
NcoI C'CATG_G
Cuts at: 0 886 1329 1407
Size: 886 443 78
Fragments arranged by size:
886 443 78
PmlI CAC'GTG
Cuts at: 0 808 1407
Size: 808 599
PpuMI rG'GwC_Cy
Cuts at: 0 735 1086 1308 1407
Size: 735 351 222 99
Fragments arranged by size:
735 351 222 99
PstI C_TGCA'G
Cuts at: 0 1325 1407
Size: 1325 82
PvuII CAG'CTG
Cuts at: 0 507 1407
Size: 507 900
SanDI GG'GwC_CC
Cuts at: 0 1308 1407
Size: 1308 99
SbfI CC_TGCA'GG
Cuts at: 0 1325 1407
Size: 1325 82
ScaI AGT'ACT
Cuts at: 0 437 1407
Size: 437 970
SexAI A'CCwGG_T
Cuts at: 0 1169 1407
Size: 1169 238
SfcI C'TryA_G
Cuts at: 0 181 1321 1407
Size: 181 1140 86
Fragments arranged by size:
1140 181 86
SmlI C'TyrA_G
Cuts at: 0 706 1407
Size: 706 701
SpeI A'CTAG_T
Cuts at: 0 373 1407
Size: 373 1034
StyI C'CwwG_G
Cuts at: 0 886 1329 1407
Size: 886 443 78
Fragments arranged by size:
886 443 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 713 1407
Size: 713 694
TatI w'GTAC_w
Cuts at: 0 435 1407
Size: 435 972
XbaI T'CTAG_A
Cuts at: 0 511 1407
Size: 511 896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 893 1407
Size: 893 514
XmnI GAAnn'nnTTC
Cuts at: 0 29 111 1407
Size: 29 82 1296
Fragments arranged by size:
1296 82 29
Enzymes that do cut:
AccI AflII AlfI ApoI AvaI BanI BbsI BbvCI
BclI BglII BlpI BplI BpmI Bpu10I BsaAI BsaBI
BseRI BseYI BsgI BsmI BspMI BstYI BstZ17I BtgI
BtsI DraI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV
HindIII MmeI MslI MspA1I NcoI PmlI PpuMI PstI
PvuII SanDI SbfI ScaI SexAI SfcI SmlI SpeI
StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BcgI BciVI
BfrBI BglI Bme1580I BmgBI BmrI BmtI BpuEI BsaI
BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI
SwaI TstI Tth111I XhoI XmaI ZraI