Restriction map for pF1KSDA0887
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
AlwNI       Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BanII       BbrPI       BbvCI       
BcgI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BseAI       
BsgI        BsiEI       BsiWI       BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
ScaI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SspI        SwaI        Tth111I     XbaI        
XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AcsI         1011  GAATTT               [GA]^AATT[CT]
AcyI            6  GGCGCC               G[GA]^CG[CT]C
Alw44I        581  GTGCAC               G^TGCAC
ApaLI         581  GTGCAC               G^TGCAC
ApoI         1011  GAATTT               [GA]^AATT[CT]
Asp700I      1195  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         581  GTGCAC               G[AT]GC[AT]^C
AvaI         1115  CTCGAG               C^[CT]CG[GA]G
AvaII        1320  GGACC                G^G[AT]CC
BalI          698  TGGCCA               TGG^CCA
BanI            6  GGCGCC               G^G[CT][GA]CC
BbeI            6  GGCGCC               GGCGC^C
BbsI          457  GTCTTC               GAAGAC(2/6)
BciVI         652  GGATAC               GTATCC(6/5)
BclI          540  TGATCA               T^GATCA
BcnI          381  CCGGG                CC^[GC]GG
BglI          886  GCCTACCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         457  GTCTTC               GAAGAC(2/6)
BsaHI           6  GGCGCC               G[GA]^CG[CT]C
BsaI          660  GGTCTC               GGTCTC(1/5)
BsaWI         308  TCCGGT               [AT]^CCGG[AT]
BsiHKAI       581  GTGCAC               G[AT]GC[AT]^C
BsmI         1042  GAATGC               GAATGC(1/-1)
BsoBI        1115  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      138  TGTACA               T^GTACA
BspMI         207  GCAGGT               ACCTGC(4/8)
BsrGI         138  TGTACA               T^GTACA
CfoI            7  GCGC                 GCG^C
DraII        1319  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        575  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          698  TGGCCA               [CT]^GGCC[GA]
EcoO109I     1319  AGGACCT              [GA]G^G[ACGT]CC[CT]
FbaI          540  TGATCA               T^GATCA
HaeII           6  GGCGCC               [GA]GCGC^[CT]
HhaI            7  GCGC                 GCG^C
Hin1I           6  GGCGCC               G[GA]^CG[CT]C
HinP1I          7  GCGC                 G^CGC
HphI          543  TCACC                GGTGA(8/7)
KasI            6  GGCGCC               G^GCGCC
MaeII         472  ACGT                 A^CGT
MaeIII        385  GTCAC                ^GT[ACGT]AC
MluNI         698  TGGCCA               TGG^CCA
MscI          698  TGGCCA               TGG^CCA
MspA1I        218  CAGCTG               C[AC]G^C[GT]G
NarI            6  GGCGCC               GG^CGCC
NciI          381  CCGGG                CC^[GC]GG
NlaIV           6  GGCGCC               GG[ACGT]^[ACGT]CC
NspI          628  GCATGC               [GA]CATG^[CT]
PaeR7I       1115  CTCGAG               C^TCGAG
PflMI          99  CCATACCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1319  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         218  CAGCTG               CAG^CTG
SbfI          204  CCTGCAGG             CCTGCA^GG
SexAI         815  ACCTGGT              A^CC[AT]GGT
SfiI          885  GGCCTACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI            6  GGCGCC               GGC^GCC
SphI          628  GCATGC               GCATG^C
Sse8387I      204  CCTGCAGG             CCTGCA^GG
SspBI         138  TGTACA               T^GTACA
Tsp45I        385  GTCAC                ^GT[GC]AC
Van91I         99  CCATACCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1115  CTCGAG               C^TCGAG
XmnI         1195  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AocI           10  CCTGAGG              CC^T[ACGT]AGG
              646  CCTGAGG             
BmyI          446  GGGCAC               G[AGT]GC[ACT]^C
              581  GTGCAC              
BsmBI         999  GAGACG               CGTCTC(1/5)
             1125  GAGACG              
BsmFI         401  GGGAC                GGGAC(10/14)
              738  GGGAC               
Bsp1286I      446  GGGCAC               G[AGT]GC[ACT]^C
              581  GTGCAC              
BsrBI          16  GAGCGG               CCGCTC(-3/-3)
              940  GAGCGG              
BsrI           70  ACTGG                ACTGG(1/-1)
              119  ACTGG               
Bsu36I         10  CCTGAGG              CC^T[ACGT]AGG
              646  CCTGAGG             
Cac8I         628  GCATGC               GC[ACGT]^[ACGT]GC
             1046  GCCTGC              
Cfr13I       1259  GGCCC                G^G[ACGT]CC
             1320  GGACC               
Eco81I         10  CCTGAGG              CC^T[ACGT]AGG
              646  CCTGAGG             
EcoT14I       104  CCTTGG               C^C[AT][AT]GG
              262  CCAAGG              
FauI           18  GCGGG                CCCGC(4/6)
              942  GCGGG               
PleI          550  GACTC                GAGTC(4/5)
             1289  GACTC               
PstI           52  CTGCAG               CTGCA^G
              205  CTGCAG              
SapI          349  GCTCTTC              GCTCTTC(1/4)
              618  GCTCTTC             
Sau96I       1259  GGCCC                G^G[ACGT]CC
             1320  GGACC               
SmlI          249  CTCAAG               C^T[CT][GA]AG
             1115  CTCGAG              
StuI          750  AGGCCT               AGG^CCT
              884  AGGCCT              
StyI          104  CCTTGG               C^C[AT][AT]GG
              262  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BfaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BssKI       BstF5I      BstNI       BstYI       
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
ItaI        Ksp632I     MaeI        MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NdeII       NlaIII      RsaI        
Sau3AI      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
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